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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000292-TA|BGIBMGA000292-PA|IPR013027|FAD-dependent
pyridine nucleotide-disulphide oxidoreductase,
IPR006258|Dihydrolipoamide dehydrogenase, IPR012999|Pyridine
nucleotide-disulphide oxidoreductase, class I, active site,
IPR001327|Pyridine nucleotide-disulphide oxidoreductase, NAD-binding
region, IPR000815|Mercuric reductase, IPR004099|Pyridine
nucleotide-disulphide oxidoreductase dimerisation region,
IPR001100|Pyridine nucleotide-disulphide oxidoreductase, class I
         (496 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase Dld1|Schi...   567   e-162
SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces pom...   160   5e-40
SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolo...    53   8e-08
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ...    37   0.007
SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc...    31   0.37 
SPBC3F6.03 |trr1|caf4|thioredoxin reductase Trr1|Schizosaccharom...    31   0.49 
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual        30   0.85 
SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1 |Schizosacch...    29   1.1  
SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr ...    29   1.1  
SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic enzyme|Schizosaccha...    29   2.0  
SPCC1450.07c |||D-amino acid oxidase |Schizosaccharomyces pombe|...    28   2.6  
SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubi...    28   2.6  
SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica...    28   3.4  
SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate transa...    27   6.0  
SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe...    27   7.9  

>SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase
           Dld1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 511

 Score =  567 bits (1400), Expect = e-162
 Identities = 268/479 (55%), Positives = 352/479 (73%), Gaps = 3/479 (0%)

Query: 20  RIATRQYATTHDADLVVIGSGPGGYVAAIKAAQLGMKVVSVEKDPTLGGTCLNVGCIPSK 79
           R+ + + +   + DL VIG GPGGYVAAI+ AQLG+K + VEK  TLGGTCLNVGCIPSK
Sbjct: 34  RLYSAKASGNGEYDLCVIGGGPGGYVAAIRGAQLGLKTICVEKRGTLGGTCLNVGCIPSK 93

Query: 80  ALLHNSHLYHMAKHDFKQRGIETGEVTFDFKKMMEYKANAVKGLTGGIAMLFQKNKVNLV 139
           ALL+NSH+YH  KHD K+RGI+   V+ +  +MM+ K ++VK LT GI  LF+KNKV   
Sbjct: 94  ALLNNSHIYHTVKHDTKRRGIDVSGVSVNLSQMMKAKDDSVKSLTSGIEYLFKKNKVEYA 153

Query: 140 KGVGTIVAPNKVEVHGEKGV--ETVNTKNILIASGSEVTPFPGVTFDEKQIITSTGALSL 197
           KG G+ + P  + V G  G   +T+  KN +IA+GSEV PFPGVT DEK+I++STGALSL
Sbjct: 154 KGTGSFIDPQTLSVKGIDGAADQTIKAKNFIIATGSEVKPFPGVTIDEKKIVSSTGALSL 213

Query: 198 ESVPKKMLVIGAGVIGLELGSVYQRLGADVTAIEFLTSIGGVGIDGEVAKTLQKILSKQG 257
             VPKKM V+G G+IGLE+GSV+ RLGA+VT +EFL ++GG  +D +++K L +I+SKQG
Sbjct: 214 SEVPKKMTVLGGGIIGLEMGSVWSRLGAEVTVVEFLPAVGGP-MDADISKALSRIISKQG 272

Query: 258 MKFKLGTKVLGVKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRRPYTKGLGLDKVGIA 317
           +KFK  TK+L  K  G +++V++E  K   +E    DV+L++IGR PYT+GLGLDK+GI+
Sbjct: 273 IKFKTSTKLLSAKVNGDSVEVEIENMKNNKRETYQTDVLLVAIGRVPYTEGLGLDKLGIS 332

Query: 318 LDDRGRIPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGIVCVEGIKGMPVHFNYDAIP 377
           +D   R+ ++++++T +P I  IGD   GPMLAHKAEDEGI  VE I     H NY+ IP
Sbjct: 333 MDKSNRVIMDSEYRTNIPHIRVIGDATLGPMLAHKAEDEGIAAVEYIAKGQGHVNYNCIP 392

Query: 378 SVIYTSPEVGWVGKTEEDLKKEGRAYKVGKFPFLANSRAKTNGETEGFVKVLSDKTTDVI 437
           +V+YT PEV WVG TE+  K+ G  Y++G FPF ANSRAKTN + +G VKV+ D  TD +
Sbjct: 393 AVMYTHPEVAWVGITEQKAKESGIKYRIGTFPFSANSRAKTNMDADGLVKVIVDAETDRL 452

Query: 438 LGTHIIGPGGGELINEAVLAQEYGAAAEDVARVCHAHPTCAEALREANLAAYSGKPINF 496
           LG H+IGP  GELI EA LA EYGA+AEDVARVCHAHPT +EA +EA +AA+ GK I+F
Sbjct: 453 LGVHMIGPMAGELIGEATLALEYGASAEDVARVCHAHPTLSEATKEAMMAAWCGKSIHF 511


>SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 464

 Score =  160 bits (388), Expect = 5e-40
 Identities = 137/466 (29%), Positives = 216/466 (46%), Gaps = 31/466 (6%)

Query: 33  DLVVIGSGPGGYVAAIKAAQLGMKVVSVEKDPTLGGTCLNVGCIPSKALLHNSHLYHMAK 92
           D +VIG G GG  +A +AA+ G KV  +E    LGGTC+N GC+P K + + + L    K
Sbjct: 9   DYLVIGGGSGGLASARRAAKHGAKVALIEASGRLGGTCVNYGCVPKKIMWNIADLVAKMK 68

Query: 93  HDFKQRGIETGEV-TFDFKKMMEYKANAVKGLTGGI-AMLFQKNKVNLVKGVGTIVAPNK 150
              KQ G    ++ +FD+  M++ K +A  G   GI      K+ V  + G  + V+P +
Sbjct: 69  -TAKQNGFPNSQLGSFDW-GMIKRKRDAYIGRLNGIYERNVNKDGVAYISGHASFVSPTE 126

Query: 151 --VEVHGEKGVETVNTKNILIA-SGSEVTP--FPGVTFDEKQIITSTGALSLESVPKKML 205
             V+++   G +  + K ILIA  G  + P   PG  +     I S G   LES PK++ 
Sbjct: 127 VAVDMNDGSGTQVFSAKYILIAVGGHPIWPSHIPGAEYG----IDSDGFFELESQPKRVA 182

Query: 206 VIGAGVIGLELGSVYQRLGAD----VTAIEFLTSIGGVGIDGEVAKTLQKILSKQGMKFK 261
           ++GAG I +EL  V+  LG +    +   +FL     +  DG +    Q I         
Sbjct: 183 IVGAGYIAVELAGVFAALGTETHMFIRQSKFLRKFDPIISDG-IMDHFQHI------GIN 235

Query: 262 LGTKVLGVKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRRPYTKGLGLDKVGIALDDR 321
           + T  L  KK       ++      +    + D +L +IGR P  +GL L+K G+     
Sbjct: 236 VHTNSLEFKKVEKLPSGEL-CIHQQDGSTFNVDTLLWAIGRAPKIQGLRLEKAGVKTLPN 294

Query: 322 GRIPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGIVCVEGIKG--MPVHFNYDAIPSV 379
           G I  +   +T VP + ++GDV     L   A   G    + + G     H +Y+ +PSV
Sbjct: 295 GIIIADTYQRTNVPTVLSLGDVCGKLELTPVAIAAGRRLSDRLFGGIKDAHLDYEEVPSV 354

Query: 380 IYTSPEVGWVGKTEED-LKKEGRA-YKV--GKFPFLANSRAKTNGETEGFVKVLSDKTTD 435
           ++  PE G +G TE++ + K G +  KV   KF  L  S  +   +     K++      
Sbjct: 355 VFAHPEAGTIGLTEQEAIDKYGESQIKVYNTKFNGLNYSMVEQEDKVPTTYKLVCAGPLQ 414

Query: 436 VILGTHIIGPGGGELINEAVLAQEYGAAAEDVARVCHAHPTCAEAL 481
            ++G H++G    E++    +A + GA   D       HPT AE L
Sbjct: 415 KVVGLHLVGDFSAEILQGFGVAIKMGATKSDFDSCVAIHPTSAEEL 460


>SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolog
           Aif1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 575

 Score = 53.2 bits (122), Expect = 8e-08
 Identities = 49/198 (24%), Positives = 90/198 (45%), Gaps = 18/198 (9%)

Query: 161 TVNTKNILIASGSEVTPFPGVTFDEKQII--------TSTGALSLESVPKK-MLVIGAGV 211
           T +   +++A+G E    P    D K +         +   A++ E+  KK +++IG+  
Sbjct: 252 TESYTKLILATGGEPNKLPIPGLDSKNVYLLRSIADASKLAAVTTEAGDKKNIVIIGSSF 311

Query: 212 IGLELGSVYQRLGADVTAIEFLTSIGGVGIDGEVAKTLQKILSKQGMKFKLGTKVLGVK- 270
           IGLEL  V +     V  +E +     +G   EV   L+ +  + G+ F L   +  VK 
Sbjct: 312 IGLELAVVLKDHNVSVIGMESIPFEKVMG--KEVGTALKALHEQNGIAFYLENSIKEVKT 369

Query: 271 KEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRRPYTKGLGLDKVGIALDDRGRIPVNNKF 330
               + K +    K G  + +  DVV+++ G +P  + LG     ++L+  G + V+   
Sbjct: 370 SSNDSSKAEHIVLKDG--QSIPADVVILAAGVKPNLRYLG---NAVSLEKDGGVKVDEHC 424

Query: 331 QTT-VPGIYAIGDVIHGP 347
           +      +YA+GD+ H P
Sbjct: 425 RVLGAEDVYAVGDIAHAP 442


>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2111

 Score = 36.7 bits (81), Expect = 0.007
 Identities = 26/83 (31%), Positives = 39/83 (46%), Gaps = 10/83 (12%)

Query: 287  NKEVLDCDVVLISIG----RRPYTKGLGLDKVGIALDDRGRIPVNNK-FQTTVPGIYAIG 341
            ++E    D+V++++G        T G+ +D+     D R  I    K ++T+VPGIYA G
Sbjct: 2011 SEEFFPADLVILALGFLGPEEQATAGMNVDR-----DARSNISTPTKSYETSVPGIYAAG 2065

Query: 342  DVIHGPMLAHKAEDEGIVCVEGI 364
            D   G  L      EG  C   I
Sbjct: 2066 DCRRGQSLVVWGIQEGRQCAREI 2088



 Score = 27.9 bits (59), Expect = 3.4
 Identities = 14/35 (40%), Positives = 19/35 (54%)

Query: 34   LVVIGSGPGGYVAAIKAAQLGMKVVSVEKDPTLGG 68
            + +IGSGP G  AA +  + G  VV  E+    GG
Sbjct: 1758 VAIIGSGPAGLAAADQLNRAGHHVVIYERADRPGG 1792


>SPBC713.12 |erg1||squalene monooxygenase Erg1
          |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 457

 Score = 31.1 bits (67), Expect = 0.37
 Identities = 13/35 (37%), Positives = 21/35 (60%)

Query: 29 THDADLVVIGSGPGGYVAAIKAAQLGMKVVSVEKD 63
          T DAD+++IG+G  G        + G KV+ +E+D
Sbjct: 3  TQDADIIIIGAGITGCALGAALGRQGRKVLVLERD 37



 Score = 27.1 bits (57), Expect = 6.0
 Identities = 11/28 (39%), Positives = 17/28 (60%)

Query: 204 MLVIGAGVIGLELGSVYQRLGADVTAIE 231
           +++IGAG+ G  LG+   R G  V  +E
Sbjct: 8   IIIIGAGITGCALGAALGRQGRKVLVLE 35


>SPBC3F6.03 |trr1|caf4|thioredoxin reductase
           Trr1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 322

 Score = 30.7 bits (66), Expect = 0.49
 Identities = 23/58 (39%), Positives = 31/58 (53%), Gaps = 6/58 (10%)

Query: 289 EVLDCDV--VLISIGRRPYTKGLGLDKVGIALDDRGRIP-VNNKFQTTVPGIYAIGDV 343
           EV D  V  +  +IG  P TK   L    I LD+ G I  +N   +T++PG +A GDV
Sbjct: 235 EVSDLQVNGLFYAIGHIPATK---LVAEQIELDEAGYIKTINGTPRTSIPGFFAAGDV 289



 Score = 30.3 bits (65), Expect = 0.64
 Identities = 16/33 (48%), Positives = 22/33 (66%), Gaps = 1/33 (3%)

Query: 29 THDADLVVIGSGPGGYVAAIKAAQLGMKVVSVE 61
          TH+  +V+IGSGP G+ AAI  A+  +K V  E
Sbjct: 2  THNK-VVIIGSGPAGHTAAIYLARGELKPVMYE 33


>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1279

 Score = 29.9 bits (64), Expect = 0.85
 Identities = 26/78 (33%), Positives = 36/78 (46%), Gaps = 11/78 (14%)

Query: 374 DAIPSVIY-TSPE----VGWVGKTEEDLKKEGR-AYKVGKFPFLANSRAKTNGETEGFVK 427
           DA P+++  T P     V WV KT  +    G  +    K PF A+SRA +   T     
Sbjct: 85  DAFPTLLSKTGPSKPRIVSWVRKTASNTSVAGSDSVSRDKIPFSASSRASSTKST----- 139

Query: 428 VLSDKTTDVILGTHIIGP 445
           + S K TD +  T I+ P
Sbjct: 140 LSSVKETDFVTETLILSP 157


>SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 469

 Score = 29.5 bits (63), Expect = 1.1
 Identities = 17/39 (43%), Positives = 20/39 (51%), Gaps = 1/39 (2%)

Query: 310 GLDKVGIALDDRGRIPVNNKFQTTVPGIYAIGDVIHGPM 348
           G+  VG+  DD   I V N      PGIY  G V HGP+
Sbjct: 344 GMKDVGVPFDDAKGI-VKNVNGFVRPGIYTSGWVKHGPI 381


>SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr
          1|||Manual
          Length = 513

 Score = 29.5 bits (63), Expect = 1.1
 Identities = 16/58 (27%), Positives = 25/58 (43%)

Query: 11 PTFRSGSLVRIATRQYATTHDADLVVIGSGPGGYVAAIKAAQLGMKVVSVEKDPTLGG 68
          P+  S S         +T ++   +VIG G  G  A      LG  V+ ++K+   GG
Sbjct: 18 PSTNSASFFSTLRFNMSTANNTQAIVIGGGLAGLSATNTILDLGGNVLLLDKNTAFGG 75


>SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic
           enzyme|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 328

 Score = 28.7 bits (61), Expect = 2.0
 Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 6/61 (9%)

Query: 14  RSGSLVRIATRQYATTHD----ADLVVIGSGPGGYVAA--IKAAQLGMKVVSVEKDPTLG 67
           R  ++ R  TR+Y +  D    +D+V++G+G  G  AA  I   +  +K+  +E     G
Sbjct: 55  RESTVSRAMTRRYFSDLDKYAESDIVIVGAGSAGLTAAYYIGTRRPDLKIAIIEASVAPG 114

Query: 68  G 68
           G
Sbjct: 115 G 115


>SPCC1450.07c |||D-amino acid oxidase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 348

 Score = 28.3 bits (60), Expect = 2.6
 Identities = 11/30 (36%), Positives = 20/30 (66%)

Query: 195 LSLESVPKKMLVIGAGVIGLELGSVYQRLG 224
           ++ E+ P+ ++++GAGVIGL    +   LG
Sbjct: 1   MTKENKPRDIVIVGAGVIGLTTAWILSDLG 30


>SPAC20G8.04c |||mitochondrial electron transfer
           flavoprotein-ubiquinone
           oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 632

 Score = 28.3 bits (60), Expect = 2.6
 Identities = 11/26 (42%), Positives = 16/26 (61%)

Query: 31  DADLVVIGSGPGGYVAAIKAAQLGMK 56
           D D+ ++G+GP G  AAI+  Q   K
Sbjct: 90  DVDVCIVGAGPAGLSAAIRIKQQAAK 115


>SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl
          modification enzyme|Schizosaccharomyces pombe|chr
          2|||Manual
          Length = 666

 Score = 27.9 bits (59), Expect = 3.4
 Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 2/47 (4%)

Query: 18 LVRIATRQYATTHDA--DLVVIGSGPGGYVAAIKAAQLGMKVVSVEK 62
          + R   R + T+ +   ++VVIG G  G  AA  A++LG K   + K
Sbjct: 1  MFRTTKRYFCTSFNRRKNVVVIGGGHAGVEAAAAASRLGAKTTLLTK 47



 Score = 27.1 bits (57), Expect = 6.0
 Identities = 12/29 (41%), Positives = 17/29 (58%)

Query: 202 KKMLVIGAGVIGLELGSVYQRLGADVTAI 230
           K ++VIG G  G+E  +   RLGA  T +
Sbjct: 17  KNVVVIGGGHAGVEAAAAASRLGAKTTLL 45


>SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate
           transaminase |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 696

 Score = 27.1 bits (57), Expect = 6.0
 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%)

Query: 347 PMLAHKAEDEGIVCVEGIKG-MPVHFNYDAIPSVIYT 382
           P L H+A+    V  EG+ G +   F  DA P + YT
Sbjct: 250 PDLLHRAQSRAFVSGEGVPGPIEYFFASDATPIIEYT 286


>SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 697

 Score = 26.6 bits (56), Expect = 7.9
 Identities = 20/86 (23%), Positives = 35/86 (40%), Gaps = 2/86 (2%)

Query: 159 VETVNTKNILIASGSEVTPFPGVTFDEKQIITSTGALSLESVPKKMLVIGAGVIGLELGS 218
           VE ++T + LI S     PFP +    + +I       ++   KK+       +G    S
Sbjct: 472 VEQIDTTHYLIGSAIGPHPFPTIVKTFQSVIGEETKAQMQEKRKKLPDAVVACVGGGSNS 531

Query: 219 V--YQRLGADVTAIEFLTSIGGVGID 242
           +  +    AD + +      GG G+D
Sbjct: 532 IGMFSPFKADKSVMMLGCEAGGDGVD 557


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.316    0.137    0.391 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,146,981
Number of Sequences: 5004
Number of extensions: 91307
Number of successful extensions: 214
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 188
Number of HSP's gapped (non-prelim): 25
length of query: 496
length of database: 2,362,478
effective HSP length: 76
effective length of query: 420
effective length of database: 1,982,174
effective search space: 832513080
effective search space used: 832513080
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 56 (26.6 bits)

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