BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000292-TA|BGIBMGA000292-PA|IPR013027|FAD-dependent pyridine nucleotide-disulphide oxidoreductase, IPR006258|Dihydrolipoamide dehydrogenase, IPR012999|Pyridine nucleotide-disulphide oxidoreductase, class I, active site, IPR001327|Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region, IPR000815|Mercuric reductase, IPR004099|Pyridine nucleotide-disulphide oxidoreductase dimerisation region, IPR001100|Pyridine nucleotide-disulphide oxidoreductase, class I (496 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase Dld1|Schi... 567 e-162 SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces pom... 160 5e-40 SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolo... 53 8e-08 SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 37 0.007 SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc... 31 0.37 SPBC3F6.03 |trr1|caf4|thioredoxin reductase Trr1|Schizosaccharom... 31 0.49 SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 30 0.85 SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1 |Schizosacch... 29 1.1 SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr ... 29 1.1 SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic enzyme|Schizosaccha... 29 2.0 SPCC1450.07c |||D-amino acid oxidase |Schizosaccharomyces pombe|... 28 2.6 SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubi... 28 2.6 SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica... 28 3.4 SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate transa... 27 6.0 SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe... 27 7.9 >SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase Dld1|Schizosaccharomyces pombe|chr 1|||Manual Length = 511 Score = 567 bits (1400), Expect = e-162 Identities = 268/479 (55%), Positives = 352/479 (73%), Gaps = 3/479 (0%) Query: 20 RIATRQYATTHDADLVVIGSGPGGYVAAIKAAQLGMKVVSVEKDPTLGGTCLNVGCIPSK 79 R+ + + + + DL VIG GPGGYVAAI+ AQLG+K + VEK TLGGTCLNVGCIPSK Sbjct: 34 RLYSAKASGNGEYDLCVIGGGPGGYVAAIRGAQLGLKTICVEKRGTLGGTCLNVGCIPSK 93 Query: 80 ALLHNSHLYHMAKHDFKQRGIETGEVTFDFKKMMEYKANAVKGLTGGIAMLFQKNKVNLV 139 ALL+NSH+YH KHD K+RGI+ V+ + +MM+ K ++VK LT GI LF+KNKV Sbjct: 94 ALLNNSHIYHTVKHDTKRRGIDVSGVSVNLSQMMKAKDDSVKSLTSGIEYLFKKNKVEYA 153 Query: 140 KGVGTIVAPNKVEVHGEKGV--ETVNTKNILIASGSEVTPFPGVTFDEKQIITSTGALSL 197 KG G+ + P + V G G +T+ KN +IA+GSEV PFPGVT DEK+I++STGALSL Sbjct: 154 KGTGSFIDPQTLSVKGIDGAADQTIKAKNFIIATGSEVKPFPGVTIDEKKIVSSTGALSL 213 Query: 198 ESVPKKMLVIGAGVIGLELGSVYQRLGADVTAIEFLTSIGGVGIDGEVAKTLQKILSKQG 257 VPKKM V+G G+IGLE+GSV+ RLGA+VT +EFL ++GG +D +++K L +I+SKQG Sbjct: 214 SEVPKKMTVLGGGIIGLEMGSVWSRLGAEVTVVEFLPAVGGP-MDADISKALSRIISKQG 272 Query: 258 MKFKLGTKVLGVKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRRPYTKGLGLDKVGIA 317 +KFK TK+L K G +++V++E K +E DV+L++IGR PYT+GLGLDK+GI+ Sbjct: 273 IKFKTSTKLLSAKVNGDSVEVEIENMKNNKRETYQTDVLLVAIGRVPYTEGLGLDKLGIS 332 Query: 318 LDDRGRIPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGIVCVEGIKGMPVHFNYDAIP 377 +D R+ ++++++T +P I IGD GPMLAHKAEDEGI VE I H NY+ IP Sbjct: 333 MDKSNRVIMDSEYRTNIPHIRVIGDATLGPMLAHKAEDEGIAAVEYIAKGQGHVNYNCIP 392 Query: 378 SVIYTSPEVGWVGKTEEDLKKEGRAYKVGKFPFLANSRAKTNGETEGFVKVLSDKTTDVI 437 +V+YT PEV WVG TE+ K+ G Y++G FPF ANSRAKTN + +G VKV+ D TD + Sbjct: 393 AVMYTHPEVAWVGITEQKAKESGIKYRIGTFPFSANSRAKTNMDADGLVKVIVDAETDRL 452 Query: 438 LGTHIIGPGGGELINEAVLAQEYGAAAEDVARVCHAHPTCAEALREANLAAYSGKPINF 496 LG H+IGP GELI EA LA EYGA+AEDVARVCHAHPT +EA +EA +AA+ GK I+F Sbjct: 453 LGVHMIGPMAGELIGEATLALEYGASAEDVARVCHAHPTLSEATKEAMMAAWCGKSIHF 511 >SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces pombe|chr 2|||Manual Length = 464 Score = 160 bits (388), Expect = 5e-40 Identities = 137/466 (29%), Positives = 216/466 (46%), Gaps = 31/466 (6%) Query: 33 DLVVIGSGPGGYVAAIKAAQLGMKVVSVEKDPTLGGTCLNVGCIPSKALLHNSHLYHMAK 92 D +VIG G GG +A +AA+ G KV +E LGGTC+N GC+P K + + + L K Sbjct: 9 DYLVIGGGSGGLASARRAAKHGAKVALIEASGRLGGTCVNYGCVPKKIMWNIADLVAKMK 68 Query: 93 HDFKQRGIETGEV-TFDFKKMMEYKANAVKGLTGGI-AMLFQKNKVNLVKGVGTIVAPNK 150 KQ G ++ +FD+ M++ K +A G GI K+ V + G + V+P + Sbjct: 69 -TAKQNGFPNSQLGSFDW-GMIKRKRDAYIGRLNGIYERNVNKDGVAYISGHASFVSPTE 126 Query: 151 --VEVHGEKGVETVNTKNILIA-SGSEVTP--FPGVTFDEKQIITSTGALSLESVPKKML 205 V+++ G + + K ILIA G + P PG + I S G LES PK++ Sbjct: 127 VAVDMNDGSGTQVFSAKYILIAVGGHPIWPSHIPGAEYG----IDSDGFFELESQPKRVA 182 Query: 206 VIGAGVIGLELGSVYQRLGAD----VTAIEFLTSIGGVGIDGEVAKTLQKILSKQGMKFK 261 ++GAG I +EL V+ LG + + +FL + DG + Q I Sbjct: 183 IVGAGYIAVELAGVFAALGTETHMFIRQSKFLRKFDPIISDG-IMDHFQHI------GIN 235 Query: 262 LGTKVLGVKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRRPYTKGLGLDKVGIALDDR 321 + T L KK ++ + + D +L +IGR P +GL L+K G+ Sbjct: 236 VHTNSLEFKKVEKLPSGEL-CIHQQDGSTFNVDTLLWAIGRAPKIQGLRLEKAGVKTLPN 294 Query: 322 GRIPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGIVCVEGIKG--MPVHFNYDAIPSV 379 G I + +T VP + ++GDV L A G + + G H +Y+ +PSV Sbjct: 295 GIIIADTYQRTNVPTVLSLGDVCGKLELTPVAIAAGRRLSDRLFGGIKDAHLDYEEVPSV 354 Query: 380 IYTSPEVGWVGKTEED-LKKEGRA-YKV--GKFPFLANSRAKTNGETEGFVKVLSDKTTD 435 ++ PE G +G TE++ + K G + KV KF L S + + K++ Sbjct: 355 VFAHPEAGTIGLTEQEAIDKYGESQIKVYNTKFNGLNYSMVEQEDKVPTTYKLVCAGPLQ 414 Query: 436 VILGTHIIGPGGGELINEAVLAQEYGAAAEDVARVCHAHPTCAEAL 481 ++G H++G E++ +A + GA D HPT AE L Sbjct: 415 KVVGLHLVGDFSAEILQGFGVAIKMGATKSDFDSCVAIHPTSAEEL 460 >SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolog Aif1|Schizosaccharomyces pombe|chr 1|||Manual Length = 575 Score = 53.2 bits (122), Expect = 8e-08 Identities = 49/198 (24%), Positives = 90/198 (45%), Gaps = 18/198 (9%) Query: 161 TVNTKNILIASGSEVTPFPGVTFDEKQII--------TSTGALSLESVPKK-MLVIGAGV 211 T + +++A+G E P D K + + A++ E+ KK +++IG+ Sbjct: 252 TESYTKLILATGGEPNKLPIPGLDSKNVYLLRSIADASKLAAVTTEAGDKKNIVIIGSSF 311 Query: 212 IGLELGSVYQRLGADVTAIEFLTSIGGVGIDGEVAKTLQKILSKQGMKFKLGTKVLGVK- 270 IGLEL V + V +E + +G EV L+ + + G+ F L + VK Sbjct: 312 IGLELAVVLKDHNVSVIGMESIPFEKVMG--KEVGTALKALHEQNGIAFYLENSIKEVKT 369 Query: 271 KEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRRPYTKGLGLDKVGIALDDRGRIPVNNKF 330 + K + K G + + DVV+++ G +P + LG ++L+ G + V+ Sbjct: 370 SSNDSSKAEHIVLKDG--QSIPADVVILAAGVKPNLRYLG---NAVSLEKDGGVKVDEHC 424 Query: 331 QTT-VPGIYAIGDVIHGP 347 + +YA+GD+ H P Sbjct: 425 RVLGAEDVYAVGDIAHAP 442 >SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2111 Score = 36.7 bits (81), Expect = 0.007 Identities = 26/83 (31%), Positives = 39/83 (46%), Gaps = 10/83 (12%) Query: 287 NKEVLDCDVVLISIG----RRPYTKGLGLDKVGIALDDRGRIPVNNK-FQTTVPGIYAIG 341 ++E D+V++++G T G+ +D+ D R I K ++T+VPGIYA G Sbjct: 2011 SEEFFPADLVILALGFLGPEEQATAGMNVDR-----DARSNISTPTKSYETSVPGIYAAG 2065 Query: 342 DVIHGPMLAHKAEDEGIVCVEGI 364 D G L EG C I Sbjct: 2066 DCRRGQSLVVWGIQEGRQCAREI 2088 Score = 27.9 bits (59), Expect = 3.4 Identities = 14/35 (40%), Positives = 19/35 (54%) Query: 34 LVVIGSGPGGYVAAIKAAQLGMKVVSVEKDPTLGG 68 + +IGSGP G AA + + G VV E+ GG Sbjct: 1758 VAIIGSGPAGLAAADQLNRAGHHVVIYERADRPGG 1792 >SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 457 Score = 31.1 bits (67), Expect = 0.37 Identities = 13/35 (37%), Positives = 21/35 (60%) Query: 29 THDADLVVIGSGPGGYVAAIKAAQLGMKVVSVEKD 63 T DAD+++IG+G G + G KV+ +E+D Sbjct: 3 TQDADIIIIGAGITGCALGAALGRQGRKVLVLERD 37 Score = 27.1 bits (57), Expect = 6.0 Identities = 11/28 (39%), Positives = 17/28 (60%) Query: 204 MLVIGAGVIGLELGSVYQRLGADVTAIE 231 +++IGAG+ G LG+ R G V +E Sbjct: 8 IIIIGAGITGCALGAALGRQGRKVLVLE 35 >SPBC3F6.03 |trr1|caf4|thioredoxin reductase Trr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 322 Score = 30.7 bits (66), Expect = 0.49 Identities = 23/58 (39%), Positives = 31/58 (53%), Gaps = 6/58 (10%) Query: 289 EVLDCDV--VLISIGRRPYTKGLGLDKVGIALDDRGRIP-VNNKFQTTVPGIYAIGDV 343 EV D V + +IG P TK L I LD+ G I +N +T++PG +A GDV Sbjct: 235 EVSDLQVNGLFYAIGHIPATK---LVAEQIELDEAGYIKTINGTPRTSIPGFFAAGDV 289 Score = 30.3 bits (65), Expect = 0.64 Identities = 16/33 (48%), Positives = 22/33 (66%), Gaps = 1/33 (3%) Query: 29 THDADLVVIGSGPGGYVAAIKAAQLGMKVVSVE 61 TH+ +V+IGSGP G+ AAI A+ +K V E Sbjct: 2 THNK-VVIIGSGPAGHTAAIYLARGELKPVMYE 33 >SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual Length = 1279 Score = 29.9 bits (64), Expect = 0.85 Identities = 26/78 (33%), Positives = 36/78 (46%), Gaps = 11/78 (14%) Query: 374 DAIPSVIY-TSPE----VGWVGKTEEDLKKEGR-AYKVGKFPFLANSRAKTNGETEGFVK 427 DA P+++ T P V WV KT + G + K PF A+SRA + T Sbjct: 85 DAFPTLLSKTGPSKPRIVSWVRKTASNTSVAGSDSVSRDKIPFSASSRASSTKST----- 139 Query: 428 VLSDKTTDVILGTHIIGP 445 + S K TD + T I+ P Sbjct: 140 LSSVKETDFVTETLILSP 157 >SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 469 Score = 29.5 bits (63), Expect = 1.1 Identities = 17/39 (43%), Positives = 20/39 (51%), Gaps = 1/39 (2%) Query: 310 GLDKVGIALDDRGRIPVNNKFQTTVPGIYAIGDVIHGPM 348 G+ VG+ DD I V N PGIY G V HGP+ Sbjct: 344 GMKDVGVPFDDAKGI-VKNVNGFVRPGIYTSGWVKHGPI 381 >SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 29.5 bits (63), Expect = 1.1 Identities = 16/58 (27%), Positives = 25/58 (43%) Query: 11 PTFRSGSLVRIATRQYATTHDADLVVIGSGPGGYVAAIKAAQLGMKVVSVEKDPTLGG 68 P+ S S +T ++ +VIG G G A LG V+ ++K+ GG Sbjct: 18 PSTNSASFFSTLRFNMSTANNTQAIVIGGGLAGLSATNTILDLGGNVLLLDKNTAFGG 75 >SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic enzyme|Schizosaccharomyces pombe|chr 2|||Manual Length = 328 Score = 28.7 bits (61), Expect = 2.0 Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 6/61 (9%) Query: 14 RSGSLVRIATRQYATTHD----ADLVVIGSGPGGYVAA--IKAAQLGMKVVSVEKDPTLG 67 R ++ R TR+Y + D +D+V++G+G G AA I + +K+ +E G Sbjct: 55 RESTVSRAMTRRYFSDLDKYAESDIVIVGAGSAGLTAAYYIGTRRPDLKIAIIEASVAPG 114 Query: 68 G 68 G Sbjct: 115 G 115 >SPCC1450.07c |||D-amino acid oxidase |Schizosaccharomyces pombe|chr 3|||Manual Length = 348 Score = 28.3 bits (60), Expect = 2.6 Identities = 11/30 (36%), Positives = 20/30 (66%) Query: 195 LSLESVPKKMLVIGAGVIGLELGSVYQRLG 224 ++ E+ P+ ++++GAGVIGL + LG Sbjct: 1 MTKENKPRDIVIVGAGVIGLTTAWILSDLG 30 >SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubiquinone oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 28.3 bits (60), Expect = 2.6 Identities = 11/26 (42%), Positives = 16/26 (61%) Query: 31 DADLVVIGSGPGGYVAAIKAAQLGMK 56 D D+ ++G+GP G AAI+ Q K Sbjct: 90 DVDVCIVGAGPAGLSAAIRIKQQAAK 115 >SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modification enzyme|Schizosaccharomyces pombe|chr 2|||Manual Length = 666 Score = 27.9 bits (59), Expect = 3.4 Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 2/47 (4%) Query: 18 LVRIATRQYATTHDA--DLVVIGSGPGGYVAAIKAAQLGMKVVSVEK 62 + R R + T+ + ++VVIG G G AA A++LG K + K Sbjct: 1 MFRTTKRYFCTSFNRRKNVVVIGGGHAGVEAAAAASRLGAKTTLLTK 47 Score = 27.1 bits (57), Expect = 6.0 Identities = 12/29 (41%), Positives = 17/29 (58%) Query: 202 KKMLVIGAGVIGLELGSVYQRLGADVTAI 230 K ++VIG G G+E + RLGA T + Sbjct: 17 KNVVVIGGGHAGVEAAAAASRLGAKTTLL 45 >SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate transaminase |Schizosaccharomyces pombe|chr 2|||Manual Length = 696 Score = 27.1 bits (57), Expect = 6.0 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%) Query: 347 PMLAHKAEDEGIVCVEGIKG-MPVHFNYDAIPSVIYT 382 P L H+A+ V EG+ G + F DA P + YT Sbjct: 250 PDLLHRAQSRAFVSGEGVPGPIEYFFASDATPIIEYT 286 >SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe|chr 1|||Manual Length = 697 Score = 26.6 bits (56), Expect = 7.9 Identities = 20/86 (23%), Positives = 35/86 (40%), Gaps = 2/86 (2%) Query: 159 VETVNTKNILIASGSEVTPFPGVTFDEKQIITSTGALSLESVPKKMLVIGAGVIGLELGS 218 VE ++T + LI S PFP + + +I ++ KK+ +G S Sbjct: 472 VEQIDTTHYLIGSAIGPHPFPTIVKTFQSVIGEETKAQMQEKRKKLPDAVVACVGGGSNS 531 Query: 219 V--YQRLGADVTAIEFLTSIGGVGID 242 + + AD + + GG G+D Sbjct: 532 IGMFSPFKADKSVMMLGCEAGGDGVD 557 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.316 0.137 0.391 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,146,981 Number of Sequences: 5004 Number of extensions: 91307 Number of successful extensions: 214 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 188 Number of HSP's gapped (non-prelim): 25 length of query: 496 length of database: 2,362,478 effective HSP length: 76 effective length of query: 420 effective length of database: 1,982,174 effective search space: 832513080 effective search space used: 832513080 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 56 (26.6 bits)
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