BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000292-TA|BGIBMGA000292-PA|IPR013027|FAD-dependent pyridine nucleotide-disulphide oxidoreductase, IPR006258|Dihydrolipoamide dehydrogenase, IPR012999|Pyridine nucleotide-disulphide oxidoreductase, class I, active site, IPR001327|Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region, IPR000815|Mercuric reductase, IPR004099|Pyridine nucleotide-disulphide oxidoreductase dimerisation region, IPR001100|Pyridine nucleotide-disulphide oxidoreductase, class I (496 letters) Database: mosquito 2123 sequences; 516,269 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AJ549084-1|CAD70158.1| 505|Anopheles gambiae thioredoxin-disulf... 152 2e-38 AJ549085-1|CAD70159.1| 529|Anopheles gambiae thioredoxin-disulf... 151 3e-38 AJ459821-1|CAD30858.1| 502|Anopheles gambiae thioredoxin reduct... 151 4e-38 AY341177-1|AAR13741.1| 230|Anopheles gambiae ferredoxin reducta... 40 2e-04 AY341178-1|AAR13742.1| 230|Anopheles gambiae ferredoxin reducta... 39 3e-04 AY341176-1|AAR13740.1| 230|Anopheles gambiae ferredoxin reducta... 39 3e-04 AY341175-1|AAR13739.1| 230|Anopheles gambiae ferredoxin reducta... 39 3e-04 AF395080-1|AAK97462.1| 537|Anopheles gambiae zinc finger transc... 27 1.2 M93690-2|AAA29363.1| 1212|Anopheles gambiae unknown protein. 24 8.1 >AJ549084-1|CAD70158.1| 505|Anopheles gambiae thioredoxin-disulfide reductase protein. Length = 505 Score = 152 bits (369), Expect = 2e-38 Identities = 146/500 (29%), Positives = 226/500 (45%), Gaps = 38/500 (7%) Query: 22 ATRQYATTHDADLVVIGSGPGGYVAAIKAAQLGMKVVS---VEKDPT-----LGGTCLNV 73 AT ++ DLVVIG G GG A +A QLG KV V+ P LGGTC+NV Sbjct: 4 ATAAEQENYEYDLVVIGGGSGGLACAKQAVQLGAKVAVLDFVKPSPRGTKWGLGGTCVNV 63 Query: 74 GCIPSKALLHNSHLYHMAKHDFKQRGIETGE---VTFDFKKMMEYKANAVKGLTGGIAML 130 GCIP K L+H + L A HD + G + + + D+ + E N +K + + Sbjct: 64 GCIPKK-LMHQASLLGEAIHDSQPYGWQLPDPAAIRHDWATLTESVQNHIKSVNWVTRVD 122 Query: 131 FQKNKVNLVKGVGTIVAPNKVEVHGEKGVET-VNTKNILIASGSEVTPFPGVTFDEKQII 189 + KV V G+G + V + E + K+++IA G +P + + I Sbjct: 123 LRDQKVEYVNGLGYFKDDHTVVAVMKNQTERELRAKHVVIAVGGRPR-YPDIPGAAEYGI 181 Query: 190 TSTGALSLESVPKKMLVIGAGVIGLELGSVYQRLGADVTAIEFLTSIGGVGIDGEVAKTL 249 TS SL P + L++GAG IGLE + LG DV+ + + SI G D ++A + Sbjct: 182 TSDDIFSLPQAPGRTLLVGAGYIGLECAGFLKGLGYDVSVM--VRSILLRGFDQQMATMV 239 Query: 250 QKILSKQGMKFKLGTKVLGVKKEGS---TIKVDV--EAAKGGNKEVLDCDVVLISIGRRP 304 + ++G++F ++ L V+K+ ++ + EA N E + D VL +IGR+ Sbjct: 240 GDSMVEKGIRFHHRSRPLAVEKQPDGRLLVRYETVDEAGTATNGEDV-FDTVLFAIGRQA 298 Query: 305 YTKGLGLDKVGIALDDRG---RIPVN-NKFQTTVPGIYAIGDVIH-GPMLAHKAEDEG-I 358 T L L G+ + G ++ V+ +T VP IYA+GDV++ P L A G I Sbjct: 299 ETGTLKLANAGVVTAEGGKSDKLEVDETDHRTNVPHIYAVGDVLYRKPELTPVAIHAGRI 358 Query: 359 VCVEGIKGMPVHFNYDAIPSVIYTSPEVGWVGKTEEDLK----KEG-RAYKVGKFP--FL 411 + G +Y + + ++T E G VG +EE + K+G Y P F Sbjct: 359 IARRLFGGSEERMDYADVATTVFTPLEYGCVGLSEEAAEAAHGKDGIEVYHAYYKPTEFF 418 Query: 412 ANSRAKTNGETEGFVKVLSDKTTDVILGTHIIGPGGGELINEAVLAQEYGAAAEDVARVC 471 R+ + V + +LG H +GP GE+I A + G + + Sbjct: 419 VPQRSVRYCYLKA---VALREGNQRVLGLHFLGPAAGEVIQGFAAALKCGLTMQVLRNTV 475 Query: 472 HAHPTCAEALREANLAAYSG 491 HPT AE + SG Sbjct: 476 GIHPTVAEEFTRLAITKRSG 495 >AJ549085-1|CAD70159.1| 529|Anopheles gambiae thioredoxin-disulfide reductase protein. Length = 529 Score = 151 bits (367), Expect = 3e-38 Identities = 147/501 (29%), Positives = 228/501 (45%), Gaps = 39/501 (7%) Query: 22 ATRQYAT-THDADLVVIGSGPGGYVAAIKAAQLGMKVVS---VEKDPT-----LGGTCLN 72 AT +A ++ DLVVIG G GG A +A QLG KV V+ P LGGTC+N Sbjct: 27 ATVMFAKENYEYDLVVIGGGSGGLACAKQAVQLGAKVAVLDFVKPSPRGTKWGLGGTCVN 86 Query: 73 VGCIPSKALLHNSHLYHMAKHDFKQRGIETGE---VTFDFKKMMEYKANAVKGLTGGIAM 129 VGCIP K L+H + L A HD + G + + + D+ + E N +K + + Sbjct: 87 VGCIPKK-LMHQASLLGEAIHDSQPYGWQLPDPAAIRHDWATLTESVQNHIKSVNWVTRV 145 Query: 130 LFQKNKVNLVKGVGTIVAPNKVEVHGEKGVET-VNTKNILIASGSEVTPFPGVTFDEKQI 188 + KV V G+G + V + E + K+++IA G +P + + Sbjct: 146 DLRDQKVEYVNGLGYFKDDHTVVAVMKNQTERELRAKHVVIAVGGRPR-YPDIPGAAEYG 204 Query: 189 ITSTGALSLESVPKKMLVIGAGVIGLELGSVYQRLGADVTAIEFLTSIGGVGIDGEVAKT 248 ITS SL P + L++GAG IGLE + LG DV+ + + SI G D ++A Sbjct: 205 ITSDDIFSLPQAPGRTLLVGAGYIGLECAGFLKGLGYDVSVM--VRSILLRGFDQQMATM 262 Query: 249 LQKILSKQGMKFKLGTKVLGVKKEGS---TIKVDV--EAAKGGNKEVLDCDVVLISIGRR 303 + + ++G++F ++ L V+K+ ++ + EA N E + D VL +IGR+ Sbjct: 263 VGDSMVEKGIRFHHRSRPLAVEKQPDGRLLVRYETVDEAGTATNGEDV-FDTVLFAIGRQ 321 Query: 304 PYTKGLGLDKVGIALDDRG---RIPVN-NKFQTTVPGIYAIGDVIH-GPMLAHKAEDEG- 357 T L L G+ + G ++ V+ +T VP IYA+GDV++ P L A G Sbjct: 322 AETGTLKLANAGVVTAEGGKSDKLEVDETDHRTNVPHIYAVGDVLYRKPELTPVAIHAGR 381 Query: 358 IVCVEGIKGMPVHFNYDAIPSVIYTSPEVGWVGKTEEDLK----KEG-RAYKVGKFP--F 410 I+ G +Y + + ++T E G VG +EE + K+G Y P F Sbjct: 382 IIARRLFGGSEERMDYADVATTVFTPLEYGCVGLSEEAAEAAHGKDGIEVYHAYYKPTEF 441 Query: 411 LANSRAKTNGETEGFVKVLSDKTTDVILGTHIIGPGGGELINEAVLAQEYGAAAEDVARV 470 R+ + V + +LG H +GP GE+I A + G + + Sbjct: 442 FVPQRSVRYCYLKA---VALREGNQRVLGLHFLGPAAGEVIQGFAAALKCGLTMQVLRNT 498 Query: 471 CHAHPTCAEALREANLAAYSG 491 HPT AE + SG Sbjct: 499 VGIHPTVAEEFTRLAITKRSG 519 >AJ459821-1|CAD30858.1| 502|Anopheles gambiae thioredoxin reductase protein. Length = 502 Score = 151 bits (366), Expect = 4e-38 Identities = 144/492 (29%), Positives = 224/492 (45%), Gaps = 38/492 (7%) Query: 30 HDADLVVIGSGPGGYVAAIKAAQLGMKVVS---VEKDPT-----LGGTCLNVGCIPSKAL 81 ++ DLVVIG G GG A +A QLG KV V+ P LGGTC+NVGCIP K L Sbjct: 9 YEYDLVVIGGGSGGLACAKQAVQLGAKVAVLDFVKPSPRGTKWGLGGTCVNVGCIPKK-L 67 Query: 82 LHNSHLYHMAKHDFKQRGIETGE---VTFDFKKMMEYKANAVKGLTGGIAMLFQKNKVNL 138 +H + L A HD + G + + + D+ + E N +K + + + KV Sbjct: 68 MHQASLLGEAIHDSQPYGWQLPDPAAIRHDWATLTESVQNHIKSVNWVTRVDLRDQKVEY 127 Query: 139 VKGVGTIVAPNKVEVHGEKGVET-VNTKNILIASGSEVTPFPGVTFDEKQIITSTGALSL 197 V G+G + V + E + K+++IA G +P + + ITS SL Sbjct: 128 VNGLGYFKDDHTVVAVMKNQTERELRAKHVVIAVGGRPR-YPDIPGAAEYGITSDDIFSL 186 Query: 198 ESVPKKMLVIGAGVIGLELGSVYQRLGADVTAIEFLTSIGGVGIDGEVAKTLQKILSKQG 257 P + L++GAG IGLE + LG DV+ + + SI G D ++A + + ++G Sbjct: 187 PQAPGRTLLVGAGYIGLECAGFLKGLGYDVSVM--VRSILLRGFDQQMATMVGDSMVEKG 244 Query: 258 MKFKLGTKVLGVKKEGS---TIKVDV--EAAKGGNKEVLDCDVVLISIGRRPYTKGLGLD 312 ++F ++ L V+K+ ++ + EA N E + D VL +IGR+ T L L Sbjct: 245 IRFHHRSRPLAVEKQPDGRLLVRYETVDEAGTATNGEDV-FDTVLFAIGRQAETGTLKLA 303 Query: 313 KVGIALDDRG---RIPVN-NKFQTTVPGIYAIGDVIH-GPMLAHKAEDEG-IVCVEGIKG 366 G+ + G ++ V+ +T VP IYA+GDV++ P L A G I+ G Sbjct: 304 NAGVVTAEGGKSDKLEVDETDHRTNVPHIYAVGDVLYRKPELTPVAIHAGRIIARRLFGG 363 Query: 367 MPVHFNYDAIPSVIYTSPEVGWVGKTEEDLK----KEG-RAYKVGKFP--FLANSRAKTN 419 +Y + + ++T E G VG +EE + K+G Y P F R+ Sbjct: 364 SEERMDYADVATTVFTPLEYGCVGLSEEAAEAAHGKDGIEVYHAYYKPTEFFVPQRSVRY 423 Query: 420 GETEGFVKVLSDKTTDVILGTHIIGPGGGELINEAVLAQEYGAAAEDVARVCHAHPTCAE 479 + V + +LG H +GP GE+I A + G + + HPT AE Sbjct: 424 CYLKA---VALREGNQRVLGLHFLGPAAGEVIQGFAAALKCGLTMQVLRNTVGIHPTVAE 480 Query: 480 ALREANLAAYSG 491 + SG Sbjct: 481 EFTRLAITKRSG 492 Score = 25.0 bits (52), Expect = 4.7 Identities = 13/39 (33%), Positives = 21/39 (53%) Query: 195 LSLESVPKKMLVIGAGVIGLELGSVYQRLGADVTAIEFL 233 L+ E+ ++VIG G GL +LGA V ++F+ Sbjct: 4 LNQENYEYDLVVIGGGSGGLACAKQAVQLGAKVAVLDFV 42 >AY341177-1|AAR13741.1| 230|Anopheles gambiae ferredoxin reductase protein. Length = 230 Score = 39.9 bits (89), Expect = 2e-04 Identities = 25/114 (21%), Positives = 56/114 (49%), Gaps = 6/114 (5%) Query: 245 VAKTLQKILSKQGMKFKLGTKVLGVKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRRP 304 + + + ++ ++G++F + + + T++ VE G +LD D+ + IG Sbjct: 11 IGQRVMELFEEKGVQFVMNSGIRRCIGAEGTVQ-QVELTDG---TLLDADICIFGIGSTL 66 Query: 305 YTKGLGLDKVGIALDDRGRIPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGI 358 YT+ L GI+L+ G I + ++ + G+Y GD+ + P+ ++ + I Sbjct: 67 YTEFL--QGSGISLNRNGSINTDQYLESNLEGVYVGGDIANAPVHSNDGQQATI 118 >AY341178-1|AAR13742.1| 230|Anopheles gambiae ferredoxin reductase protein. Length = 230 Score = 39.1 bits (87), Expect = 3e-04 Identities = 24/115 (20%), Positives = 59/115 (51%), Gaps = 8/115 (6%) Query: 245 VAKTLQKILSKQGMKFKLGTKVLG-VKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRR 303 + + + ++ ++G++F + + + + EG+ +V++ + +LD D+ + IG Sbjct: 11 IGQRVMELFEEKGVQFVMNSGIRRCIGAEGAVQQVELT-----DGTLLDADICIFGIGST 65 Query: 304 PYTKGLGLDKVGIALDDRGRIPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGI 358 YT+ L GI+L+ G I + ++ + G+Y GD+ + P+ ++ + I Sbjct: 66 LYTEFL--QGSGISLNRNGSINTDQYLESNLEGVYVGGDIANAPVHSNDGQQATI 118 >AY341176-1|AAR13740.1| 230|Anopheles gambiae ferredoxin reductase protein. Length = 230 Score = 39.1 bits (87), Expect = 3e-04 Identities = 24/115 (20%), Positives = 59/115 (51%), Gaps = 8/115 (6%) Query: 245 VAKTLQKILSKQGMKFKLGTKVLG-VKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRR 303 + + + ++ ++G++F + + + + EG+ +V++ + +LD D+ + IG Sbjct: 11 IGQRVMELFEEKGVQFVMNSGIRRCIGAEGAVQQVELT-----DGTLLDADICIFGIGST 65 Query: 304 PYTKGLGLDKVGIALDDRGRIPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGI 358 YT+ L GI+L+ G I + ++ + G+Y GD+ + P+ ++ + I Sbjct: 66 LYTEFL--QGSGISLNRNGSINTDQYLESNLEGVYVGGDIANAPVHSNDGQQATI 118 >AY341175-1|AAR13739.1| 230|Anopheles gambiae ferredoxin reductase protein. Length = 230 Score = 39.1 bits (87), Expect = 3e-04 Identities = 24/115 (20%), Positives = 59/115 (51%), Gaps = 8/115 (6%) Query: 245 VAKTLQKILSKQGMKFKLGTKVLG-VKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRR 303 + + + ++ ++G++F + + + + EG+ +V++ + +LD D+ + IG Sbjct: 11 IGQRVMELFEEKGVQFVMNSGIRRCIGAEGAVQQVELT-----DGTLLDADICIFGIGST 65 Query: 304 PYTKGLGLDKVGIALDDRGRIPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGI 358 YT+ L GI+L+ G I + ++ + G+Y GD+ + P+ ++ + I Sbjct: 66 LYTEFL--QGSGISLNRNGSINTDQYLESNLEGVYVGGDIANAPVHSNDGQQATI 118 >AF395080-1|AAK97462.1| 537|Anopheles gambiae zinc finger transcription factor pannier protein. Length = 537 Score = 27.1 bits (57), Expect = 1.2 Identities = 13/39 (33%), Positives = 17/39 (43%) Query: 441 HIIGPGGGELINEAVLAQEYGAAAEDVARVCHAHPTCAE 479 H + PG G +N A L + A H HPT A+ Sbjct: 479 HHVSPGMGSTVNGASLTHSHHAHPHHHHHHHHHHPTAAD 517 >M93690-2|AAA29363.1| 1212|Anopheles gambiae unknown protein. Length = 1212 Score = 24.2 bits (50), Expect = 8.1 Identities = 23/78 (29%), Positives = 35/78 (44%), Gaps = 6/78 (7%) Query: 265 KVLGVKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRRPYTKG--LGLD-KVGIALDDR 321 KVL V G I D+ EV DV+++S RP +D +A+ Sbjct: 62 KVLQVNLGGGRIAQDLVLQTARQMEV---DVLVLSHTYRPPENNPRWAVDASKKVAVVAT 118 Query: 322 GRIPVNNKFQTTVPGIYA 339 GR P+ ++ + VPG+ A Sbjct: 119 GRYPLQGQWSSDVPGLIA 136 Database: mosquito Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 516,269 Number of sequences in database: 2123 Lambda K H 0.316 0.137 0.391 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 485,512 Number of Sequences: 2123 Number of extensions: 20349 Number of successful extensions: 56 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 26 Number of HSP's gapped (non-prelim): 17 length of query: 496 length of database: 516,269 effective HSP length: 67 effective length of query: 429 effective length of database: 374,028 effective search space: 160458012 effective search space used: 160458012 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 50 (24.2 bits)
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