Silkworm BLAST Result against nematostella

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000291-TA|BGIBMGA000291-PA|IPR002076|GNS1/SUR4 membrane
protein
         (283 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_7377| Best HMM Match : ELO (HMM E-Value=3.6e-06)                   138   4e-33
SB_7376| Best HMM Match : ELO (HMM E-Value=3.3e-07)                    93   2e-19
SB_43724| Best HMM Match : AMP-binding (HMM E-Value=2.6e-09)           33   0.20 
SB_13704| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   2.5  
SB_1228| Best HMM Match : PKD_channel (HMM E-Value=0)                  29   5.7  
SB_51012| Best HMM Match : 7tm_1 (HMM E-Value=2e-06)                   28   7.5  
SB_16221| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.5  
SB_51114| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   10.0 

>SB_7377| Best HMM Match : ELO (HMM E-Value=3.6e-06)
          Length = 255

 Score =  138 bits (335), Expect = 4e-33
 Identities = 82/234 (35%), Positives = 123/234 (52%), Gaps = 31/234 (13%)

Query: 19  SDQRVKDWFLMSSPFPTLAICLTYVFIVKVLGPKLMENRKPFELKQVLIWYNLFQVIFSC 78
           SD R K+W  ++     + +   Y+  V + GP LM+N+K F L+ +++ YN    + + 
Sbjct: 22  SDPRTKNWPFVNDVRVPIGLLFAYILAV-LTGPHLMKNKKAFNLQWIMVIYNGMMSLLNL 80

Query: 79  WLFYETVNGCWWYYXXXXXXXXXXXXXVMRKKFDHVSKLHVIHHGIMPMSVWFGVKFTPG 138
           ++F E                      ++RKK + +S LHV HH  M    W G+K+  G
Sbjct: 81  YIFLEMF-------------------FILRKKNNQISFLHVYHHFSMVCLWWVGIKWVAG 121

Query: 139 GHSTFFGMLNTFVHIVMYSYYLLAALGPQVQKYLWWKKYLTALQMVQFVLVFIHAFQLLF 198
           G S F  M+N F+HI+MY+YY L+A+ P+++ YLWWKKYLT LQ+ QF  V  H    L+
Sbjct: 122 GQSFFSAMINAFIHIIMYAYYGLSAI-PEMRIYLWWKKYLTQLQLTQFCTVMAHGVLSLW 180

Query: 199 IDCDYPRAFVWW----IGMHAVLFYYLFSDFYKQAYLRKSKKA--KSMMKVEDE 246
             C   R F+W     IG + + F  LFS+FY  AYL + +    K  M+   E
Sbjct: 181 AKC---RFFLWMQYALIG-YMISFLILFSNFYLHAYLTRKQSCPNKDAMQANGE 230


>SB_7376| Best HMM Match : ELO (HMM E-Value=3.3e-07)
          Length = 278

 Score = 93.1 bits (221), Expect = 2e-19
 Identities = 41/98 (41%), Positives = 63/98 (64%), Gaps = 2/98 (2%)

Query: 141 STFFGML-NTFVHIVMYSYYLLAALGPQVQKYLWWKKYLTALQMVQFV-LVFIHAFQLLF 198
           + +FG   N+F+H++MY YY L+A+GP VQKYLWWK+YLT +Q++QF+ L+++  + L  
Sbjct: 179 TAYFGAACNSFIHVIMYLYYGLSAMGPSVQKYLWWKRYLTKMQLIQFLALIYMAVYALWH 238

Query: 199 IDCDYPRAFVWWIGMHAVLFYYLFSDFYKQAYLRKSKK 236
             C   + F W    + +    LFS+FY   YL+K  K
Sbjct: 239 DGCGTHKFFQWVQFGYGMSLLVLFSNFYLHTYLKKPSK 276



 Score = 71.3 bits (167), Expect = 8e-13
 Identities = 35/88 (39%), Positives = 56/88 (63%), Gaps = 2/88 (2%)

Query: 1  MEALSRLVAGYHDLMDNKSDQRVKDWFLMSSPFPTLAICLTYVFIVKVLGPKLMENRKPF 60
          M +  RL A  + ++ +  D R+ +W L+++P+P L++   Y+FIVKV GPK MENRK +
Sbjct: 1  MTSTQRL-ADVYQIVTSVGDPRLHEWPLIATPWPCLSLMAMYLFIVKV-GPKFMENRKAW 58

Query: 61 ELKQVLIWYNLFQVIFSCWLFYETVNGC 88
          +L+ VL+ YN   V+ S ++ YE    C
Sbjct: 59 DLRGVLVVYNFALVLLSAYMVYEQSTKC 86


>SB_43724| Best HMM Match : AMP-binding (HMM E-Value=2.6e-09)
          Length = 490

 Score = 33.5 bits (73), Expect = 0.20
 Identities = 16/58 (27%), Positives = 33/58 (56%), Gaps = 2/58 (3%)

Query: 150 FVHIVMYSYYLLAALGPQVQKYLWWKKYLTALQMVQFVLVFIHAFQLL--FIDCDYPR 205
           F+ +++Y+ YL    G + ++ L +K+YL   +  +++  FIH   LL  ++D   P+
Sbjct: 425 FIEVLLYNIYLHGPEGVRYKEALIYKRYLPCPEGARYLDDFIHVVMLLRTYVDLSCPQ 482


>SB_13704| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 132

 Score = 29.9 bits (64), Expect = 2.5
 Identities = 15/52 (28%), Positives = 23/52 (44%)

Query: 201 CDYPRAFVWWIGMHAVLFYYLFSDFYKQAYLRKSKKAKSMMKVEDEPVKYET 252
           C Y   FV W    + +F  +  D+YK   L +  K K   K   E + Y++
Sbjct: 12  CGYKEFFVTWDKQESAMFVCIVMDYYKFGDLDRCLKQKRKQKEVIEEMIYKS 63


>SB_1228| Best HMM Match : PKD_channel (HMM E-Value=0)
          Length = 1157

 Score = 28.7 bits (61), Expect = 5.7
 Identities = 24/101 (23%), Positives = 45/101 (44%), Gaps = 10/101 (9%)

Query: 158 YYLLAALGPQVQKYLWWKKYLTALQMVQFVLVF----IHAFQLLFIDCDYPRAFVWWIGM 213
           Y+ +  L       L ++K+  A+Q++  +L+F    I   ++L + C Y  +F  W+ M
Sbjct: 717 YHNIQTLSLYASPNLNFRKFFNAIQIIILLLIFCYLIIEVGKVLSLKCKYFVSFWNWLNM 776

Query: 214 ----HAVLFYYLFSDFYKQAYLRKSKKAKSMMKVEDEPVKY 250
                A+    LF  F+K+ Y+ +  K       E   + Y
Sbjct: 777 LQLLTAIATVVLF--FFKEDYVSQFVKNVQRNPYETTSIDY 815


>SB_51012| Best HMM Match : 7tm_1 (HMM E-Value=2e-06)
          Length = 457

 Score = 28.3 bits (60), Expect = 7.5
 Identities = 13/52 (25%), Positives = 30/52 (57%), Gaps = 1/52 (1%)

Query: 15  MDNKSDQRVKDWFLMSSPFPTLAICLTYVFIVKVLGPKLMENRKPFELKQVL 66
           +D K+D   K  F+++S   ++AI ++YV ++ +   + +   +P   KQ++
Sbjct: 106 LDKKTDLS-KALFIIASNISSIAISVSYVIVLAMSASQYIAITRPHRYKQII 156


>SB_16221| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 834

 Score = 28.3 bits (60), Expect = 7.5
 Identities = 15/40 (37%), Positives = 20/40 (50%), Gaps = 1/40 (2%)

Query: 112 DHVSKLHVIHHGIMPMSVWFGVKFTPGGHSTFFGMLNTFV 151
           D+  KLHV+H  + P +V F  K       T FG  N F+
Sbjct: 29  DYCHKLHVVHRDLKPENVIF-FKNQDMAKLTDFGFSNNFI 67


>SB_51114| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 349

 Score = 27.9 bits (59), Expect = 10.0
 Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 1/38 (2%)

Query: 213 MHAVLFYYLFSDFYKQAYLRK-SKKAKSMMKVEDEPVK 249
           +HA+L +Y+  D Y+   +   + KAK +   E+E VK
Sbjct: 289 LHAILSHYMSEDVYRPVKITPYNTKAKEVRFTEEETVK 326


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.330    0.141    0.469 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,656,098
Number of Sequences: 59808
Number of extensions: 318589
Number of successful extensions: 1360
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1350
Number of HSP's gapped (non-prelim): 10
length of query: 283
length of database: 16,821,457
effective HSP length: 81
effective length of query: 202
effective length of database: 11,977,009
effective search space: 2419355818
effective search space used: 2419355818
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.8 bits)
S2: 59 (27.9 bits)