BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000291-TA|BGIBMGA000291-PA|IPR002076|GNS1/SUR4 membrane
protein
(283 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g06460.1 68416.m00748 GNS1/SUR4 membrane family protein simil... 52 3e-07
At3g06470.1 68416.m00749 GNS1/SUR4 membrane family protein simil... 51 8e-07
At4g31730.1 68417.m04504 expressed protein 35 0.071
At1g10760.1 68414.m01231 starch excess protein (SEX1) identical ... 29 3.5
At2g25310.1 68415.m03028 expressed protein 29 4.7
At1g15520.1 68414.m01867 ABC transporter family protein similar ... 28 8.2
>At3g06460.1 68416.m00748 GNS1/SUR4 membrane family protein similar
to SP|P25358 Elongation of fatty acids protein 2 (GNS1
protein) (V-SNARE bypass mutant gene 2 protein)
{Saccharomyces cerevisiae}; contains Pfam profile
PF01151: GNS1/SUR4 family
Length = 298
Score = 52.4 bits (120), Expect = 3e-07
Identities = 27/81 (33%), Positives = 43/81 (53%), Gaps = 5/81 (6%)
Query: 106 VMRKKFDHVSKLHVIHHGIMPMSVWFGVKFTPGGHSTFFGMLNTFVHIVMYSYYLLAALG 165
++ K +S LHV HH + + + ++ +LN+ VH++MY YY L A+G
Sbjct: 139 ILNKSIQRLSFLHVYHHATVVILCYLWLRTRQSMFPVGL-VLNSTVHVIMYGYYFLCAIG 197
Query: 166 PQVQKYLWWKKYLTALQMVQF 186
+ + WKK +T QMVQF
Sbjct: 198 SRPK----WKKLVTNFQMVQF 214
>At3g06470.1 68416.m00749 GNS1/SUR4 membrane family protein similar
to SP|P39540 Elongation of fatty acids protein 1
{Saccharomyces cerevisiae}; contains Pfam profile
PF01151: GNS1/SUR4 family
Length = 278
Score = 51.2 bits (117), Expect = 8e-07
Identities = 39/133 (29%), Positives = 67/133 (50%), Gaps = 11/133 (8%)
Query: 106 VMRKKFDHVSKLHVIHHGIMPMSVWFGVKFTPGGHSTFFGMLNTFVHIVMYSYYLLAALG 165
++ K +S LHV HH + + + ++ T + N+ VH++MY YY L A+G
Sbjct: 142 ILGKSIQRLSFLHVYHHATVVVMCYLWLR-TRQSMFPIALVTNSTVHVIMYGYYFLCAVG 200
Query: 166 PQVQKYLWWKKYLTALQMVQFVLVF-IHAFQL---LF-IDCDYPRAFVWWIGMHAVLFYY 220
+ + WK+ +T Q+VQFV F + + L LF C + + +A L
Sbjct: 201 SRPK----WKRLVTDCQIVQFVFSFGLSGWMLREHLFGSGCTGIWGWCFNAAFNASLL-A 255
Query: 221 LFSDFYKQAYLRK 233
LFS+F+ + Y++K
Sbjct: 256 LFSNFHSKNYVKK 268
>At4g31730.1 68417.m04504 expressed protein
Length = 158
Score = 34.7 bits (76), Expect = 0.071
Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 2/63 (3%)
Query: 107 MRKKFDHVS-KLHVIHHGIMPMSVWFG-VKFTPGGHSTFFGMLNTFVHIVMYSYYLLAAL 164
++ KF+ V+ V HHG+ P S W V + GG + G++ + I+ SY+ L++
Sbjct: 6 VQSKFEDVATSTSVNHHGVTPQSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWRLSSS 65
Query: 165 GPQ 167
G +
Sbjct: 66 GEE 68
>At1g10760.1 68414.m01231 starch excess protein (SEX1) identical to
SEX1 [Arabidopsis thaliana] GI:12044358; supporting cDNA
gi|12044357|gb|AF312027.1|AF312027
Length = 1399
Score = 29.1 bits (62), Expect = 3.5
Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 2/49 (4%)
Query: 228 QAYLRKSKKAKSMMKVEDEPVKYETKEMKLPLLNGFSNGSALGDVRQRM 276
QAYLR +K K M E E +YE + L G+++ D+R ++
Sbjct: 219 QAYLRWERKGKQMYNPEKEKEEYEA--ARTELREEMMRGASVEDLRAKL 265
>At2g25310.1 68415.m03028 expressed protein
Length = 210
Score = 28.7 bits (61), Expect = 4.7
Identities = 14/37 (37%), Positives = 22/37 (59%)
Query: 28 LMSSPFPTLAICLTYVFIVKVLGPKLMENRKPFELKQ 64
+MS + + + ++ +V L PKLMEN P E+KQ
Sbjct: 150 IMSIVKSPMGLMVGFMVVVVFLMPKLMENIDPEEMKQ 186
>At1g15520.1 68414.m01867 ABC transporter family protein similar to
ABC1 protein GI:14331118 from [Nicotiana
plumbaginifolia]
Length = 1423
Score = 27.9 bits (59), Expect = 8.2
Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 1/51 (1%)
Query: 204 PRAFVWWIGMHAVL-FYYLFSDFYKQAYLRKSKKAKSMMKVEDEPVKYETK 253
P A+ +WIG A+L F LF+ + A + K + +EP ET+
Sbjct: 746 PHAYWYWIGTGALLGFVVLFNFGFTLALTFLNSLGKPQAVIAEEPASDETE 796
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.330 0.141 0.469
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,377,678
Number of Sequences: 28952
Number of extensions: 248372
Number of successful extensions: 753
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 746
Number of HSP's gapped (non-prelim): 6
length of query: 283
length of database: 12,070,560
effective HSP length: 80
effective length of query: 203
effective length of database: 9,754,400
effective search space: 1980143200
effective search space used: 1980143200
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.8 bits)
S2: 59 (27.9 bits)
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