Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000268-TA|BGIBMGA000268-PA|IPR000618|Insect cuticle
protein
         (166 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g25900.1 68417.m03724 aldose 1-epimerase family protein simil...    30   0.69 
At5g61210.1 68418.m07678 SNAP25 homologous protein SNAP33 (SNAP3...    28   2.8  
At2g21440.1 68415.m02551 RNA recognition motif (RRM)-containing ...    28   2.8  
At4g38480.1 68417.m05438 transducin family protein / WD-40 repea...    27   8.4  
At4g30530.1 68417.m04334 defense-related protein, putative stron...    27   8.4  
At4g18010.1 68417.m02679 inositol polyphosphate 5-phosphatase II...    27   8.4  
At4g09310.1 68417.m01539 SPla/RYanodine receptor (SPRY) domain-c...    27   8.4  
At4g09250.1 68417.m01532 SPla/RYanodine receptor (SPRY) domain-c...    27   8.4  
At4g09200.1 68417.m01522 SPla/RYanodine receptor (SPRY) domain-c...    27   8.4  
At1g52030.2 68414.m05870 myrosinase-binding protein, putative (F...    27   8.4  
At1g52030.1 68414.m05869 myrosinase-binding protein, putative (F...    27   8.4  

>At4g25900.1 68417.m03724 aldose 1-epimerase family protein similar
           to apospory-associated protein C; APOC [Chlamydomonas
           reinhardtii] GI:6970044 Pfam profile PF01263: Aldose
           1-epimerase
          Length = 318

 Score = 30.3 bits (65), Expect = 0.69
 Identities = 16/47 (34%), Positives = 24/47 (51%)

Query: 73  VLAHREDQEIAYPKYEYNYSVADGHSGDNKSQQEVRDGDVVKGSYSF 119
           V +  ED +I   K+EY   VA GH GD      V++ D    +++F
Sbjct: 137 VRSSNEDLKIWPHKFEYRLRVALGHDGDLTLTSRVKNTDTKPFNFTF 183


>At5g61210.1 68418.m07678 SNAP25 homologous protein SNAP33 (SNAP33)
           (SNAP33B) / synaptosomal-associated protein SNAP25-like
           1 / snap25a identical to SNAP25 homologous protein
           SNAP33 (AtSNAP33) (Synaptosomal-associated protein
           SNAP25-like 1) (SNAP-25 like protein 1) (Snap25a)
           (Swiss-Prot:Q9S7P9) [Arabidopsis thaliana]
          Length = 300

 Score = 28.3 bits (60), Expect = 2.8
 Identities = 18/73 (24%), Positives = 29/73 (39%)

Query: 86  KYEYNYSVADGHSGDNKSQQEVRDGDVVKGSYSFHEADGSIRTVEYTADDHNGFNAVVHN 145
           KY+Y  +  D    +N+S QE+    V K   +     G ++  E    D      ++H+
Sbjct: 80  KYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSVQGCLKVAEDIRSDATRTLVMLHD 139

Query: 146 TAPTSAPTLIKAV 158
                  T  KAV
Sbjct: 140 QGEQITRTHHKAV 152


>At2g21440.1 68415.m02551 RNA recognition motif (RRM)-containing
           protein contains InterPro entry IPR000504: RNA-binding
           region RNP-1 (RNA recognition motif) (RRM)
          Length = 1003

 Score = 28.3 bits (60), Expect = 2.8
 Identities = 20/69 (28%), Positives = 29/69 (42%), Gaps = 5/69 (7%)

Query: 83  AYPKYEYNYSVADGHSGDNKSQQEVRDGDVVKGSYSFHEADGSIRT----VEYTADDHNG 138
           A PK  YN   AD  +      +E  DGD    S    E D ++ +     + T DD +G
Sbjct: 407 AVPKNIYN-GAADATTASADGDKEGSDGDSENSSVDLEEVDEAVESHPPPGDDTDDDEDG 465

Query: 139 FNAVVHNTA 147
            N +  + A
Sbjct: 466 SNKLTESDA 474


>At4g38480.1 68417.m05438 transducin family protein / WD-40 repeat
           family protein contains contains Pfam PF00400: WD
           domain, G-beta repeat (7 copies, 3 weak);similar to gene
           PC326 protein - mouse, PIR2:S37694
          Length = 471

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 5/53 (9%)

Query: 85  PKYEYNYSVADGHSGDNKSQQEVRDGDVVKGSYSFHEADGSIRTVEYTADDHN 137
           P++   Y V D    D++S +E  D D      S  E +G +  VE T DD N
Sbjct: 402 PRWFDGYYVDDDDDSDDESSEESSDDD----DSSEEEENGEV-DVEITKDDDN 449


>At4g30530.1 68417.m04334 defense-related protein, putative strong
           similarity to defense-related protein [Brassica
           carinata] GI:14009290; contains Pfam profile PF00117:
           glutamine amidotransferase class-I
          Length = 250

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 22/84 (26%), Positives = 33/84 (39%), Gaps = 1/84 (1%)

Query: 71  AKVLAHREDQEIAYPKYEYNYSVADGHSGDNKS-QQEVRDGDVVKGSYSFHEADGSIRTV 129
           AKVLA+ ++ E+     E +     GH   NK    E+ D  +  G      AD +  T+
Sbjct: 166 AKVLAYSKNYEVEMYSIEDHLFCIQGHPEYNKEILFEIVDRVLALGYVKQEFADAAKATM 225

Query: 130 EYTADDHNGFNAVVHNTAPTSAPT 153
           E    D   +  +  N      PT
Sbjct: 226 ENRGADRKLWETICKNFLKGRVPT 249


>At4g18010.1 68417.m02679 inositol polyphosphate 5-phosphatase II
           (IP5PII) nearly identical to inositol polyphosphate
           5-phosphatase II [Arabidopsis thaliana] GI:10444263
           isoform contains an AT-acceptor splice site at intron 6
          Length = 646

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 12/27 (44%), Positives = 15/27 (55%), Gaps = 1/27 (3%)

Query: 95  DGHSGDNKSQQEVRDGDVVKGSYSFHE 121
           D   GD+ SQ E  DGD ++ SY   E
Sbjct: 349 DSSDGDSSSQDE-EDGDKIRNSYGLPE 374


>At4g09310.1 68417.m01539 SPla/RYanodine receptor (SPRY)
           domain-containing protein low similarity to RanBPM [Homo
           sapiens] GI:15080674; contains Pfam profile PF00622:
           SPRY domain
          Length = 397

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 2/53 (3%)

Query: 100 DNKSQQEVRDGDV--VKGSYSFHEADGSIRTVEYTADDHNGFNAVVHNTAPTS 150
           +N+ ++E+R   +  V GS  F        + +YT  DH+G      N APT+
Sbjct: 3   ENEEEEELRPTALYTVGGSDEFTFVSPDGLSGQYTGPDHDGVVLQTENPAPTN 55


>At4g09250.1 68417.m01532 SPla/RYanodine receptor (SPRY)
           domain-containing protein low similarity to RanBPM [Homo
           sapiens] GI:15080674; contains Pfam profile PF00622:
           SPRY domain
          Length = 427

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 2/53 (3%)

Query: 100 DNKSQQEVRDGDV--VKGSYSFHEADGSIRTVEYTADDHNGFNAVVHNTAPTS 150
           +N+ ++E+R   +  V GS  F        + +YT  DH+G      N APT+
Sbjct: 81  ENEEEEELRPTALYTVGGSDEFTFVSPDGLSGQYTGPDHDGVVLQTENPAPTN 133


>At4g09200.1 68417.m01522 SPla/RYanodine receptor (SPRY)
           domain-containing protein low similarity to RanBPM [Homo
           sapiens] GI:15080674; contains Pfam profile PF00622:
           SPRY domain
          Length = 397

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 2/53 (3%)

Query: 100 DNKSQQEVRDGDV--VKGSYSFHEADGSIRTVEYTADDHNGFNAVVHNTAPTS 150
           +N+ ++E+R   +  V GS  F        + +YT  DH+G      N APT+
Sbjct: 3   ENEEEEELRPTALYTVGGSDEFTFVSPDGLSGQYTGPDHDGVVLQTENPAPTN 55


>At1g52030.2 68414.m05870 myrosinase-binding protein, putative
           (F-ATMBP) identical to SP|Q9SAV1 Myrosinase binding
           protein-like f-AtMBP [Arabidopsis thaliana]; similar to
           myrosinase binding protein GI:1711295 from [Brassica
           napus]; contains Pfam PF01419: Jacalin-like lectin
           domain; identical to cDNA myrosinase-binding
           protein-like protein (MBP1.2)  GI:6760446
          Length = 642

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 9/75 (12%)

Query: 70  IAKVLAHREDQEIAYPKYEYNYSVADGHSGDNKSQQEVRDGDVVKGSYSFH-EADGSIRT 128
           + KV   + D  +AY K+EY          D K +        V G+  F  E+D  I +
Sbjct: 356 VRKVYIGQGDSGVAYVKFEYR--------KDGKRETREHGKMTVLGTEEFEVESDDYITS 407

Query: 129 VEYTADDHNGFNAVV 143
           +E + D+  GF + +
Sbjct: 408 IEVSVDNVFGFKSEI 422


>At1g52030.1 68414.m05869 myrosinase-binding protein, putative
           (F-ATMBP) identical to SP|Q9SAV1 Myrosinase binding
           protein-like f-AtMBP [Arabidopsis thaliana]; similar to
           myrosinase binding protein GI:1711295 from [Brassica
           napus]; contains Pfam PF01419: Jacalin-like lectin
           domain; identical to cDNA myrosinase-binding
           protein-like protein (MBP1.2)  GI:6760446
          Length = 642

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 9/75 (12%)

Query: 70  IAKVLAHREDQEIAYPKYEYNYSVADGHSGDNKSQQEVRDGDVVKGSYSFH-EADGSIRT 128
           + KV   + D  +AY K+EY          D K +        V G+  F  E+D  I +
Sbjct: 356 VRKVYIGQGDSGVAYVKFEYR--------KDGKRETREHGKMTVLGTEEFEVESDDYITS 407

Query: 129 VEYTADDHNGFNAVV 143
           +E + D+  GF + +
Sbjct: 408 IEVSVDNVFGFKSEI 422


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.314    0.129    0.370 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,289,077
Number of Sequences: 28952
Number of extensions: 112770
Number of successful extensions: 190
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 188
Number of HSP's gapped (non-prelim): 11
length of query: 166
length of database: 12,070,560
effective HSP length: 76
effective length of query: 90
effective length of database: 9,870,208
effective search space: 888318720
effective search space used: 888318720
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
S2: 56 (26.6 bits)