BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000226-TA|BGIBMGA000226-PA|IPR000618|Insect cuticle
protein
(398 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g07530.1 68417.m01179 hypothetical protein 32 0.58
At2g38410.1 68415.m04718 VHS domain-containing protein / GAT dom... 31 1.0
At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low sim... 31 1.3
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 30 2.4
At5g11690.1 68418.m01366 mitochondrial import inner membrane tra... 30 3.1
At4g00010.1 68417.m00001 expressed protein 30 3.1
At3g49840.1 68416.m05449 proline-rich family protein contains pr... 29 4.1
At2g25970.1 68415.m03117 KH domain-containing protein 29 4.1
At1g52695.1 68414.m05951 phospholipase/carboxylesterase family p... 29 4.1
At2g07690.1 68415.m00993 minichromosome maintenance family prote... 29 7.2
At5g61030.1 68418.m07659 RNA-binding protein, putative similar t... 28 9.5
At5g07170.1 68418.m00817 hypothetical protein 28 9.5
At5g01550.1 68418.m00070 lectin protein kinase, putative similar... 28 9.5
At4g20720.1 68417.m03011 dentin sialophosphoprotein-related cont... 28 9.5
>At4g07530.1 68417.m01179 hypothetical protein
Length = 818
Score = 32.3 bits (70), Expect = 0.58
Identities = 26/73 (35%), Positives = 33/73 (45%), Gaps = 3/73 (4%)
Query: 173 DTGFHVSSAHPDNPGVIGSP-FHRAPLVRGETKARGRTAVQRGLDGSYR-FISAGPDQRR 230
+TG ++ DN V GS R+ V K R RT +RG DG+Y + A R
Sbjct: 395 NTGTSAAAGQSDNNQVSGSSAVVRSDGVPSSPK-RARTQTERGEDGTYGCYSGAAASADR 453
Query: 231 TESSDSQGNVRGS 243
S S G RGS
Sbjct: 454 RSSGRSHGGGRGS 466
>At2g38410.1 68415.m04718 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 671
Score = 31.5 bits (68), Expect = 1.0
Identities = 25/75 (33%), Positives = 30/75 (40%), Gaps = 3/75 (4%)
Query: 315 FKGPEGTAASGHVKPPPFQVSESEIPE-GP-AGPITSAETDNAPNLQTDGGYSQPAFSQS 372
F GPEG + + S E P P A PI + + P Q GGY P +
Sbjct: 119 FGGPEGKYPQYYWAYDELRRSGVEFPRRSPDASPIITPPVSHPPLRQPQGGYGVPP-AGY 177
Query: 373 GNEDLGYVDEDAGYG 387
G GY AGYG
Sbjct: 178 GVHQAGYGVPQAGYG 192
>At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low
similarity to breast cancer susceptibility protein
[Gallus gallus] GI:19568157; contains Pfam profile
PF00634: BRCA2 repeat
Length = 1155
Score = 31.1 bits (67), Expect = 1.3
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Query: 221 FISAGPDQRRTESSDSQGNVRGSYTFLDDKGVQRTVNYIAGPGIGY-RIIKNNKDA 275
F++ G Q E S+S + S F+DD V + + G +G+ +IK KDA
Sbjct: 1034 FVTDGSTQHSGEISNSLLAISFSTPFMDDSSVSHISHNLVGSVVGFCNLIKRAKDA 1089
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 30.3 bits (65), Expect = 2.4
Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 3/56 (5%)
Query: 74 DPATDTVKETIYTAGPRGFRAKGPNVHRQLDLDQRP--RGPIGNKDDPFFDPNEDP 127
DP + +ET+ R +K PN H +L ++ RP G DD P +DP
Sbjct: 559 DPVV-SFRETVCDRSTRTVMSKSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDP 613
>At5g11690.1 68418.m01366 mitochondrial import inner membrane
translocase subunit Tim17, putative similar to
SP|Q9SP35 Mitochondrial import inner membrane
translocase subunit TIM17 {Arabidopsis thaliana};
contains Pfam profile PF02466: Mitochondrial import
inner membrane translocase subunit Tim17
Length = 133
Score = 29.9 bits (64), Expect = 3.1
Identities = 20/54 (37%), Positives = 24/54 (44%), Gaps = 3/54 (5%)
Query: 25 KSRANDGDYNTVNTDGSFDFGYANKDRGSSYHLAHGTANGVVGGRF-GGKDPAT 77
K G Y VN G + FG A GS YH G N +G R+ GG A+
Sbjct: 5 KKSKEHGLYRIVNAIG-YAFG-AGAVGGSVYHFVRGAYNSPIGARYVGGTQAAS 56
>At4g00010.1 68417.m00001 expressed protein
Length = 340
Score = 29.9 bits (64), Expect = 3.1
Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Query: 221 FISAGPDQRRTESSDSQGNVRGSYTFLDDKGVQRTVNYIAGPGIGY-RIIKNNKD 274
F++ G Q E S+S + S +F+DD V + + G +G+ +IK KD
Sbjct: 219 FVTDGSAQHSGEISNSLLAISFSTSFMDDSSVSHISHNLVGSVVGFCNLIKRAKD 273
>At3g49840.1 68416.m05449 proline-rich family protein contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 606
Score = 29.5 bits (63), Expect = 4.1
Identities = 12/32 (37%), Positives = 15/32 (46%)
Query: 363 GYSQPAFSQSGNEDLGYVDEDAGYGGSKPTKG 394
GY P + Q+G GY GYG P +G
Sbjct: 516 GYPPPQYPQAGYPPAGYPPPQQGYGQGYPAQG 547
>At2g25970.1 68415.m03117 KH domain-containing protein
Length = 632
Score = 29.5 bits (63), Expect = 4.1
Identities = 17/59 (28%), Positives = 22/59 (37%)
Query: 337 SEIPEGPAGPITSAETDNAPNLQTDGGYSQPAFSQSGNEDLGYVDEDAGYGGSKPTKGP 395
S G AG T+ + A GY P SQ+G ++GYG P P
Sbjct: 479 SSAQSGQAGYGTTGQQPPAQGSTGQAGYGAPPTSQAGYSSQPAAAYNSGYGAPPPASKP 537
>At1g52695.1 68414.m05951 phospholipase/carboxylesterase family
protein contains Pfam profile: PF02230
phospholipase/carboxylesterase; supported by full length
cDNA gi:26450919 from [Arabidopsis thaliana]
Length = 231
Score = 29.5 bits (63), Expect = 4.1
Identities = 16/40 (40%), Positives = 22/40 (55%), Gaps = 4/40 (10%)
Query: 32 DYNTVNTDGSFDFGYANKDRGSSYHLAHGTANGVVGGRFG 71
+YN NT+ FG AN+ S + HGT++ VV FG
Sbjct: 159 EYNMNNTN----FGTANRAAASKILILHGTSDDVVPSSFG 194
>At2g07690.1 68415.m00993 minichromosome maintenance family protein
/ MCM family protein similar to SP|P55862 DNA
replication licensing factor MCM5 (CDC46 homolog)
{Xenopus laevis}; contains Pfam profile PF00493:
MCM2/3/5 family
Length = 727
Score = 28.7 bits (61), Expect = 7.2
Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Query: 236 SQGNVRGSYTFLDDKGVQRTVNYIAGPGIGYRIIKNNKDAIPSYFPTLPSPYDP 289
S+ + +Y FL K ++T PG+G I+ + D IP P P DP
Sbjct: 156 SRVKAKATYVFLVCKNCKKTREVPCRPGLGGAIVPRSCDNIPQPGEE-PCPLDP 208
>At5g61030.1 68418.m07659 RNA-binding protein, putative similar to
RNA-binding protein from [Solanum tuberosum]
GI:15822705, [Nicotiana tabacum] GI:15822703, [Nicotiana
sylvestris] GI:624925; contains InterPro entry
IPR000504: RNA-binding region RNP-1 (RNA recognition
motif) (RRM)
Length = 309
Score = 28.3 bits (60), Expect = 9.5
Identities = 21/66 (31%), Positives = 28/66 (42%), Gaps = 7/66 (10%)
Query: 33 YNTVNTDGSFDFGYANKDR------GSSYHLAHGTANGVVGGRFGGKDPATDTVK-ETIY 85
Y + NT GS + G+A + G+S GG+FGG+D K ET
Sbjct: 234 YGSANTYGSSNGGFAGDSQFGGSPVGNSSQFGGDNTQFTAGGQFGGEDQFGSMEKSETKM 293
Query: 86 TAGPRG 91
GP G
Sbjct: 294 EDGPIG 299
>At5g07170.1 68418.m00817 hypothetical protein
Length = 542
Score = 28.3 bits (60), Expect = 9.5
Identities = 15/49 (30%), Positives = 22/49 (44%), Gaps = 2/49 (4%)
Query: 4 SSGSVYFVCFCALVRTQVIPG--KSRANDGDYNTVNTDGSFDFGYANKD 50
+S S+ CFC L+ + G K D +T T S+D G + D
Sbjct: 13 TSNSLLMFCFCNLIIVMIFTGSLKPGLKDSQDSTFVTSVSYDMGNISSD 61
>At5g01550.1 68418.m00070 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 688
Score = 28.3 bits (60), Expect = 9.5
Identities = 12/31 (38%), Positives = 16/31 (51%)
Query: 353 DNAPNLQTDGGYSQPAFSQSGNEDLGYVDED 383
D+ P + D GYS + S G+ GYV D
Sbjct: 635 DDVPEIDNDWGYSDSSRSDLGSNFEGYVSSD 665
>At4g20720.1 68417.m03011 dentin sialophosphoprotein-related
contains weak similarity to dentin sialophosphoprotein
precursor (Swiss-Prot:Q9NZW4) [Homo sapiens]
Length = 729
Score = 28.3 bits (60), Expect = 9.5
Identities = 37/127 (29%), Positives = 51/127 (40%), Gaps = 10/127 (7%)
Query: 116 KDDPFFDPNEDPSYAFNFDTRTYSKNENADSRG--DVKGHYSYVDDIGERHDVSYIAGRD 173
+DD F DP S + ++ ++ D+ G D K V GE ++S AGRD
Sbjct: 195 EDDDFKDPRS-LSLFDSVKSQGVVGSQQHDNVGLFDKKDAPKSVVSSGEHENLSLFAGRD 253
Query: 174 TGFHVSSAHPDNPGVIGSPFHRAPLVRGETKA--RGRTAVQR----GLDGSYRFISAGPD 227
VS A N G R E + G+ QR +D S+ F G D
Sbjct: 254 AQESVSFAAQGNFGFFEEKDARNSFKEDENLSLFEGKEDAQRTSSSKVDESFGFFE-GKD 312
Query: 228 QRRTESS 234
+RT SS
Sbjct: 313 AQRTSSS 319
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.313 0.136 0.413
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,965,059
Number of Sequences: 28952
Number of extensions: 521734
Number of successful extensions: 869
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 864
Number of HSP's gapped (non-prelim): 17
length of query: 398
length of database: 12,070,560
effective HSP length: 83
effective length of query: 315
effective length of database: 9,667,544
effective search space: 3045276360
effective search space used: 3045276360
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)
S2: 60 (28.3 bits)
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