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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000224-TA|BGIBMGA000224-PA|IPR005828|General substrate
transporter, IPR007114|Major facilitator superfamily
         (322 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_16011| Best HMM Match : Sugar_tr (HMM E-Value=0)                    96   3e-20
SB_2499| Best HMM Match : Sugar_tr (HMM E-Value=1e-28)                 62   6e-10
SB_8709| Best HMM Match : Sugar_tr (HMM E-Value=0)                     47   2e-05
SB_47688| Best HMM Match : ATP_synt_H (HMM E-Value=7)                  44   2e-04
SB_7879| Best HMM Match : Sugar_tr (HMM E-Value=0.03)                  39   0.006
SB_17113| Best HMM Match : Sugar_tr (HMM E-Value=0.015)                37   0.019
SB_57462| Best HMM Match : No HMM Matches (HMM E-Value=.)              34   0.14 
SB_1462| Best HMM Match : MFS_1 (HMM E-Value=0.042)                    34   0.14 
SB_43380| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   0.31 
SB_19714| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   0.72 
SB_28247| Best HMM Match : Na_Ca_ex (HMM E-Value=2e-27)                30   2.2  
SB_1828| Best HMM Match : DUF938 (HMM E-Value=1.5)                     30   2.9  
SB_51145| Best HMM Match : RCSD (HMM E-Value=1.3)                      29   3.9  
SB_18740| Best HMM Match : MFS_1 (HMM E-Value=1.1e-15)                 29   3.9  
SB_31847| Best HMM Match : Sugar_tr (HMM E-Value=4.6e-05)              29   5.1  
SB_41412| Best HMM Match : RRM_1 (HMM E-Value=2.9e-35)                 29   6.7  
SB_15719| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   8.9  

>SB_16011| Best HMM Match : Sugar_tr (HMM E-Value=0)
          Length = 512

 Score = 96.3 bits (229), Expect = 3e-20
 Identities = 79/329 (24%), Positives = 146/329 (44%), Gaps = 43/329 (13%)

Query: 1   MRGVVAAMPNLFVAVGITIQAALGSVFKWRILCYICGTYSVVMLIANFFLPETPYFVLMK 60
           +RG++ ++  L V +G+ +  ++G V KWR L      +  ++++  FF+PETP + L  
Sbjct: 171 LRGMLGSVNQLAVTMGLLLAYSMGVVLKWRWLACSGAIFPALLVVLMFFVPETPRWSLSH 230

Query: 61  DTEDKAKKSLTRFRGRDYDIDAEMVQI------------IEFKSANNIENYFVAM----- 103
                A  ++  FRG + D++ E  +I             EF     ++  F+++     
Sbjct: 231 KRRRDALDAMMWFRGPEADVEEECYRIEATMDNTQSMSCAEFCRPAIMKPLFISIALMFF 290

Query: 104 -QFAGVTILMMWTIEVLKASKTSLDPRYGNIILGFTRLSTACLTTVLLFKVGRKALALVS 162
            QF G+  ++  +  +   +    D +  ++I+G  +     +  +++ K GRK L   +
Sbjct: 291 QQFCGINAILFNSASIFHQAGFQ-DSKAVSVIIGAVQFVGTGIACLVVDKAGRKLLLWTT 349

Query: 163 AIGVGIISVFLGLY---------------------IHYATRPTILPQLCFMAYMVF---A 198
           A+G+ +  + LG Y                     IH++     +  L   + +VF    
Sbjct: 350 ALGMTVSLIALGFYFELYIPTTQEQPTPTPALLESIHHSIPAGKISWLAITSIVVFNLVF 409

Query: 199 TFGYYTIPFVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAFTVLMFG 258
              +  +P++VM E+FPLQ R             L F  T  F  + D++    T   +G
Sbjct: 410 ALAWGPVPWLVMSEIFPLQARGIASSISTLCNWSLAFAVTKTFVNIEDAITIQGTYWFYG 469

Query: 259 IISLLSAVFLYYFLPETKGLTLREIEEYF 287
            +S L  +F+  F+PETKG TL +IE  F
Sbjct: 470 GLSFLGFLFVLMFVPETKGKTLEQIERLF 498


>SB_2499| Best HMM Match : Sugar_tr (HMM E-Value=1e-28)
          Length = 456

 Score = 62.1 bits (144), Expect = 6e-10
 Identities = 31/87 (35%), Positives = 50/87 (57%), Gaps = 2/87 (2%)

Query: 2   RGVVAAMPNLFVAVGITIQAALGSVFKWRILCYICGTYSVVMLIANF-FLPETPYFVLMK 60
           RG + ++  L +  GI I  A+G  F WR    + G++   +L+    F+PET  +++ K
Sbjct: 168 RGAMGSINQLGITAGILISYAIGYAFDWRWSA-VAGSFPAALLVVLMAFMPETARWLIAK 226

Query: 61  DTEDKAKKSLTRFRGRDYDIDAEMVQI 87
             E +A+K+L   RG DYDID E+ +I
Sbjct: 227 KKETRARKTLLWLRGPDYDIDKELCEI 253



 Score = 34.7 bits (76), Expect = 0.10
 Identities = 21/104 (20%), Positives = 49/104 (47%), Gaps = 7/104 (6%)

Query: 79  DIDAEMVQIIEFKSANNIENYFVAM------QFAGVTILMMWTIEVLKASKTSLDPRYGN 132
           D   +   + EFK+ + +  + ++M      QF+G+   M +   + + +    DP    
Sbjct: 322 DTQNQRFSLKEFKNPSLLRPFLISMSLHFFQQFSGINAFMFYCATIFQKAGFK-DPTGVP 380

Query: 133 IILGFTRLSTACLTTVLLFKVGRKALALVSAIGVGIISVFLGLY 176
           I++G  +   + ++  L+ + GR+ L +V+ +G+ I      +Y
Sbjct: 381 ILIGAVQFVASAISLALIDRGGRRFLLIVAGVGMSISCFTCAVY 424


>SB_8709| Best HMM Match : Sugar_tr (HMM E-Value=0)
          Length = 493

 Score = 46.8 bits (106), Expect = 2e-05
 Identities = 26/90 (28%), Positives = 46/90 (51%), Gaps = 7/90 (7%)

Query: 1   MRGVVAAMPNLFVAVGITIQAALGSVFK------WRILCYICGTYSVVMLIANFFLPETP 54
           MRG +  + NLF+  G  I + +  +F       WR +  +    S++M      LPE+P
Sbjct: 173 MRGKLVTLNNLFITGGQFIASVVDGIFAYDRQNGWRFMLGLAAVPSIIMFFGCVILPESP 232

Query: 55  YFVLMKDTEDKAKKSLTRFRGRDYDIDAEM 84
            +++ K    +A+ +L + RGR  D+D E+
Sbjct: 233 RWLISKCKYAEARAALCKIRGRT-DVDREL 261


>SB_47688| Best HMM Match : ATP_synt_H (HMM E-Value=7)
          Length = 124

 Score = 43.6 bits (98), Expect = 2e-04
 Identities = 22/86 (25%), Positives = 43/86 (50%)

Query: 207 FVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAFTVLMFGIISLLSAV 266
           ++++ E+FP  ++                  +F F  L  S+G + T   +G+I +++ V
Sbjct: 3   WLLLSEIFPSGIKGRAFSLATVLNWGTNVFVSFTFLDLLGSIGTSGTFFFYGLICVIAIV 62

Query: 267 FLYYFLPETKGLTLREIEEYFKSGRS 292
           F+Y ++PETK  TL +I     S ++
Sbjct: 63  FIYKYVPETKNKTLEQISAELNSKKA 88


>SB_7879| Best HMM Match : Sugar_tr (HMM E-Value=0.03)
          Length = 201

 Score = 38.7 bits (86), Expect = 0.006
 Identities = 14/43 (32%), Positives = 28/43 (65%)

Query: 250 FAFTVLMFGIISLLSAVFLYYFLPETKGLTLREIEEYFKSGRS 292
           + +T ++F  +  +   F ++F+PETKG T+ +I ++F+ G S
Sbjct: 141 YPYTFIVFMALVAIFWTFTFFFVPETKGRTIEDITDHFRGGDS 183


>SB_17113| Best HMM Match : Sugar_tr (HMM E-Value=0.015)
          Length = 292

 Score = 37.1 bits (82), Expect = 0.019
 Identities = 25/97 (25%), Positives = 51/97 (52%), Gaps = 4/97 (4%)

Query: 2   RGVVAAMPNLFVAVGIT-IQAALGSVFKWRILCYICGTYSVVMLIANFFLPETPYFVLMK 60
           R     + N+FV+VG + +   +  + +WR          + +LI  +F+PE+P +++ +
Sbjct: 84  RTAAGFISNMFVSVGFSSVTLFVYFIHEWRYQLVAVSATGLPLLIFWWFIPESPRWLIAE 143

Query: 61  DTEDKAKKSLTRFRGRD-YDIDAE--MVQIIEFKSAN 94
           +  D+A   LT+F  ++   +D++  M  I E K A+
Sbjct: 144 NKLDEAHALLTKFAEKNRVTVDSKHLMHMIREVKKAD 180


>SB_57462| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 127

 Score = 34.3 bits (75), Expect = 0.14
 Identities = 25/92 (27%), Positives = 43/92 (46%), Gaps = 11/92 (11%)

Query: 195 MVFATFGYYTIP------FVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSL 248
           M FA  G +TI       ++   EL+P  +R           ++   +     PY+ DS 
Sbjct: 11  MTFALIGKFTISAAYYQIYIHSAELYPTVIRTIGVGFA----SLCARVGAMGAPYVADST 66

Query: 249 GFAFTVLMFGIISLLSAVFLYYFLPETKGLTL 280
                 ++FG +S+ +A  + +FLPET+G+ L
Sbjct: 67  PLIAPAIIFGGLSM-TAGMVTFFLPETRGMPL 97


>SB_1462| Best HMM Match : MFS_1 (HMM E-Value=0.042)
          Length = 277

 Score = 34.3 bits (75), Expect = 0.14
 Identities = 24/86 (27%), Positives = 45/86 (52%), Gaps = 5/86 (5%)

Query: 203 YTIPFVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAF--TVLMFGII 260
           +T  +++  EL+P  +R            I   +A + F  L+D  G +    V++FG +
Sbjct: 28  WTTIYLITSELYPTTIRNTAIGTCATFARIGGLVAPY-FIMLKDLPGVSLYLPVIIFGCL 86

Query: 261 SLLSAVFLYYFLPETKGLTL-REIEE 285
            +L+A+  ++F+PET  + L + IEE
Sbjct: 87  CILAAI-AFFFIPETLKVPLAQSIEE 111


>SB_43380| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 509

 Score = 33.1 bits (72), Expect = 0.31
 Identities = 43/174 (24%), Positives = 73/174 (41%), Gaps = 17/174 (9%)

Query: 132 NIILGFTRLSTACLTTVLLFKVGRKALALVSAIGVGIISVFLGLYIHYATRPTILPQLCF 191
           N+ L    + TACL      K GRK L L+ A G  I+   + L+  Y   P + P +  
Sbjct: 89  NLALQLPAVLTACLLGTWSDKNGRKPLMLIVAFG-AIVDASVALFTVYTDGP-LYPLI-- 144

Query: 192 MAYMVFATFGYYTIPFVVM----CELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDS 247
           +   +    G+Y    + +     +  P + R            +   L  F        
Sbjct: 145 IGGGINGVMGFYPTMVLALLAYIADTTPPERRAIKLAVLEALAFLSGTLGHFSSGIYIHH 204

Query: 248 LGFAFTVLMFGIIS--LLSAVFLYYFLPETKGLTLREIEEYFKSGRSVRQSQTK 299
           LG+  T   +GI+S  L++ ++L +FLPE+  +  + IE+   SG+ +   Q K
Sbjct: 205 LGYKAT--FWGILSLHLINFIYLLFFLPES--MPKQRIEQ---SGKCLTLEQVK 251


>SB_19714| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 66

 Score = 31.9 bits (69), Expect = 0.72
 Identities = 13/49 (26%), Positives = 28/49 (57%)

Query: 252 FTVLMFGIISLLSAVFLYYFLPETKGLTLREIEEYFKSGRSVRQSQTKL 300
           +T ++F ++ L   +F Y+ +PETKG ++ +I   F+    +    ++L
Sbjct: 6   YTFIVFLVLVLGFWLFTYFLVPETKGRSIDDITRQFRGDSHITLDYSRL 54


>SB_28247| Best HMM Match : Na_Ca_ex (HMM E-Value=2e-27)
          Length = 236

 Score = 30.3 bits (65), Expect = 2.2
 Identities = 17/89 (19%), Positives = 43/89 (48%), Gaps = 1/89 (1%)

Query: 98  NYFVAMQFAGVT-ILMMWTIEVLKASKTSLDPRYGNIILGFTRLSTACLTTVLLFKVGRK 156
           ++ + + + GVT  +++W + V+  +    D   G  ++ F      CL+++ + + G  
Sbjct: 70  SFIICIVWIGVTSYVLVWMVTVIGYTFLIPDSVMGLSLVAFGSSVPDCLSSLFVARKGDG 129

Query: 157 ALALVSAIGVGIISVFLGLYIHYATRPTI 185
            +A+   +G  +  + L L I +  + T+
Sbjct: 130 DMAVSHTVGSNVFDILLCLGIPWLIKTTV 158


>SB_1828| Best HMM Match : DUF938 (HMM E-Value=1.5)
          Length = 206

 Score = 29.9 bits (64), Expect = 2.9
 Identities = 25/78 (32%), Positives = 35/78 (44%), Gaps = 7/78 (8%)

Query: 207 FVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAFTVLMFGIISL--LS 264
           +V   ELFP  +R                L +F  PY+ + L     +L FGI+++  L 
Sbjct: 62  YVYAAELFPTVIRNIGMGTSTAAAR----LGSFSAPYVVN-LNRIHPLLPFGIMAVKALL 116

Query: 265 AVFLYYFLPETKGLTLRE 282
           A  L   LPETKG+   E
Sbjct: 117 AGILCMTLPETKGMATAE 134


>SB_51145| Best HMM Match : RCSD (HMM E-Value=1.3)
          Length = 248

 Score = 29.5 bits (63), Expect = 3.9
 Identities = 24/77 (31%), Positives = 31/77 (40%), Gaps = 3/77 (3%)

Query: 207 FVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAFTVLMFGIISLLSAV 266
           +V   ELFP  VR            +   LA F     R +    F  LM G+  L  A 
Sbjct: 25  YVFSAELFPTMVRNSGMGLLSVISRVGAALAPFVVQLTRINAILPFA-LMGGLTFL--AA 81

Query: 267 FLYYFLPETKGLTLREI 283
              +FLPET+G    E+
Sbjct: 82  LACWFLPETRGKPTLEV 98


>SB_18740| Best HMM Match : MFS_1 (HMM E-Value=1.1e-15)
          Length = 839

 Score = 29.5 bits (63), Expect = 3.9
 Identities = 17/77 (22%), Positives = 40/77 (51%), Gaps = 1/77 (1%)

Query: 2   RGVVAAMPNLFVAVGITIQAALGSVFK-WRILCYICGTYSVVMLIANFFLPETPYFVLMK 60
           R  V  + N+FV++G  + + L  + + ++ L       +  +++    +PETP +++ +
Sbjct: 203 RTAVGFLTNIFVSLGFDMLSLLAYLIRDYQYLLLAVSLPAAPLVLFWGVIPETPRWLIAQ 262

Query: 61  DTEDKAKKSLTRFRGRD 77
           +  D+A K L  F  ++
Sbjct: 263 NRLDEAHKLLMDFASKN 279


>SB_31847| Best HMM Match : Sugar_tr (HMM E-Value=4.6e-05)
          Length = 549

 Score = 29.1 bits (62), Expect = 5.1
 Identities = 23/98 (23%), Positives = 47/98 (47%), Gaps = 3/98 (3%)

Query: 2   RGVVAAMPNLFVAVGITIQAALGS-VFKWRILCYICGTYSVVMLIANFFLPETPYFVLMK 60
           R ++ ++  +F  V + +   +   V +WR L         V +    ++PE+P +++ +
Sbjct: 146 RTMIGSVVEIFWPVCLLLHTLIAFLVHEWRNLLLAVSIPGFVFVCFWRYIPESPRWLVTR 205

Query: 61  DTEDKAKKSLTRFRGRDYDIDAEMVQ-IIEFKSANNIE 97
              D+A+  L RF G    ID + ++ +IE    + IE
Sbjct: 206 GRLDEAQAVLNRF-GSSNPIDQQALRGLIESIRESQIE 242


>SB_41412| Best HMM Match : RRM_1 (HMM E-Value=2.9e-35)
          Length = 1118

 Score = 28.7 bits (61), Expect = 6.7
 Identities = 17/64 (26%), Positives = 32/64 (50%), Gaps = 1/64 (1%)

Query: 11  LFVAVGITIQAALGSVFK-WRILCYICGTYSVVMLIANFFLPETPYFVLMKDTEDKAKKS 69
           +F  VG T+   +    + WR    I G  S++ L+    LPETP +++     ++A+  
Sbjct: 781 VFWTVGNTMIVVIAYFIRDWRWYMRISGLPSILFLVFWRLLPETPRWLIAHGRLEEARDV 840

Query: 70  LTRF 73
           L ++
Sbjct: 841 LIKY 844


>SB_15719| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 364

 Score = 28.3 bits (60), Expect = 8.9
 Identities = 14/42 (33%), Positives = 22/42 (52%)

Query: 9  PNLFVAVGITIQAALGSVFKWRILCYICGTYSVVMLIANFFL 50
          PN  + VGI I  A  S+    I+C +   Y  + L+ +FF+
Sbjct: 55 PNTAILVGIIIVIAFFSLIGNSIVCIVFCLYDQLRLVKHFFV 96


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.331    0.143    0.424 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,644,931
Number of Sequences: 59808
Number of extensions: 307889
Number of successful extensions: 879
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 861
Number of HSP's gapped (non-prelim): 24
length of query: 322
length of database: 16,821,457
effective HSP length: 82
effective length of query: 240
effective length of database: 11,917,201
effective search space: 2860128240
effective search space used: 2860128240
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.9 bits)
S2: 60 (28.3 bits)

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