BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000224-TA|BGIBMGA000224-PA|IPR005828|General substrate transporter, IPR007114|Major facilitator superfamily (322 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_16011| Best HMM Match : Sugar_tr (HMM E-Value=0) 96 3e-20 SB_2499| Best HMM Match : Sugar_tr (HMM E-Value=1e-28) 62 6e-10 SB_8709| Best HMM Match : Sugar_tr (HMM E-Value=0) 47 2e-05 SB_47688| Best HMM Match : ATP_synt_H (HMM E-Value=7) 44 2e-04 SB_7879| Best HMM Match : Sugar_tr (HMM E-Value=0.03) 39 0.006 SB_17113| Best HMM Match : Sugar_tr (HMM E-Value=0.015) 37 0.019 SB_57462| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.14 SB_1462| Best HMM Match : MFS_1 (HMM E-Value=0.042) 34 0.14 SB_43380| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.31 SB_19714| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 0.72 SB_28247| Best HMM Match : Na_Ca_ex (HMM E-Value=2e-27) 30 2.2 SB_1828| Best HMM Match : DUF938 (HMM E-Value=1.5) 30 2.9 SB_51145| Best HMM Match : RCSD (HMM E-Value=1.3) 29 3.9 SB_18740| Best HMM Match : MFS_1 (HMM E-Value=1.1e-15) 29 3.9 SB_31847| Best HMM Match : Sugar_tr (HMM E-Value=4.6e-05) 29 5.1 SB_41412| Best HMM Match : RRM_1 (HMM E-Value=2.9e-35) 29 6.7 SB_15719| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.9 >SB_16011| Best HMM Match : Sugar_tr (HMM E-Value=0) Length = 512 Score = 96.3 bits (229), Expect = 3e-20 Identities = 79/329 (24%), Positives = 146/329 (44%), Gaps = 43/329 (13%) Query: 1 MRGVVAAMPNLFVAVGITIQAALGSVFKWRILCYICGTYSVVMLIANFFLPETPYFVLMK 60 +RG++ ++ L V +G+ + ++G V KWR L + ++++ FF+PETP + L Sbjct: 171 LRGMLGSVNQLAVTMGLLLAYSMGVVLKWRWLACSGAIFPALLVVLMFFVPETPRWSLSH 230 Query: 61 DTEDKAKKSLTRFRGRDYDIDAEMVQI------------IEFKSANNIENYFVAM----- 103 A ++ FRG + D++ E +I EF ++ F+++ Sbjct: 231 KRRRDALDAMMWFRGPEADVEEECYRIEATMDNTQSMSCAEFCRPAIMKPLFISIALMFF 290 Query: 104 -QFAGVTILMMWTIEVLKASKTSLDPRYGNIILGFTRLSTACLTTVLLFKVGRKALALVS 162 QF G+ ++ + + + D + ++I+G + + +++ K GRK L + Sbjct: 291 QQFCGINAILFNSASIFHQAGFQ-DSKAVSVIIGAVQFVGTGIACLVVDKAGRKLLLWTT 349 Query: 163 AIGVGIISVFLGLY---------------------IHYATRPTILPQLCFMAYMVF---A 198 A+G+ + + LG Y IH++ + L + +VF Sbjct: 350 ALGMTVSLIALGFYFELYIPTTQEQPTPTPALLESIHHSIPAGKISWLAITSIVVFNLVF 409 Query: 199 TFGYYTIPFVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAFTVLMFG 258 + +P++VM E+FPLQ R L F T F + D++ T +G Sbjct: 410 ALAWGPVPWLVMSEIFPLQARGIASSISTLCNWSLAFAVTKTFVNIEDAITIQGTYWFYG 469 Query: 259 IISLLSAVFLYYFLPETKGLTLREIEEYF 287 +S L +F+ F+PETKG TL +IE F Sbjct: 470 GLSFLGFLFVLMFVPETKGKTLEQIERLF 498 >SB_2499| Best HMM Match : Sugar_tr (HMM E-Value=1e-28) Length = 456 Score = 62.1 bits (144), Expect = 6e-10 Identities = 31/87 (35%), Positives = 50/87 (57%), Gaps = 2/87 (2%) Query: 2 RGVVAAMPNLFVAVGITIQAALGSVFKWRILCYICGTYSVVMLIANF-FLPETPYFVLMK 60 RG + ++ L + GI I A+G F WR + G++ +L+ F+PET +++ K Sbjct: 168 RGAMGSINQLGITAGILISYAIGYAFDWRWSA-VAGSFPAALLVVLMAFMPETARWLIAK 226 Query: 61 DTEDKAKKSLTRFRGRDYDIDAEMVQI 87 E +A+K+L RG DYDID E+ +I Sbjct: 227 KKETRARKTLLWLRGPDYDIDKELCEI 253 Score = 34.7 bits (76), Expect = 0.10 Identities = 21/104 (20%), Positives = 49/104 (47%), Gaps = 7/104 (6%) Query: 79 DIDAEMVQIIEFKSANNIENYFVAM------QFAGVTILMMWTIEVLKASKTSLDPRYGN 132 D + + EFK+ + + + ++M QF+G+ M + + + + DP Sbjct: 322 DTQNQRFSLKEFKNPSLLRPFLISMSLHFFQQFSGINAFMFYCATIFQKAGFK-DPTGVP 380 Query: 133 IILGFTRLSTACLTTVLLFKVGRKALALVSAIGVGIISVFLGLY 176 I++G + + ++ L+ + GR+ L +V+ +G+ I +Y Sbjct: 381 ILIGAVQFVASAISLALIDRGGRRFLLIVAGVGMSISCFTCAVY 424 >SB_8709| Best HMM Match : Sugar_tr (HMM E-Value=0) Length = 493 Score = 46.8 bits (106), Expect = 2e-05 Identities = 26/90 (28%), Positives = 46/90 (51%), Gaps = 7/90 (7%) Query: 1 MRGVVAAMPNLFVAVGITIQAALGSVFK------WRILCYICGTYSVVMLIANFFLPETP 54 MRG + + NLF+ G I + + +F WR + + S++M LPE+P Sbjct: 173 MRGKLVTLNNLFITGGQFIASVVDGIFAYDRQNGWRFMLGLAAVPSIIMFFGCVILPESP 232 Query: 55 YFVLMKDTEDKAKKSLTRFRGRDYDIDAEM 84 +++ K +A+ +L + RGR D+D E+ Sbjct: 233 RWLISKCKYAEARAALCKIRGRT-DVDREL 261 >SB_47688| Best HMM Match : ATP_synt_H (HMM E-Value=7) Length = 124 Score = 43.6 bits (98), Expect = 2e-04 Identities = 22/86 (25%), Positives = 43/86 (50%) Query: 207 FVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAFTVLMFGIISLLSAV 266 ++++ E+FP ++ +F F L S+G + T +G+I +++ V Sbjct: 3 WLLLSEIFPSGIKGRAFSLATVLNWGTNVFVSFTFLDLLGSIGTSGTFFFYGLICVIAIV 62 Query: 267 FLYYFLPETKGLTLREIEEYFKSGRS 292 F+Y ++PETK TL +I S ++ Sbjct: 63 FIYKYVPETKNKTLEQISAELNSKKA 88 >SB_7879| Best HMM Match : Sugar_tr (HMM E-Value=0.03) Length = 201 Score = 38.7 bits (86), Expect = 0.006 Identities = 14/43 (32%), Positives = 28/43 (65%) Query: 250 FAFTVLMFGIISLLSAVFLYYFLPETKGLTLREIEEYFKSGRS 292 + +T ++F + + F ++F+PETKG T+ +I ++F+ G S Sbjct: 141 YPYTFIVFMALVAIFWTFTFFFVPETKGRTIEDITDHFRGGDS 183 >SB_17113| Best HMM Match : Sugar_tr (HMM E-Value=0.015) Length = 292 Score = 37.1 bits (82), Expect = 0.019 Identities = 25/97 (25%), Positives = 51/97 (52%), Gaps = 4/97 (4%) Query: 2 RGVVAAMPNLFVAVGIT-IQAALGSVFKWRILCYICGTYSVVMLIANFFLPETPYFVLMK 60 R + N+FV+VG + + + + +WR + +LI +F+PE+P +++ + Sbjct: 84 RTAAGFISNMFVSVGFSSVTLFVYFIHEWRYQLVAVSATGLPLLIFWWFIPESPRWLIAE 143 Query: 61 DTEDKAKKSLTRFRGRD-YDIDAE--MVQIIEFKSAN 94 + D+A LT+F ++ +D++ M I E K A+ Sbjct: 144 NKLDEAHALLTKFAEKNRVTVDSKHLMHMIREVKKAD 180 >SB_57462| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 127 Score = 34.3 bits (75), Expect = 0.14 Identities = 25/92 (27%), Positives = 43/92 (46%), Gaps = 11/92 (11%) Query: 195 MVFATFGYYTIP------FVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSL 248 M FA G +TI ++ EL+P +R ++ + PY+ DS Sbjct: 11 MTFALIGKFTISAAYYQIYIHSAELYPTVIRTIGVGFA----SLCARVGAMGAPYVADST 66 Query: 249 GFAFTVLMFGIISLLSAVFLYYFLPETKGLTL 280 ++FG +S+ +A + +FLPET+G+ L Sbjct: 67 PLIAPAIIFGGLSM-TAGMVTFFLPETRGMPL 97 >SB_1462| Best HMM Match : MFS_1 (HMM E-Value=0.042) Length = 277 Score = 34.3 bits (75), Expect = 0.14 Identities = 24/86 (27%), Positives = 45/86 (52%), Gaps = 5/86 (5%) Query: 203 YTIPFVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAF--TVLMFGII 260 +T +++ EL+P +R I +A + F L+D G + V++FG + Sbjct: 28 WTTIYLITSELYPTTIRNTAIGTCATFARIGGLVAPY-FIMLKDLPGVSLYLPVIIFGCL 86 Query: 261 SLLSAVFLYYFLPETKGLTL-REIEE 285 +L+A+ ++F+PET + L + IEE Sbjct: 87 CILAAI-AFFFIPETLKVPLAQSIEE 111 >SB_43380| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 509 Score = 33.1 bits (72), Expect = 0.31 Identities = 43/174 (24%), Positives = 73/174 (41%), Gaps = 17/174 (9%) Query: 132 NIILGFTRLSTACLTTVLLFKVGRKALALVSAIGVGIISVFLGLYIHYATRPTILPQLCF 191 N+ L + TACL K GRK L L+ A G I+ + L+ Y P + P + Sbjct: 89 NLALQLPAVLTACLLGTWSDKNGRKPLMLIVAFG-AIVDASVALFTVYTDGP-LYPLI-- 144 Query: 192 MAYMVFATFGYYTIPFVVM----CELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDS 247 + + G+Y + + + P + R + L F Sbjct: 145 IGGGINGVMGFYPTMVLALLAYIADTTPPERRAIKLAVLEALAFLSGTLGHFSSGIYIHH 204 Query: 248 LGFAFTVLMFGIIS--LLSAVFLYYFLPETKGLTLREIEEYFKSGRSVRQSQTK 299 LG+ T +GI+S L++ ++L +FLPE+ + + IE+ SG+ + Q K Sbjct: 205 LGYKAT--FWGILSLHLINFIYLLFFLPES--MPKQRIEQ---SGKCLTLEQVK 251 >SB_19714| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 66 Score = 31.9 bits (69), Expect = 0.72 Identities = 13/49 (26%), Positives = 28/49 (57%) Query: 252 FTVLMFGIISLLSAVFLYYFLPETKGLTLREIEEYFKSGRSVRQSQTKL 300 +T ++F ++ L +F Y+ +PETKG ++ +I F+ + ++L Sbjct: 6 YTFIVFLVLVLGFWLFTYFLVPETKGRSIDDITRQFRGDSHITLDYSRL 54 >SB_28247| Best HMM Match : Na_Ca_ex (HMM E-Value=2e-27) Length = 236 Score = 30.3 bits (65), Expect = 2.2 Identities = 17/89 (19%), Positives = 43/89 (48%), Gaps = 1/89 (1%) Query: 98 NYFVAMQFAGVT-ILMMWTIEVLKASKTSLDPRYGNIILGFTRLSTACLTTVLLFKVGRK 156 ++ + + + GVT +++W + V+ + D G ++ F CL+++ + + G Sbjct: 70 SFIICIVWIGVTSYVLVWMVTVIGYTFLIPDSVMGLSLVAFGSSVPDCLSSLFVARKGDG 129 Query: 157 ALALVSAIGVGIISVFLGLYIHYATRPTI 185 +A+ +G + + L L I + + T+ Sbjct: 130 DMAVSHTVGSNVFDILLCLGIPWLIKTTV 158 >SB_1828| Best HMM Match : DUF938 (HMM E-Value=1.5) Length = 206 Score = 29.9 bits (64), Expect = 2.9 Identities = 25/78 (32%), Positives = 35/78 (44%), Gaps = 7/78 (8%) Query: 207 FVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAFTVLMFGIISL--LS 264 +V ELFP +R L +F PY+ + L +L FGI+++ L Sbjct: 62 YVYAAELFPTVIRNIGMGTSTAAAR----LGSFSAPYVVN-LNRIHPLLPFGIMAVKALL 116 Query: 265 AVFLYYFLPETKGLTLRE 282 A L LPETKG+ E Sbjct: 117 AGILCMTLPETKGMATAE 134 >SB_51145| Best HMM Match : RCSD (HMM E-Value=1.3) Length = 248 Score = 29.5 bits (63), Expect = 3.9 Identities = 24/77 (31%), Positives = 31/77 (40%), Gaps = 3/77 (3%) Query: 207 FVVMCELFPLQVRXXXXXXXXXXFNILMFLATFCFPYLRDSLGFAFTVLMFGIISLLSAV 266 +V ELFP VR + LA F R + F LM G+ L A Sbjct: 25 YVFSAELFPTMVRNSGMGLLSVISRVGAALAPFVVQLTRINAILPFA-LMGGLTFL--AA 81 Query: 267 FLYYFLPETKGLTLREI 283 +FLPET+G E+ Sbjct: 82 LACWFLPETRGKPTLEV 98 >SB_18740| Best HMM Match : MFS_1 (HMM E-Value=1.1e-15) Length = 839 Score = 29.5 bits (63), Expect = 3.9 Identities = 17/77 (22%), Positives = 40/77 (51%), Gaps = 1/77 (1%) Query: 2 RGVVAAMPNLFVAVGITIQAALGSVFK-WRILCYICGTYSVVMLIANFFLPETPYFVLMK 60 R V + N+FV++G + + L + + ++ L + +++ +PETP +++ + Sbjct: 203 RTAVGFLTNIFVSLGFDMLSLLAYLIRDYQYLLLAVSLPAAPLVLFWGVIPETPRWLIAQ 262 Query: 61 DTEDKAKKSLTRFRGRD 77 + D+A K L F ++ Sbjct: 263 NRLDEAHKLLMDFASKN 279 >SB_31847| Best HMM Match : Sugar_tr (HMM E-Value=4.6e-05) Length = 549 Score = 29.1 bits (62), Expect = 5.1 Identities = 23/98 (23%), Positives = 47/98 (47%), Gaps = 3/98 (3%) Query: 2 RGVVAAMPNLFVAVGITIQAALGS-VFKWRILCYICGTYSVVMLIANFFLPETPYFVLMK 60 R ++ ++ +F V + + + V +WR L V + ++PE+P +++ + Sbjct: 146 RTMIGSVVEIFWPVCLLLHTLIAFLVHEWRNLLLAVSIPGFVFVCFWRYIPESPRWLVTR 205 Query: 61 DTEDKAKKSLTRFRGRDYDIDAEMVQ-IIEFKSANNIE 97 D+A+ L RF G ID + ++ +IE + IE Sbjct: 206 GRLDEAQAVLNRF-GSSNPIDQQALRGLIESIRESQIE 242 >SB_41412| Best HMM Match : RRM_1 (HMM E-Value=2.9e-35) Length = 1118 Score = 28.7 bits (61), Expect = 6.7 Identities = 17/64 (26%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Query: 11 LFVAVGITIQAALGSVFK-WRILCYICGTYSVVMLIANFFLPETPYFVLMKDTEDKAKKS 69 +F VG T+ + + WR I G S++ L+ LPETP +++ ++A+ Sbjct: 781 VFWTVGNTMIVVIAYFIRDWRWYMRISGLPSILFLVFWRLLPETPRWLIAHGRLEEARDV 840 Query: 70 LTRF 73 L ++ Sbjct: 841 LIKY 844 >SB_15719| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 364 Score = 28.3 bits (60), Expect = 8.9 Identities = 14/42 (33%), Positives = 22/42 (52%) Query: 9 PNLFVAVGITIQAALGSVFKWRILCYICGTYSVVMLIANFFL 50 PN + VGI I A S+ I+C + Y + L+ +FF+ Sbjct: 55 PNTAILVGIIIVIAFFSLIGNSIVCIVFCLYDQLRLVKHFFV 96 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.331 0.143 0.424 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,644,931 Number of Sequences: 59808 Number of extensions: 307889 Number of successful extensions: 879 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 861 Number of HSP's gapped (non-prelim): 24 length of query: 322 length of database: 16,821,457 effective HSP length: 82 effective length of query: 240 effective length of database: 11,917,201 effective search space: 2860128240 effective search space used: 2860128240 T: 11 A: 40 X1: 15 ( 7.2 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.9 bits) S2: 60 (28.3 bits)
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