Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000222-TA|BGIBMGA000222-PA|IPR006603|Cystinosin/ERS1p
repeat, IPR005282|Lysosomal cystine transporter
         (566 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g40670.1 68418.m04937 PQ-loop repeat family protein / transme...   228   7e-60
At2g41050.1 68415.m05069 PQ-loop repeat family protein / transme...    39   0.010
At4g07390.1 68417.m01134 PQ-loop repeat family protein / transme...    38   0.024
At4g20100.1 68417.m02941 PQ-loop repeat family protein / transme...    37   0.031
At4g36850.1 68417.m05225 PQ-loop repeat family protein / transme...    35   0.13 
At5g59470.1 68418.m07453 PQ-loop repeat family protein / transme...    31   1.5  
At1g17665.1 68414.m02187 expressed protein                             31   2.7  
At5g01100.1 68418.m00014 expressed protein similar to axi 1 [Nic...    30   3.6  
At4g27420.1 68417.m03941 ABC transporter family protein D.melano...    30   4.7  
At3g51550.1 68416.m05645 protein kinase family protein contains ...    30   4.7  

>At5g40670.1 68418.m04937 PQ-loop repeat family protein /
           transmembrane family protein similar to SP|O60931
           Cystinosin {Homo sapiens}; contains Pfam profile
           PF04193: PQ loop repeat
          Length = 270

 Score =  228 bits (558), Expect = 7e-60
 Identities = 111/234 (47%), Positives = 154/234 (65%), Gaps = 3/234 (1%)

Query: 254 ISY-IMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNCGLYFSK 312
           ISY I+GWI FA+WS+SFYPQ+ +NF+R+SVVGLNFDF+ LN+   + Y ++N  LYFS 
Sbjct: 11  ISYEIVGWIAFASWSISFYPQLILNFRRRSVVGLNFDFVMLNLTKHSSYMIYNVCLYFSP 70

Query: 313 EIQNEYFSRH-PRGLNPVQLNDVFFSLHAAFATLITITQCFLYEREEQRVSTVGRGILGA 371
            IQ +YF  +  + + PV  NDV FS+HA   T +T+ Q F+YER  Q+VS +  GI+  
Sbjct: 71  VIQKQYFDTYGDKEMIPVAANDVAFSIHAVVMTAVTLFQIFIYERGPQKVSRLAIGIVVV 130

Query: 372 FSAVVIVTGSIAVVGKIEWLDWLYYCSYVKLAITLIKYVPQAYMNYKRKSTVGWSIGNIF 431
                 +   IA+     WL  +   + +++ +T +KY+PQA MN+ RKSTVGWSIGNI 
Sbjct: 131 VWGFAAICFFIALPTH-SWLWLISIFNSIQVFMTCVKYIPQAKMNFTRKSTVGWSIGNIL 189

Query: 432 LDFVGGFLSILQMVLNAYNYNDWVSFFGDATKFGLGLFSLVFDIFFILQHYVFY 485
           LDF GG  + LQMV+ + + N W +F+G+  K  L L S+ FDI F+ QHYV Y
Sbjct: 190 LDFTGGLANYLQMVIQSIDQNSWKNFYGNMGKTLLSLISIFFDILFMFQHYVLY 243


>At2g41050.1 68415.m05069 PQ-loop repeat family protein /
           transmembrane family protein similar to SP|Q10482 Seven
           transmembrane protein 1 {Schizosaccharomyces pombe};
           contains Pfam profile PF04193: PQ loop repeat
          Length = 376

 Score = 38.7 bits (86), Expect = 0.010
 Identities = 20/61 (32%), Positives = 34/61 (55%), Gaps = 1/61 (1%)

Query: 246 MHSNEINIISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFN 305
           +HS+  + +S  +G I   +W V+  PQI  N+  KS  GL+  FL   ++G  +++L  
Sbjct: 3   LHSSFRDGLSLSLGIISVISWGVAEIPQIMTNYSEKSTEGLSITFLTTWMIG-DIFNLLG 61

Query: 306 C 306
           C
Sbjct: 62  C 62


>At4g07390.1 68417.m01134 PQ-loop repeat family protein /
           transmembrane family protein similar to SP|Q60441
           Mannose-P-dolichol utilization defect 1 protein
           (Suppressor of Lec15 and Lec35 glycosylation mutation)
           {Cricetulus griseus}, Lec35 protein [Cricetulus griseus]
           GI:9858721; contains Pfam profile PF04193: PQ loop
           repeat
          Length = 235

 Score = 37.5 bits (83), Expect = 0.024
 Identities = 50/189 (26%), Positives = 79/189 (41%), Gaps = 27/189 (14%)

Query: 253 IISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNCGLYFSK 312
           +IS ++G+   AA      PQI    + KSV GL+     L ++G+T+ SL  C      
Sbjct: 29  LISKLLGYCLVAASITVKLPQIMKIVQHKSVRGLSVVAFELEVVGYTI-SLAYC------ 81

Query: 313 EIQNEYFSRHPRGLNPVQLNDVFFSLHAAFATLITITQCFLYEREEQRVSTVGRGILGAF 372
                    H +GL      ++ F L  A    + +  C  Y  +   V+T  R +L   
Sbjct: 82  --------LH-KGLPFSAFGEMAFLLIQA----LILVACIYYYSQPVPVTTWIRPLLYCA 128

Query: 373 SAVVIVTGSIAVVGKIEWLDWLYYCSYVKLAITLIKYVPQAYMNYKRKSTVGWSIGNIFL 432
            A  ++ G I         + LY   +   AI L   +PQ + N+K KST   S    F+
Sbjct: 129 VAPTVLAGQINPT----LFEALYASQH---AIFLFARLPQIWKNFKNKSTGELSFLTFFM 181

Query: 433 DFVGGFLSI 441
           +F G  + +
Sbjct: 182 NFAGSIVRV 190


>At4g20100.1 68417.m02941 PQ-loop repeat family protein /
           transmembrane family protein similar to SP|Q10482 Seven
           transmembrane protein 1 {Schizosaccharomyces pombe};
           contains Pfam profile PF04193: PQ loop repeat
          Length = 288

 Score = 37.1 bits (82), Expect = 0.031
 Identities = 17/53 (32%), Positives = 32/53 (60%), Gaps = 1/53 (1%)

Query: 254 ISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNC 306
           +S  +G I   +WSV+  PQI  N+ +KS+ G++  FL   ++G  ++++  C
Sbjct: 6   LSLSLGIISVISWSVAEIPQIMTNYNQKSIEGVSITFLTTWMLG-DIFNVVGC 57



 Score = 29.5 bits (63), Expect = 6.2
 Identities = 16/47 (34%), Positives = 24/47 (51%)

Query: 241 LRITVMHSNEINIISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLN 287
           L ++  +  E N I   +GW   A +     PQI +N +R +V GLN
Sbjct: 166 LEVSSGNLGENNNIGMWLGWAMAAIYMGGRLPQICMNVRRGNVEGLN 212


>At4g36850.1 68417.m05225 PQ-loop repeat family protein /
           transmembrane family protein similar to SP|Q10482 Seven
           transmembrane protein 1 {Schizosaccharomyces pombe};
           contains Pfam profile PF04193: PQ loop repeat
          Length = 374

 Score = 35.1 bits (77), Expect = 0.13
 Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 1/53 (1%)

Query: 254 ISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNC 306
           +S+ +G      W V+  PQ+  NF+ KS  G++  FL   + G  +++L  C
Sbjct: 33  VSFALGIASLLCWGVAEIPQVITNFRTKSSNGVSLSFLLAWVAG-DIFNLVGC 84



 Score = 32.7 bits (71), Expect = 0.67
 Identities = 14/30 (46%), Positives = 19/30 (63%)

Query: 258 MGWIYFAAWSVSFYPQIYINFKRKSVVGLN 287
           +GW+  A +     PQI++N KR SV GLN
Sbjct: 262 LGWLMAAIYMGGRIPQIWLNIKRGSVEGLN 291


>At5g59470.1 68418.m07453 PQ-loop repeat family protein /
           transmembrane family protein similar to SP|Q60441
           Mannose-P-dolichol utilization defect 1 protein
           (Suppressor of Lec15 and Lec35 glycosylation mutation)
           (SL15) {Cricetulus griseus}; contains Pfam profile
           PF04193: PQ loop repeat
          Length = 239

 Score = 31.5 bits (68), Expect = 1.5
 Identities = 48/193 (24%), Positives = 77/193 (39%), Gaps = 27/193 (13%)

Query: 253 IISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNCGLYFSK 312
           +IS ++G+   AA      PQI      KSV GL+     L ++G+T+ SL  C    +K
Sbjct: 29  LISKLLGYFLVAASMTVKLPQIMKIVDNKSVKGLSVVAFELEVIGYTI-SLAYC---LNK 84

Query: 313 EIQNEYFSRHPRGLNPVQLNDVFFSLHAAFATLITITQCFLYEREEQRVSTVGRGILGAF 372
           ++    F             ++ F L  A    + +  C  Y  +   V+T  + IL   
Sbjct: 85  DLPFSAF------------GELAFLLIQA----LILVACIYYFSQPLSVTTWVKAILYFA 128

Query: 373 SAVVIVTGSIAVVGKIEWLDWLYYCSYVKLAITLIKYVPQAYMNYKRKSTVGWSIGNIFL 432
            A  +  G I       +L    Y S  K  I L   +PQ + N++ KST   S     +
Sbjct: 129 IAPTVFAGKID-----PFLFEALYAS--KHLIFLSARIPQIWKNFRNKSTGQLSFLTCLM 181

Query: 433 DFVGGFLSILQMV 445
           +F G    +   +
Sbjct: 182 NFGGALARVFTSI 194


>At1g17665.1 68414.m02187 expressed protein
          Length = 338

 Score = 30.7 bits (66), Expect = 2.7
 Identities = 21/86 (24%), Positives = 39/86 (45%), Gaps = 5/86 (5%)

Query: 99  KQITMAECSAVLRPRAAACSISSHRIRFCLVVMLILGIPSAHSQLDKMYVEVTDLNLLVE 158
           KQ+   E   VL PR  + SIS ++  F  +        +   ++ + Y ++   N    
Sbjct: 140 KQLGEGEDGLVLNPRTDSISISENKPEFVCLYPFTSTSSATQRKIKQQYDQLVKCN---- 195

Query: 159 ETRLLKLVQIGQY-NTSLEVISNIQH 183
             + L L Q+G++ N  +E  + +QH
Sbjct: 196 NAKGLTLAQVGEFANCLIEAKNELQH 221


>At5g01100.1 68418.m00014 expressed protein similar to axi 1
           [Nicotiana tabacum] GI:559921; contains Pfam profile
           PF03138: Plant protein family
          Length = 631

 Score = 30.3 bits (65), Expect = 3.6
 Identities = 13/64 (20%), Positives = 34/64 (53%), Gaps = 4/64 (6%)

Query: 392 DWLYYCSYVKLAITLIKYVPQAYMNYK--RKSTVGWSIGNIFLDFVGGFLSILQMVLNAY 449
           DW ++   +   + +++Y+PQ + + K   K+ V WS  + + + +   L   ++++  +
Sbjct: 271 DWKHFIKVLAEDVNIVEYLPQEFASIKPLEKNPVSWSKSSYYRNSISKLLKKHKVIV--F 328

Query: 450 NYND 453
           N+ D
Sbjct: 329 NHTD 332


>At4g27420.1 68417.m03941 ABC transporter family protein
           D.melanogaster P element CaSpeR-1 gene (white
           protein),PID:g870996
          Length = 639

 Score = 29.9 bits (64), Expect = 4.7
 Identities = 20/59 (33%), Positives = 29/59 (49%), Gaps = 4/59 (6%)

Query: 364 VGRGILGAFSAVVIVTGSIAVVGKIEWLDWL----YYCSYVKLAITLIKYVPQAYMNYK 418
           V  G+  A  A+V+   S   +G +  L +L    YY  +V + I+ IKYV   Y  YK
Sbjct: 511 VSGGLGLALGALVMDQKSATTLGSVIMLTFLLAGGYYVQHVPVFISWIKYVSIGYYTYK 569


>At3g51550.1 68416.m05645 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 895

 Score = 29.9 bits (64), Expect = 4.7
 Identities = 17/64 (26%), Positives = 32/64 (50%), Gaps = 7/64 (10%)

Query: 168 IGQYNTSLEVISNIQHPDIVQLVPPSIEIPGSNEPNKTVVISYDICAYGKSPGHSEVTFN 227
           + ++ T +E++S ++H  +V L+       G  E N  +++ YD  A+G    H   T N
Sbjct: 575 VHEFQTEIEMLSKLRHRHLVSLI-------GYCEENCEMILVYDYMAHGTMREHLYKTQN 627

Query: 228 VTPP 231
            + P
Sbjct: 628 PSLP 631


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.325    0.139    0.432 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,965,013
Number of Sequences: 28952
Number of extensions: 526256
Number of successful extensions: 1618
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1597
Number of HSP's gapped (non-prelim): 23
length of query: 566
length of database: 12,070,560
effective HSP length: 85
effective length of query: 481
effective length of database: 9,609,640
effective search space: 4622236840
effective search space used: 4622236840
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.6 bits)
S2: 62 (29.1 bits)