BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000222-TA|BGIBMGA000222-PA|IPR006603|Cystinosin/ERS1p repeat, IPR005282|Lysosomal cystine transporter (566 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17C9.10 |stm1||G-protein coupled receptor Stm1|Schizosacchar... 38 0.005 SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces ... 31 0.32 SPAC4C5.03 |||CTNS domain protein |Schizosaccharomyces pombe|chr... 31 0.43 SPBC354.09c |||Tre1 family protein |Schizosaccharomyces pombe|ch... 30 0.75 SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 30 0.99 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 29 1.7 SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 5.3 SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 27 9.2 >SPAC17C9.10 |stm1||G-protein coupled receptor Stm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 271 Score = 37.5 bits (83), Expect = 0.005 Identities = 24/95 (25%), Positives = 43/95 (45%), Gaps = 2/95 (2%) Query: 393 WLYYCSYVKLAITLIKYVPQAYMNYKRKSTVGWSIGNIFLDFVGGFLSILQMVLNAYNYN 452 W + + + +PQ N+K KST G SI L VG +++ + + Sbjct: 177 WPFTAGCISSVLYFCARIPQIIKNHKAKSTEGLSIIFFVLASVGNTSYAFSILV--FPAS 234 Query: 453 DWVSFFGDATKFGLGLFSLVFDIFFILQHYVFYRN 487 D++++ + LG FS +F +I ++ YRN Sbjct: 235 DYLNYTYANLPWILGAFSTIFLDIYIFYQFIKYRN 269 Score = 30.3 bits (65), Expect = 0.75 Identities = 20/75 (26%), Positives = 36/75 (48%), Gaps = 1/75 (1%) Query: 249 NEINIISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNCGL 308 +++N + G I + + PQI N K KS GL+ F L +G T Y+ F+ + Sbjct: 172 DDLNAWPFTAGCISSVLYFCARIPQIIKNHKAKSTEGLSIIFFVLASVGNTSYA-FSILV 230 Query: 309 YFSKEIQNEYFSRHP 323 + + + N ++ P Sbjct: 231 FPASDYLNYTYANLP 245 Score = 27.5 bits (58), Expect = 5.3 Identities = 13/50 (26%), Positives = 26/50 (52%) Query: 248 SNEINIISYIMGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMG 297 +N + +S +G + W V PQ+ N+K +S ++ FL + ++G Sbjct: 11 ANILTELSSFLGALSLGCWVVLLIPQLLENYKNQSGESISDLFLIIWLIG 60 >SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 31.5 bits (68), Expect = 0.32 Identities = 49/219 (22%), Positives = 94/219 (42%), Gaps = 28/219 (12%) Query: 266 WSVSFYPQIYINFKRKSVVGLNFDFLALNIMGFTMYSLFNCGLYFSKEIQNEYFSRHPRG 325 W V PQI N++ KS GL+ F+ ++ S++N ++Q + Sbjct: 30 WCVQLIPQIIKNYRAKSTEGLDTLFILSWVVASIPLSVYN-------QVQELNIALK--- 79 Query: 326 LNPVQLNDVFFSLHAAFATLITITQCFLYERE---EQRVSTVGRGIL--GAFSAVVIVTG 380 + P ++F +L AF T QC Y + + + V +L G A++I+T Sbjct: 80 VQP----ELFQAL--AFTTFF---QCLYYGSKWPLRKALFVVISFMLFSGGLQAMLILTI 130 Query: 381 SIAVVGKIEWLDWLYYCSYVKLAITLIKYVPQAYMNYKRKSTVGWSIGNIFLDFVGGFLS 440 + + +EW + + + + I ++PQ ++ ++ G S + +D G S Sbjct: 131 KLGIRRHVEWP--VVFMGVLATVLVNIGFLPQYISIFRARAVTGISYLFLAIDSSGSLFS 188 Query: 441 ILQMVLNAYNYNDWVSFFGDATKFGLGLFSLVFDIFFIL 479 L + + ++ V +G +G+F L F IF +L Sbjct: 189 FLSLPFDRWDVLAAVD-YGLLFIIEMGVFVLAF-IFNVL 225 Score = 27.9 bits (59), Expect = 4.0 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 2/48 (4%) Query: 258 MGWIYFAAWSVSFYPQIYINFKRKSVVGLNFDFLALNIMG--FTMYSL 303 MG + ++ F PQ F+ ++V G+++ FLA++ G F+ SL Sbjct: 145 MGVLATVLVNIGFLPQYISIFRARAVTGISYLFLAIDSSGSLFSFLSL 192 >SPAC4C5.03 |||CTNS domain protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 302 Score = 31.1 bits (67), Expect = 0.43 Identities = 26/108 (24%), Positives = 45/108 (41%), Gaps = 2/108 (1%) Query: 367 GILGAFSAVVIVTGSIAVVGKIEWLDWLYYCSYVKLAITLIKYVPQAYMNYKRKSTVGWS 426 G+LG F+ + + A V + W + + +++YVPQ + +S S Sbjct: 159 GLLGVFTWGLFIICLSATVLSSNFA-WAAFLGFSASFCAVVQYVPQIIKTIRHQSHGALS 217 Query: 427 IGNIFLDFVGGFL-SILQMVLNAYNYNDWVSFFGDATKFGLGLFSLVF 473 I + + GGFL L L N+ ++ + A GL L +F Sbjct: 218 IPMMMMQTPGGFLIGYLLSRLPGTNWTTYMMYIVSACLQGLLLMLCMF 265 >SPBC354.09c |||Tre1 family protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 794 Score = 30.3 bits (65), Expect = 0.75 Identities = 15/48 (31%), Positives = 24/48 (50%) Query: 193 SIEIPGSNEPNKTVVISYDICAYGKSPGHSEVTFNVTPPGFVNLDTVY 240 S+ I G + + +++ SY + Y + TF +P GFVN VY Sbjct: 197 SLSIDGDDSFHPSLIESYQVGGYDGVSIPTPATFGGSPSGFVNAPLVY 244 >SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 1496 Score = 29.9 bits (64), Expect = 0.99 Identities = 23/75 (30%), Positives = 34/75 (45%), Gaps = 5/75 (6%) Query: 307 GLYFSKEIQNEYFSRHPRGLNPVQLNDVFFS---LHAAFATLITITQCFLYEREEQRVST 363 G FS+E+Q +Y R G V NDV S H + T + + + E +ST Sbjct: 546 GSVFSEEVQEDYDERQFTGEAAVSANDVLSSSSPRHFSSHTPVNVDKSSYLEMNGHSLST 605 Query: 364 V--GRGILGAFSAVV 376 V G+ + +F A V Sbjct: 606 VISGKESISSFGAKV 620 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 29.1 bits (62), Expect = 1.7 Identities = 12/36 (33%), Positives = 22/36 (61%) Query: 469 FSLVFDIFFILQHYVFYRNADKKYNLDVKSLIHSFK 504 + L D++ + Q Y Y + ++K L++KS+ SFK Sbjct: 460 YRLNLDLYILKQFYCHYLDRNRKPPLNIKSVSESFK 495 >SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2100 Score = 27.5 bits (58), Expect = 5.3 Identities = 22/82 (26%), Positives = 32/82 (39%), Gaps = 5/82 (6%) Query: 132 LILGIPSAHSQLDKMYVEVTDLNLLVEETRLLKLVQIGQYNTSLEVISNIQHPDIVQLVP 191 LILGIPS +Y + ++ E T + K S ++N P + Sbjct: 792 LILGIPSLQLVRPDIYDSIREMIASNENTEVEK-----DKLDSFSALANPASPKAPEYTF 846 Query: 192 PSIEIPGSNEPNKTVVISYDIC 213 SI +P N PN I +C Sbjct: 847 VSIHVPPLNVPNAEGEIEESVC 868 >SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 26.6 bits (56), Expect = 9.2 Identities = 10/31 (32%), Positives = 20/31 (64%), Gaps = 2/31 (6%) Query: 256 YIMGWIYFAAWSVSFYPQIYINFKRKSVVGL 286 Y++ W+ AAW + Y + Y ++R +++GL Sbjct: 102 YVLFWLSMAAWVLQLYARSY--WQRDTLLGL 130 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.325 0.139 0.432 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,553,652 Number of Sequences: 5004 Number of extensions: 105659 Number of successful extensions: 368 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 355 Number of HSP's gapped (non-prelim): 16 length of query: 566 length of database: 2,362,478 effective HSP length: 76 effective length of query: 490 effective length of database: 1,982,174 effective search space: 971265260 effective search space used: 971265260 T: 11 A: 40 X1: 15 ( 7.0 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.6 bits) S2: 56 (26.6 bits)
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