BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000220-TA|BGIBMGA000220-PA|IPR003439|ABC transporter related, IPR013525|ABC-2 type transporter (823 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 88 4e-18 SPAPB24D3.09c |pdr1||ABC transporter Pdr1|Schizosaccharomyces po... 67 8e-12 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 34 0.092 SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosa... 32 0.37 SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pom... 32 0.37 SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 31 0.65 SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 31 0.85 SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr... 30 1.1 SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces po... 30 1.5 SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 29 2.6 SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 28 4.6 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 28 4.6 SPBC18E5.12c |mas2|SPBC23G7.02c|mitochondrial processing peptida... 28 4.6 SPCC126.02c |pku70||Ku domain protein Pku70|Schizosaccharomyces ... 28 4.6 SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy... 27 8.0 >SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transporter Bfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1530 Score = 88.2 bits (209), Expect = 4e-18 Identities = 66/244 (27%), Positives = 125/244 (51%), Gaps = 11/244 (4%) Query: 219 VVSGLSFEVKCGEILAVLATAQHEATGLLDVLAGVRKK--LSGDIVINGQPVASSTLRKV 276 +++G+ V G++ A++ + T LL+VLA ++GD+++NG+ + ST ++ Sbjct: 900 LLNGVQGFVVPGKLTALMGESGAGKTTLLNVLAQRVDTGVVTGDMLVNGRGL-DSTFQRR 958 Query: 277 VAYVRNDTALCPTMSVEHTLRFHAALRKPRNTHSHVKMGDRDRINLLIE-ELGLEQVRDT 335 YV+ +V LRF AALR+P + K + + L+E E E + T Sbjct: 959 TGYVQQQDVHIGESTVREALRFSAALRQPASVPLSEKYEYVESVIKLLEMESYAEAIIGT 1018 Query: 336 NVGRLTRSEIRRLNVACQLLLDMAILI-LDQPTKEMDIFDTFFLVEYLRNWASTGRVVIM 394 L + +R + +L A+L+ LD+PT +D + +V +LR A G+ ++ Sbjct: 1019 PGSGLNVEQRKRATIGVELAAKPALLLFLDEPTSGLDSQSAWSIVCFLRKLADAGQAILC 1078 Query: 395 SLHPPTYEIFAMLTKVVLI-SAGRTMFSG----YRRDMLPYFSSID-YPCPAFKNPSDYY 448 ++H P+ +F +++L+ G+T++ G + + +L YF S CP NP++Y Sbjct: 1079 TIHQPSAVLFDQFDRLLLLQKGGKTVYFGDIGEHSKTLLNYFESHGAVHCPDDGNPAEYI 1138 Query: 449 LDLV 452 LD++ Sbjct: 1139 LDVI 1142 Score = 43.2 bits (97), Expect = 1e-04 Identities = 36/169 (21%), Positives = 74/169 (43%), Gaps = 10/169 (5%) Query: 288 PTMSVEHTLRFHAALRKPRNTHSHVKMGD---RDRINLLIEELGLEQVRDTNVGR----- 339 P+++ TL F A R P N ++ + R+R +L+ GL +T VG Sbjct: 250 PSLTTAETLDFAAKCRTPNNRPCNLTRQEYVSRER-HLIATAFGLTHTFNTKVGNDFVRG 308 Query: 340 LTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWASTGRVV-IMSLHP 398 ++ E +R+ ++ I D T+ +D F V LR A+ ++ ++ + Sbjct: 309 VSGGERKRVTISEGFATRPTIACWDNSTRGLDSSTAFEFVNVLRTCANELKMTSFVTAYQ 368 Query: 399 PTYEIFAMLTKVVLISAGRTMFSGYRRDMLPYFSSIDYPCPAFKNPSDY 447 + +I+ + ++ ++ AGR ++ G YF + + C + D+ Sbjct: 369 ASEKIYKLFDRICVLYAGRQIYYGPADKAKQYFLDMGFDCHPRETTPDF 417 Score = 29.5 bits (63), Expect = 2.0 Identities = 28/133 (21%), Positives = 54/133 (40%), Gaps = 8/133 (6%) Query: 587 RDIAVGLYSRTVFILYDQFIEV----LSAILTWLAYLVPSYAMSGLYAPTPGAFDAFYIY 642 R+ +YS F+ +E+ + L +L + P ++ P A+ +Y Sbjct: 1284 REKPSNIYSWVAFVFSAIIVEIPFNLVFGTLFFLCWFYPIKFYKHIHHPGDKTGYAWLLY 1343 Query: 643 LGCMVLFFINIQMVCRATIFLVPMEKTASVIAGVCLLLGTLVNGVMIQQADLPYYVKWLQ 702 + + F Q V A P +TASV+ + NGV+ ++L + W+ Sbjct: 1344 MFFQMYFSTFGQAVASAC----PNAQTASVVNSLLFTFVITFNGVLQPNSNLVGFWHWMH 1399 Query: 703 YVSPSRWTIPEIL 715 ++P + I +L Sbjct: 1400 SLTPFTYLIEGLL 1412 >SPAPB24D3.09c |pdr1||ABC transporter Pdr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1396 Score = 67.3 bits (157), Expect = 8e-12 Identities = 65/261 (24%), Positives = 123/261 (47%), Gaps = 18/261 (6%) Query: 204 VNSLEITSPENKSTLVVSGLSFEVKCGEILAVLATAQHEATGLLDVLA--GVRKKLSGDI 261 +N +T K +++ +S +K + A+L + + LL +L+ G+ + G+I Sbjct: 763 LNFTVVTKTSKKQ--ILTNVSGYLKKNTLTALLGENKSGKSVLLRILSQRGIAGSVEGEI 820 Query: 262 VINGQPVASSTLRKVVAYVRNDTALCPTMSVEHTLRFHAALRKPRNTHSHVKMGDRDRIN 321 ++G + LRK + YVR + +V TLR HAALR+ + + + Sbjct: 821 TLSGLKNPKN-LRKRIGYVRKNPLFISEYTVRETLRLHAALRQSKQRSLSEQYA---YVE 876 Query: 322 LLIEELGLEQVRDTNVGRLTRS----EIRRLNVACQL-LLDMAILILDQPTKEMDIFDTF 376 +I+ LGL +V D VG + + R L++A +L +IL+LD+P +D + Sbjct: 877 YVIDFLGLGEVADFIVGDMGKGLSLYHKRLLSIAVELSARPGSILLLDEPANGLDSQSAW 936 Query: 377 FLVEYLRNWASTGRVVIMSLHPPTYEIFAMLTKVVLISA-GRTM-FSGYRRD---MLPYF 431 LV L+ A +G ++ S+ P+ I + ++++ G T+ + RD ++ YF Sbjct: 937 MLVCILQKLARSGLSILCSVSQPSSRILELFDMMLILDLNGNTVYYDTMGRDLSKLVNYF 996 Query: 432 SSIDYPCPAFKNPSDYYLDLV 452 I K+ +D+ L + Sbjct: 997 KRIHGTNEHSKDTADFVLHCI 1017 Score = 32.3 bits (70), Expect = 0.28 Identities = 34/135 (25%), Positives = 60/135 (44%), Gaps = 12/135 (8%) Query: 580 SMRKHVERDIAVGLYSRTVFILYDQFIEVLSAILTWLAYLVPSYAMSGLYA-----PTPG 634 S+ + E++ A+ YSRT FI+ +E + + Y SG Y P G Sbjct: 1149 SIYEQYEQNTAI--YSRTAFIIAFFLVEAVINCCFATLFFFGWYYPSGFYEFNHNIPFYG 1206 Query: 635 AFDAFYIYLGCMVLFFINIQMVCRATIFLVPMEKTASVIAGVCLLLGTLVNGVMIQQADL 694 F ++ L L++ + + ATI P TAS+I+G + NG++ + Sbjct: 1207 GFA--WLMLMIFTLYYTTLGIGI-ATIS--PSIGTASIISGTAFVFIQYFNGMIQLPGVI 1261 Query: 695 PYYVKWLQYVSPSRW 709 + KW+ +SP ++ Sbjct: 1262 VGFWKWMDALSPYKY 1276 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 33.9 bits (74), Expect = 0.092 Identities = 19/83 (22%), Positives = 42/83 (50%), Gaps = 1/83 (1%) Query: 312 VKMGDRDRINLLIEELGL-EQVRDTNVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEM 370 V + DRD I ++E +++ + +G L+ +L + + + IL+LD+PT + Sbjct: 520 VPIKDRDEIVKALKENSFTDELINMPIGSLSGGWKMKLALTRAMFKNPDILLLDEPTNHL 579 Query: 371 DIFDTFFLVEYLRNWASTGRVVI 393 D+ + +L +L N +++ Sbjct: 580 DVVNVAWLENFLVNQKDVSSIIV 602 >SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosaccharomyces pombe|chr 2|||Manual Length = 618 Score = 31.9 bits (69), Expect = 0.37 Identities = 22/74 (29%), Positives = 37/74 (50%), Gaps = 3/74 (4%) Query: 323 LIEELGLEQVRDTNVGRLTRSEIRRLNVACQLLLDMA-ILILDQPTKEMDIFDTFFLVEY 381 ++ + GL + T+ R ++ V L L+ IL+LD+PT +DI L + Sbjct: 495 VLGKFGLSGLHQTSEIRTLSDGLKSRVVFAALALEQPHILLLDEPTNHLDITSIDALAKA 554 Query: 382 LRNWASTGRVVIMS 395 + W TG VV++S Sbjct: 555 INVW--TGGVVLVS 566 Score = 27.9 bits (59), Expect = 6.0 Identities = 13/51 (25%), Positives = 30/51 (58%), Gaps = 3/51 (5%) Query: 347 RLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWASTGRVVIMSLH 397 R+ ++ L + ++L+LD+PT +D+ +L YL A ++++++ H Sbjct: 228 RVALSRALFIKPSLLLLDEPTNHLDLEAVVWLENYL---AKYDKILVVTSH 275 >SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pombe|chr 2|||Manual Length = 736 Score = 31.9 bits (69), Expect = 0.37 Identities = 16/46 (34%), Positives = 27/46 (58%) Query: 347 RLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWASTGRVV 392 RL++A L +L+LD+P+ +D+ FL EYL+ + + VV Sbjct: 362 RLSLARALFCQPDLLLLDEPSNMLDVPSIAFLSEYLQTYKNIVLVV 407 >SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 487 Score = 31.1 bits (67), Expect = 0.65 Identities = 38/187 (20%), Positives = 82/187 (43%), Gaps = 10/187 (5%) Query: 219 VVSGLSFEVKCGEILAVLATAQHEATGLLDVLAGVRKKL-SGDIVINGQPVASSTLRKVV 277 V+S +++ ++ GE A+ + T LL + G KL + +I G+ + T + Sbjct: 279 VLSDINWTIREGERWALTGSNGSGKTTLLAYVVGDHPKLFASNIKFFGKSIGPGTGISIF 338 Query: 278 AYVRNDTALCPTMS----VEHTLRFHAALRKPRNTHSHVKMGDRD--RINLLIEELGLEQ 331 N P + +HT F A L T + K+ + I+ ++EE L+ Sbjct: 339 DIQENIGHCSPEIHNHFPKQHTC-FEALLSAWSTTFTIPKLTETRLAAISSILEEFELKD 397 Query: 332 VRDTNVGRLTRSEIRRLNVACQLLLDMA-ILILDQPTKEMDIFDTFFLVEYLRNWASTGR 390 ++D + ++ ++R + C+ ++ +++LD+P + +D YL S + Sbjct: 398 IKDKPLSSISVG-MQRFILFCRAIVKQPRLVVLDEPFQGVDTKYVHMAHNYLNEKLSPSQ 456 Query: 391 VVIMSLH 397 +++ H Sbjct: 457 AMVIISH 463 Score = 29.5 bits (63), Expect = 2.0 Identities = 22/105 (20%), Positives = 49/105 (46%), Gaps = 2/105 (1%) Query: 329 LEQVRDTNVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWAS- 387 L + +++ L+ + RR +A +L+ +L+LD+P +D+ L L ++ Sbjct: 138 LSHLLPSSLINLSNGQSRRAMLASKLVQRPQLLLLDEPYAGLDVTSRSVLSSLLGEMSNH 197 Query: 388 TGRVVIMSLHPPTYEIFAMLTKVVLISAGRTMFSGYRRDMLPYFS 432 +++SL P +I +T V+ + + + G + +P S Sbjct: 198 CSPKIVLSLRPQD-KIPDFITHVLELKNKKITYQGPKEQYIPMTS 241 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 30.7 bits (66), Expect = 0.85 Identities = 21/65 (32%), Positives = 35/65 (53%), Gaps = 4/65 (6%) Query: 323 LIEELGL--EQVRDT--NVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFL 378 L++E L +Q DT L+ + +R+ +A LL D ILILD+PT +D + Sbjct: 550 LVDEFALTFDQSYDTPCKEASLSGGQQQRIALARALLRDTEILILDEPTSALDPITKNLV 609 Query: 379 VEYLR 383 ++ +R Sbjct: 610 MDAIR 614 >SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 593 Score = 30.3 bits (65), Expect = 1.1 Identities = 16/75 (21%), Positives = 35/75 (46%) Query: 323 LIEELGLEQVRDTNVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYL 382 +++ L+ + + VG L+ E++R +A + + D+P+ +DI + Sbjct: 203 VMDHTDLQNLLNREVGHLSGGELQRFAIAAVATQKADVYMFDEPSSYLDIKQRLKAGRVI 262 Query: 383 RNWASTGRVVIMSLH 397 R+ +T VI+ H Sbjct: 263 RSLLATTNYVIVVEH 277 >SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1057 Score = 29.9 bits (64), Expect = 1.5 Identities = 15/52 (28%), Positives = 29/52 (55%) Query: 331 QVRDTNVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYL 382 ++++ V L+ +L +A +L IL+LD+PT +D+ + +L YL Sbjct: 554 EMQENPVASLSGGWKMKLELARAMLQKADILLLDEPTNHLDVANIAWLEAYL 605 >SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosaccharomyces pombe|chr 3|||Manual Length = 822 Score = 29.1 bits (62), Expect = 2.6 Identities = 14/51 (27%), Positives = 29/51 (56%), Gaps = 1/51 (1%) Query: 347 RLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWASTGRVVIMSLH 397 R+ +A L + +++LD+PT +D+ +L EYL + G ++++ H Sbjct: 428 RIALARILFIKPTLMMLDEPTNHLDLEAVAWLEEYLTH-EMEGHTLLITCH 477 Score = 28.3 bits (60), Expect = 4.6 Identities = 16/49 (32%), Positives = 27/49 (55%) Query: 337 VGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNW 385 +G+L+ + RR+ A + IL+LD+PT +DI L + L N+ Sbjct: 710 MGQLSDGQRRRVLFAFLGMTQPHILLLDEPTNALDIDTIDALADALNNF 758 >SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 28.3 bits (60), Expect = 4.6 Identities = 14/38 (36%), Positives = 21/38 (55%) Query: 712 PEILRRELSETVLRNSISKDLRCTNKQRPYPDIIVQSP 749 P+I R EL +V + +SK L KQRP +++ P Sbjct: 598 PKISRPELLSSVFHDFLSKSLTVNPKQRPSSGELLRHP 635 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 28.3 bits (60), Expect = 4.6 Identities = 22/74 (29%), Positives = 35/74 (47%), Gaps = 4/74 (5%) Query: 9 LPRVFPPNHNGSQRSGTPKPKTSIGSGMKPVQRIHRQSRGVRETSSGYDSSD-NETTANY 67 LPR P + S +S TP T+I G+ ++ + +++ S SS +E +A+ Sbjct: 210 LPRSLPADVIESAKSETPAMNTNIEQGVTQPSLVNTEQLSHQQSISSTKSSKLDEISADN 269 Query: 68 NKYS---RKYRSDP 78 N S KY S P Sbjct: 270 NAQSAVDNKYSSPP 283 >SPBC18E5.12c |mas2|SPBC23G7.02c|mitochondrial processing peptidase complex alpha subunit Mas2|Schizosaccharomyces pombe|chr 2|||Manual Length = 494 Score = 28.3 bits (60), Expect = 4.6 Identities = 18/59 (30%), Positives = 26/59 (44%), Gaps = 2/59 (3%) Query: 679 LLGTLVNGVMIQQADLPYYVKWLQYVSPSRWTIPEILRRELSETVL--RNSISKDLRCT 735 LL V IQ+ DL +Y + Y + WT P+ L E + N++ L CT Sbjct: 144 LLAETVLAPKIQEDDLVHYRDSIIYENSELWTKPDALLGEFAHVTAFQNNTLGNCLLCT 202 >SPCC126.02c |pku70||Ku domain protein Pku70|Schizosaccharomyces pombe|chr 3|||Manual Length = 607 Score = 28.3 bits (60), Expect = 4.6 Identities = 11/34 (32%), Positives = 20/34 (58%) Query: 292 VEHTLRFHAALRKPRNTHSHVKMGDRDRINLLIE 325 ++H L AL+KP+ H H+KM + + + +E Sbjct: 219 LQHMLNMITALQKPKRAHFHLKMDLGNDVRIGVE 252 >SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 648 Score = 27.5 bits (58), Expect = 8.0 Identities = 32/141 (22%), Positives = 56/141 (39%), Gaps = 13/141 (9%) Query: 165 MSKAGSHMSVAHSRKHSTLRPGDQLDGYAHSAYIYPNYYVNSLEITSPENKSTLVVSGLS 224 M+ GS ++ + + STL D+ + + + ++ + SPE +L +G S Sbjct: 90 MTSLGSKVTSKNYNEFSTLEEEDEHNSHGVDVSAWAHFSL------SPEMLGSLSKAGFS 143 Query: 225 FEVKCGEILAVLATAQHEATGLLDVLAGVRKKLSGDIVINGQPVASSTLRKVVAYVRNDT 284 + ++ A+ + G D G SG + G P+ LR V A Sbjct: 144 KPMPIQSLVIPEASIGFDIIGKADT--G-----SGKTLAFGIPILEHCLRNVDAKYVQAL 196 Query: 285 ALCPTMSVEHTLRFHAALRKP 305 + PT + H + H L KP Sbjct: 197 VVAPTRELAHQICQHFELIKP 217 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.322 0.136 0.408 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,558,944 Number of Sequences: 5004 Number of extensions: 146275 Number of successful extensions: 437 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 416 Number of HSP's gapped (non-prelim): 24 length of query: 823 length of database: 2,362,478 effective HSP length: 79 effective length of query: 744 effective length of database: 1,967,162 effective search space: 1463568528 effective search space used: 1463568528 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 58 (27.5 bits)
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