SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000220-TA|BGIBMGA000220-PA|IPR003439|ABC transporter
related, IPR013525|ABC-2 type transporter
         (823 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo...    88   4e-18
SPAPB24D3.09c |pdr1||ABC transporter Pdr1|Schizosaccharomyces po...    67   8e-12
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch...    34   0.092
SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosa...    32   0.37 
SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pom...    32   0.37 
SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch...    31   0.65 
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo...    31   0.85 
SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr...    30   1.1  
SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces po...    30   1.5  
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc...    29   2.6  
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc...    28   4.6  
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha...    28   4.6  
SPBC18E5.12c |mas2|SPBC23G7.02c|mitochondrial processing peptida...    28   4.6  
SPCC126.02c |pku70||Ku domain protein Pku70|Schizosaccharomyces ...    28   4.6  
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy...    27   8.0  

>SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transporter
            Bfr1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1530

 Score = 88.2 bits (209), Expect = 4e-18
 Identities = 66/244 (27%), Positives = 125/244 (51%), Gaps = 11/244 (4%)

Query: 219  VVSGLSFEVKCGEILAVLATAQHEATGLLDVLAGVRKK--LSGDIVINGQPVASSTLRKV 276
            +++G+   V  G++ A++  +    T LL+VLA       ++GD+++NG+ +  ST ++ 
Sbjct: 900  LLNGVQGFVVPGKLTALMGESGAGKTTLLNVLAQRVDTGVVTGDMLVNGRGL-DSTFQRR 958

Query: 277  VAYVRNDTALCPTMSVEHTLRFHAALRKPRNTHSHVKMGDRDRINLLIE-ELGLEQVRDT 335
              YV+         +V   LRF AALR+P +     K    + +  L+E E   E +  T
Sbjct: 959  TGYVQQQDVHIGESTVREALRFSAALRQPASVPLSEKYEYVESVIKLLEMESYAEAIIGT 1018

Query: 336  NVGRLTRSEIRRLNVACQLLLDMAILI-LDQPTKEMDIFDTFFLVEYLRNWASTGRVVIM 394
                L   + +R  +  +L    A+L+ LD+PT  +D    + +V +LR  A  G+ ++ 
Sbjct: 1019 PGSGLNVEQRKRATIGVELAAKPALLLFLDEPTSGLDSQSAWSIVCFLRKLADAGQAILC 1078

Query: 395  SLHPPTYEIFAMLTKVVLI-SAGRTMFSG----YRRDMLPYFSSID-YPCPAFKNPSDYY 448
            ++H P+  +F    +++L+   G+T++ G    + + +L YF S     CP   NP++Y 
Sbjct: 1079 TIHQPSAVLFDQFDRLLLLQKGGKTVYFGDIGEHSKTLLNYFESHGAVHCPDDGNPAEYI 1138

Query: 449  LDLV 452
            LD++
Sbjct: 1139 LDVI 1142



 Score = 43.2 bits (97), Expect = 1e-04
 Identities = 36/169 (21%), Positives = 74/169 (43%), Gaps = 10/169 (5%)

Query: 288 PTMSVEHTLRFHAALRKPRNTHSHVKMGD---RDRINLLIEELGLEQVRDTNVGR----- 339
           P+++   TL F A  R P N   ++   +   R+R +L+    GL    +T VG      
Sbjct: 250 PSLTTAETLDFAAKCRTPNNRPCNLTRQEYVSRER-HLIATAFGLTHTFNTKVGNDFVRG 308

Query: 340 LTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWASTGRVV-IMSLHP 398
           ++  E +R+ ++        I   D  T+ +D    F  V  LR  A+  ++   ++ + 
Sbjct: 309 VSGGERKRVTISEGFATRPTIACWDNSTRGLDSSTAFEFVNVLRTCANELKMTSFVTAYQ 368

Query: 399 PTYEIFAMLTKVVLISAGRTMFSGYRRDMLPYFSSIDYPCPAFKNPSDY 447
            + +I+ +  ++ ++ AGR ++ G       YF  + + C   +   D+
Sbjct: 369 ASEKIYKLFDRICVLYAGRQIYYGPADKAKQYFLDMGFDCHPRETTPDF 417



 Score = 29.5 bits (63), Expect = 2.0
 Identities = 28/133 (21%), Positives = 54/133 (40%), Gaps = 8/133 (6%)

Query: 587  RDIAVGLYSRTVFILYDQFIEV----LSAILTWLAYLVPSYAMSGLYAPTPGAFDAFYIY 642
            R+    +YS   F+     +E+    +   L +L +  P      ++ P      A+ +Y
Sbjct: 1284 REKPSNIYSWVAFVFSAIIVEIPFNLVFGTLFFLCWFYPIKFYKHIHHPGDKTGYAWLLY 1343

Query: 643  LGCMVLFFINIQMVCRATIFLVPMEKTASVIAGVCLLLGTLVNGVMIQQADLPYYVKWLQ 702
            +   + F    Q V  A     P  +TASV+  +        NGV+   ++L  +  W+ 
Sbjct: 1344 MFFQMYFSTFGQAVASAC----PNAQTASVVNSLLFTFVITFNGVLQPNSNLVGFWHWMH 1399

Query: 703  YVSPSRWTIPEIL 715
             ++P  + I  +L
Sbjct: 1400 SLTPFTYLIEGLL 1412


>SPAPB24D3.09c |pdr1||ABC transporter Pdr1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1396

 Score = 67.3 bits (157), Expect = 8e-12
 Identities = 65/261 (24%), Positives = 123/261 (47%), Gaps = 18/261 (6%)

Query: 204  VNSLEITSPENKSTLVVSGLSFEVKCGEILAVLATAQHEATGLLDVLA--GVRKKLSGDI 261
            +N   +T    K   +++ +S  +K   + A+L   +   + LL +L+  G+   + G+I
Sbjct: 763  LNFTVVTKTSKKQ--ILTNVSGYLKKNTLTALLGENKSGKSVLLRILSQRGIAGSVEGEI 820

Query: 262  VINGQPVASSTLRKVVAYVRNDTALCPTMSVEHTLRFHAALRKPRNTHSHVKMGDRDRIN 321
             ++G     + LRK + YVR +       +V  TLR HAALR+ +      +      + 
Sbjct: 821  TLSGLKNPKN-LRKRIGYVRKNPLFISEYTVRETLRLHAALRQSKQRSLSEQYA---YVE 876

Query: 322  LLIEELGLEQVRDTNVGRLTRS----EIRRLNVACQL-LLDMAILILDQPTKEMDIFDTF 376
             +I+ LGL +V D  VG + +       R L++A +L     +IL+LD+P   +D    +
Sbjct: 877  YVIDFLGLGEVADFIVGDMGKGLSLYHKRLLSIAVELSARPGSILLLDEPANGLDSQSAW 936

Query: 377  FLVEYLRNWASTGRVVIMSLHPPTYEIFAMLTKVVLISA-GRTM-FSGYRRD---MLPYF 431
             LV  L+  A +G  ++ S+  P+  I  +   ++++   G T+ +    RD   ++ YF
Sbjct: 937  MLVCILQKLARSGLSILCSVSQPSSRILELFDMMLILDLNGNTVYYDTMGRDLSKLVNYF 996

Query: 432  SSIDYPCPAFKNPSDYYLDLV 452
              I       K+ +D+ L  +
Sbjct: 997  KRIHGTNEHSKDTADFVLHCI 1017



 Score = 32.3 bits (70), Expect = 0.28
 Identities = 34/135 (25%), Positives = 60/135 (44%), Gaps = 12/135 (8%)

Query: 580  SMRKHVERDIAVGLYSRTVFILYDQFIEVLSAILTWLAYLVPSYAMSGLYA-----PTPG 634
            S+ +  E++ A+  YSRT FI+    +E +        +    Y  SG Y      P  G
Sbjct: 1149 SIYEQYEQNTAI--YSRTAFIIAFFLVEAVINCCFATLFFFGWYYPSGFYEFNHNIPFYG 1206

Query: 635  AFDAFYIYLGCMVLFFINIQMVCRATIFLVPMEKTASVIAGVCLLLGTLVNGVMIQQADL 694
             F   ++ L    L++  + +   ATI   P   TAS+I+G   +     NG++     +
Sbjct: 1207 GFA--WLMLMIFTLYYTTLGIGI-ATIS--PSIGTASIISGTAFVFIQYFNGMIQLPGVI 1261

Query: 695  PYYVKWLQYVSPSRW 709
              + KW+  +SP ++
Sbjct: 1262 VGFWKWMDALSPYKY 1276


>SPCC417.08 |tef3||translation elongation factor
           eEF3|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1047

 Score = 33.9 bits (74), Expect = 0.092
 Identities = 19/83 (22%), Positives = 42/83 (50%), Gaps = 1/83 (1%)

Query: 312 VKMGDRDRINLLIEELGL-EQVRDTNVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEM 370
           V + DRD I   ++E    +++ +  +G L+     +L +   +  +  IL+LD+PT  +
Sbjct: 520 VPIKDRDEIVKALKENSFTDELINMPIGSLSGGWKMKLALTRAMFKNPDILLLDEPTNHL 579

Query: 371 DIFDTFFLVEYLRNWASTGRVVI 393
           D+ +  +L  +L N      +++
Sbjct: 580 DVVNVAWLENFLVNQKDVSSIIV 602


>SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 618

 Score = 31.9 bits (69), Expect = 0.37
 Identities = 22/74 (29%), Positives = 37/74 (50%), Gaps = 3/74 (4%)

Query: 323 LIEELGLEQVRDTNVGRLTRSEIRRLNVACQLLLDMA-ILILDQPTKEMDIFDTFFLVEY 381
           ++ + GL  +  T+  R     ++   V   L L+   IL+LD+PT  +DI     L + 
Sbjct: 495 VLGKFGLSGLHQTSEIRTLSDGLKSRVVFAALALEQPHILLLDEPTNHLDITSIDALAKA 554

Query: 382 LRNWASTGRVVIMS 395
           +  W  TG VV++S
Sbjct: 555 INVW--TGGVVLVS 566



 Score = 27.9 bits (59), Expect = 6.0
 Identities = 13/51 (25%), Positives = 30/51 (58%), Gaps = 3/51 (5%)

Query: 347 RLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWASTGRVVIMSLH 397
           R+ ++  L +  ++L+LD+PT  +D+    +L  YL   A   ++++++ H
Sbjct: 228 RVALSRALFIKPSLLLLDEPTNHLDLEAVVWLENYL---AKYDKILVVTSH 275


>SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 736

 Score = 31.9 bits (69), Expect = 0.37
 Identities = 16/46 (34%), Positives = 27/46 (58%)

Query: 347 RLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWASTGRVV 392
           RL++A  L     +L+LD+P+  +D+    FL EYL+ + +   VV
Sbjct: 362 RLSLARALFCQPDLLLLDEPSNMLDVPSIAFLSEYLQTYKNIVLVV 407


>SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 487

 Score = 31.1 bits (67), Expect = 0.65
 Identities = 38/187 (20%), Positives = 82/187 (43%), Gaps = 10/187 (5%)

Query: 219 VVSGLSFEVKCGEILAVLATAQHEATGLLDVLAGVRKKL-SGDIVINGQPVASSTLRKVV 277
           V+S +++ ++ GE  A+  +     T LL  + G   KL + +I   G+ +   T   + 
Sbjct: 279 VLSDINWTIREGERWALTGSNGSGKTTLLAYVVGDHPKLFASNIKFFGKSIGPGTGISIF 338

Query: 278 AYVRNDTALCPTMS----VEHTLRFHAALRKPRNTHSHVKMGDRD--RINLLIEELGLEQ 331
               N     P +      +HT  F A L     T +  K+ +     I+ ++EE  L+ 
Sbjct: 339 DIQENIGHCSPEIHNHFPKQHTC-FEALLSAWSTTFTIPKLTETRLAAISSILEEFELKD 397

Query: 332 VRDTNVGRLTRSEIRRLNVACQLLLDMA-ILILDQPTKEMDIFDTFFLVEYLRNWASTGR 390
           ++D  +  ++   ++R  + C+ ++    +++LD+P + +D         YL    S  +
Sbjct: 398 IKDKPLSSISVG-MQRFILFCRAIVKQPRLVVLDEPFQGVDTKYVHMAHNYLNEKLSPSQ 456

Query: 391 VVIMSLH 397
            +++  H
Sbjct: 457 AMVIISH 463



 Score = 29.5 bits (63), Expect = 2.0
 Identities = 22/105 (20%), Positives = 49/105 (46%), Gaps = 2/105 (1%)

Query: 329 LEQVRDTNVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWAS- 387
           L  +  +++  L+  + RR  +A +L+    +L+LD+P   +D+     L   L   ++ 
Sbjct: 138 LSHLLPSSLINLSNGQSRRAMLASKLVQRPQLLLLDEPYAGLDVTSRSVLSSLLGEMSNH 197

Query: 388 TGRVVIMSLHPPTYEIFAMLTKVVLISAGRTMFSGYRRDMLPYFS 432
               +++SL P   +I   +T V+ +   +  + G +   +P  S
Sbjct: 198 CSPKIVLSLRPQD-KIPDFITHVLELKNKKITYQGPKEQYIPMTS 241


>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1336

 Score = 30.7 bits (66), Expect = 0.85
 Identities = 21/65 (32%), Positives = 35/65 (53%), Gaps = 4/65 (6%)

Query: 323 LIEELGL--EQVRDT--NVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFL 378
           L++E  L  +Q  DT      L+  + +R+ +A  LL D  ILILD+PT  +D      +
Sbjct: 550 LVDEFALTFDQSYDTPCKEASLSGGQQQRIALARALLRDTEILILDEPTSALDPITKNLV 609

Query: 379 VEYLR 383
           ++ +R
Sbjct: 610 MDAIR 614


>SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 593

 Score = 30.3 bits (65), Expect = 1.1
 Identities = 16/75 (21%), Positives = 35/75 (46%)

Query: 323 LIEELGLEQVRDTNVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYL 382
           +++   L+ + +  VG L+  E++R  +A        + + D+P+  +DI         +
Sbjct: 203 VMDHTDLQNLLNREVGHLSGGELQRFAIAAVATQKADVYMFDEPSSYLDIKQRLKAGRVI 262

Query: 383 RNWASTGRVVIMSLH 397
           R+  +T   VI+  H
Sbjct: 263 RSLLATTNYVIVVEH 277


>SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1057

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 15/52 (28%), Positives = 29/52 (55%)

Query: 331 QVRDTNVGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYL 382
           ++++  V  L+     +L +A  +L    IL+LD+PT  +D+ +  +L  YL
Sbjct: 554 EMQENPVASLSGGWKMKLELARAMLQKADILLLDEPTNHLDVANIAWLEAYL 605


>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 822

 Score = 29.1 bits (62), Expect = 2.6
 Identities = 14/51 (27%), Positives = 29/51 (56%), Gaps = 1/51 (1%)

Query: 347 RLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNWASTGRVVIMSLH 397
           R+ +A  L +   +++LD+PT  +D+    +L EYL +    G  ++++ H
Sbjct: 428 RIALARILFIKPTLMMLDEPTNHLDLEAVAWLEEYLTH-EMEGHTLLITCH 477



 Score = 28.3 bits (60), Expect = 4.6
 Identities = 16/49 (32%), Positives = 27/49 (55%)

Query: 337 VGRLTRSEIRRLNVACQLLLDMAILILDQPTKEMDIFDTFFLVEYLRNW 385
           +G+L+  + RR+  A   +    IL+LD+PT  +DI     L + L N+
Sbjct: 710 MGQLSDGQRRRVLFAFLGMTQPHILLLDEPTNALDIDTIDALADALNNF 758


>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
           Shk1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 658

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 14/38 (36%), Positives = 21/38 (55%)

Query: 712 PEILRRELSETVLRNSISKDLRCTNKQRPYPDIIVQSP 749
           P+I R EL  +V  + +SK L    KQRP    +++ P
Sbjct: 598 PKISRPELLSSVFHDFLSKSLTVNPKQRPSSGELLRHP 635


>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
           Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 884

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 22/74 (29%), Positives = 35/74 (47%), Gaps = 4/74 (5%)

Query: 9   LPRVFPPNHNGSQRSGTPKPKTSIGSGMKPVQRIHRQSRGVRETSSGYDSSD-NETTANY 67
           LPR  P +   S +S TP   T+I  G+     ++ +    +++ S   SS  +E +A+ 
Sbjct: 210 LPRSLPADVIESAKSETPAMNTNIEQGVTQPSLVNTEQLSHQQSISSTKSSKLDEISADN 269

Query: 68  NKYS---RKYRSDP 78
           N  S    KY S P
Sbjct: 270 NAQSAVDNKYSSPP 283


>SPBC18E5.12c |mas2|SPBC23G7.02c|mitochondrial processing peptidase
           complex alpha subunit Mas2|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 494

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 18/59 (30%), Positives = 26/59 (44%), Gaps = 2/59 (3%)

Query: 679 LLGTLVNGVMIQQADLPYYVKWLQYVSPSRWTIPEILRRELSETVL--RNSISKDLRCT 735
           LL   V    IQ+ DL +Y   + Y +   WT P+ L  E +       N++   L CT
Sbjct: 144 LLAETVLAPKIQEDDLVHYRDSIIYENSELWTKPDALLGEFAHVTAFQNNTLGNCLLCT 202


>SPCC126.02c |pku70||Ku domain protein Pku70|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 607

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 11/34 (32%), Positives = 20/34 (58%)

Query: 292 VEHTLRFHAALRKPRNTHSHVKMGDRDRINLLIE 325
           ++H L    AL+KP+  H H+KM   + + + +E
Sbjct: 219 LQHMLNMITALQKPKRAHFHLKMDLGNDVRIGVE 252


>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 648

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 32/141 (22%), Positives = 56/141 (39%), Gaps = 13/141 (9%)

Query: 165 MSKAGSHMSVAHSRKHSTLRPGDQLDGYAHSAYIYPNYYVNSLEITSPENKSTLVVSGLS 224
           M+  GS ++  +  + STL   D+ + +      + ++ +      SPE   +L  +G S
Sbjct: 90  MTSLGSKVTSKNYNEFSTLEEEDEHNSHGVDVSAWAHFSL------SPEMLGSLSKAGFS 143

Query: 225 FEVKCGEILAVLATAQHEATGLLDVLAGVRKKLSGDIVINGQPVASSTLRKVVAYVRNDT 284
             +    ++   A+   +  G  D   G     SG  +  G P+    LR V A      
Sbjct: 144 KPMPIQSLVIPEASIGFDIIGKADT--G-----SGKTLAFGIPILEHCLRNVDAKYVQAL 196

Query: 285 ALCPTMSVEHTLRFHAALRKP 305
            + PT  + H +  H  L KP
Sbjct: 197 VVAPTRELAHQICQHFELIKP 217


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.322    0.136    0.408 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,558,944
Number of Sequences: 5004
Number of extensions: 146275
Number of successful extensions: 437
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 416
Number of HSP's gapped (non-prelim): 24
length of query: 823
length of database: 2,362,478
effective HSP length: 79
effective length of query: 744
effective length of database: 1,967,162
effective search space: 1463568528
effective search space used: 1463568528
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 58 (27.5 bits)

- SilkBase 1999-2023 -