Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000201-TA|BGIBMGA000201-PA|IPR001353|20S proteasome, A
and B subunits
(232 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 189 3e-49
SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyce... 89 4e-19
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 66 5e-12
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 48 2e-06
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 48 2e-06
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 42 6e-05
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 31 0.14
SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory... 29 0.76
SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 28 1.3
SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 26 4.1
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 26 4.1
SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 25 7.1
SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizo... 25 9.4
SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2 ... 25 9.4
SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 25 9.4
SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|ch... 25 9.4
SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces pombe... 25 9.4
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 189 bits (460), Expect = 3e-49
Identities = 93/223 (41%), Positives = 134/223 (60%), Gaps = 8/223 (3%)
Query: 18 QHRFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCW 77
Q +F+PY NGG+ VAIAGD +A++ DTR G++I TR Q ++ ++ + V+GA+G
Sbjct: 3 QSQFDPYVQNGGTTVAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFE 62
Query: 78 CDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGK 137
D L L + +Q R+ +Y H + M+ + A M+ T+LY KRFFPYYV +AG+D +GK
Sbjct: 63 ADALALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYYVYTTVAGIDKEGK 122
Query: 138 GCVYSYDPIGHCARHNFRAGGSAAAQLQPLLDNQIGLKNM--------QNVTEAPLPREK 189
G +YS+DP+G R RAGGSAA + P LDNQ+ L N + L E+
Sbjct: 123 GEIYSFDPVGSYEREWCRAGGSAANFITPFLDNQVNLHNQYVPGSHGKERKPRRLLKLEE 182
Query: 190 ALALLKDVFISAAERDIYTGDSIYILIITASGIQEEKFELRKD 232
A+ + D F SA ER I GDS+ + IIT G++ L+KD
Sbjct: 183 AMKITTDAFTSAGERHIEVGDSVLVKIITKEGVETRIIPLKKD 225
>SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 204
Score = 89.4 bits (212), Expect = 4e-19
Identities = 59/208 (28%), Positives = 98/208 (47%), Gaps = 12/208 (5%)
Query: 27 NGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRL 86
NGGS VA+AG + I +D RL T + K+F + + T LG TG D TL L
Sbjct: 7 NGGSCVAMAGKNCVAIASDLRLGVQSISLTNNFPKVFAMGDKTYLGLTGLATDVQTLYEL 66
Query: 87 LQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPI 146
+ ++ +Y+ + + A ++S+ LY KRF PY+ V+AG+ +D + +D I
Sbjct: 67 FRYKVNLYKFREERQIQPKTFANLVSSTLYEKRFGPYFSFPVVAGVSNDNTPFICGFDSI 126
Query: 147 GHCA--RHNFRAGGSAAAQLQPLLDNQIGLKNMQNVTEAPLPREKALALLKDVFISAAER 204
G C +F G+A QL + ++V E L + + ++A +R
Sbjct: 127 G-CIDFAEDFIVSGTATEQLYGM---------CESVYEPNLEPDDLFETISQALLNAQDR 176
Query: 205 DIYTGDSIYILIITASGIQEEKFELRKD 232
D +G + +ITA + + R+D
Sbjct: 177 DCISGWGCVVYVITADKCIKRLVKGRQD 204
>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 65.7 bits (153), Expect = 5e-12
Identities = 53/202 (26%), Positives = 91/202 (45%), Gaps = 10/202 (4%)
Query: 28 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRLL 87
G +I A+ D ++ AD+R + G I R KL +L+++ +G DT T+ LL
Sbjct: 24 GTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVADLL 83
Query: 88 QARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPIG 147
+ + MY + + A + S M Y + ++AG D G VYS G
Sbjct: 84 KYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNM-LSAGLIVAGYDEKTGGDVYSIPLGG 142
Query: 148 HCARHNFRAGGSAAAQLQPLLDNQIGLKNMQNVTEAPLPREKALALLKDVFISAAERDIY 207
+ GGS +A + D +N+T+ E+A+ LK+ A ERD
Sbjct: 143 SLHKQPLAIGGSGSAFIYGFCDANF----RENMTQ-----EEAVEFLKNAVALAMERDGS 193
Query: 208 TGDSIYILIITASGIQEEKFEL 229
+G +I ++I+ G++ + F +
Sbjct: 194 SGGTIRMVILNKDGMERKFFAI 215
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 47.6 bits (108), Expect = 2e-06
Identities = 32/114 (28%), Positives = 61/114 (53%), Gaps = 5/114 (4%)
Query: 28 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRLL 87
G S++A+ D +I AD S G D ++L K+ ++T++GA G D + RLL
Sbjct: 43 GSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQIQRLL 102
Query: 88 QARMQMYEHEHNK--SMTTPAVAQMLSTMLYYKR--FFPYYVSNVLAGLDSDGK 137
+ ++++ E + ++ + + LS +LY +R PY+ ++AG+D + K
Sbjct: 103 E-KLEIKEGNYGDGYALQPSYIHEYLSKVLYARRNKLDPYWNQLIVAGVDGENK 155
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 47.6 bits (108), Expect = 2e-06
Identities = 42/191 (21%), Positives = 86/191 (45%), Gaps = 13/191 (6%)
Query: 28 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRLL 87
G +IV + D V+GADTR + G I ++ KKL +S + G DT +T ++
Sbjct: 35 GTTIVGVIAKDCIVLGADTRATAGPIIADKNCKKLHLISPNIWCAGAGTAADTEFVTSMI 94
Query: 88 QARMQMYE-HEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPI 146
+ ++++ + + K A+ + + Y+ Y+ VL G D G +++
Sbjct: 95 SSNIELHSLYTNRKPRVVTALTMLKQHLFRYQGHIGAYL--VLGGYDCKGPH-LFTIAAH 151
Query: 147 GHCARHNFRAGGSAAAQLQPLLDNQIGLKNMQNVTEAPLPREKALALLKDVFISAAERDI 206
G + + A GS + +L+ + + L R +A+ L+K+ + D+
Sbjct: 152 GSSDKLPYVALGSGSLAAISVLETKY---------QPDLERHEAMELVKEAIEAGIFNDL 202
Query: 207 YTGDSIYILII 217
+G + +++I
Sbjct: 203 GSGSNCDLVVI 213
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 42.3 bits (95), Expect = 6e-05
Identities = 41/196 (20%), Positives = 89/196 (45%), Gaps = 10/196 (5%)
Query: 26 DNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTR 85
++G + +A V+ D+R S G I ++ KK+ +++ + G D
Sbjct: 59 NHGTTTLAFRYQHGIVVCVDSRASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWET 118
Query: 86 LLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDP 145
+L ++++ + + ++ A +++LS + Y + + + +LAG G +Y D
Sbjct: 119 VLGMECRLHQLRNKELISVSAASKILSNITYSYKGYGLSMGTMLAGTGKGGT-ALYYIDS 177
Query: 146 IGHCARHNFRAGGSAAAQLQPLLDNQIGLKNMQNVTEAPLPREKALALLKDVFISAAERD 205
G + + + GS + +LD+ G + L +++AL L + ++A RD
Sbjct: 178 DGTRLKGDLFSVGSGSTFAYGVLDS--GYR-------WDLSKQEALYLAQRSIVAATHRD 228
Query: 206 IYTGDSIYILIITASG 221
Y+G S+ + I +G
Sbjct: 229 AYSGGSVNLYHIDENG 244
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 31.1 bits (67), Expect = 0.14
Identities = 33/175 (18%), Positives = 68/175 (38%), Gaps = 6/175 (3%)
Query: 26 DNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTR 85
++ G + I D V+ A+ ++++ +KL+ + ++ + G D L
Sbjct: 29 NHAGVALGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILIN 88
Query: 86 LLQARMQMYEHEHNKSMTTPAVAQMLSTM----LYYKRFFPYYVSNVLAGLDSDGKGCVY 141
+ Q Y N+ M + + + + Y P+ VS + AG D ++
Sbjct: 89 YARRVGQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLF 148
Query: 142 SYDPIGHCARHNFRAGGSAAAQLQPLLDNQIGLKNMQNVTEAPLPREKALALLKD 196
+P G+ + G + +Q L+ + K+ N+ EA K L+ D
Sbjct: 149 QSNPSGNYGSWQANSIGGNSTSVQSLMRQE--YKDDINLDEASAMAVKFLSKTLD 201
>SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory
factor |Schizosaccharomyces pombe|chr 2|||Manual
Length = 633
Score = 28.7 bits (61), Expect = 0.76
Identities = 15/49 (30%), Positives = 29/49 (59%), Gaps = 3/49 (6%)
Query: 155 RAGGSAAAQLQPLLDNQIGLKNMQNVTEAPLPREKALALLKDVFISAAE 203
+AG S A + LL+NQI +N+T + P + ++K++ ++A+E
Sbjct: 540 KAGHSEAIERLALLENQIQEPEPENITSSQYPNQD---VIKEIPVTASE 585
>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 471
Score = 27.9 bits (59), Expect = 1.3
Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Query: 3 SVNENFPEYAVPGAKQHRFEPYADNGGSIVAIAGD-DYAVIGADT 46
S N F P K ++ +GG+ AIA D YA +G+DT
Sbjct: 118 SENNLFGRTVNPVVKDSNYDVGGSSGGAAAAIAADICYASVGSDT 162
>SPMIT.06 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 4.1
Identities = 16/56 (28%), Positives = 27/56 (48%), Gaps = 4/56 (7%)
Query: 143 YDPIGHCARHNFRAGGSAAAQLQPLLDNQIG----LKNMQNVTEAPLPREKALALL 194
Y+P+ + A H FR G S + L+ + N G ++ +P +K +ALL
Sbjct: 332 YEPLFNTASHGFRPGRSCHSALRSIFTNFKGCTWWIEGDIKACFDSIPHDKLIALL 387
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 26.2 bits (55), Expect = 4.1
Identities = 11/22 (50%), Positives = 14/22 (63%)
Query: 33 AIAGDDYAVIGADTRLSTGFSI 54
AI GD Y+ IG++ GFSI
Sbjct: 321 AIVGDTYSTIGSNVEALPGFSI 342
>SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase
Ino80|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1604
Score = 25.4 bits (53), Expect = 7.1
Identities = 12/30 (40%), Positives = 17/30 (56%)
Query: 90 RMQMYEHEHNKSMTTPAVAQMLSTMLYYKR 119
R Q ++NK M T A M TM+++KR
Sbjct: 666 RWQFRTIKNNKDMQTKAKRAMRETMVFWKR 695
>SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase
Ppk38|Schizosaccharomyces pombe|chr 3|||Manual
Length = 650
Score = 25.0 bits (52), Expect = 9.4
Identities = 10/28 (35%), Positives = 17/28 (60%)
Query: 186 PREKALALLKDVFISAAERDIYTGDSIY 213
PR LAL D+F S A+ + T ++++
Sbjct: 442 PRLSTLALADDMFSSTAKETVPTNEAVF 469
>SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 218
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/38 (34%), Positives = 21/38 (55%)
Query: 79 DTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLY 116
D TLT+L +A ++E N S+ V ++S ML+
Sbjct: 99 DPNTLTKLFEALRLLHEPNTNDSIDCRVVDAVISDMLH 136
>SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1008
Score = 25.0 bits (52), Expect = 9.4
Identities = 9/16 (56%), Positives = 11/16 (68%)
Query: 128 VLAGLDSDGKGCVYSY 143
VL G D DG+ C+Y Y
Sbjct: 686 VLLGYDKDGRPCLYLY 701
>SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 775
Score = 25.0 bits (52), Expect = 9.4
Identities = 14/62 (22%), Positives = 27/62 (43%)
Query: 9 PEYAVPGAKQHRFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSES 68
P A+P + D + I GDD + ++T S+Y+ + K+F+ ++
Sbjct: 346 PLQALPPLPLEKLYGSQDGFETAKKIVGDDLFKAFVPSAVTTASSLYSEETAKVFRAEQA 405
Query: 69 TV 70
V
Sbjct: 406 EV 407
>SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 377
Score = 25.0 bits (52), Expect = 9.4
Identities = 21/73 (28%), Positives = 29/73 (39%)
Query: 93 MYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPIGHCARH 152
++ E S TPAV + + P VS VL G+DS + V P+ R
Sbjct: 105 LFGREKQNSEATPAVQPPATCPMSNSNQKPAGVSEVLTGVDSKQQSYVPEGCPVATPKRG 164
Query: 153 NFRAGGSAAAQLQ 165
F G+ Q Q
Sbjct: 165 WFNWFGNNDDQKQ 177
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.319 0.135 0.395
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,012,369
Number of Sequences: 5004
Number of extensions: 39379
Number of successful extensions: 111
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 90
Number of HSP's gapped (non-prelim): 18
length of query: 232
length of database: 2,362,478
effective HSP length: 71
effective length of query: 161
effective length of database: 2,007,194
effective search space: 323158234
effective search space used: 323158234
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 52 (25.0 bits)
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