BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000198-TA|BGIBMGA000198-PA|IPR000719|Protein kinase, IPR011009|Protein kinase-like, IPR002290|Serine/threonine protein kinase (543 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 74 3e-15 AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 68 2e-13 AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 66 9e-13 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 57 4e-10 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 44 2e-06 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 44 2e-06 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 31 0.032 AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase... 27 0.40 AJ780964-1|CAG62942.2| 332|Apis mellifera putative corticotropi... 26 0.91 AY398690-1|AAR83734.1| 416|Apis mellifera major royal jelly pro... 25 2.1 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 73.7 bits (173), Expect = 3e-15 Identities = 64/225 (28%), Positives = 111/225 (49%), Gaps = 29/225 (12%) Query: 18 LGKGATGAVFQGVNKNNGEPVAVKTFNQLSHMRPHDVQMREFEVLKKVKHENIVKLLAIE 77 LG G G V++ + K GE VA K + + + + +KH NIVK+L IE Sbjct: 73 LGSGGFGIVYKALYK--GEQVAAK----IIQTEKYSNMLNSEKHASFLKHSNIVKVLMIE 126 Query: 78 EEQEGRGKVIVMELCTGGSLFNILDDPENTYGLQEYEFLLVLEHLTAGMKHLRDNNLVHR 137 Q +I MELC G +L N LD+ L + E + +L+ +T ++ + +VH Sbjct: 127 --QGASLSLITMELC-GTTLQNRLDEAI----LIKNERICILKSITCALQFCHNAGIVHA 179 Query: 138 DLKPGNIMKYINEDGTTTYKLTDFGAARELQEEEQFVSLYGTEEYLHPDMYERAVLRKPV 197 D+KP NI+ ++++G KLTDFG++ + + YGT Y P++ ++ +P Sbjct: 180 DVKPKNIL--MSKNGQP--KLTDFGSSVLIGAPNEIDKFYGTPGYTAPEVIKQ---NRPT 232 Query: 198 GKSFGATVDLWSIGVTLYHVATGQLPFRPYGGRRNKETMFYITTK 242 D++S+G+ + + +LPF + T+ Y++ K Sbjct: 233 -----PAADIYSLGIVAWQMLFRKLPF----AGLHSHTIIYLSAK 268 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 67.7 bits (158), Expect = 2e-13 Identities = 56/213 (26%), Positives = 96/213 (45%), Gaps = 22/213 (10%) Query: 16 SVLGKGATGAV-FQGVNKNNGEPVAVKTFN--QLSHMRPHDVQMREFEVLKKVKHENIVK 72 + LG G G V + ++ A+K Q+ R M E ++ + + +VK Sbjct: 371 ATLGVGGFGRVELVQIAGDSSRSFALKQMKKAQIVETRQQQHIMSEKRIMGEADCDFVVK 430 Query: 73 LLAIEEEQEGRGKVIVMELCTGGSLFNILDDPEN-TYGLQEYEFLLVLEHLTAGMKHLRD 131 L +++ + ++ME C GG L+ +L D + G + V+E +L Sbjct: 431 LFKTFKDR--KYLYMLMEACLGGELWTVLRDKGHFDDGTTRFYTACVVE----AFDYLHS 484 Query: 132 NNLVHRDLKPGNIMKYINEDGTTTYKLTDFGAARELQEEEQFVSLYGTEEYLHPDMYERA 191 N+++RDLKP N++ D KL DFG A+ L + + GT EY+ P++ Sbjct: 485 RNIIYRDLKPENLLL----DSQGYVKLVDFGFAKRLDHGRKTWTFCGTPEYVAPEVI--- 537 Query: 192 VLRKPVGKSFGATVDLWSIGVTLYHVATGQLPF 224 + K + D WS+GV ++ + TG PF Sbjct: 538 -----LNKGHDISADYWSLGVLMFELLTGTPPF 565 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 65.7 bits (153), Expect = 9e-13 Identities = 40/107 (37%), Positives = 56/107 (52%), Gaps = 10/107 (9%) Query: 119 LEHLTAGMKHLRDNNLVHRDLKPGNIMKYINEDGTTTYKLTDFGAARELQEEEQ-FVSLY 177 ++ + + H N +VHRDLKP N++ G KL DFG A E+Q E Q + Sbjct: 15 IQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAV-KLADFGLAIEVQGEAQAWFGFA 73 Query: 178 GTEEYLHPDMYERAVLRKPVGKSFGATVDLWSIGVTLYHVATGQLPF 224 GT YL P+ VL+K + +G VD+W+ GV LY + G PF Sbjct: 74 GTPGYLSPE-----VLKK---EPYGKPVDIWACGVILYILLVGYPPF 112 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 56.8 bits (131), Expect = 4e-10 Identities = 54/216 (25%), Positives = 101/216 (46%), Gaps = 20/216 (9%) Query: 16 SVLGKGATGAVFQGVNK--NNGEP---VAVKTFNQLSHMRPHDVQMREFEVLKKVKHENI 70 +++G G G V +G K +G VA+KT S + + + E ++ + +H N+ Sbjct: 637 AIIGGGEFGDVCRGKLKLPPDGRTEIDVAIKTLKPGSADKARNDFLTEASIMGQFEHPNV 696 Query: 71 VKLLAIEEEQEGRGKVIVMELCTGGSLFNILDDPENTYGLQEYEFLLVLEHLTAGMKHLR 130 + L + + +I+ E GSL L + + Q + + +L + +GM++L Sbjct: 697 IFLQGVVTKSNP--VMIITEFMENGSLDTFLRANDGKF--QVLQLVGMLRGIASGMQYLA 752 Query: 131 DNNLVHRDLKPGNIMKYINEDGTTTYKLTDFGAARELQE-EEQFVSLYGTEEYLHPDMYE 189 + N VHRDL N++ +N K+ DFG +RE++ E + G + + E Sbjct: 753 EMNYVHRDLAARNVL--VN--AALVCKIADFGLSREIESATEGAYTTRGGKIPVRWTAPE 808 Query: 190 RAVLRKPVGKSFGATVDLWSIGVTLYHVAT-GQLPF 224 RK F + D+WS+G+ + V + G+ P+ Sbjct: 809 AIAFRK-----FTSASDVWSMGIVCWEVMSYGERPY 839 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 44.4 bits (100), Expect = 2e-06 Identities = 60/242 (24%), Positives = 107/242 (44%), Gaps = 35/242 (14%) Query: 18 LGKGATGAVF--QGVNKNNGEPVAVKTFNQLSHMRPHDVQMREFEVLKKVKHENIVKLL- 74 +G+G G VF G G P A+K+ +D+ M + H+ IVKL Sbjct: 600 IGRGQYGIVFACDGWGGKAG-PCAIKSVVPSDESHWNDLAMEFYYNRSIPDHKRIVKLRG 658 Query: 75 AIEEEQEGRGKVIVMELCTGGSLFNILDD-PENTY-----GLQEYEFLLVLEHLTAGMKH 128 +I + G G G ++ I D + Y GL E + + + G+++ Sbjct: 659 SIIDHSYGGG------FGFGSAVLLISDRLSRDLYCGIRAGLSWLERIQIALDVLEGIRY 712 Query: 129 LRDNNLVHRDLKPGNIMKYINEDGTTTYKLTDFGAARELQEEEQFVSLYGTEEYLHPDMY 188 L LVHRD+K N++ I KLTDFG + E S+ GT ++ P++ Sbjct: 713 LHSQGLVHRDVKLKNVLLDIENRA----KLTDFGFC--ITEVMMLGSIVGTPVHMAPEL- 765 Query: 189 ERAVLRKPVGKSFGATVDLWSIGVTLYHVATG--QLPFRPYGGRRNKETMFYITTKKASG 246 + + ++VD+++ G+ +++ G +LP+ + NKE + + + KKA Sbjct: 766 --------LSGHYDSSVDVYAFGILFWYLCAGHVRLPY-TFEQFHNKE-LLWTSVKKALM 815 Query: 247 VI 248 ++ Sbjct: 816 IV 817 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 44.4 bits (100), Expect = 2e-06 Identities = 60/242 (24%), Positives = 107/242 (44%), Gaps = 35/242 (14%) Query: 18 LGKGATGAVF--QGVNKNNGEPVAVKTFNQLSHMRPHDVQMREFEVLKKVKHENIVKLL- 74 +G+G G VF G G P A+K+ +D+ M + H+ IVKL Sbjct: 638 IGRGQYGIVFACDGWGGKAG-PCAIKSVVPSDESHWNDLAMEFYYNRSIPDHKRIVKLRG 696 Query: 75 AIEEEQEGRGKVIVMELCTGGSLFNILDD-PENTY-----GLQEYEFLLVLEHLTAGMKH 128 +I + G G G ++ I D + Y GL E + + + G+++ Sbjct: 697 SIIDHSYGGG------FGFGSAVLLISDRLSRDLYCGIRAGLSWLERIQIALDVLEGIRY 750 Query: 129 LRDNNLVHRDLKPGNIMKYINEDGTTTYKLTDFGAARELQEEEQFVSLYGTEEYLHPDMY 188 L LVHRD+K N++ I KLTDFG + E S+ GT ++ P++ Sbjct: 751 LHSQGLVHRDVKLKNVLLDIENRA----KLTDFGFC--ITEVMMLGSIVGTPVHMAPEL- 803 Query: 189 ERAVLRKPVGKSFGATVDLWSIGVTLYHVATG--QLPFRPYGGRRNKETMFYITTKKASG 246 + + ++VD+++ G+ +++ G +LP+ + NKE + + + KKA Sbjct: 804 --------LSGHYDSSVDVYAFGILFWYLCAGHVRLPY-TFEQFHNKE-LLWTSVKKALM 853 Query: 247 VI 248 ++ Sbjct: 854 IV 855 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 30.7 bits (66), Expect = 0.032 Identities = 22/79 (27%), Positives = 37/79 (46%), Gaps = 7/79 (8%) Query: 88 VMELCTGGSLFNILDDPENTYGLQEYEFLLVLEHLTAGMKHLRDNNLVHRDLKPGNIMKY 147 VME GG L + + +E + + G+ L +V+RDLK N++ Sbjct: 63 VMEYVNGGDLMYQI---QQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVL-- 117 Query: 148 INEDGTTTYKLTDFGAARE 166 +++DG K+ DFG +E Sbjct: 118 LDQDG--HIKIADFGMCKE 134 >AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase protein. Length = 1143 Score = 27.1 bits (57), Expect = 0.40 Identities = 13/33 (39%), Positives = 18/33 (54%) Query: 376 DTSADEPLLLFNKNNNNVSMPAEPDPPKFPVFP 408 + S EPL +F ++ N MP+EP P V P Sbjct: 941 EISDGEPLYVFVRSAPNFYMPSEPKAPMILVGP 973 >AJ780964-1|CAG62942.2| 332|Apis mellifera putative corticotropin releasing hormone-binding protein protein. Length = 332 Score = 25.8 bits (54), Expect = 0.91 Identities = 12/30 (40%), Positives = 16/30 (53%) Query: 245 SGVISGTQTTENGPIDWARELPPHCQLSVG 274 S +S T+T E G I E PH Q+ +G Sbjct: 237 SNFLSSTRTAETGTIRKCDESSPHDQVIIG 266 >AY398690-1|AAR83734.1| 416|Apis mellifera major royal jelly protein 8 protein. Length = 416 Score = 24.6 bits (51), Expect = 2.1 Identities = 16/61 (26%), Positives = 28/61 (45%), Gaps = 2/61 (3%) Query: 5 RGSENYVWSTTSVLGKGATGAVFQGVNKNNGEPVAVKTFNQLSHMRPHDVQMREFEVLKK 64 +G EN +W+ S G G +F G+ + +A N+L R +V + E L+ Sbjct: 294 QGKENILWTQASAKGISDNGVLFFGLVGDTS--LACWNENRLLDRRNIEVVAKNKETLQA 351 Query: 65 V 65 + Sbjct: 352 I 352 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.315 0.132 0.379 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 155,645 Number of Sequences: 429 Number of extensions: 6966 Number of successful extensions: 32 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 14 Number of HSP's gapped (non-prelim): 11 length of query: 543 length of database: 140,377 effective HSP length: 61 effective length of query: 482 effective length of database: 114,208 effective search space: 55048256 effective search space used: 55048256 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 46 (22.6 bits)
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