Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000189-TA|BGIBMGA000189-PA|IPR000357|HEAT
(680 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 193 6e-50
SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 33 0.098
SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pomb... 32 0.30
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 32 0.30
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 29 2.1
SPBC1709.13c |||lysine methyltransferase |Schizosaccharomyces po... 28 3.7
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 28 3.7
SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 28 3.7
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 27 6.5
SPAC3G9.05 |||GTPase activating protein |Schizosaccharomyces pom... 27 6.5
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 27 8.5
>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
metabolism|Schizosaccharomyces pombe|chr 2|||Manual
Length = 811
Score = 193 bits (471), Expect = 6e-50
Identities = 133/468 (28%), Positives = 223/468 (47%), Gaps = 18/468 (3%)
Query: 202 PDLLDGLFK--MLDDPNPEIRRMCDVQLNEFLRSIKKEPAKVDFQAMINILIIHAQSPEE 259
PD D + + M D EI + L + I + ++D++ ++ I+I H S
Sbjct: 295 PDFFDSMVRRNMSDAELKEISDYVESSLRDG-SFILEAHIQIDYKRILEIIIDHLGSSVP 353
Query: 260 LLQLTAITWLKEFVELAGAAMLPFASGILCAVLPCHAYSDEPRKKRTGIRETAATVNFQL 319
L+Q A+ WL EF+ +A +L +L +LP + + R+ + + N +
Sbjct: 354 LIQEKALKWLFEFIYIAPKDVLLQIPKVLENLLPLMSNDENMRQSAKDLSQ-----NLVI 408
Query: 320 IKXXXXXXXXXXXXXXXXXXXXXXXXXXVVGVLTQMLHHSSVHTKVAALDWILHLYNKLP 379
+ ++ VL ++L + + T++ AL+W+L L +
Sbjct: 409 LVSKIMDIEFSGSETNNKDNSLSVDFRSLIEVLQKLLSNDNEETRLCALEWVLLLQRRTG 468
Query: 380 KQMCGETERVWAGAVGSLTDAAHDVLRRALAVLAEICSAAGDLQSSPYYYKFLQAXXXXX 439
++ + ++ + L+D + V+ R L +LA I + + P+ LQ
Sbjct: 469 GKLINMHDPIFQTLLLQLSDPSDLVVSRTLELLAHIAISHKSVNLVPFLKSLLQMFAEDR 528
Query: 440 XXXXXXXXXXGAFIIRQLCVLLNAEDIYRALANILQQEKNLRFVTTMVDILNTILLTSAE 499
G IIRQLC + E +Y + A IL+ E+NL + MV++LN L T+ E
Sbjct: 529 KFLNSR----GNLIIRQLCNYIEGERVYTSFAGILETEENLELASIMVEVLNNNLFTAPE 584
Query: 500 LYELRALLKDRSKPWSRSLFLALHGCWCHSPVSLLALCLLAHHYRHCNTLIATFGDLEIT 559
LY+LR LK +S P +++F L+ WCH+ +++ +LCLL+ +Y H L++ F ++E
Sbjct: 585 LYDLRKKLK-QSAPKLQNIFTTLYTAWCHNSIAVFSLCLLSQNYEHAANLLSVFAEIEFN 643
Query: 560 VEFLTEVDKLVQLIESPIFAYLRLELV-GEHSXXXXXXXXXXXXXXPQSDAFHALRARLH 618
++ L ++DKLVQLIESP+F Y+RL+L+ E PQS AF LR RL
Sbjct: 644 IDMLIQLDKLVQLIESPVFTYMRLQLLEPEKYPYLHKALYGILMLLPQSSAFRTLRDRLQ 703
Query: 619 CLPPPHQPHRPRGDEREGSEAADDEPLDYGALLAQFHAVQAQHR-QYR 665
C P + + +ER DD P + LL + AVQ H+ YR
Sbjct: 704 CSSTP-RTNTILANERLPRSRRDD-PY-WTDLLERLKAVQLSHQNNYR 748
Score = 116 bits (280), Expect = 8e-27
Identities = 64/153 (41%), Positives = 92/153 (60%), Gaps = 14/153 (9%)
Query: 14 VRGLCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSST--NPNMKNGA 71
VRGL KLY+KRK E+E++VK + + T +I+ +I L D + S PN G
Sbjct: 6 VRGLTHKLYDKRKATAYELERVVKGYLENDETEKIRAVISQLANDFVYSPARGPNATFGG 65
Query: 72 LMGLSTVAVGLGKASVDYLPELTNPIVACFSESESRVRYQAAEALFN------------F 119
L+GL+ VA+ LG Y+ + P++ CF++S+S++RY A E+++N F
Sbjct: 66 LIGLAAVAIALGPKIDSYMESILLPVLYCFNDSDSKIRYYACESMYNIGKVAKGEVFRYF 125
Query: 120 PVIFDALAKLAADPEQQVKHAAELLDRLLKDIV 152
+IFD L KL AD E VK+ AELLDRL+KDIV
Sbjct: 126 NLIFDVLCKLFADTEITVKNGAELLDRLIKDIV 158
Score = 68.1 bits (159), Expect = 4e-12
Identities = 34/78 (43%), Positives = 48/78 (61%), Gaps = 1/78 (1%)
Query: 169 ERMYTRSAPARQLAVSWVAVLDAVPDLNVLAHLPDLLDGLFKMLDDPNPEIRRMCDVQLN 228
ER+Y + R VSW+ +LD++PDL +++LP LLDGL L DPN IR + L
Sbjct: 211 ERLYVINPNTRMFLVSWIRLLDSIPDLEFISYLPFLLDGLMNYLSDPNESIRIVTSNCLY 270
Query: 229 EFLRSIKKEPAKVDFQAM 246
+FLR I+K AKV + +
Sbjct: 271 DFLREIQK-IAKVKYHIL 287
>SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein
Sap155|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1188
Score = 33.5 bits (73), Expect = 0.098
Identities = 24/118 (20%), Positives = 55/118 (46%), Gaps = 3/118 (2%)
Query: 14 VRGLCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALM 73
V D+ RK+ ++K++ ++ ++++L+ L L + +++ ++
Sbjct: 738 VNNFKDESEPYRKMTAETVDKVIGSLGVSEIDERLEELL--LDGVLFAFQEQSVEEKVIL 795
Query: 74 G-LSTVAVGLGKASVDYLPELTNPIVACFSESESRVRYQAAEALFNFPVIFDALAKLA 130
STV LG YLP++ + I+ + + VR QAA+ + + ++ A + A
Sbjct: 796 TCFSTVVNALGTRCKPYLPQIVSTILYRLNNKSANVREQAADLVSSITIVLKACGEEA 853
>SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1095
Score = 31.9 bits (69), Expect = 0.30
Identities = 22/90 (24%), Positives = 43/90 (47%), Gaps = 8/90 (8%)
Query: 59 LMSSTNPNMKNGALMGLSTVAVGLGKASVDYLPELTNPIVACFSESESRVRYQAAEALFN 118
L+ S + ++ ALM +S++A G K L + + ++ ++ RVR+ A A+
Sbjct: 379 LIPSQKWSERHAALMAISSIAEGAEKLMKKELSRVLDMVLPLLADPHPRVRWAACNAVGQ 438
Query: 119 FPVIFDALAKLAADPEQQVKHAAELLDRLL 148
F P+ QVK+ + +L+ L+
Sbjct: 439 MSTDF--------APDMQVKYPSRILEALV 460
Score = 27.9 bits (59), Expect = 4.9
Identities = 28/98 (28%), Positives = 52/98 (53%), Gaps = 11/98 (11%)
Query: 201 LPDLLDGLFKMLDDPNPEIR-RMCDV--QLN-EFLRSIK-KEPAKVDFQAMINILIIHAQ 255
L +LD + +L DP+P +R C+ Q++ +F ++ K P+++ +A++ +L +
Sbjct: 410 LSRVLDMVLPLLADPHPRVRWAACNAVGQMSTDFAPDMQVKYPSRI-LEALVPVL----E 464
Query: 256 SPEELLQLTAITWLKEFVELAGAAML-PFASGILCAVL 292
SPE +Q A + F E A +L P+ IL +L
Sbjct: 465 SPESRVQAHAAAAMVNFSEEADNKVLEPYLDDILQRLL 502
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 31.9 bits (69), Expect = 0.30
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 5/78 (6%)
Query: 17 LCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMGLS 76
L D + + R A + +++ + K + I +L VL S + + GA GLS
Sbjct: 1650 LIDPVPDTRATAAKALGSLIEKLGEKKFPTLIPELFNVL----RSECSEVDRQGAAQGLS 1705
Query: 77 TVAVGLGKASV-DYLPEL 93
+ GLG A + D LPE+
Sbjct: 1706 EILAGLGLARLEDVLPEI 1723
Score = 31.1 bits (67), Expect = 0.52
Identities = 39/197 (19%), Positives = 80/197 (40%), Gaps = 9/197 (4%)
Query: 43 KNTSQIKKLIKVLGQDLMSSTNPNMKNGALMGLSTVAVGLG-KASVDY--LPELTNPIVA 99
K+ + K+ ++L LM+ST+ + GA GL+ + G G KA D+ L L+ I
Sbjct: 1353 KSLGKSKEYYELLSNKLMNSTSLADQKGAAYGLAGLVKGYGIKAFQDFNILDSLSELISN 1412
Query: 100 CFSESESRVRYQAAEA------LFNFPVIFDALAKLAADPEQQVKHAAELLDRLLKDIVT 153
+ + +V A EA ++ P + D L L E +K I++
Sbjct: 1413 RQNATHRQVALFAVEAFSRILGIYFEPYLPDLLPLLLTSFGDNANEVREATMDAVKQIMS 1472
Query: 154 XXXXXXXXXXXXXXRERMYTRSAPARQLAVSWVAVLDAVPDLNVLAHLPDLLDGLFKMLD 213
+ + + +++ +V + ++ + + LP ++ L ++L
Sbjct: 1473 QLSAFGVKLLLPTLLDGLNEYNWRSKKASVEILGLMSYMAPKQLSVFLPTIIPKLSEVLT 1532
Query: 214 DPNPEIRRMCDVQLNEF 230
D + ++R + L F
Sbjct: 1533 DSHSQVRNTANKSLLRF 1549
Score = 27.1 bits (57), Expect = 8.5
Identities = 41/223 (18%), Positives = 86/223 (38%), Gaps = 9/223 (4%)
Query: 36 VKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMGLSTVAVGLGKASVDYLPELTN 95
VK + +K L+ L D ++ N K ++ L ++ K +LP +
Sbjct: 1467 VKQIMSQLSAFGVKLLLPTL-LDGLNEYNWRSKKASVEILGLMSYMAPKQLSVFLPTIIP 1525
Query: 96 PIVACFSESESRVRYQAAEALFNF------PVIFDALAKLAADPEQQVKHAAELLDRLLK 149
+ ++S S+VR A ++L F P I + L ++ + L+ LLK
Sbjct: 1526 KLSEVLTDSHSQVRNTANKSLLRFGDVISNPEIQTLVPTLLKALSDCTRYTDDALEALLK 1585
Query: 150 -DIVTXXXXXXXXXXXXXXRERMYTRSAPARQLAVSWVAVLDAVPDL-NVLAHLPDLLDG 207
V + + R+A ++ + ++ ++ + N+ +L L+
Sbjct: 1586 TSFVHYLDPPSLALVIPILKYGLRERNAGTKRQSAKIFGLMASLTEPENLAVYLESLMPR 1645
Query: 208 LFKMLDDPNPEIRRMCDVQLNEFLRSIKKEPAKVDFQAMINIL 250
L ++L DP P+ R L + + ++ + N+L
Sbjct: 1646 LREVLIDPVPDTRATAAKALGSLIEKLGEKKFPTLIPELFNVL 1688
>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
Tor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2337
Score = 29.1 bits (62), Expect = 2.1
Identities = 15/54 (27%), Positives = 28/54 (51%)
Query: 197 VLAHLPDLLDGLFKMLDDPNPEIRRMCDVQLNEFLRSIKKEPAKVDFQAMINIL 250
+ AH+ + + L+ L DP IR L+EFL+ +++ + + Q +IL
Sbjct: 161 IYAHIDSIFELLWHGLRDPKVTIRIASADALSEFLKIVRQRDSSIRLQWYTSIL 214
>SPBC1709.13c |||lysine methyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 547
Score = 28.3 bits (60), Expect = 3.7
Identities = 15/43 (34%), Positives = 22/43 (51%)
Query: 223 CDVQLNEFLRSIKKEPAKVDFQAMINILIIHAQSPEELLQLTA 265
C QL+ + + K P K FQ ++ L + S EL +LTA
Sbjct: 282 CQFQLSNLVFFLPKSPDKEIFQKILQCLAVVTASSLELRKLTA 324
>SPBC30D10.10c |tor1||phosphatidylinositol kinase
Tor1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2335
Score = 28.3 bits (60), Expect = 3.7
Identities = 28/109 (25%), Positives = 44/109 (40%), Gaps = 5/109 (4%)
Query: 174 RSAPARQLAVSWVAVLDAVPDLNVLAHLPDLLDGLFKMLDDPNPEIRRMCDVQLNEFLRS 233
R R A+ + L V ++ ++ L+ L DP P IR L L
Sbjct: 139 RQEQKRMAAILIIKALAQNSPTLVYLYISEIFQNLWTGLRDPKPLIRETAADALGASLDV 198
Query: 234 IKKEPAKVDFQAMINILI--IHA--QSPEELLQLTAITWLKEFVELAGA 278
+ + AKV Q +L+ H QS E L + + + KE E +G+
Sbjct: 199 VCQREAKVQLQCFNEVLLQAEHGLRQSSVEYLHGSLLAY-KELFEKSGS 246
>SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 848
Score = 28.3 bits (60), Expect = 3.7
Identities = 22/90 (24%), Positives = 37/90 (41%), Gaps = 2/90 (2%)
Query: 465 DIYRALANILQQEKNLRFVTTMVDILNTILLTSAELYELRALLKDRSKPWSRSLFLALHG 524
D+ A ++ E+ + + DI+ L + E+ + + K R+L LAL G
Sbjct: 283 DLQSAFFSVKTAEREAALLCILQDIIKLPLKDNVRPREIGNV--NNPKKRKRALELALKG 340
Query: 525 CWCHSPVSLLALCLLAHHYRHCNTLIATFG 554
SP S L HH + + L+ G
Sbjct: 341 SESGSPDSTLVFVPTKHHVEYVSELLVQAG 370
>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1679
Score = 27.5 bits (58), Expect = 6.5
Identities = 21/73 (28%), Positives = 37/73 (50%), Gaps = 5/73 (6%)
Query: 169 ERMYTRSAPARQLAVSW-VAVLDAVPDLNVLAHLPDLLDGLFKMLDDPNPEIRR----MC 223
E M +RS R+ +V+ V++L VP L L D+ + F+ LDD +R +C
Sbjct: 1074 EFMGSRSWRDRESSVNTLVSLLSNVPVTEYLNQLEDIWNMSFRTLDDIKESVREASFPLC 1133
Query: 224 DVQLNEFLRSIKK 236
+ ++S++K
Sbjct: 1134 KLLARSVIQSLEK 1146
>SPAC3G9.05 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 659
Score = 27.5 bits (58), Expect = 6.5
Identities = 43/188 (22%), Positives = 77/188 (40%), Gaps = 15/188 (7%)
Query: 20 KLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNM-KNGALMGLSTV 78
+L EK A E E +K NDA+N IK L + S NP + KN LM V
Sbjct: 271 QLEEKLHEASHEAEISIKKLNDAENR------IKELENNPTLSFNPELEKNLKLMQELIV 324
Query: 79 AVGLGKASVDYLPELTNPIVACFSESE--SRVRYQAAEALFNFPVIFDALAKLAADPEQQ 136
A + ++ EL + I + +E+E +V A A + + L + P +
Sbjct: 325 A---ESSKTQHIVELESCIDSLKAETERWKKVAINAKSAKIDEDI---RLFTMTTVPMKS 378
Query: 137 VKHAAELLDRLLKDIVTXXXXXXXXXXXXXXRERMYTRSAPARQLAVSWVAVLDAVPDLN 196
++ L + + R+ + A+ +A++ A++ ++ + +
Sbjct: 379 IRELISPNGFLDEQLYIRYFVEMNVFVASLRRDSPSQWMSTAKDIALTLEAIVSSLREKS 438
Query: 197 VLAHLPDL 204
+L LPDL
Sbjct: 439 LLEELPDL 446
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 27.1 bits (57), Expect = 8.5
Identities = 29/102 (28%), Positives = 43/102 (42%), Gaps = 14/102 (13%)
Query: 13 CVRGLCDKLYEKRKVAGVEIEKM--VKDFNDAKNTSQIKKLIK--------VLGQDLMSS 62
CV L ++ EK+ + K+ V D N K SQ + I V +L+ S
Sbjct: 685 CVHQLLER--EKKMSDAMRQTKLTIVDDHNSRKLESQYNEFISQCSAHYENVFSLNLVIS 742
Query: 63 TNPNMKNGALMGLSTVAVGLG--KASVDYLPELTNPIVACFS 102
+ N L V +G KAS++ L + PI+ CFS
Sbjct: 743 DAMKLTNNIADALKNNKVNIGTIKASLEVLVKCIAPIIPCFS 784
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.322 0.136 0.403
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,634,399
Number of Sequences: 5004
Number of extensions: 95741
Number of successful extensions: 264
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 237
Number of HSP's gapped (non-prelim): 24
length of query: 680
length of database: 2,362,478
effective HSP length: 78
effective length of query: 602
effective length of database: 1,972,166
effective search space: 1187243932
effective search space used: 1187243932
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 57 (27.1 bits)
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