Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000146-TA|BGIBMGA000146-PA|IPR000008|C2
calcium-dependent membrane targeting, IPR002219|Protein kinase C,
phorbol ester/diacylglycerol binding, IPR008973|C2
calcium/lipid-binding region, CaLB
(534 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 35 0.025
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 34 0.043
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 34 0.043
SPBC83.18c |||C2 domain protein|Schizosaccharomyces pombe|chr 2|... 33 0.13
SPCC1322.11 |rpl2302|rpl23-2|60S ribosomal protein L23|Schizosac... 29 1.6
SPAC3G9.03 |rpl2301|rpl23-1|60S ribosomal protein L23|Schizosacc... 29 1.6
SPAC1B3.13 |||U3 snoRNP-associated protein Nan1|Schizosaccharomy... 29 2.1
SPBC1347.06c |cki1||serine/threonine protein kinase Cki1|Schizos... 27 4.9
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac... 27 6.5
SPAC922.05c |||membrane transporter |Schizosaccharomyces pombe|c... 27 6.5
SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosa... 27 8.6
SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomy... 27 8.6
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 35.1 bits (77), Expect = 0.025
Identities = 17/63 (26%), Positives = 26/63 (41%), Gaps = 3/63 (4%)
Query: 462 RRKGVKLHIYNEHTFIAKHLSGGTVCAVCDKPVARRLGKQGYECRDCRLRCHKHCHVRAA 521
R++ +H H F+ CA+C + + G Q C DC CHK C+ +
Sbjct: 394 RQRKESVHEVYGHKFLQHQFYQIMRCALCGEFLKNAAGMQ---CIDCHYTCHKKCYPKVV 450
Query: 522 AAC 524
C
Sbjct: 451 TKC 453
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 34.3 bits (75), Expect = 0.043
Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Query: 485 TVCAVCDKPVARRLGKQGYECRDCRLRCHKHCHVRAAAAC 524
T C+ C + + L K+G C++C +CH C + A C
Sbjct: 409 TSCSYC-REIIWGLSKRGCVCKNCGFKCHARCELLVPANC 447
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 34.3 bits (75), Expect = 0.043
Identities = 16/55 (29%), Positives = 25/55 (45%), Gaps = 3/55 (5%)
Query: 474 HTFIAKHLSGGTVCAVCDKPVARRLGKQGYECRDCRLRCHKHCHVRAAAACPAST 528
H F+ + CAVC + + G +C +C CHK C + A+C A +
Sbjct: 414 HQFVQRQFYQIMRCAVCAELFSY---SPGLQCENCSFVCHKKCVTKVLASCIAQS 465
>SPBC83.18c |||C2 domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 272
Score = 32.7 bits (71), Expect = 0.13
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 3/64 (4%)
Query: 29 LLVKIVGAKDLGGKT--GCIAPYCVVEMDEPPQKNQTGFKKETTSPQWDEHFLFDLSPQT 86
L+V+I AK+L K G +PYCV + E ++ QT K+ P W+ F++ ++
Sbjct: 9 LVVRIWKAKNLPNKALVGKQSPYCVCRVGEVVKRTQTD-KRSGQEPSWNAVLEFNIPSES 67
Query: 87 AELL 90
++
Sbjct: 68 YHIM 71
>SPCC1322.11 |rpl2302|rpl23-2|60S ribosomal protein
L23|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 29.1 bits (62), Expect = 1.6
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 7/85 (8%)
Query: 418 VSGFSTASARTFIHEAST--LVLETIEAG---IKKHYLVPLAIAQRSKWRRK-GVKLHIY 471
VS F T + + AS +VL T++ G ++K + + + QR WRRK GV L+ +
Sbjct: 39 VSVFGTGARLNRLPAASCGDMVLATVKKGKPDLRKKIMPAIVVRQRKAWRRKDGVYLY-F 97
Query: 472 NEHTFIAKHLSGGTVCAVCDKPVAR 496
++ + + G + PVA+
Sbjct: 98 EDNAGVIVNPKGEMKGSAITGPVAK 122
>SPAC3G9.03 |rpl2301|rpl23-1|60S ribosomal protein
L23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 139
Score = 29.1 bits (62), Expect = 1.6
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 7/85 (8%)
Query: 418 VSGFSTASARTFIHEAST--LVLETIEAG---IKKHYLVPLAIAQRSKWRRK-GVKLHIY 471
VS F T + + AS +VL T++ G ++K + + + QR WRRK GV L+ +
Sbjct: 39 VSVFGTGARLNRLPAASCGDMVLATVKKGKPDLRKKIMPAIVVRQRKAWRRKDGVYLY-F 97
Query: 472 NEHTFIAKHLSGGTVCAVCDKPVAR 496
++ + + G + PVA+
Sbjct: 98 EDNAGVIVNPKGEMKGSAITGPVAK 122
>SPAC1B3.13 |||U3 snoRNP-associated protein Nan1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 800
Score = 28.7 bits (61), Expect = 2.1
Identities = 18/74 (24%), Positives = 34/74 (45%), Gaps = 1/74 (1%)
Query: 140 EQHDVSGTLTVEFLFIDGPDIPDFGSRPVKIKESLRTVSPHGQLXXXXXXXFHQNGIPAQ 199
EQ VSG + E ++ P S+ ++ +VSP+G+L F+ + + Q
Sbjct: 337 EQIHVSGINSFESKYLTSTG-PKNTSKQLQGLVQFSSVSPNGELLLMSSSSFNGHSVSVQ 395
Query: 200 DNSKLTESALRELE 213
+ +S +R+ E
Sbjct: 396 EYDLTKDSTIRKFE 409
>SPBC1347.06c |cki1||serine/threonine protein kinase
Cki1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 27.5 bits (58), Expect = 4.9
Identities = 15/52 (28%), Positives = 25/52 (48%)
Query: 356 SRTRGQSLEMTDSELETQNRMRSVSAERNSHEKALAIPNRNVYSAGASPAQS 407
S+ LE L+ ++V ++R+ +EK A P+ N S A P Q+
Sbjct: 320 SKPPAPKLESKSPALQNHASTQNVVSKRSDYEKPFAEPHLNSASDSAEPNQN 371
>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
Rad4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 648
Score = 27.1 bits (57), Expect = 6.5
Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 4/47 (8%)
Query: 232 RFSIRNP-EWSVAILTNKKFAYKVELTDSGKFIEVERADAIDKQETE 277
RF I++ EW+V ++ A+ + SGKFI+ AIDK+E +
Sbjct: 450 RFKIQHATEWNVRVVG---VAWLWNIIQSGKFIDQVSPWAIDKKENQ 493
>SPAC922.05c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 27.1 bits (57), Expect = 6.5
Identities = 12/45 (26%), Positives = 19/45 (42%)
Query: 475 TFIAKHLSGGTVCAVCDKPVARRLGKQGYECRDCRLRCHKHCHVR 519
TF G++C + + +G GY L C+KH + R
Sbjct: 104 TFAGLGFFAGSICNLIGVKLTLAIGGTGYSVYTASLLCYKHVYNR 148
>SPAC1B9.02c |sck1||serine/threonine protein kinase
Sck1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 696
Score = 26.6 bits (56), Expect = 8.6
Identities = 8/25 (32%), Positives = 16/25 (64%)
Query: 72 PQWDEHFLFDLSPQTAELLFEVYDR 96
P WD +FD++ ++++ VYD+
Sbjct: 218 PHWDFETVFDVTKMKSQMVVSVYDK 242
>SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 348
Score = 26.6 bits (56), Expect = 8.6
Identities = 11/28 (39%), Positives = 13/28 (46%)
Query: 57 PPQKNQTGFKKETTSPQWDEHFLFDLSP 84
PP K+ K E WDE F D+ P
Sbjct: 275 PPSKDVLNSKIELGLDMWDERFATDMEP 302
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.131 0.381
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,215,438
Number of Sequences: 5004
Number of extensions: 84894
Number of successful extensions: 246
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 236
Number of HSP's gapped (non-prelim): 15
length of query: 534
length of database: 2,362,478
effective HSP length: 76
effective length of query: 458
effective length of database: 1,982,174
effective search space: 907835692
effective search space used: 907835692
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 56 (26.6 bits)
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