BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000144-TA|BGIBMGA000144-PA|IPR011009|Protein kinase-like, IPR000719|Protein kinase, IPR002290|Serine/threonine protein kinase, IPR001180|Citron-like (1136 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 78 5e-16 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 76 1e-15 AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 74 8e-15 AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 70 9e-14 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 69 2e-13 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 54 5e-09 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 54 5e-09 DQ435335-1|ABD92650.1| 135|Apis mellifera OBP18 protein. 27 0.66 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 77.8 bits (183), Expect = 5e-16 Identities = 51/170 (30%), Positives = 82/170 (48%), Gaps = 7/170 (4%) Query: 81 TELQIAYMCRETLMGLTYLHGMGKMHRDIKGANILLTECGD---VKLADFGVSAQITATI 137 +E ++ ++ L + + H G +HRD+K N+LL VKLADFG++ ++ Sbjct: 7 SEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGEA 66 Query: 138 NKRKSFIGTRYWMAPEVAAVERKGGYNQLCDIWACGITAIELAELQPPMFELHPMRVLFL 197 F GT +++PEV +K Y + DIWACG+ L PP ++ R L+ Sbjct: 67 QAWFGFAGTPGYLSPEVL---KKEPYGKPVDIWACGVILYILLVGYPPFWDEDQHR-LYA 122 Query: 198 MSKSGFKPPQLRDKERWSQLFHGFLKLALTKNPKKRPTAQKLLQHPFFLQ 247 K+G + + + + LT NP KR TA + L+HP+ Q Sbjct: 123 QIKTGSYDYPSPEWDTVTPEAKNLINQMLTVNPSKRITASEALKHPWICQ 172 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 76.2 bits (179), Expect = 1e-15 Identities = 45/143 (31%), Positives = 74/143 (51%), Gaps = 6/143 (4%) Query: 9 GNGELAAIKVIKLE---PGDDF--AIIQQEILMMKDCRHPNIVAYYGSYLRRDKLWISME 63 G EL AIK++K + DD ++++ +L + + P +V + + D+L+ ME Sbjct: 7 GTDELYAIKILKKDIIIQDDDVECTMVEKRVLALST-KPPFLVQLHSCFQTMDRLYFVME 65 Query: 64 YCGGGSLQDIYHVTGPLTELQIAYMCRETLMGLTYLHGMGKMHRDIKGANILLTECGDVK 123 Y GG L G E + E +GL +LHG G ++RD+K N+LL + G +K Sbjct: 66 YVNGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVLLDQDGHIK 125 Query: 124 LADFGVSAQITATINKRKSFIGT 146 +ADFG+ + + K+F GT Sbjct: 126 IADFGMCKEGISGDKTTKTFCGT 148 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 73.7 bits (173), Expect = 8e-15 Identities = 63/230 (27%), Positives = 103/230 (44%), Gaps = 10/230 (4%) Query: 11 GELAAIKVIKLEPGDDFAIIQQEILMMKDCRHPNIVAYYGSYLRRDKLWISMEYCGGGSL 70 GE A K+I+ E + ++ +K H NIV I+ME CG +L Sbjct: 88 GEQVAAKIIQTEKYSNMLNSEKHASFLK---HSNIVKVLMIEQGASLSLITMELCGT-TL 143 Query: 71 QDIYHVTGPLTELQIAYMCRETLMGLTYLHGMGKMHRDIKGANILLTECGDVKLADFGVS 130 Q+ + +I + + L + H G +H D+K NIL+++ G KL DFG S Sbjct: 144 QNRLDEAILIKNERICIL-KSITCALQFCHNAGIVHADVKPKNILMSKNGQPKLTDFGSS 202 Query: 131 AQITATINKRKSFIGTRYWMAPEVAAVERKGGYNQLCDIWACGITAIELAELQPPMFELH 190 I A N+ F GT + APEV R DI++ GI A ++ + P LH Sbjct: 203 VLIGAP-NEIDKFYGTPGYTAPEVIKQNRP---TPAADIYSLGIVAWQMLFRKLPFAGLH 258 Query: 191 PMRVLFLMSKSGFKPPQLRDKERWSQLFHGFLKLALTKNPKKRPTAQKLL 240 +++L +K G +P + + + K ++N +RPT +++ Sbjct: 259 SHTIIYLSAK-GHRPIDDNIDDEFKGTYKTLYKQMWSQNITERPTTNEVI 307 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 70.1 bits (164), Expect = 9e-14 Identities = 48/170 (28%), Positives = 78/170 (45%), Gaps = 6/170 (3%) Query: 31 QQEILMMKDCRHPNIVAYYGSYLRRDKLWISMEYCGGGSLQDIYHVTGPLTELQIAYMCR 90 ++ I+ DC +V + ++ R L++ ME C GG L + G + + Sbjct: 416 EKRIMGEADCDF--VVKLFKTFKDRKYLYMLMEACLGGELWTVLRDKGHFDDGTTRFYTA 473 Query: 91 ETLMGLTYLHGMGKMHRDIKGANILLTECGDVKLADFGVSAQITATINKRKSFIGTRYWM 150 + YLH ++RD+K N+LL G VKL DFG + ++ K +F GT ++ Sbjct: 474 CVVEAFDYLHSRNIIYRDLKPENLLLDSQGYVKLVDFGFAKRLDHG-RKTWTFCGTPEYV 532 Query: 151 APEVAAVERKGGYNQLCDIWACGITAIELAELQPPMFELHPMRVLFLMSK 200 APEV G++ D W+ G+ EL PP PM+ ++ K Sbjct: 533 APEVIL---NKGHDISADYWSLGVLMFELLTGTPPFTGGDPMKTYNIILK 579 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 69.3 bits (162), Expect = 2e-13 Identities = 57/227 (25%), Positives = 94/227 (41%), Gaps = 11/227 (4%) Query: 33 EILMMKDCRHPNIVAYYGSYLRRDKLWISMEYCGGGSLQDIYHVT-GPLTELQIAYMCRE 91 E +M HPN++ G + + + I E+ GSL G LQ+ M R Sbjct: 684 EASIMGQFEHPNVIFLQGVVTKSNPVMIITEFMENGSLDTFLRANDGKFQVLQLVGMLRG 743 Query: 92 TLMGLTYLHGMGKMHRDIKGANILLTECGDVKLADFGVSAQITATINKRKSFIGTRY--- 148 G+ YL M +HRD+ N+L+ K+ADFG+S +I + + G + Sbjct: 744 IASGMQYLAEMNYVHRDLAARNVLVNAALVCKIADFGLSREIESATEGAYTTRGGKIPVR 803 Query: 149 WMAPEVAAVERKGGYNQLCDIWACGITAIELAEL-QPPMFELHPMRVLFLMSKSGFKPPQ 207 W APE A + + D+W+ GI E+ + P + V+ + K P Sbjct: 804 WTAPEAIAFRK---FTSASDVWSMGIVCWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAP 860 Query: 208 LRDKERWSQLFHGFLKLALTKNP---KKRPTAQKLLQHPFFLQEMSK 251 + E QL + T P T KL++ P L+++++ Sbjct: 861 MDCPEAIYQLMLDCWQKERTHRPTFANLTQTLDKLIRSPDTLRKIAQ 907 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 54.4 bits (125), Expect = 5e-09 Identities = 57/194 (29%), Positives = 87/194 (44%), Gaps = 24/194 (12%) Query: 71 QDIY-HVTGPLTELQIAYMCRETLMGLTYLHGMGKMHRDIKGANILLTECGDVKLADFGV 129 +D+Y + L+ L+ + + L G+ YLH G +HRD+K N+LL KL DFG Sbjct: 684 RDLYCGIRAGLSWLERIQIALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGF 743 Query: 130 SAQITATINKRKSFIGTRYWMAPEVAAVERKGGYNQLCDIWACGITAIELAE---LQPPM 186 IT + S +GT MAPE+ + G Y+ D++A GI L P Sbjct: 744 C--IT-EVMMLGSIVGTPVHMAPELLS----GHYDSSVDVYAFGILFWYLCAGHVRLPYT 796 Query: 187 FE-LHPMRVLFLMSKS-----GFKPPQLR--DKERWSQLFHGFLKLALTKNPKKRPTAQK 238 FE H +L+ K G +P +L D E W ++ + P KRP Sbjct: 797 FEQFHNKELLWTSVKKALMIVGIRPERLPSFDDECWR-----LMEQCWSGEPSKRPLLGA 851 Query: 239 LLQHPFFLQEMSKR 252 ++ +Q+ +KR Sbjct: 852 IVPVLESIQQKAKR 865 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 54.4 bits (125), Expect = 5e-09 Identities = 57/194 (29%), Positives = 87/194 (44%), Gaps = 24/194 (12%) Query: 71 QDIY-HVTGPLTELQIAYMCRETLMGLTYLHGMGKMHRDIKGANILLTECGDVKLADFGV 129 +D+Y + L+ L+ + + L G+ YLH G +HRD+K N+LL KL DFG Sbjct: 722 RDLYCGIRAGLSWLERIQIALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGF 781 Query: 130 SAQITATINKRKSFIGTRYWMAPEVAAVERKGGYNQLCDIWACGITAIELAE---LQPPM 186 IT + S +GT MAPE+ + G Y+ D++A GI L P Sbjct: 782 C--IT-EVMMLGSIVGTPVHMAPELLS----GHYDSSVDVYAFGILFWYLCAGHVRLPYT 834 Query: 187 FE-LHPMRVLFLMSKS-----GFKPPQLR--DKERWSQLFHGFLKLALTKNPKKRPTAQK 238 FE H +L+ K G +P +L D E W ++ + P KRP Sbjct: 835 FEQFHNKELLWTSVKKALMIVGIRPERLPSFDDECWR-----LMEQCWSGEPSKRPLLGA 889 Query: 239 LLQHPFFLQEMSKR 252 ++ +Q+ +KR Sbjct: 890 IVPVLESIQQKAKR 903 >DQ435335-1|ABD92650.1| 135|Apis mellifera OBP18 protein. Length = 135 Score = 27.5 bits (58), Expect = 0.66 Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Query: 108 DIKGANILLTECGDVKLADFGV-SAQITATINKRKS 142 D G N L+TEC + AD V SA++ I K K+ Sbjct: 93 DENGVNKLITECSAISDADLAVKSAKLLKCIGKYKT 128 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.319 0.134 0.411 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 319,631 Number of Sequences: 429 Number of extensions: 13400 Number of successful extensions: 31 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 15 Number of HSP's gapped (non-prelim): 10 length of query: 1136 length of database: 140,377 effective HSP length: 65 effective length of query: 1071 effective length of database: 112,492 effective search space: 120478932 effective search space used: 120478932 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 49 (23.8 bits)
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