BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000136-TA|BGIBMGA000136-PA|IPR011009|Protein kinase-like, IPR000449|Ubiquitin-associated, IPR000719|Protein kinase, IPR008271|Serine/threonine protein kinase, active site, IPR002290|Serine/threonine protein kinase (652 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 153 3e-39 AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 101 1e-23 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 95 2e-21 AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 85 2e-18 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 83 5e-18 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 67 5e-13 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 67 5e-13 AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellif... 23 5.9 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 153 bits (372), Expect = 3e-39 Identities = 93/268 (34%), Positives = 140/268 (52%), Gaps = 11/268 (4%) Query: 43 LKTIGKGNFAKVKLAKHV-PTGKEVAIKIIDKTQL-NPGSLQKLFREVRIMKMLDHPNIV 100 L T+G G F +V+L + + + A+K + K Q+ Q + E RIM D +V Sbjct: 370 LATLGVGGFGRVELVQIAGDSSRSFALKQMKKAQIVETRQQQHIMSEKRIMGEADCDFVV 429 Query: 101 KLFQVIETEKTLYLVMEYASGGEVFDYLVLHGRMKEKEARAKFRQIVSAVQYCHQKRIIH 160 KLF+ + K LY++ME GGE++ L G + R +V A Y H + II+ Sbjct: 430 KLFKTFKDRKYLYMLMEACLGGELWTVLRDKGHFDDGTTRFYTACVVEAFDYLHSRNIIY 489 Query: 161 RDLKAENLLLDGEMNIKIADFGFSNEFTPGAKLDTFCGSPPYAAPELFQGKKYDGPEVDV 220 RDLK ENLLLD + +K+ DFGF+ G K TFCG+P Y APE+ K +D D Sbjct: 490 RDLKPENLLLDSQGYVKLVDFGFAKRLDHGRKTWTFCGTPEYVAPEVILNKGHD-ISADY 548 Query: 221 WSLGVILYTLVSGSLPFDGSTLRELRERVLRG--KYRIPFYMSTDCENLLKKFLVLNPAK 278 WSLGV+++ L++G+ PF G + +L+G P ++ + L+KK NPA+ Sbjct: 549 WSLGVLMFELLTGTPPFTGGDPMKTYNIILKGIDAIEFPRSITRNATALIKKLCRDNPAE 608 Query: 279 R-----ASLESIMRDKWMNTGYEEDELR 301 R + I + KW + G+ + LR Sbjct: 609 RLGYQKGGISEIQKHKWFD-GFNWEGLR 635 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 101 bits (243), Expect = 1e-23 Identities = 56/164 (34%), Positives = 90/164 (54%), Gaps = 9/164 (5%) Query: 136 EKEARAKFRQIVSAVQYCHQKRIIHRDLKAENLLLDGEMN---IKIADFGFSNEFTPGAK 192 E +A +QI+ +V +CH ++HRDLK ENLLL + +K+ADFG + E A+ Sbjct: 8 EADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGEAQ 67 Query: 193 L-DTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGSTLRELRERVLR 251 F G+P Y +PE+ + + Y G VD+W+ GVILY L+ G PF L ++ Sbjct: 68 AWFGFAGTPGYLSPEVLKKEPY-GKPVDIWACGVILYILLVGYPPFWDEDQHRLYAQIKT 126 Query: 252 GKYRIPF----YMSTDCENLLKKFLVLNPAKRASLESIMRDKWM 291 G Y P ++ + +NL+ + L +NP+KR + ++ W+ Sbjct: 127 GSYDYPSPEWDTVTPEAKNLINQMLTVNPSKRITASEALKHPWI 170 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 94.7 bits (225), Expect = 2e-21 Identities = 72/240 (30%), Positives = 117/240 (48%), Gaps = 18/240 (7%) Query: 46 IGKGNFAKVKLAK-HVPTGKEVAIKIIDKTQLNPGSLQK----LFREVRIMKMLDHPNIV 100 IG G F V K +P I + KT L PGS K E IM +HPN++ Sbjct: 639 IGGGEFGDVCRGKLKLPPDGRTEIDVAIKT-LKPGSADKARNDFLTEASIMGQFEHPNVI 697 Query: 101 KLFQVIETEKTLYLVMEYASGGEVFDYLVLH-GRMKEKEARAKFRQIVSAVQYCHQKRII 159 L V+ + ++ E+ G + +L + G+ + + R I S +QY + + Sbjct: 698 FLQGVVTKSNPVMIITEFMENGSLDTFLRANDGKFQVLQLVGMLRGIASGMQYLAEMNYV 757 Query: 160 HRDLKAENLLLDGEMNIKIADFGFSNEF---TPGAKLDTFCGSPP--YAAPELFQGKKYD 214 HRDL A N+L++ + KIADFG S E T GA T G P + APE +K+ Sbjct: 758 HRDLAARNVLVNAALVCKIADFGLSREIESATEGA-YTTRGGKIPVRWTAPEAIAFRKFT 816 Query: 215 GPEVDVWSLGVILYTLVS-GSLPFDGSTLRELRERVLRGKYRIPFYMSTDCENLLKKFLV 273 DVWS+G++ + ++S G P+ + +++ + + +G YR+P M DC + + ++ Sbjct: 817 SAS-DVWSMGIVCWEVMSYGERPYWNWSNQDVIKSIEKG-YRLPAPM--DCPEAIYQLML 872 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 84.6 bits (200), Expect = 2e-18 Identities = 54/194 (27%), Positives = 91/194 (46%), Gaps = 10/194 (5%) Query: 46 IGKGNFAKVKLAKHVPTGKEVAIKIIDKTQLNPGSLQKLFREVRIMKMLDHPNIVKLFQV 105 +G G F V A + G++VA KII + + + + L H NIVK+ + Sbjct: 73 LGSGGFGIVYKALY--KGEQVAAKIIQTEKYS-----NMLNSEKHASFLKHSNIVKVLMI 125 Query: 106 IETEKTLYLVMEYASGGEVFDYLVLHGRMKEKEARAKFRQIVSAVQYCHQKRIIHRDLKA 165 + + ME G + + + E + I A+Q+CH I+H D+K Sbjct: 126 EQGASLSLITMELC--GTTLQNRLDEAILIKNERICILKSITCALQFCHNAGIVHADVKP 183 Query: 166 ENLLLDGEMNIKIADFGFSNEFTPGAKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGV 225 +N+L+ K+ DFG S ++D F G+P Y APE+ + + P D++SLG+ Sbjct: 184 KNILMSKNGQPKLTDFGSSVLIGAPNEIDKFYGTPGYTAPEVIKQNR-PTPAADIYSLGI 242 Query: 226 ILYTLVSGSLPFDG 239 + + ++ LPF G Sbjct: 243 VAWQMLFRKLPFAG 256 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 83.4 bits (197), Expect = 5e-18 Identities = 52/148 (35%), Positives = 80/148 (54%), Gaps = 3/148 (2%) Query: 56 LAKHVPTGKEVAIKIIDKTQL-NPGSLQKLFREVRIMKMLDHPN-IVKLFQVIETEKTLY 113 LA+ T + AIKI+ K + ++ E R++ + P +V+L +T LY Sbjct: 2 LAERKGTDELYAIKILKKDIIIQDDDVECTMVEKRVLALSTKPPFLVQLHSCFQTMDRLY 61 Query: 114 LVMEYASGGEVFDYLVLHGRMKEKEARAKFRQIVSAVQYCHQKRIIHRDLKAENLLLDGE 173 VMEY +GG++ + G+ KE A +I + + H + I++RDLK +N+LLD + Sbjct: 62 FVMEYVNGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVLLDQD 121 Query: 174 MNIKIADFGFSNEFTPGAK-LDTFCGSP 200 +IKIADFG E G K TFCG+P Sbjct: 122 GHIKIADFGMCKEGISGDKTTKTFCGTP 149 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 66.9 bits (156), Expect = 5e-13 Identities = 33/88 (37%), Positives = 53/88 (60%), Gaps = 4/88 (4%) Query: 146 IVSAVQYCHQKRIIHRDLKAENLLLDGEMNIKIADFGFSNEFTPGAKLDTFCGSPPYAAP 205 ++ ++Y H + ++HRD+K +N+LLD E K+ DFGF T L + G+P + AP Sbjct: 706 VLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFC--ITEVMMLGSIVGTPVHMAP 763 Query: 206 ELFQGKKYDGPEVDVWSLGVILYTLVSG 233 EL G YD VDV++ G++ + L +G Sbjct: 764 ELLSG-HYDS-SVDVYAFGILFWYLCAG 789 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 66.9 bits (156), Expect = 5e-13 Identities = 33/88 (37%), Positives = 53/88 (60%), Gaps = 4/88 (4%) Query: 146 IVSAVQYCHQKRIIHRDLKAENLLLDGEMNIKIADFGFSNEFTPGAKLDTFCGSPPYAAP 205 ++ ++Y H + ++HRD+K +N+LLD E K+ DFGF T L + G+P + AP Sbjct: 744 VLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFC--ITEVMMLGSIVGTPVHMAP 801 Query: 206 ELFQGKKYDGPEVDVWSLGVILYTLVSG 233 EL G YD VDV++ G++ + L +G Sbjct: 802 ELLSG-HYDS-SVDVYAFGILFWYLCAG 827 >AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellifera ORF for hypotheticalprotein. ). Length = 998 Score = 23.4 bits (48), Expect = 5.9 Identities = 10/25 (40%), Positives = 16/25 (64%) Query: 413 NTIDSASIKENTARIAQMQVQSTTR 437 NT++ AS E+T ++ +QV TR Sbjct: 920 NTVNVASRMESTGKVGCIQVTDETR 944 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.319 0.134 0.396 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 142,727 Number of Sequences: 429 Number of extensions: 5282 Number of successful extensions: 24 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 7 Number of HSP's gapped (non-prelim): 8 length of query: 652 length of database: 140,377 effective HSP length: 62 effective length of query: 590 effective length of database: 113,779 effective search space: 67129610 effective search space used: 67129610 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 47 (23.0 bits)
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