BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000132-TA|BGIBMGA000132-PA|IPR000322|Glycoside hydrolase, family 31 (849 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_13633| Best HMM Match : Glyco_hydro_31 (HMM E-Value=0) 348 1e-95 SB_27817| Best HMM Match : Glyco_hydro_31 (HMM E-Value=0) 185 2e-46 SB_5524| Best HMM Match : Glyco_hydro_31 (HMM E-Value=1.7e-10) 87 4e-17 SB_28911| Best HMM Match : No HMM Matches (HMM E-Value=.) 61 3e-09 SB_13631| Best HMM Match : No HMM Matches (HMM E-Value=.) 42 0.003 SB_8811| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.42 SB_50480| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.73 SB_52973| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.7 SB_5939| Best HMM Match : BAH (HMM E-Value=7.9e-18) 32 1.7 SB_33411| Best HMM Match : 7tm_1 (HMM E-Value=0.03) 32 2.2 SB_52268| Best HMM Match : SecA_PP_bind (HMM E-Value=0.33) 31 3.9 SB_3111| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 3.9 SB_41449| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 6.8 SB_8156| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 9.0 SB_3142| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 9.0 SB_20018| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 9.0 >SB_13633| Best HMM Match : Glyco_hydro_31 (HMM E-Value=0) Length = 663 Score = 348 bits (855), Expect = 1e-95 Identities = 185/406 (45%), Positives = 249/406 (61%), Gaps = 41/406 (10%) Query: 2 NWKYAFKLSALADGTFRVELDEADPLYPRYRTQLALDGEPKADGLK-----------LIS 50 N + LS L T R +++E P+ PRY + AL EPK + I+ Sbjct: 70 NVELHLSLSTLEKNTARFKINELKPIRPRYEVKDALKTEPKLVRMNHKSINMFVSSYKIT 129 Query: 51 KDDGKVILVN-TQGHKVIITSEPLKLEFLDQNGEVAVVLNENSQLLVEPLRARR--EKVE 107 K++ + V+ + +I+T +P +L+FL N EV V +N L E RA++ E + Sbjct: 130 KEEANSMHVSFDENSNIIVTYKPFRLDFLVGN-EVVVSINTRGLLNFEHYRAKKVGEPPK 188 Query: 108 DE------------DGDGANQ---------LDDE---EGTWSETFQSHHDSKPRGNEAVS 143 +E +GD N+ ++DE EG W E F+ HHDSKPRG +V Sbjct: 189 EEPLQPAPPGPPSIEGDEPNEEQKQEEEKVVEDEKHEEGMWEENFKEHHDSKPRGPSSVG 248 Query: 144 LDVVFPDADHVYGIPEHTDGLALKTTTSGDPYRLYNLDVFEYILDSRMAIYGSVPVMYAH 203 LD+ FP +HVYGIPEH D LALK+T +PYRLYNLDVFEY LDSRMA+YGS+PVM AH Sbjct: 249 LDISFPGVEHVYGIPEHADSLALKSTIGSEPYRLYNLDVFEYELDSRMALYGSIPVMLAH 308 Query: 204 GPKRTVGVFWHNSAETWIDVVNY-GEGNVVSSLVNLVTGGQK-KRVDARFLSESGIVDMF 261 +TVGVFW N+AETW+D+ ++++SL++ ++ +VD + SESGI+D+F Sbjct: 309 SASKTVGVFWMNAAETWVDISTADARKSMLNSLMSFFKAEEEVPQVDTHWFSESGIIDVF 368 Query: 262 VLMGSTPGDAFRQYTALTGTTPLPPKFSLGYHQCRWNYMDEADVRSVDENFDVHDIPMDS 321 V+MG P D F+QY +LTG T LPP F + YHQCRWNY DE DV+SVD++FD HDIP D Sbjct: 369 VMMGPRPHDVFKQYASLTGPTSLPPLFGIAYHQCRWNYNDEEDVKSVDDSFDKHDIPYDV 428 Query: 322 IWLDIEYTNKKMYFTWDVVKFPHPAEMVANLTAKGRKMVVIVDPHI 367 +WLDIE+T+ K Y TWD VKFP+P M N+ +KGRK V P I Sbjct: 429 LWLDIEHTDGKRYMTWDKVKFPNPEAMQENIASKGRKPSVFHGPEI 474 Score = 243 bits (595), Expect = 4e-64 Identities = 117/221 (52%), Positives = 149/221 (67%), Gaps = 14/221 (6%) Query: 415 RYYS-DRYRFENFPGTSKDVHLWNDMNEPSVFNGPEVTMPKDCRHYKPPQDGLEGLAAYW 473 RY + D+ +F N P ++ ++ + +PSVF+GPE+TM KD HY W Sbjct: 440 RYMTWDKVKFPN-PEAMQE-NIASKGRKPSVFHGPEITMHKDTIHYGD-----------W 486 Query: 474 EHRHVHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTAEWSFL 533 EHR VHN YG++ +ATN GL+ R+ G RPF+L+RA FAGTQRY +WTGDN AEWS L Sbjct: 487 EHRDVHNIYGMYFHKATNLGLIQRSGGKDRPFVLSRAFFAGTQRYGPIWTGDNMAEWSHL 546 Query: 534 AASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREP 593 AS+PM LSL + G F G+DVGGFFK PE EL+ RWYQ F PF RAH+H++TKRREP Sbjct: 547 KASIPMILSLGVTGLPFAGADVGGFFKNPEPELLARWYQTGVFTPFLRAHAHLDTKRREP 606 Query: 594 WLYPAATTALIRDANRKRYALLDFWYTLFYEHTVDGLPVMR 634 WL+ +IRDA R RYALL WYTLF+ + DG P++R Sbjct: 607 WLFDDVYKNVIRDALRTRYALLPLWYTLFFHASQDGTPIIR 647 >SB_27817| Best HMM Match : Glyco_hydro_31 (HMM E-Value=0) Length = 388 Score = 185 bits (450), Expect = 2e-46 Identities = 129/388 (33%), Positives = 182/388 (46%), Gaps = 22/388 (5%) Query: 315 HDIPMDSIWLDIEYTNKKMYFTWDVVKFPHPAEMVANL-TAKGRKMVVIVDPHIKR-EPG 372 + IP D W DIEY + FT D + + V L T + + IVDP I +P Sbjct: 4 YKIPQDVQWNDIEYMKDHLDFTVDSTNWGGLGDFVKKLHTQYDQHYIPIVDPGISNTQPS 63 Query: 373 --YFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSDRYRFENFPGTS 430 Y + D +G +V +G G WPG++ YPDFFNP Y++ + F Sbjct: 64 GSYPPYSDGLAMGVFVNASNGGPIVGQVWPGNTVYPDFFNPSTQSYWTKQ--ISQFHDVV 121 Query: 431 KDVHLWNDMNEPSVF-NGPEVTMPK---DCRHYKPPQDGLEGL-------AAYWEHRH-- 477 LW DMNEPS F G P D Y P G + + A ++ +RH Sbjct: 122 PFDGLWIDMNEPSNFVQGSTSGCPNTKWDNPPYTPHIIGDKLIDKTLCMSARHYGYRHYD 181 Query: 478 VHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTAEWSFLAASV 537 VH+ YG AT + L + G R +++R+ F + ++ W GDN A W + SV Sbjct: 182 VHSLYGYTETVATMSAL-ESIRGK-RSMVISRSTFPNSGQHGGHWLGDNQATWESMYLSV 239 Query: 538 PMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLYP 597 P L++ + G G+D+ GF EL RW Q AF PF R H+ ++P + Sbjct: 240 PGILNMNMFGIPLVGADICGFLGNTNYELCARWTQLGAFYPFSRNHNTKGATPQDPASFG 299 Query: 598 AATTALIRDANRKRYALLDFWYTLFYEHTVDGLPVMRPLFQHYPDEPATYPIDDQYLLGD 657 ++ R RY +L + YTLF++ G V RPLF +P + T ID Q++ G Sbjct: 300 DKFASMARGVLLTRYRMLPYLYTLFFDAYNMGSTVARPLFFEFPKDAKTLAIDRQFMWGS 359 Query: 658 ALLVKPVTTAGASSVEVYLPGDGPWYDV 685 +LLV PV GAS V Y P D WY+V Sbjct: 360 SLLVTPVLQQGASDVTGYFP-DATWYNV 386 >SB_5524| Best HMM Match : Glyco_hydro_31 (HMM E-Value=1.7e-10) Length = 718 Score = 87.4 bits (207), Expect = 4e-17 Identities = 47/147 (31%), Positives = 75/147 (51%), Gaps = 2/147 (1%) Query: 256 GIVDMFVLMGSTPGDAFRQYTALTGTTPLPPKFSLGYHQCRWNYMDEADVRSVDENFDVH 315 GI+D +V +G +P QYT G T +PP +SLG+ RW Y V+ V E+ + Sbjct: 465 GILDFYVFLGPSPESVIEQYTEAIGRTFMPPYWSLGFMLSRWGYNSLKRVQDVVESMRKY 524 Query: 316 DIPMDSIWLDIEYTNKKMYFTWDVVKFPHPAEMVANLTAKGRKMVVIVDPHI--KREPGY 373 DIP D + DI+Y +++ FT++ F E V ++ G + ++I+DP I Y Sbjct: 525 DIPQDVQFGDIDYMVRQLDFTYNKDNFTRLPEWVRSVKKDGLRYIIILDPAIGANETAPY 584 Query: 374 FLHEDATELGYYVKDRDGKDYEGWCWP 400 ++ ++ +VKD K G WP Sbjct: 585 RPYDLGNQMDIWVKDMYNKTLFGKVWP 611 >SB_28911| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 710 Score = 61.3 bits (142), Expect = 3e-09 Identities = 59/207 (28%), Positives = 87/207 (42%), Gaps = 32/207 (15%) Query: 525 DNTAEWSF---LAASVPMCLSLAIAGNSFCGSD-VGG----------FFKYPEAELMTRW 570 D T++W L + +P L+L I G F D +GG F P+ EL RW Sbjct: 510 DRTSKWDEKDGLRSVIPATLTLGILGYPFVLPDMIGGNGAQLNSNSDSFNKPDNELYVRW 569 Query: 571 YQAAAFQPFFRAHSHIETKRREPWLYPAATTALIRDANRKRYALLDFWYTLFYEHTVDGL 630 QAA F P + PWLY + T + + R + + + Sbjct: 570 MQAAVFLPTMQFSF-------PPWLYGSNTINIAKRLLDLRAKISETLILFARQVNQSTA 622 Query: 631 PVMRPLFQHYPDEPATYPIDDQYLLGDALLVKPVT---TAGASSVEVYLPGDGPW----- 682 P++RPL+ P++ +D ++L+GD LV PV + +VYLP G W Sbjct: 623 PIIRPLWWVAPNDDIALTLDSEFLVGDRFLVAPVLRPYSENKGKHKVYLP-QGKWKEEFG 681 Query: 683 -YDVDTYVPHKGPRITQPVTISKIPVY 708 + V T VP G I VT+ +P Y Sbjct: 682 DFKVIT-VPKPGQWIEYTVTLEDLPYY 707 >SB_13631| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 89 Score = 41.5 bits (93), Expect = 0.003 Identities = 23/60 (38%), Positives = 32/60 (53%), Gaps = 1/60 (1%) Query: 787 YPTGAWLERVVVAGIRAAPRSARL-QQDGRQTALQMTLHKGNDVLVLRKPAASMARPWTI 845 + T A +ER+V+ GI +P+S L D LQ T K L ++KPA + A WTI Sbjct: 26 FKTKAEIERIVILGISNSPKSVVLTSSDTVNRQLQFTHDKSAYTLTIKKPAVNAASEWTI 85 >SB_8811| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 569 Score = 34.3 bits (75), Expect = 0.42 Identities = 22/85 (25%), Positives = 43/85 (50%), Gaps = 2/85 (2%) Query: 49 ISKDDGKVILVNTQGHKVIITSEPLKLEFLDQNGEVAVVLNENSQLLVEPLRARREKVED 108 IS +DG+++ + K+ E + +FL + E + EN VE L+ + ++ Sbjct: 207 ISIEDGRLLEIEELREKLSRLEE--EKDFLQKMVETLAIAKENLTKEVEGLKESNQLMQK 264 Query: 109 EDGDGANQLDDEEGTWSETFQSHHD 133 + D +QL+D E +E+ +S+ D Sbjct: 265 DSADAKSQLNDIEEKLAESRESNLD 289 >SB_50480| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 78 Score = 33.5 bits (73), Expect = 0.73 Identities = 18/53 (33%), Positives = 32/53 (60%), Gaps = 3/53 (5%) Query: 706 PVYQRGGTIVPRRERVRRSSALMADDPFTLVVALDANNS--ARGSLYIDDGET 756 P++ RGG+I+ ++ ++A +PF L+VA + A G L++DDGE+ Sbjct: 25 PLHIRGGSIIAT-QKPDITTAASRKNPFELIVAKTGKDGEPANGRLFLDDGES 76 >SB_52973| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 973 Score = 32.3 bits (70), Expect = 1.7 Identities = 13/48 (27%), Positives = 28/48 (58%), Gaps = 2/48 (4%) Query: 194 YGSVPVMYAHGPKRTVGVFWHNSAETWIDVVNYGEGNVVSSLVNLVTG 241 Y + +Y KR WH++AE+ ++ +N E ++++ ++L+TG Sbjct: 360 YAANRTLYIDAQKRPSS--WHHTAESIVETINRAESKILTAKLDLLTG 405 >SB_5939| Best HMM Match : BAH (HMM E-Value=7.9e-18) Length = 1086 Score = 32.3 bits (70), Expect = 1.7 Identities = 25/95 (26%), Positives = 46/95 (48%), Gaps = 6/95 (6%) Query: 59 VNTQGHKVIITSEPLKLEFLDQNGEVAVVLNENSQLLVEPLRARREKVEDEDGDGANQLD 118 + T G+K I S P+K LDQN + +N++ +P R ++ E+GD ++ D Sbjct: 1 MKTSGYKFEIDSSPVKDCMLDQNMNSSAEDKKNNETCNKP---HRRSLKAENGD-MSKGD 56 Query: 119 DEEGTWSETFQSHHDSKPRGNEAVSLDVVFPDADH 153 + E ++ + S D + + V +D PD + Sbjct: 57 NNEN--NQIYTSEDDISYQPGDCVYIDSQRPDVPY 89 >SB_33411| Best HMM Match : 7tm_1 (HMM E-Value=0.03) Length = 1020 Score = 31.9 bits (69), Expect = 2.2 Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 2/50 (4%) Query: 385 YVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSDRYRFENFPGTSKDVH 434 Y+KD +Y C +S YP N V R+ + R +PGTS+ VH Sbjct: 434 YIKDIGIPEYYQPCTSKTSEYPGIINLVHQRHRNT--RTSKYPGTSRLVH 481 >SB_52268| Best HMM Match : SecA_PP_bind (HMM E-Value=0.33) Length = 774 Score = 31.1 bits (67), Expect = 3.9 Identities = 15/43 (34%), Positives = 23/43 (53%) Query: 368 KREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFN 410 +REPG+ ++D GY R +D+ G S S+PDF + Sbjct: 126 QREPGFEFYDDVAPRGYNPFKRGLQDFVGGRNNSSFSHPDFLD 168 >SB_3111| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 486 Score = 31.1 bits (67), Expect = 3.9 Identities = 23/63 (36%), Positives = 32/63 (50%), Gaps = 5/63 (7%) Query: 663 PVTTAGASSVEVYLPGDGPWYDVDTYVPHKGPRITQPVTISKIPVYQRGGTIVPRRERVR 722 P A V YLPG P V +P K + T+ +++ IP + GG +P R+R R Sbjct: 190 PTYQAKNQVVMCYLPGKEPGGHV---LPTK--QRTRQFSVTYIPGKEPGGHELPTRQRTR 244 Query: 723 RSS 725 RSS Sbjct: 245 RSS 247 >SB_41449| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 807 Score = 30.3 bits (65), Expect = 6.8 Identities = 16/61 (26%), Positives = 31/61 (50%) Query: 188 DSRMAIYGSVPVMYAHGPKRTVGVFWHNSAETWIDVVNYGEGNVVSSLVNLVTGGQKKRV 247 D MA++G V +M+A ++ + + +DV+NY N V+ +++ + K V Sbjct: 302 DEFMAVHGDVTLMFAIDTTGSMSAEIETAKKIAVDVINYPRNNPVNYILSPFSDPGAKFV 361 Query: 248 D 248 D Sbjct: 362 D 362 >SB_8156| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 228 Score = 29.9 bits (64), Expect = 9.0 Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 4/58 (6%) Query: 694 PRITQPVTISKIPVYQRGGTIVPRRERVRRSSALMADDPFTLVVALDANNSARGSLYI 751 P++ + I K+ V GG R V SS+ + FTLV + +S RGS Y+ Sbjct: 162 PKLESTMNIIKLEVCYDGGN----RNAVTASSSCLIGARFTLVALICKRSSIRGSWYL 215 >SB_3142| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 2209 Score = 29.9 bits (64), Expect = 9.0 Identities = 30/113 (26%), Positives = 43/113 (38%), Gaps = 12/113 (10%) Query: 363 VDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSDRYR 422 VD PG L E T G + + D + PGSS P P +S++ +Y Sbjct: 81 VDYGASSRPGASL-EPMTVFGLFAQKSDSQSKRRISDPGSSVEPQPCQPSLSQHSLSQYD 139 Query: 423 FEN---------FPGTSKDVHL--WNDMNEPSVFNGPEVTMPKDCRHYKPPQD 464 F GT+ V + N M EP N P PK +++ Q+ Sbjct: 140 MSETLSVAKAFGFEGTNPFVSMETTNSMGEPRASNVPPPVPPKTRKYFDRQQN 192 >SB_20018| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 271 Score = 29.9 bits (64), Expect = 9.0 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%) Query: 75 LEFLDQNGEVAVVLNENSQLLVEPLRARREKVEDEDGDGANQLDDEEGTWSETFQSHHDS 134 LE +D++GEV V E + + + R ++EDE D N DDE + T + Sbjct: 28 LEDIDEDGEVKVKTKEKKKTVTKKKRKGGIQLEDEVDDDDND-DDEVDLDATTAEELAKK 86 Query: 135 KPRGNEA 141 K EA Sbjct: 87 KKEKEEA 93 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.319 0.137 0.428 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 31,290,770 Number of Sequences: 59808 Number of extensions: 1450136 Number of successful extensions: 3121 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 3086 Number of HSP's gapped (non-prelim): 37 length of query: 849 length of database: 16,821,457 effective HSP length: 88 effective length of query: 761 effective length of database: 11,558,353 effective search space: 8795906633 effective search space used: 8795906633 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 64 (29.9 bits)
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