Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000132-TA|BGIBMGA000132-PA|IPR000322|Glycoside
hydrolase, family 31
(849 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 654 0.0
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 192 2e-49
SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 183 8e-47
SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 175 2e-44
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 28 4.7
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 28 4.7
SPAC17G6.03 |||phosphoprotein phosphatase|Schizosaccharomyces po... 28 6.2
SPAC2F7.07c |||histone deacetylase complex subunit Rco1 |Schizos... 27 8.2
SPAC11D3.10 |||nifs homolog|Schizosaccharomyces pombe|chr 1|||Ma... 27 8.2
>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 923
Score = 654 bits (1617), Expect = 0.0
Identities = 317/710 (44%), Positives = 434/710 (61%), Gaps = 44/710 (6%)
Query: 66 VIITSEPLKLEFLDQNGEVAVVLNENSQLLVEPLRARREKVEDEDGDGANQLDDEEGTWS 125
VI+T P K+EF ++GE VVLNE L +E R + + +++ +G W
Sbjct: 168 VIVTFSPFKVEF-QRDGEPQVVLNERHLLNMEYYRPKSSRTPEQEANGM---------WD 217
Query: 126 ETFQSHHDSKPRGNEAVSLDVVFPDADHVYGIPEHTDGLALKTTTSGD-----PYRLYNL 180
ETF + HDSKP+G E+V LD+ F D +VYG+PEHT L+LK T + D PYRLYN+
Sbjct: 218 ETFDNFHDSKPKGPESVGLDIKFVDYGNVYGVPEHTSSLSLKETNNSDAGYTEPYRLYNV 277
Query: 181 DVFEYILDSRMAIYGSVPVMYAHGPKRTVGVFWHNSAETWIDVVNYGEGNVVSSLVNLVT 240
D+FEY +DS M+ YG++P M AH P V VFW N+A TWIDV + S
Sbjct: 278 DLFEYEVDSPMSQYGAIPFMQAHKPNSDVAVFWSNAAATWIDVEKESGPSPHS------- 330
Query: 241 GGQKKRVDARFLSESGIVDMFVLMGSTPGDAFRQYTALTGTTPLPPKFSLGYHQCRWNYM 300
+ + SESG +D+F+ +G D + Y+AL G LPP FS+GYHQCRWNY+
Sbjct: 331 ----QSTSTHWYSESGTLDLFIFLGPKASDVYESYSALVGRPLLPPLFSIGYHQCRWNYV 386
Query: 301 DEADVRSVDENFDVHDIPMDSIWLDIEYTNKKMYFTWDVVKFPHPAEMVANLTAKGRKMV 360
E DV +VD FD D+P D+IWLDIEY +K+ YFTWD FP+P M+ L +K RK++
Sbjct: 387 SEEDVLNVDAKFDEVDMPYDTIWLDIEYASKRRYFTWDKATFPNPKAMLEKLDSKSRKLI 446
Query: 361 VIVDPHIKREPGYFLHEDATELGYYVKDRDGKD-YEGWCWPGSSSYPDFFNPVVSRYYSD 419
VI+DPHIK +P YF+ ++ + Y VKD+ G D Y CWPG+S + DFFNP ++
Sbjct: 447 VILDPHIKNDPNYFVSKELIDYNYAVKDKSGVDNYNADCWPGNSVWVDFFNPEAQAWWGS 506
Query: 420 RYRFENFPGTSKDVHLWNDMNEPSVFNGPEVTMPKDCRHYKPPQDGLEGLAAYWEHRHVH 479
Y F+ F + K++ +WNDMNEPSVF GPE +M +D HY WEHR +H
Sbjct: 507 LYEFDRFE-SDKNLWIWNDMNEPSVFRGPETSMHRDAIHY-----------GGWEHRDIH 554
Query: 480 NEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTAEWSFLAASVPM 539
N YG + T GL+ R +G RPF+LTR+ FAGT +A W GD W L S+P
Sbjct: 555 NIYGHKCINGTYNGLIKRGEGAVRPFILTRSFFAGTSALAANWIGDTMTTWEHLRGSIPT 614
Query: 540 CLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLYPAA 599
L+ I+G +F G+DV GFF P+AEL RWY+ A F PFFRAH+HI+TKRREPWLY
Sbjct: 615 VLTNGISGMAFSGADVAGFFGNPDAELFVRWYETAIFYPFFRAHAHIDTKRREPWLYGEP 674
Query: 600 TTALIRDANRKRYALLDFWYTLFYEHTVDGLPVMRPLFQHYPDEPATYPIDDQYLLGDA- 658
T+L+R+ R RY LL WYT FY G P++ P F +P++ + IDDQ+ +GD+
Sbjct: 675 YTSLVRELLRIRYRLLPTWYTAFYNSHTHGFPILYPQFLMHPEDEEGFAIDDQFYVGDSG 734
Query: 659 LLVKPVTTAGASSVEVYLPGDGPWYDVDTYVPH--KGPRITQPVTISKIPVYQRGGTIVP 716
LLVKPVT + +YL D ++D+ + + KG ++ P + ++PV RGG I+
Sbjct: 735 LLVKPVTHPSIDKITIYLADDEVYFDLHDHTEYAGKGHQVV-PAPLGRVPVLLRGGNILI 793
Query: 717 RRERVRRSSALMADDPFTLVVALD-ANNSARGSLYIDDGETYEYKNNKYI 765
RER+RR++ L +DPFTL +A+ +A G LY+DDG T+ YK +Y+
Sbjct: 794 TRERIRRAAELTRNDPFTLTIAVSKIGKNASGFLYLDDGVTFNYKKGEYL 843
>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 995
Score = 192 bits (467), Expect = 2e-49
Identities = 104/294 (35%), Positives = 159/294 (54%), Gaps = 8/294 (2%)
Query: 477 HVHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTAEWSFLAAS 536
++ N YG R T L V RPF+L+R+ F G+ +Y+A W GDN + WS + S
Sbjct: 622 NLFNTYGYDQSRVTYDSLTSIEPNV-RPFILSRSTFVGSGKYAAHWLGDNYSLWSNMIFS 680
Query: 537 VPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLY 596
+P L+ + G G+DV GF + EL +RW AF PF+R H+ + + +EP+ +
Sbjct: 681 IPGALTFNMVGLPMVGADVCGFMGNTDEELCSRWMALGAFLPFYRNHNSLGSISQEPYRW 740
Query: 597 PAATTALIRDANRKRYALLDFWYTLFYEHTVDGLPVMRPLFQHYPDEPATYPIDDQYLLG 656
+ + R A RY+LL +WYTL YE + GLP++RPLF +P+EP+ D Q+++G
Sbjct: 741 ESVAES-SRCAMNIRYSLLPYWYTLMYEASSQGLPLIRPLFFEFPNEPSLANADRQFMVG 799
Query: 657 DALLVKPVTTAGASSVEVYLPGDG-----PWYDVDTYVPHKGPRITQPVTISKIPVYQRG 711
ALLV PV V PGD WYD IT ++ I V RG
Sbjct: 800 SALLVTPVLEPNVDYVRGVFPGDNSTIWYDWYDHKVIYRQHNENITLSAPLTHINVAIRG 859
Query: 712 GTIVPRRERVRRSSALMADDPFTLVVALDANNSARGSLYIDDGETYEYKNNKYI 765
G I+P ++ ++ +P+ L+VALD++ A GSLY+DDG + + ++ ++
Sbjct: 860 GNIIP-MQKPSLTTHETKQNPYDLLVALDSDRKACGSLYVDDGVSIQQESTLFV 912
Score = 101 bits (241), Expect = 6e-22
Identities = 62/209 (29%), Positives = 102/209 (48%), Gaps = 21/209 (10%)
Query: 256 GIVDMFVLMGST--PGDAFRQYTALTGTTPLPPKFSLGYHQCRWNYMDEADVRSVDENFD 313
GIVD+++ +G T P D Q+ GT + ++ G+H CRW Y + D+ V ENF
Sbjct: 300 GIVDLYIYVGGTKNPKDTVSQFVQSVGTPAMQQHWTFGFHICRWGYKNVFDLVEVKENFK 359
Query: 314 VHDIPMDSIWLDIEYTNKKMYFTWDVVKFPHP--AEMVANLTAKGRKMVVIVD------- 364
+IP+D+ W DI+Y + FT + FP E +L + V I+D
Sbjct: 360 NFEIPVDTFWSDIDYMYEYRDFTVESNAFPKDKMMEFFNSLQQSNQHYVPIIDAAIYAAN 419
Query: 365 PHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSD----- 419
P + + Y+ + + ++++ D Y G WPG +++PDF NP + Y+++
Sbjct: 420 PINRSDDVYYPYYEGVRRDIFLRNPDRSLYVGNVWPGFTTFPDFTNPETTNYWTECLMNL 479
Query: 420 --RYRF-ENFPGTSKDVHLWNDMNEPSVF 445
+ + +FP LW DMNEP+ F
Sbjct: 480 SAAFGYNSSFPLPYSG--LWIDMNEPTSF 506
>SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 993
Score = 183 bits (446), Expect = 8e-47
Identities = 109/353 (30%), Positives = 184/353 (52%), Gaps = 16/353 (4%)
Query: 503 RPFLLTRAVFAGTQRYSAVWTGDNTAEWSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYP 562
RPFLL+R+ F G+ RY+A W GDN ++WS + +S+ L+ + G G+DV G+
Sbjct: 650 RPFLLSRSTFVGSGRYAAHWLGDNKSQWSDMVSSISSILTFNLLGIPMVGADVCGYNGNT 709
Query: 563 EAELMTRWYQAAAFQPFFRAHSHIETKRREPWLYPAATTALIRDANRKRYALLDFWYTLF 622
+ EL RW AF PF+R H+ + + +EP+ + A+ R A RY+LL +WYTL
Sbjct: 710 DEELCARWMALGAFLPFYRNHNSLGSIPQEPFRW-ASVAEASRSAIEIRYSLLPYWYTLM 768
Query: 623 YEHTVDGLPVMRPLFQHYPDEPATYPIDDQYLLGDALLVKPVTTAGASSVEVYLPGDGP- 681
+ +VDG P++RPLF +P + + +D Q+++G ALL+ P + ++ +PGD
Sbjct: 769 HTASVDGTPMVRPLFFEFPKQISLASVDKQFMIGTALLISPALEPNTTYIQGIIPGDNDT 828
Query: 682 -WYD--VDTYVPHK-GPRITQPVTISKIPVYQRGGTIVPRRERVRRSSALMADDPFTLVV 737
WYD + + H IT + + + RGG I+P ++ ++ ++P++L++
Sbjct: 829 IWYDWYNHSVINHDYDENITMSAPLGYVNIAVRGGNIIP-LQQPGYTTYESRNNPYSLLI 887
Query: 738 ALDANNSARGSLYIDDGETYEYKNNKYIYAKIEFGPDEMTYTLASEGAQYPTGAWLERVV 797
A+D N A GSLYIDDG + N + K+ + +T ++ P+ L +
Sbjct: 888 AMDNNGFASGSLYIDDG--ISMQTNSSLSVKLNSNSNTITCVVSGTMVSSPS---LANIT 942
Query: 798 VAGIRAAPRSARL--QQDGRQTALQMTLHKGNDVLVLRKPAASMARPWTITFA 848
+ G+ P + QQ TL N +L L A ++ WT+T++
Sbjct: 943 ILGLSNPPNTILFNGQQLSDYQYSDQTLSLTN-LLDLTVDGA-FSKNWTVTWS 993
Score = 95.1 bits (226), Expect = 4e-20
Identities = 53/173 (30%), Positives = 84/173 (48%), Gaps = 11/173 (6%)
Query: 256 GIVDMFVLMGST--PGDAFRQYTALTGTTPLPPKFSLGYHQCRWNYMDEADVRSVDENFD 313
GI+D+FV GST P +QY GT + P +SLG+ RW Y +D+ ++ +
Sbjct: 302 GIIDLFVYSGSTVSPKYTIQQYVQSIGTPTMQPYWSLGFQMSRWGYKTLSDLINMRSYLN 361
Query: 314 VHDIPMDSIWLDIEYTNKKMYFTWDVVKFP--HPAEMVANLTAKGRKMVVIVDPHI---- 367
+IP + W DI+Y ++ FT + FP + +L + V ++DP I
Sbjct: 362 ASNIPTEGFWNDIDYMSEFRTFTVNSTAFPPNQTLDFFRSLDESHQHYVPVLDPAIYAAN 421
Query: 368 ---KREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYY 417
+ Y+ + E ++K+ +G Y G WPG YPDF NP V +Y+
Sbjct: 422 PNKSADRTYYPYYSGFEDNIFIKNPNGSAYVGMAWPGFVVYPDFTNPAVLQYW 474
>SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 175 bits (426), Expect = 2e-44
Identities = 106/338 (31%), Positives = 165/338 (48%), Gaps = 13/338 (3%)
Query: 478 VHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTAEWSFLAASV 537
+ N YG + + L + RPF+L+R+ F G+ Y A W GDN + WS + S+
Sbjct: 601 IFNMYGYGETKVSYAALTQISPNE-RPFILSRSTFLGSGVYGAHWLGDNHSLWSNMFFSI 659
Query: 538 PMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLYP 597
+ + G G+DV GF + EL +RW AF PF+R H++I +EP+ +
Sbjct: 660 SGMIVFNMMGIPMVGADVCGFLGDSDEELCSRWMAMGAFSPFYRNHNNIYQISQEPYTWS 719
Query: 598 AATTALIRDANRKRYALLDFWYTLFYEHTVDGLPVMRPLFQHYPDEPATYPIDDQYLLGD 657
+ A R A RY+LL +WYT+ + + DG P +R LF +P++P +D Q+++GD
Sbjct: 720 SVAEA-SRRAMYIRYSLLPYWYTIMAKASQDGTPALRALFVEFPNDPTLADVDRQFMVGD 778
Query: 658 ALLVKPVTTAGASSVEVYLPGDG-----PWYDVDTYVPHKGPRITQPVTISKIPVYQRGG 712
+LLV PV V+ PGD WY+ V +T + I V RGG
Sbjct: 779 SLLVTPVLEPNVEYVQGVFPGDNSTVWYDWYNHTEIVRQYNENVTLYAPLEHINVAIRGG 838
Query: 713 TIVPRRERVRRSSALMADDPFTLVVALDANNSARGSLYIDDGETYEYKNNKYIYAKIEFG 772
+++P ++ ++ +PF L+VALD + SA G LY+DDG + E N + F
Sbjct: 839 SVLP-MQQPSLTTYESRQNPFNLLVALDRDGSATGELYLDDGVSIEL--NATLSVSFTFS 895
Query: 773 PDEMTYTLASEGAQYPTGAWLERVVVAGIRAAPRSARL 810
++ A Y L V + G+ +P S L
Sbjct: 896 DGVLS---AVPTGSYEVSQPLANVTILGLTESPSSITL 930
Score = 115 bits (277), Expect = 2e-26
Identities = 66/206 (32%), Positives = 104/206 (50%), Gaps = 17/206 (8%)
Query: 256 GIVDMFVLMGST--PGDAFRQYTALTGTTPLPPKFSLGYHQCRWNYMDEADVRSVDENFD 313
G++D+FV GST P + +Q+ G + ++LGYH CRW Y + ++ V +N+
Sbjct: 284 GVIDLFVYSGSTESPKETVKQFVQSIGKPAMHQYWTLGYHSCRWGYTNITEIMDVRQNYI 343
Query: 314 VHDIPMDSIWLDIEYTNKKMYFTWDVVKFPHP--AEMVANLTAKGRKMVVIVD------- 364
DIP+++ W DI+Y K FT D V + ++L + + V I+D
Sbjct: 344 DADIPVETFWSDIDYMEKYRDFTVDPVSYSKSDMQTFFSDLVSNHQHYVPIIDAAIYAAN 403
Query: 365 PHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSD----- 419
P+ + Y+ + E ++K+ +G Y G WPG +++PDF NP V Y+ D
Sbjct: 404 PYNHTDDSYYPYYAGVEKDIFLKNPNGSIYIGAVWPGFTAFPDFTNPDVVDYWKDCLINL 463
Query: 420 RYRFENFPGTSKDVHLWNDMNEPSVF 445
Y F + GT +W DMNEPS F
Sbjct: 464 TYAFGS-NGTVPFSGIWTDMNEPSSF 488
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Query: 46 LKLISKDD-GKVILVNTQGHKVIITSEPLKLEFLDQNGEVAVVLNENSQLLV 96
L ++ K + GKV+L + K + + LK EF+ +N EV +E LV
Sbjct: 686 LSVLGKGNFGKVMLAELKSEKQLYAIKVLKKEFILENDEVESTKSEKRVFLV 737
>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/27 (48%), Positives = 16/27 (59%)
Query: 3 WKYAFKLSALADGTFRVELDEADPLYP 29
W YA KL L D + R E++E DP P
Sbjct: 939 WDYAKKLDFLIDVSDRFEVEERDPPSP 965
>SPAC17G6.03 |||phosphoprotein phosphatase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 635
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/46 (30%), Positives = 23/46 (50%), Gaps = 2/46 (4%)
Query: 110 DGDGANQLDDEEGTWSETFQSH--HDSKPRGNEAVSLDVVFPDADH 153
DG G + L D G+W+++ ++ +D GN + L V D H
Sbjct: 94 DGTGLSDLTDPPGSWTDSLLANMDYDILVAGNHELYLSTVANDTYH 139
>SPAC2F7.07c |||histone deacetylase complex subunit Rco1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 607
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/52 (26%), Positives = 29/52 (55%), Gaps = 4/52 (7%)
Query: 50 SKDDGKVILVNTQGHKVIITSEPLKLEFLDQNGEVAV----VLNENSQLLVE 97
++D+ V + QG ++ S+P KL FL+Q + + ++ ++ QL +E
Sbjct: 492 NEDEPSVQTIQLQGKIRVVPSKPFKLNFLEQIRDNVINLRKMVEQDEQLCIE 543
>SPAC11D3.10 |||nifs homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 434
Score = 27.5 bits (58), Expect = 8.2
Identities = 25/82 (30%), Positives = 33/82 (40%), Gaps = 3/82 (3%)
Query: 128 FQSHHDSKPRGNEAVSLDVVFPDADHVYGIPEHTDGLALKTTTSGDPYRLYNLDVFEYIL 187
FQ KP G+ V LD P+ + I DGL ++ + Y N F +
Sbjct: 95 FQRSLKWKP-GDNVVILDNEHPNQGFGW-IALQNDGLEVRLVPNEGQYHA-NASTFAPYV 151
Query: 188 DSRMAIYGSVPVMYAHGPKRTV 209
DSR G VM+ G K V
Sbjct: 152 DSRTKAIGLSSVMFHSGQKNDV 173
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.319 0.137 0.428
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,256,566
Number of Sequences: 5004
Number of extensions: 199679
Number of successful extensions: 434
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 397
Number of HSP's gapped (non-prelim): 15
length of query: 849
length of database: 2,362,478
effective HSP length: 79
effective length of query: 770
effective length of database: 1,967,162
effective search space: 1514714740
effective search space used: 1514714740
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 58 (27.5 bits)
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