SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000132-TA|BGIBMGA000132-PA|IPR000322|Glycoside
hydrolase, family 31
         (849 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_13633| Best HMM Match : Glyco_hydro_31 (HMM E-Value=0)             348   1e-95
SB_27817| Best HMM Match : Glyco_hydro_31 (HMM E-Value=0)             185   2e-46
SB_5524| Best HMM Match : Glyco_hydro_31 (HMM E-Value=1.7e-10)         87   4e-17
SB_28911| Best HMM Match : No HMM Matches (HMM E-Value=.)              61   3e-09
SB_13631| Best HMM Match : No HMM Matches (HMM E-Value=.)              42   0.003
SB_8811| Best HMM Match : No HMM Matches (HMM E-Value=.)               34   0.42 
SB_50480| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   0.73 
SB_52973| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   1.7  
SB_5939| Best HMM Match : BAH (HMM E-Value=7.9e-18)                    32   1.7  
SB_33411| Best HMM Match : 7tm_1 (HMM E-Value=0.03)                    32   2.2  
SB_52268| Best HMM Match : SecA_PP_bind (HMM E-Value=0.33)             31   3.9  
SB_3111| Best HMM Match : No HMM Matches (HMM E-Value=.)               31   3.9  
SB_41449| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   6.8  
SB_8156| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   9.0  
SB_3142| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   9.0  
SB_20018| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   9.0  

>SB_13633| Best HMM Match : Glyco_hydro_31 (HMM E-Value=0)
          Length = 663

 Score =  348 bits (855), Expect = 1e-95
 Identities = 185/406 (45%), Positives = 249/406 (61%), Gaps = 41/406 (10%)

Query: 2   NWKYAFKLSALADGTFRVELDEADPLYPRYRTQLALDGEPKADGLK-----------LIS 50
           N +    LS L   T R +++E  P+ PRY  + AL  EPK   +             I+
Sbjct: 70  NVELHLSLSTLEKNTARFKINELKPIRPRYEVKDALKTEPKLVRMNHKSINMFVSSYKIT 129

Query: 51  KDDGKVILVN-TQGHKVIITSEPLKLEFLDQNGEVAVVLNENSQLLVEPLRARR--EKVE 107
           K++   + V+  +   +I+T +P +L+FL  N EV V +N    L  E  RA++  E  +
Sbjct: 130 KEEANSMHVSFDENSNIIVTYKPFRLDFLVGN-EVVVSINTRGLLNFEHYRAKKVGEPPK 188

Query: 108 DE------------DGDGANQ---------LDDE---EGTWSETFQSHHDSKPRGNEAVS 143
           +E            +GD  N+         ++DE   EG W E F+ HHDSKPRG  +V 
Sbjct: 189 EEPLQPAPPGPPSIEGDEPNEEQKQEEEKVVEDEKHEEGMWEENFKEHHDSKPRGPSSVG 248

Query: 144 LDVVFPDADHVYGIPEHTDGLALKTTTSGDPYRLYNLDVFEYILDSRMAIYGSVPVMYAH 203
           LD+ FP  +HVYGIPEH D LALK+T   +PYRLYNLDVFEY LDSRMA+YGS+PVM AH
Sbjct: 249 LDISFPGVEHVYGIPEHADSLALKSTIGSEPYRLYNLDVFEYELDSRMALYGSIPVMLAH 308

Query: 204 GPKRTVGVFWHNSAETWIDVVNY-GEGNVVSSLVNLVTGGQK-KRVDARFLSESGIVDMF 261
              +TVGVFW N+AETW+D+       ++++SL++     ++  +VD  + SESGI+D+F
Sbjct: 309 SASKTVGVFWMNAAETWVDISTADARKSMLNSLMSFFKAEEEVPQVDTHWFSESGIIDVF 368

Query: 262 VLMGSTPGDAFRQYTALTGTTPLPPKFSLGYHQCRWNYMDEADVRSVDENFDVHDIPMDS 321
           V+MG  P D F+QY +LTG T LPP F + YHQCRWNY DE DV+SVD++FD HDIP D 
Sbjct: 369 VMMGPRPHDVFKQYASLTGPTSLPPLFGIAYHQCRWNYNDEEDVKSVDDSFDKHDIPYDV 428

Query: 322 IWLDIEYTNKKMYFTWDVVKFPHPAEMVANLTAKGRKMVVIVDPHI 367
           +WLDIE+T+ K Y TWD VKFP+P  M  N+ +KGRK  V   P I
Sbjct: 429 LWLDIEHTDGKRYMTWDKVKFPNPEAMQENIASKGRKPSVFHGPEI 474



 Score =  243 bits (595), Expect = 4e-64
 Identities = 117/221 (52%), Positives = 149/221 (67%), Gaps = 14/221 (6%)

Query: 415 RYYS-DRYRFENFPGTSKDVHLWNDMNEPSVFNGPEVTMPKDCRHYKPPQDGLEGLAAYW 473
           RY + D+ +F N P   ++ ++ +   +PSVF+GPE+TM KD  HY             W
Sbjct: 440 RYMTWDKVKFPN-PEAMQE-NIASKGRKPSVFHGPEITMHKDTIHYGD-----------W 486

Query: 474 EHRHVHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTAEWSFL 533
           EHR VHN YG++  +ATN GL+ R+ G  RPF+L+RA FAGTQRY  +WTGDN AEWS L
Sbjct: 487 EHRDVHNIYGMYFHKATNLGLIQRSGGKDRPFVLSRAFFAGTQRYGPIWTGDNMAEWSHL 546

Query: 534 AASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREP 593
            AS+PM LSL + G  F G+DVGGFFK PE EL+ RWYQ   F PF RAH+H++TKRREP
Sbjct: 547 KASIPMILSLGVTGLPFAGADVGGFFKNPEPELLARWYQTGVFTPFLRAHAHLDTKRREP 606

Query: 594 WLYPAATTALIRDANRKRYALLDFWYTLFYEHTVDGLPVMR 634
           WL+      +IRDA R RYALL  WYTLF+  + DG P++R
Sbjct: 607 WLFDDVYKNVIRDALRTRYALLPLWYTLFFHASQDGTPIIR 647


>SB_27817| Best HMM Match : Glyco_hydro_31 (HMM E-Value=0)
          Length = 388

 Score =  185 bits (450), Expect = 2e-46
 Identities = 129/388 (33%), Positives = 182/388 (46%), Gaps = 22/388 (5%)

Query: 315 HDIPMDSIWLDIEYTNKKMYFTWDVVKFPHPAEMVANL-TAKGRKMVVIVDPHIKR-EPG 372
           + IP D  W DIEY    + FT D   +    + V  L T   +  + IVDP I   +P 
Sbjct: 4   YKIPQDVQWNDIEYMKDHLDFTVDSTNWGGLGDFVKKLHTQYDQHYIPIVDPGISNTQPS 63

Query: 373 --YFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSDRYRFENFPGTS 430
             Y  + D   +G +V   +G    G  WPG++ YPDFFNP    Y++ +     F    
Sbjct: 64  GSYPPYSDGLAMGVFVNASNGGPIVGQVWPGNTVYPDFFNPSTQSYWTKQ--ISQFHDVV 121

Query: 431 KDVHLWNDMNEPSVF-NGPEVTMPK---DCRHYKPPQDGLEGL-------AAYWEHRH-- 477
               LW DMNEPS F  G     P    D   Y P   G + +       A ++ +RH  
Sbjct: 122 PFDGLWIDMNEPSNFVQGSTSGCPNTKWDNPPYTPHIIGDKLIDKTLCMSARHYGYRHYD 181

Query: 478 VHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTAEWSFLAASV 537
           VH+ YG     AT + L +   G  R  +++R+ F  + ++   W GDN A W  +  SV
Sbjct: 182 VHSLYGYTETVATMSAL-ESIRGK-RSMVISRSTFPNSGQHGGHWLGDNQATWESMYLSV 239

Query: 538 PMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLYP 597
           P  L++ + G    G+D+ GF      EL  RW Q  AF PF R H+      ++P  + 
Sbjct: 240 PGILNMNMFGIPLVGADICGFLGNTNYELCARWTQLGAFYPFSRNHNTKGATPQDPASFG 299

Query: 598 AATTALIRDANRKRYALLDFWYTLFYEHTVDGLPVMRPLFQHYPDEPATYPIDDQYLLGD 657
               ++ R     RY +L + YTLF++    G  V RPLF  +P +  T  ID Q++ G 
Sbjct: 300 DKFASMARGVLLTRYRMLPYLYTLFFDAYNMGSTVARPLFFEFPKDAKTLAIDRQFMWGS 359

Query: 658 ALLVKPVTTAGASSVEVYLPGDGPWYDV 685
           +LLV PV   GAS V  Y P D  WY+V
Sbjct: 360 SLLVTPVLQQGASDVTGYFP-DATWYNV 386


>SB_5524| Best HMM Match : Glyco_hydro_31 (HMM E-Value=1.7e-10)
          Length = 718

 Score = 87.4 bits (207), Expect = 4e-17
 Identities = 47/147 (31%), Positives = 75/147 (51%), Gaps = 2/147 (1%)

Query: 256 GIVDMFVLMGSTPGDAFRQYTALTGTTPLPPKFSLGYHQCRWNYMDEADVRSVDENFDVH 315
           GI+D +V +G +P     QYT   G T +PP +SLG+   RW Y     V+ V E+   +
Sbjct: 465 GILDFYVFLGPSPESVIEQYTEAIGRTFMPPYWSLGFMLSRWGYNSLKRVQDVVESMRKY 524

Query: 316 DIPMDSIWLDIEYTNKKMYFTWDVVKFPHPAEMVANLTAKGRKMVVIVDPHI--KREPGY 373
           DIP D  + DI+Y  +++ FT++   F    E V ++   G + ++I+DP I       Y
Sbjct: 525 DIPQDVQFGDIDYMVRQLDFTYNKDNFTRLPEWVRSVKKDGLRYIIILDPAIGANETAPY 584

Query: 374 FLHEDATELGYYVKDRDGKDYEGWCWP 400
             ++   ++  +VKD   K   G  WP
Sbjct: 585 RPYDLGNQMDIWVKDMYNKTLFGKVWP 611


>SB_28911| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 710

 Score = 61.3 bits (142), Expect = 3e-09
 Identities = 59/207 (28%), Positives = 87/207 (42%), Gaps = 32/207 (15%)

Query: 525 DNTAEWSF---LAASVPMCLSLAIAGNSFCGSD-VGG----------FFKYPEAELMTRW 570
           D T++W     L + +P  L+L I G  F   D +GG           F  P+ EL  RW
Sbjct: 510 DRTSKWDEKDGLRSVIPATLTLGILGYPFVLPDMIGGNGAQLNSNSDSFNKPDNELYVRW 569

Query: 571 YQAAAFQPFFRAHSHIETKRREPWLYPAATTALIRDANRKRYALLDFWYTLFYEHTVDGL 630
            QAA F P  +           PWLY + T  + +     R  + +       +      
Sbjct: 570 MQAAVFLPTMQFSF-------PPWLYGSNTINIAKRLLDLRAKISETLILFARQVNQSTA 622

Query: 631 PVMRPLFQHYPDEPATYPIDDQYLLGDALLVKPVT---TAGASSVEVYLPGDGPW----- 682
           P++RPL+   P++     +D ++L+GD  LV PV    +      +VYLP  G W     
Sbjct: 623 PIIRPLWWVAPNDDIALTLDSEFLVGDRFLVAPVLRPYSENKGKHKVYLP-QGKWKEEFG 681

Query: 683 -YDVDTYVPHKGPRITQPVTISKIPVY 708
            + V T VP  G  I   VT+  +P Y
Sbjct: 682 DFKVIT-VPKPGQWIEYTVTLEDLPYY 707


>SB_13631| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 89

 Score = 41.5 bits (93), Expect = 0.003
 Identities = 23/60 (38%), Positives = 32/60 (53%), Gaps = 1/60 (1%)

Query: 787 YPTGAWLERVVVAGIRAAPRSARL-QQDGRQTALQMTLHKGNDVLVLRKPAASMARPWTI 845
           + T A +ER+V+ GI  +P+S  L   D     LQ T  K    L ++KPA + A  WTI
Sbjct: 26  FKTKAEIERIVILGISNSPKSVVLTSSDTVNRQLQFTHDKSAYTLTIKKPAVNAASEWTI 85


>SB_8811| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 569

 Score = 34.3 bits (75), Expect = 0.42
 Identities = 22/85 (25%), Positives = 43/85 (50%), Gaps = 2/85 (2%)

Query: 49  ISKDDGKVILVNTQGHKVIITSEPLKLEFLDQNGEVAVVLNENSQLLVEPLRARREKVED 108
           IS +DG+++ +     K+    E  + +FL +  E   +  EN    VE L+   + ++ 
Sbjct: 207 ISIEDGRLLEIEELREKLSRLEE--EKDFLQKMVETLAIAKENLTKEVEGLKESNQLMQK 264

Query: 109 EDGDGANQLDDEEGTWSETFQSHHD 133
           +  D  +QL+D E   +E+ +S+ D
Sbjct: 265 DSADAKSQLNDIEEKLAESRESNLD 289


>SB_50480| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 78

 Score = 33.5 bits (73), Expect = 0.73
 Identities = 18/53 (33%), Positives = 32/53 (60%), Gaps = 3/53 (5%)

Query: 706 PVYQRGGTIVPRRERVRRSSALMADDPFTLVVALDANNS--ARGSLYIDDGET 756
           P++ RGG+I+   ++   ++A    +PF L+VA    +   A G L++DDGE+
Sbjct: 25  PLHIRGGSIIAT-QKPDITTAASRKNPFELIVAKTGKDGEPANGRLFLDDGES 76


>SB_52973| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 973

 Score = 32.3 bits (70), Expect = 1.7
 Identities = 13/48 (27%), Positives = 28/48 (58%), Gaps = 2/48 (4%)

Query: 194 YGSVPVMYAHGPKRTVGVFWHNSAETWIDVVNYGEGNVVSSLVNLVTG 241
           Y +   +Y    KR     WH++AE+ ++ +N  E  ++++ ++L+TG
Sbjct: 360 YAANRTLYIDAQKRPSS--WHHTAESIVETINRAESKILTAKLDLLTG 405


>SB_5939| Best HMM Match : BAH (HMM E-Value=7.9e-18)
          Length = 1086

 Score = 32.3 bits (70), Expect = 1.7
 Identities = 25/95 (26%), Positives = 46/95 (48%), Gaps = 6/95 (6%)

Query: 59  VNTQGHKVIITSEPLKLEFLDQNGEVAVVLNENSQLLVEPLRARREKVEDEDGDGANQLD 118
           + T G+K  I S P+K   LDQN   +    +N++   +P    R  ++ E+GD  ++ D
Sbjct: 1   MKTSGYKFEIDSSPVKDCMLDQNMNSSAEDKKNNETCNKP---HRRSLKAENGD-MSKGD 56

Query: 119 DEEGTWSETFQSHHDSKPRGNEAVSLDVVFPDADH 153
           + E   ++ + S  D   +  + V +D   PD  +
Sbjct: 57  NNEN--NQIYTSEDDISYQPGDCVYIDSQRPDVPY 89


>SB_33411| Best HMM Match : 7tm_1 (HMM E-Value=0.03)
          Length = 1020

 Score = 31.9 bits (69), Expect = 2.2
 Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 2/50 (4%)

Query: 385 YVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSDRYRFENFPGTSKDVH 434
           Y+KD    +Y   C   +S YP   N V  R+ +   R   +PGTS+ VH
Sbjct: 434 YIKDIGIPEYYQPCTSKTSEYPGIINLVHQRHRNT--RTSKYPGTSRLVH 481


>SB_52268| Best HMM Match : SecA_PP_bind (HMM E-Value=0.33)
          Length = 774

 Score = 31.1 bits (67), Expect = 3.9
 Identities = 15/43 (34%), Positives = 23/43 (53%)

Query: 368 KREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFN 410
           +REPG+  ++D    GY    R  +D+ G     S S+PDF +
Sbjct: 126 QREPGFEFYDDVAPRGYNPFKRGLQDFVGGRNNSSFSHPDFLD 168


>SB_3111| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 486

 Score = 31.1 bits (67), Expect = 3.9
 Identities = 23/63 (36%), Positives = 32/63 (50%), Gaps = 5/63 (7%)

Query: 663 PVTTAGASSVEVYLPGDGPWYDVDTYVPHKGPRITQPVTISKIPVYQRGGTIVPRRERVR 722
           P   A    V  YLPG  P   V   +P K  + T+  +++ IP  + GG  +P R+R R
Sbjct: 190 PTYQAKNQVVMCYLPGKEPGGHV---LPTK--QRTRQFSVTYIPGKEPGGHELPTRQRTR 244

Query: 723 RSS 725
           RSS
Sbjct: 245 RSS 247


>SB_41449| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 807

 Score = 30.3 bits (65), Expect = 6.8
 Identities = 16/61 (26%), Positives = 31/61 (50%)

Query: 188 DSRMAIYGSVPVMYAHGPKRTVGVFWHNSAETWIDVVNYGEGNVVSSLVNLVTGGQKKRV 247
           D  MA++G V +M+A     ++      + +  +DV+NY   N V+ +++  +    K V
Sbjct: 302 DEFMAVHGDVTLMFAIDTTGSMSAEIETAKKIAVDVINYPRNNPVNYILSPFSDPGAKFV 361

Query: 248 D 248
           D
Sbjct: 362 D 362


>SB_8156| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 228

 Score = 29.9 bits (64), Expect = 9.0
 Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 4/58 (6%)

Query: 694 PRITQPVTISKIPVYQRGGTIVPRRERVRRSSALMADDPFTLVVALDANNSARGSLYI 751
           P++   + I K+ V   GG     R  V  SS+ +    FTLV  +   +S RGS Y+
Sbjct: 162 PKLESTMNIIKLEVCYDGGN----RNAVTASSSCLIGARFTLVALICKRSSIRGSWYL 215


>SB_3142| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2209

 Score = 29.9 bits (64), Expect = 9.0
 Identities = 30/113 (26%), Positives = 43/113 (38%), Gaps = 12/113 (10%)

Query: 363 VDPHIKREPGYFLHEDATELGYYVKDRDGKDYEGWCWPGSSSYPDFFNPVVSRYYSDRYR 422
           VD      PG  L E  T  G + +  D +       PGSS  P    P +S++   +Y 
Sbjct: 81  VDYGASSRPGASL-EPMTVFGLFAQKSDSQSKRRISDPGSSVEPQPCQPSLSQHSLSQYD 139

Query: 423 FEN---------FPGTSKDVHL--WNDMNEPSVFNGPEVTMPKDCRHYKPPQD 464
                       F GT+  V +   N M EP   N P    PK  +++   Q+
Sbjct: 140 MSETLSVAKAFGFEGTNPFVSMETTNSMGEPRASNVPPPVPPKTRKYFDRQQN 192


>SB_20018| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 271

 Score = 29.9 bits (64), Expect = 9.0
 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)

Query: 75  LEFLDQNGEVAVVLNENSQLLVEPLRARREKVEDEDGDGANQLDDEEGTWSETFQSHHDS 134
           LE +D++GEV V   E  + + +  R    ++EDE  D  N  DDE    + T +     
Sbjct: 28  LEDIDEDGEVKVKTKEKKKTVTKKKRKGGIQLEDEVDDDDND-DDEVDLDATTAEELAKK 86

Query: 135 KPRGNEA 141
           K    EA
Sbjct: 87  KKEKEEA 93


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.319    0.137    0.428 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 31,290,770
Number of Sequences: 59808
Number of extensions: 1450136
Number of successful extensions: 3121
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 3086
Number of HSP's gapped (non-prelim): 37
length of query: 849
length of database: 16,821,457
effective HSP length: 88
effective length of query: 761
effective length of database: 11,558,353
effective search space: 8795906633
effective search space used: 8795906633
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 64 (29.9 bits)

- SilkBase 1999-2023 -