BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000129-TA|BGIBMGA000129-PA|IPR002108|Actin-binding, cofilin/tropomyosin type (113 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g59890.1 68418.m07510 actin-depolymerizing factor 4 (ADF4) id... 93 4e-20 At3g46010.1 68416.m04978 actin-depolymerizing factor 1 (ADF1) id... 91 2e-19 At5g59890.2 68418.m07511 actin-depolymerizing factor 4 (ADF4) id... 87 2e-18 At5g59880.1 68418.m07508 actin-depolymerizing factor 3 (ADF3) id... 84 2e-17 At1g01750.1 68414.m00094 actin-depolymerizing factor, putative s... 84 2e-17 At2g31200.1 68415.m03810 actin-depolymerizing factor 6 (ADF6) id... 83 3e-17 At4g00680.1 68417.m00093 actin-depolymerizing factor, putative s... 83 4e-17 At3g46000.1 68416.m04977 actin-depolymerizing factor, putative (... 83 4e-17 At5g52360.1 68418.m06497 actin-depolymerizing factor, putative s... 82 1e-16 At4g25590.1 68417.m03687 actin-depolymerizing factor, putative s... 77 4e-15 At5g59880.2 68418.m07509 actin-depolymerizing factor 3 (ADF3) id... 74 3e-14 At2g16700.1 68415.m01916 actin-depolymerizing factor 5 (ADF5) id... 73 5e-14 At4g34970.1 68417.m04957 actin-depolymerizing factor, putative s... 70 3e-13 At3g45990.1 68416.m04976 actin-depolymerizing factor, putative s... 63 4e-11 At3g03290.1 68416.m00326 universal stress protein (USP) family p... 31 0.14 At1g21630.1 68414.m02708 calcium-binding EF hand family protein ... 29 0.96 At1g71270.1 68414.m08225 Vps52/Sac2 family protein similar to SP... 27 3.9 At1g44835.1 68414.m05136 YbaK/prolyl-tRNA synthetase family prot... 27 3.9 At2g18630.1 68415.m02169 expressed protein unusual splice site a... 26 6.8 At5g61560.1 68418.m07725 protein kinase family protein contains ... 25 9.0 At2g45650.1 68415.m05676 MADS-box protein (AGL6) 25 9.0 >At5g59890.1 68418.m07510 actin-depolymerizing factor 4 (ADF4) identical to SP|Q9ZSK3 Actin-depolymerizing factor 4 (ADF-4) (AtADF4) {Arabidopsis thaliana} Length = 139 Score = 93.1 bits (221), Expect = 4e-20 Identities = 45/108 (41%), Positives = 67/108 (62%), Gaps = 9/108 (8%) Query: 6 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGG 64 ASG+ V D CK + E+K + HR++V+ I + +KQ+ VE VGE YE F L Sbjct: 5 ASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLP--- 61 Query: 65 TGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 ECRY ++DF++ T+E +K K+F ++WCPD AKV+ KM+Y+ Sbjct: 62 ADECRYAIYDFDFV-----TAENCQKSKIFFIAWCPDVAKVRSKMIYA 104 >At3g46010.1 68416.m04978 actin-depolymerizing factor 1 (ADF1) identical to SP|Q39250 Actin-depolymerizing factor 1 (ADF-1) (AtADF1) {Arabidopsis thaliana} Length = 139 Score = 91.1 bits (216), Expect = 2e-19 Identities = 44/108 (40%), Positives = 68/108 (62%), Gaps = 9/108 (8%) Query: 6 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGG 64 ASG+ V D CK + E+K + HR++V+ I + +KQ+ VE VG+ YE+F L Sbjct: 5 ASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLP--- 61 Query: 65 TGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 ECRY ++DF++ T+E +K K+F ++WCPD AKV+ KM+Y+ Sbjct: 62 ADECRYAIYDFDFV-----TAENCQKSKIFFIAWCPDIAKVRSKMIYA 104 >At5g59890.2 68418.m07511 actin-depolymerizing factor 4 (ADF4) identical to SP|Q9ZSK3 Actin-depolymerizing factor 4 (ADF-4) (AtADF4) {Arabidopsis thaliana} Length = 132 Score = 87.4 bits (207), Expect = 2e-18 Identities = 42/105 (40%), Positives = 64/105 (60%), Gaps = 9/105 (8%) Query: 9 VTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGGTGE 67 + V D CK + E+K + HR++V+ I + +KQ+ VE VGE YE F L E Sbjct: 1 MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLP---ADE 57 Query: 68 CRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 CRY ++DF++ T+E +K K+F ++WCPD AKV+ KM+Y+ Sbjct: 58 CRYAIYDFDFV-----TAENCQKSKIFFIAWCPDVAKVRSKMIYA 97 >At5g59880.1 68418.m07508 actin-depolymerizing factor 3 (ADF3) identical to SP|Q9ZSK4 Actin-depolymerizing factor 3 (ADF 3) (AtADF3) {Arabidopsis thaliana} Length = 139 Score = 84.2 bits (199), Expect = 2e-17 Identities = 39/108 (36%), Positives = 65/108 (60%), Gaps = 9/108 (8%) Query: 6 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGG 64 ASG+ V D CK + E+K + HR++++ I + +KQ+ VE +GE +E L Sbjct: 5 ASGMAVHDDCKLKFMELKTKRTHRFIIYKIEELQKQVIVEKIGEPGQTHEDLAASLP--- 61 Query: 65 TGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 ECRY +FDF++ +SE + ++F ++W PDTA+V+ KM+Y+ Sbjct: 62 ADECRYAIFDFDFV-----SSEGVPRSRIFFVAWSPDTARVRSKMIYA 104 >At1g01750.1 68414.m00094 actin-depolymerizing factor, putative strong similarity to SP|P30175 Actin-depolymerizing factor (ADF) {Lilium longiflorum}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 140 Score = 83.8 bits (198), Expect = 2e-17 Identities = 42/109 (38%), Positives = 68/109 (62%), Gaps = 11/109 (10%) Query: 6 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEK--QIDVETVGERNAEYEQFLEDLQKG 63 ASG+ VSD CK + E+K + +R++VF I DEK Q+ ++ +G YE F + + Sbjct: 5 ASGMHVSDECKLKFLELKAKRNYRFIVFKI-DEKAQQVMIDKLGNPEETYEDFTRSIPED 63 Query: 64 GTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 ECRY ++D+++T T E +K K+F ++W PDT++V+ KMLY+ Sbjct: 64 ---ECRYAVYDYDFT-----TPENCQKSKIFFIAWSPDTSRVRSKMLYA 104 >At2g31200.1 68415.m03810 actin-depolymerizing factor 6 (ADF6) identical to SP|Q9ZSK2 Actin-depolymerizing factor 6 (ADF-6) (AtADF6) {Arabidopsis thaliana} Length = 146 Score = 83.4 bits (197), Expect = 3e-17 Identities = 42/107 (39%), Positives = 64/107 (59%), Gaps = 9/107 (8%) Query: 7 SGVTVSDACKTTYEEIKKDKKHRYVVFYI-RDEKQIDVETVGERNAEYEQFLEDLQKGGT 65 SG+ V+D KTT+ E+++ K HRYVVF I +K++ VE G Y+ FL L Sbjct: 13 SGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLP---D 69 Query: 66 GECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 +CRY ++DF++ TSE +K K+F +W P T+ ++ K+LYS Sbjct: 70 NDCRYAVYDFDFV-----TSENCQKSKIFFFAWSPSTSGIRAKVLYS 111 >At4g00680.1 68417.m00093 actin-depolymerizing factor, putative strong similarity to SP|P30175 Actin-depolymerizing factor (ADF) {Lilium longiflorum}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 140 Score = 83.0 bits (196), Expect = 4e-17 Identities = 41/109 (37%), Positives = 67/109 (61%), Gaps = 11/109 (10%) Query: 6 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEK--QIDVETVGERNAEYEQFLEDLQKG 63 ASG+ V+D CK + E+K + +R++VF I DEK Q+ +E +G Y+ F + Sbjct: 5 ASGMHVNDECKIKFLELKAKRTYRFIVFKI-DEKAQQVQIEKLGNPEETYDDFTSSIPDD 63 Query: 64 GTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 ECRY ++DF++T T + +K K+F ++W PDT++V+ KMLY+ Sbjct: 64 ---ECRYAVYDFDFT-----TEDNCQKSKIFFIAWSPDTSRVRSKMLYA 104 >At3g46000.1 68416.m04977 actin-depolymerizing factor, putative (ADF2) strong similarity to SP|Q9ZSK3 Actin-depolymerizing factor 4 (ADF-4) (AtADF4) {Arabidopsis thaliana}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 137 Score = 83.0 bits (196), Expect = 4e-17 Identities = 42/107 (39%), Positives = 64/107 (59%), Gaps = 9/107 (8%) Query: 6 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGT 65 ASG+ V D CK + E+K + R +V+ I D KQ+ VE +GE Y+ F L Sbjct: 5 ASGMAVHDDCKLKFMELKAKRTFRTIVYKIED-KQVIVEKLGEPEQSYDDFAASLP---A 60 Query: 66 GECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 +CRY ++DF++ T+E +K K+F ++W PDTAKV+ KM+Y+ Sbjct: 61 DDCRYCIYDFDFV-----TAENCQKSKIFFIAWSPDTAKVRDKMIYA 102 >At5g52360.1 68418.m06497 actin-depolymerizing factor, putative strong similarity to pollen specific actin-depolymerizing factor 2 [Nicotiana tabacum] GI:22857914; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 137 Score = 81.8 bits (193), Expect = 1e-16 Identities = 39/107 (36%), Positives = 66/107 (61%), Gaps = 9/107 (8%) Query: 6 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGT 65 ASG+ V D CK + E+K + +R+++F I D +Q+ VE +G Y+ F L Sbjct: 5 ASGMAVEDECKLKFLELKAKRNYRFIIFRI-DGQQVVVEKLGSPQENYDDFTNYLPPN-- 61 Query: 66 GECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 ECRY ++DF++T T+E +K K+F ++W PD+++V+ KM+Y+ Sbjct: 62 -ECRYAVYDFDFT-----TAENIQKSKIFFIAWSPDSSRVRMKMVYA 102 >At4g25590.1 68417.m03687 actin-depolymerizing factor, putative strong similarity to pollen specific actin-depolymerizing factor 2 [Nicotiana tabacum] GI:22857914; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 130 Score = 76.6 bits (180), Expect = 4e-15 Identities = 36/104 (34%), Positives = 62/104 (59%), Gaps = 9/104 (8%) Query: 9 VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGEC 68 + V D CK + E+K + +R+++F I D +Q+ VE +G + Y+ F L EC Sbjct: 1 MAVEDECKLKFLELKSKRNYRFIIFRI-DGQQVVVEKLGNPDETYDDFTASLP---ANEC 56 Query: 69 RYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 RY +FDF++ T E +K K+F ++W PD+++V+ KM+Y+ Sbjct: 57 RYAVFDFDFI-----TDENCQKSKIFFIAWSPDSSRVRMKMVYA 95 >At5g59880.2 68418.m07509 actin-depolymerizing factor 3 (ADF3) identical to SP|Q9ZSK4 Actin-depolymerizing factor 3 (ADF 3) (AtADF3) {Arabidopsis thaliana} Length = 124 Score = 73.7 bits (173), Expect = 3e-14 Identities = 35/100 (35%), Positives = 58/100 (58%), Gaps = 9/100 (9%) Query: 6 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGG 64 ASG+ V D CK + E+K + HR++++ I + +KQ+ VE +GE +E L Sbjct: 5 ASGMAVHDDCKLKFMELKTKRTHRFIIYKIEELQKQVIVEKIGEPGQTHEDLAASLP--- 61 Query: 65 TGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAK 104 ECRY +FDF++ +SE + ++F ++W PDTA+ Sbjct: 62 ADECRYAIFDFDFV-----SSEGVPRSRIFFVAWSPDTAR 96 >At2g16700.1 68415.m01916 actin-depolymerizing factor 5 (ADF5) identical to SP|Q9ZNT3 Actin-depolymerizing factor 5 (ADF-5) (AtADF5) {Arabidopsis thaliana} Length = 143 Score = 72.9 bits (171), Expect = 5e-14 Identities = 33/110 (30%), Positives = 65/110 (59%), Gaps = 9/110 (8%) Query: 4 LEASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDE-KQIDVETVGERNAEYEQFLEDLQK 62 + +G+ V+D C +++ ++K K HRY+VF I ++ +++ V+ VG Y + L Sbjct: 7 MATTGMRVTDECTSSFMDMKWKKVHRYIVFKIEEKSRKVTVDKVGGAGESYHDLEDSLP- 65 Query: 63 GGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 +CRY +FDF++ T + +K K+F ++W P+ +K++ K+LY+ Sbjct: 66 --VDDCRYAVFDFDFV-----TVDNCRKSKIFFIAWSPEASKIRAKILYA 108 >At4g34970.1 68417.m04957 actin-depolymerizing factor, putative similar to SP|Q9ZNT3 Actin-depolymerizing factor 5 (ADF-5) (AtADF5) {Arabidopsis thaliana}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 130 Score = 70.1 bits (164), Expect = 3e-13 Identities = 31/103 (30%), Positives = 62/103 (60%), Gaps = 9/103 (8%) Query: 11 VSDACKTTYEEIKKDKKHRYVVFYIRDE-KQIDVETVGERNAEYEQFLEDLQKGGTGECR 69 ++D CK ++ E+K K HRYVV+ + ++ +++ V+ VG Y+ L + +CR Sbjct: 1 MTDDCKKSFMEMKWKKVHRYVVYKLEEKSRKVTVDKVGAAGESYDDLAASLPE---DDCR 57 Query: 70 YGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 Y +FDF+Y T + + K+F ++W P+ +++++KM+Y+ Sbjct: 58 YAVFDFDYV-----TVDNCRMSKIFFITWSPEASRIREKMMYA 95 >At3g45990.1 68416.m04976 actin-depolymerizing factor, putative similar to SP|Q9ZSK3 Actin-depolymerizing factor 4 (ADF-4) (AtADF4) {Arabidopsis thaliana}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 133 Score = 63.3 bits (147), Expect = 4e-11 Identities = 37/110 (33%), Positives = 57/110 (51%), Gaps = 18/110 (16%) Query: 9 VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETV------GERNAEYEQFLEDLQK 62 + + D CK T+ E+K+ + R +V+ I D Q+ VE GER YE+F L Sbjct: 1 MVLHDDCKLTFLELKERRTFRSIVYKIEDNMQVIVEKHHYKKMHGEREQSYEEFANSLP- 59 Query: 63 GGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYS 112 ECRY + D E+ ++K+ ++W P TAK++KKM+YS Sbjct: 60 --ADECRYAILDIEFV---------PGERKICFIAWSPSTAKMRKKMIYS 98 >At3g03290.1 68416.m00326 universal stress protein (USP) family protein contains Pfam profile: PF00582 universal stress protein family Length = 274 Score = 31.5 bits (68), Expect = 0.14 Identities = 12/24 (50%), Positives = 18/24 (75%) Query: 44 ETVGERNAEYEQFLEDLQKGGTGE 67 ET G+ +EYE+F E+ ++GG GE Sbjct: 45 ETDGDSTSEYEEFEEEKERGGEGE 68 >At1g21630.1 68414.m02708 calcium-binding EF hand family protein contains INTERPRO:IPR002048 calcium-binding EF-hand domain; ESTs gb|T44428 and gb|AA395440 come from this gene Length = 1218 Score = 28.7 bits (61), Expect = 0.96 Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 4/67 (5%) Query: 7 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 66 S +T+ +A T+ +I++ K Y +E ++D V ER + LE+L K Sbjct: 676 SKLTIEEA---TFRDIQEKKMELYQAIVKFEEGKLDDSIVKERTEHIQSGLEELIKNLNE 732 Query: 67 ECR-YGL 72 C+ YG+ Sbjct: 733 RCKQYGV 739 >At1g71270.1 68414.m08225 Vps52/Sac2 family protein similar to SP|P39904 SAC2 protein {Saccharomyces cerevisiae}; contains Pfam profile PF04129: Vps52 / Sac2 family Length = 707 Score = 26.6 bits (56), Expect = 3.9 Identities = 16/41 (39%), Positives = 21/41 (51%) Query: 22 IKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQK 62 + + K ++V I K IDV GE N EY + LE L K Sbjct: 155 VAESKLAKFVEDIIVPPKMIDVIVDGEVNEEYMKTLEILSK 195 >At1g44835.1 68414.m05136 YbaK/prolyl-tRNA synthetase family protein contains Pfam PF04073: YbaK / prolyl-tRNA synthetases associated domain; similar to 25.7 kDa protein (GI:7271117) [Cicer arietinum] Length = 307 Score = 26.6 bits (56), Expect = 3.9 Identities = 9/26 (34%), Positives = 17/26 (65%) Query: 24 KDKKHRYVVFYIRDEKQIDVETVGER 49 KDKKHRY + + ++D++ + +R Sbjct: 51 KDKKHRYYIVSAMVDTKVDMKVLSQR 76 >At2g18630.1 68415.m02169 expressed protein unusual splice site at second intron; GA instead of conserved GT at donor site; similar to At14a GI:11994571 and GI:11994573 [Arabidopsis thaliana] Length = 393 Score = 25.8 bits (54), Expect = 6.8 Identities = 23/97 (23%), Positives = 46/97 (47%), Gaps = 9/97 (9%) Query: 3 FLEASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQK 62 + E++ T+ D C + + ++ + ++ + + Q + E + N +YE+ LE+L++ Sbjct: 117 YFESTAKTM-DFCSELENCLNRARRSQVIIQFAVN--QFEEENEDKENRKYEKTLEELKR 173 Query: 63 ---GGTGECR--YGLFDFEYTHQCQGTSEASK-KQKL 93 G + + LFD Y Q E K K+KL Sbjct: 174 FKVAGEPFTKEFFALFDLVYKQQVMMLEELHKLKRKL 210 >At5g61560.1 68418.m07725 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 796 Score = 25.4 bits (53), Expect = 9.0 Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 1/56 (1%) Query: 38 EKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEASKKQKL 93 E++++ E E + +Q LED +GG + R FE+ + TS S + K+ Sbjct: 381 EERLEAEARAEEVRKEKQRLEDALEGGPLQ-RQQYMKFEWEEIVEATSSFSDELKI 435 >At2g45650.1 68415.m05676 MADS-box protein (AGL6) Length = 252 Score = 25.4 bits (53), Expect = 9.0 Identities = 10/33 (30%), Positives = 17/33 (51%) Query: 76 EYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKK 108 E ++C S ++ K + SWC + K+K K Sbjct: 67 ERYNRCYNCSLSNNKPEETTQSWCQEVTKLKSK 99 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.317 0.133 0.392 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,673,911 Number of Sequences: 28952 Number of extensions: 103696 Number of successful extensions: 313 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 262 Number of HSP's gapped (non-prelim): 21 length of query: 113 length of database: 12,070,560 effective HSP length: 72 effective length of query: 41 effective length of database: 9,986,016 effective search space: 409426656 effective search space used: 409426656 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 53 (25.4 bits)
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