Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000124-TA|BGIBMGA000124-PA|IPR002048|Calcium-binding
EF-hand
         (103 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    45   2e-06
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    31   0.048
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    29   0.15 
SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces p...    27   0.78 
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc...    26   1.0  
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha...    25   3.1  
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate...    23   9.6  
SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit...    23   9.6  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 45.2 bits (102), Expect = 2e-06
 Identities = 26/84 (30%), Positives = 42/84 (50%), Gaps = 5/84 (5%)

Query: 18  GCCPTEAEIQEVILATENKEATGNVPLTNFLPYMCKVMIEHRFYPASAEMLLAAFRYFDT 77
           G  PT AE+Q++I   +  +  G +  T FL  M + M +      + E +  AF+ FD 
Sbjct: 42  GQSPTAAELQDMINEVD-ADGNGTIDFTEFLTMMARKMKD----TDNEEEVREAFKVFDK 96

Query: 78  EGRGYLTKERFAKLMMEEGESFTQ 101
           +G GY+T E    ++   GE  +Q
Sbjct: 97  DGNGYITVEELTHVLTSLGERLSQ 120


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 30.7 bits (66), Expect = 0.048
 Identities = 14/37 (37%), Positives = 19/37 (51%)

Query: 64  SAEMLLAAFRYFDTEGRGYLTKERFAKLMMEEGESFT 100
           S E  + AFR FD +  GY+   +FA  M   GE  +
Sbjct: 76  SEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLS 112


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 29.1 bits (62), Expect = 0.15
 Identities = 13/35 (37%), Positives = 19/35 (54%)

Query: 61  YPASAEMLLAAFRYFDTEGRGYLTKERFAKLMMEE 95
           + A    +L   R FD  G+GYL  E F ++M E+
Sbjct: 68  FNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEK 102


>SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 704

 Score = 26.6 bits (56), Expect = 0.78
 Identities = 18/70 (25%), Positives = 34/70 (48%), Gaps = 1/70 (1%)

Query: 22  TEAEIQEVILATENKEATGNVPLTNFLPYMCKVMIEHRFYPASAEMLLAAFRYFDTEGRG 81
           +E +I+E  LA+ ++  + +  + NF+  M   +++H  YP+S ++L       D     
Sbjct: 297 SEKQIEEEPLASADETLSQSADIDNFMFDMDTGLLQHT-YPSSVQILFQEDSVADDLTPC 355

Query: 82  YLTKERFAKL 91
           Y TK     L
Sbjct: 356 YSTKNSTCNL 365


>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1679

 Score = 26.2 bits (55), Expect = 1.0
 Identities = 10/20 (50%), Positives = 16/20 (80%)

Query: 39   TGNVPLTNFLPYMCKVMIEH 58
            TGN  LT+F+P + +VM+E+
Sbjct: 1195 TGNSTLTSFVPAIMQVMLEY 1214


>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
           Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 884

 Score = 24.6 bits (51), Expect = 3.1
 Identities = 11/28 (39%), Positives = 16/28 (57%)

Query: 65  AEMLLAAFRYFDTEGRGYLTKERFAKLM 92
           +E L   +   DT+ RGY+ K+ FA  M
Sbjct: 335 SEELARIWDTVDTQDRGYIDKDEFAVAM 362


>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
           transferase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 453

 Score = 23.0 bits (47), Expect = 9.6
 Identities = 16/46 (34%), Positives = 20/46 (43%), Gaps = 3/46 (6%)

Query: 20  CPTEAEIQEVIL---ATENKEATGNVPLTNFLPYMCKVMIEHRFYP 62
           CP E   Q++ L   +T N     N  LT+ LP    V     FYP
Sbjct: 261 CPEEQLEQKISLLLSSTRNSPTMSNSSLTHLLPTPIFVGDVTGFYP 306


>SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit
          Rpb4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 135

 Score = 23.0 bits (47), Expect = 9.6
 Identities = 10/28 (35%), Positives = 18/28 (64%), Gaps = 1/28 (3%)

Query: 22 TEAEIQ-EVILATENKEATGNVPLTNFL 48
          +EA+I  E +LA   +E  G +P+T+ +
Sbjct: 28 SEAKILIETVLAQRARETNGEIPMTDVM 55


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.323    0.137    0.407 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 457,307
Number of Sequences: 5004
Number of extensions: 16059
Number of successful extensions: 44
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 36
Number of HSP's gapped (non-prelim): 12
length of query: 103
length of database: 2,362,478
effective HSP length: 63
effective length of query: 40
effective length of database: 2,047,226
effective search space: 81889040
effective search space used: 81889040
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 47 (23.0 bits)