GeneModel BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000119-TA|BGIBMGA000119-PA|IPR000860|Porphobilinogen
deaminase
         (407 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g08280.1 68418.m00975 hydroxymethylbilane synthase / porphobi...   202   3e-52
At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferlin...    32   0.79 
At4g28410.1 68417.m04067 aminotransferase-related similar to nic...    30   3.2  
At1g24190.1 68414.m03051 paired amphipathic helix repeat-contain...    29   4.2  
At5g11500.1 68418.m01342 expressed protein contains Pfam profile...    29   7.4  

>At5g08280.1 68418.m00975 hydroxymethylbilane synthase /
           porphobilinogen deaminase, chloroplast /
           pre-uroporphyrinogen synthase identical to SP|Q43316
          Length = 382

 Score =  202 bits (494), Expect = 3e-52
 Identities = 114/283 (40%), Positives = 170/283 (60%), Gaps = 15/283 (5%)

Query: 9   IHVGSRKSELALVQTNFVIDSLKRNYPEK----EFKIVTMTTLGDRVLDQPLPKIGEKSL 64
           I +G+R S LAL Q     + LK+ +PE        I  + T GD++L QPL  IG K L
Sbjct: 75  IRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGL 134

Query: 65  FTKDLEDALMSKNVDFVVHSLKDLPTTLPDGLVIGAVFEREDPRDALVLNEKFKEYSLST 124
           FTK++++AL++ ++D  VHS+KD+PT LP+  ++     RED RDA +        +L+ 
Sbjct: 135 FTKEIDEALINGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFIC---LTAATLAE 191

Query: 125 LPSGSVIGTSSLRRTAQLNGNYPQLKVVD-VRGNLNTRLRKLDDEDGKYSDLILASAGLS 183
           LP+GSV+GT+SLRR +Q+   YP L V +  RGN+ TRL KL  + GK    +LA AGL 
Sbjct: 192 LPAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKL--QGGKVQATLLALAGLK 249

Query: 184 RMGWGNRMSKVLPCSEMLYAVGQGALAVECRADNEEVLAMLAPFNHPETYCRVLAERSFL 243
           R+     ++ +L   EML AV QGA+ + CR D++++   LA  NH ET   +  ER+FL
Sbjct: 250 RLSMTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFL 309

Query: 244 KTLGGGCSAPV-GISTKLKQNDNVYKLTLTGAVWSLDGSTKLE 285
           +TL G C  P+ G ++K ++ + +++    G V S DG+  LE
Sbjct: 310 ETLDGSCRTPIAGYASKDEEGNCIFR----GLVASPDGTKVLE 348


>At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferling
           (PFI) almost identical to tubulin folding cofactor E
           (Pfifferling; PFI) GI:20514267 from [Arabidopsis
           thaliana]; identical to cDNA tubulin folding cofactor E,
           GI:20514266
          Length = 531

 Score = 31.9 bits (69), Expect = 0.79
 Identities = 16/46 (34%), Positives = 25/46 (54%)

Query: 113 LNEKFKEYSLSTLPSGSVIGTSSLRRTAQLNGNYPQLKVVDVRGNL 158
           + +K   +   T  S S +G SSL  ++ L    P LK++D+ GNL
Sbjct: 126 IQDKLSRFEELTSASLSYLGVSSLGVSSDLGSILPNLKLLDLTGNL 171


>At4g28410.1 68417.m04067 aminotransferase-related similar to
           nicotianamine aminotransferase [Hordeum vulgare subsp.
           vulgare] GI:6469090
          Length = 447

 Score = 29.9 bits (64), Expect = 3.2
 Identities = 17/42 (40%), Positives = 27/42 (64%), Gaps = 5/42 (11%)

Query: 51  VLDQPLPKIGEKS---LFTKDLEDALMSKNVDFVVHSLKDLP 89
           +L   LP I EK+    F K+  ++++S+NVDF   +LKD+P
Sbjct: 321 ILQFALPNILEKTKKEFFEKN--NSILSQNVDFAFDALKDIP 360


>At1g24190.1 68414.m03051 paired amphipathic helix repeat-containing
           protein similar to transcription co-repressor Sin3
           [Xenopus laevis] GI:4960210; contains Pfam profile
           PF02671: Paired amphipathic helix repeat
          Length = 1353

 Score = 29.5 bits (63), Expect = 4.2
 Identities = 14/57 (24%), Positives = 27/57 (47%), Gaps = 1/57 (1%)

Query: 288 LDQTFGQIRKTVKHKLSPTEEASSKKIKTDKTVEADNEIAVLNRRITDKT-GDLGCE 343
           LD     +  T KH      + +S ++KT+  +  ++ +  LN R  ++  GD G +
Sbjct: 518 LDMLLESVNSTTKHVEELLTKINSNELKTNSPIRVEDHLTALNLRCIERLYGDHGLD 574


>At5g11500.1 68418.m01342 expressed protein contains Pfam profile
           PF05670: Domain of unknown function (DUF814)
          Length = 215

 Score = 28.7 bits (61), Expect = 7.4
 Identities = 18/62 (29%), Positives = 31/62 (50%), Gaps = 1/62 (1%)

Query: 292 FGQIRKTVKHKLSPTEEASSKKIKTDKTVEADNEIAV-LNRRITDKTGDLGCEDLAPNVA 350
           +  ++KT    +      +SK ++T +  +  NEI   LN+   ++T DL  E  A N A
Sbjct: 94  WSNLKKTASMDVGQVGFHNSKMVRTIRVEKRVNEIVNRLNKTKVERTPDLRAEREAVNAA 153

Query: 351 DR 352
           +R
Sbjct: 154 ER 155


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.315    0.134    0.378 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,354,918
Number of Sequences: 28952
Number of extensions: 401744
Number of successful extensions: 1063
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1058
Number of HSP's gapped (non-prelim): 5
length of query: 407
length of database: 12,070,560
effective HSP length: 83
effective length of query: 324
effective length of database: 9,667,544
effective search space: 3132284256
effective search space used: 3132284256
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 60 (28.3 bits)