Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000112-TA|BGIBMGA000112-PA|undefined
(100 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_2537| Best HMM Match : zf-C2HC5 (HMM E-Value=2.5e-28) 29 0.86
SB_56291| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 1.1
SB_20374| Best HMM Match : zf-U1 (HMM E-Value=1.7) 28 1.1
SB_51937| Best HMM Match : zf-CCHC (HMM E-Value=0.00067) 27 2.0
SB_49539| Best HMM Match : RVT_1 (HMM E-Value=1.8e-39) 27 2.0
SB_48126| Best HMM Match : RVT_1 (HMM E-Value=5.5e-39) 27 2.0
SB_39101| Best HMM Match : RVT_1 (HMM E-Value=2.4e-38) 27 2.0
SB_30649| Best HMM Match : zf-C2H2 (HMM E-Value=1.7e-24) 27 2.0
SB_25140| Best HMM Match : RVT_1 (HMM E-Value=7.8e-38) 27 2.0
SB_15135| Best HMM Match : RVT_1 (HMM E-Value=8.4e-39) 27 2.0
SB_9368| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 2.0
SB_155| Best HMM Match : zf-CCHC (HMM E-Value=0.00067) 27 2.0
SB_46071| Best HMM Match : RVP (HMM E-Value=3e-05) 27 2.0
SB_45108| Best HMM Match : RVT_1 (HMM E-Value=4.9e-37) 27 2.0
SB_28983| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 2.0
SB_28642| Best HMM Match : RVT_1 (HMM E-Value=8.4e-39) 27 2.0
SB_23675| Best HMM Match : zf-CCHC (HMM E-Value=0.00067) 27 2.0
SB_19158| Best HMM Match : zf-CCHC (HMM E-Value=0.00067) 27 2.0
SB_15324| Best HMM Match : RVP (HMM E-Value=8.1e-05) 27 2.0
SB_10983| Best HMM Match : RVP (HMM E-Value=3.8e-05) 27 2.0
SB_2341| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 3.5
SB_39371| Best HMM Match : Lectin_C (HMM E-Value=1.4e-06) 26 4.6
SB_25065| Best HMM Match : No HMM Matches (HMM E-Value=.) 26 4.6
SB_31380| Best HMM Match : zf-CCHC (HMM E-Value=4.6e-05) 26 6.1
SB_59028| Best HMM Match : zf-C3HC4 (HMM E-Value=1.4e-05) 25 8.0
SB_58580| Best HMM Match : zf-AD (HMM E-Value=0.29) 25 8.0
SB_55440| Best HMM Match : No HMM Matches (HMM E-Value=.) 25 8.0
SB_37786| Best HMM Match : No HMM Matches (HMM E-Value=.) 25 8.0
SB_29995| Best HMM Match : zf-C3HC4 (HMM E-Value=1.4e-05) 25 8.0
SB_1181| Best HMM Match : DUF1604 (HMM E-Value=0) 25 8.0
SB_50665| Best HMM Match : No HMM Matches (HMM E-Value=.) 25 8.0
SB_43654| Best HMM Match : zf-AD (HMM E-Value=0.2) 25 8.0
SB_38368| Best HMM Match : zf-C3HC4 (HMM E-Value=4.4e-15) 25 8.0
SB_22842| Best HMM Match : No HMM Matches (HMM E-Value=.) 25 8.0
SB_20896| Best HMM Match : TSP_1 (HMM E-Value=4.8e-28) 25 8.0
SB_14987| Best HMM Match : TPR_2 (HMM E-Value=0.0065) 25 8.0
SB_8757| Best HMM Match : zf-C2H2 (HMM E-Value=0) 25 8.0
>SB_2537| Best HMM Match : zf-C2HC5 (HMM E-Value=2.5e-28)
Length = 523
Score = 28.7 bits (61), Expect = 0.86
Identities = 15/71 (21%), Positives = 29/71 (40%), Gaps = 3/71 (4%)
Query: 24 RNTVESGGRVVGENKALTAKKGRFNPYTSTFQQCKICRTKVHQVGSHYCQACAYKKGI-- 81
+ T + G + K + +K + + + + R+ V G H C+ K G+
Sbjct: 85 KETSPTKGNSKADKKIVEQEKKKSTKFVPLYSKEGEARSSVRLPGRHPCECLGQKHGLVN 144
Query: 82 -CAMCGKKILD 91
C CG+ + D
Sbjct: 145 NCTSCGRIVCD 155
>SB_56291| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 434
Score = 28.3 bits (60), Expect = 1.1
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Query: 12 RVITPDPWKAGARNTVESGGRVVGENKALTAKK 44
RV+T + W AG VE ++GEN A T +K
Sbjct: 316 RVVTANVWTAGTNANVEMD--IIGENGAETGEK 346
>SB_20374| Best HMM Match : zf-U1 (HMM E-Value=1.7)
Length = 783
Score = 28.3 bits (60), Expect = 1.1
Identities = 13/54 (24%), Positives = 23/54 (42%), Gaps = 1/54 (1%)
Query: 35 GENKALTAKKGRFNPYTSTFQQCKICRTKVHQVGSHYCQACAYKKGICAMCGKK 88
G + K F+ + +QC +C + + C+AC K +C C +K
Sbjct: 232 GSKSGASHIKDMFSKVLGSTEQCLMCLQNDKRC-TFLCEACYNDKDVCDQCSEK 284
>SB_51937| Best HMM Match : zf-CCHC (HMM E-Value=0.00067)
Length = 151
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 106 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 134
>SB_49539| Best HMM Match : RVT_1 (HMM E-Value=1.8e-39)
Length = 1311
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 106 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 134
>SB_48126| Best HMM Match : RVT_1 (HMM E-Value=5.5e-39)
Length = 1510
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 208 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 236
>SB_39101| Best HMM Match : RVT_1 (HMM E-Value=2.4e-38)
Length = 856
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 351 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 379
>SB_30649| Best HMM Match : zf-C2H2 (HMM E-Value=1.7e-24)
Length = 463
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/62 (20%), Positives = 28/62 (45%)
Query: 38 KALTAKKGRFNPYTSTFQQCKICRTKVHQVGSHYCQACAYKKGICAMCGKKILDTKNYRQ 97
KA T P+ T++ C+ + ++ HY K+ +C +C K+ + + + +
Sbjct: 382 KAHTRTHTGERPFMCTWKDCEKRFARSDELARHYRTHTGEKRYVCKICDKRFMRSDHLSK 441
Query: 98 SS 99
S
Sbjct: 442 HS 443
>SB_25140| Best HMM Match : RVT_1 (HMM E-Value=7.8e-38)
Length = 1425
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 877 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 905
>SB_15135| Best HMM Match : RVT_1 (HMM E-Value=8.4e-39)
Length = 794
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 343 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 371
>SB_9368| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 874
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 423 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 451
>SB_155| Best HMM Match : zf-CCHC (HMM E-Value=0.00067)
Length = 296
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 208 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 236
>SB_46071| Best HMM Match : RVP (HMM E-Value=3e-05)
Length = 403
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 208 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 236
>SB_45108| Best HMM Match : RVT_1 (HMM E-Value=4.9e-37)
Length = 1122
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 224 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 252
>SB_28983| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 951
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 494 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 522
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 547 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 575
>SB_28642| Best HMM Match : RVT_1 (HMM E-Value=8.4e-39)
Length = 1750
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 186 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 214
>SB_23675| Best HMM Match : zf-CCHC (HMM E-Value=0.00067)
Length = 342
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 181 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 209
>SB_19158| Best HMM Match : zf-CCHC (HMM E-Value=0.00067)
Length = 369
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 208 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 236
>SB_15324| Best HMM Match : RVP (HMM E-Value=8.1e-05)
Length = 415
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 45 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 73
>SB_10983| Best HMM Match : RVP (HMM E-Value=3.8e-05)
Length = 717
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Query: 61 RTKVHQVGS--HYCQACAYKKGICAMCGK 87
+T H+ GS H ++C YKK C CGK
Sbjct: 247 KTACHRCGSAEHDGKSCKYKKYKCDNCGK 275
>SB_2341| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 731
Score = 26.6 bits (56), Expect = 3.5
Identities = 13/41 (31%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Query: 23 ARNTVESGGRVVGENK--ALTAKKGRFNPYTSTFQQCKICR 61
A+N + +V+ E+ +T K F+P ++T ++ KICR
Sbjct: 164 AKNVSKKNVKVLDEDTYVQVTPDKQEFSPKSNTLEKSKICR 204
>SB_39371| Best HMM Match : Lectin_C (HMM E-Value=1.4e-06)
Length = 490
Score = 26.2 bits (55), Expect = 4.6
Identities = 12/34 (35%), Positives = 19/34 (55%)
Query: 28 ESGGRVVGENKALTAKKGRFNPYTSTFQQCKICR 61
ES GR + + +T +K F+P +T + KI R
Sbjct: 439 ESAGRYLKTRREVTPEKLGFSPQNNTLEMSKIWR 472
>SB_25065| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 161
Score = 26.2 bits (55), Expect = 4.6
Identities = 10/28 (35%), Positives = 14/28 (50%)
Query: 51 TSTFQQCKICRTKVHQVGSHYCQACAYK 78
TS C C TK+ + + +C AC K
Sbjct: 9 TSVAMFCHCCGTKISEENTKFCFACGEK 36
>SB_31380| Best HMM Match : zf-CCHC (HMM E-Value=4.6e-05)
Length = 1082
Score = 25.8 bits (54), Expect = 6.1
Identities = 20/63 (31%), Positives = 25/63 (39%), Gaps = 12/63 (19%)
Query: 26 TVESGGRVVGE-NKALTAKKGRFNPYTSTFQQCKICRTKVHQVGSHYCQACAYKKGICAM 84
T E + G NK TA+KG N + +CK G HY C +K C
Sbjct: 192 TTEKASSLQGTVNKLGTARKG--NTEEPSCYRCK---------GKHYASNCKFKLAKCFK 240
Query: 85 CGK 87
C K
Sbjct: 241 CSK 243
>SB_59028| Best HMM Match : zf-C3HC4 (HMM E-Value=1.4e-05)
Length = 362
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 15 KGLCRVCGKRLKKAKSLYAQS 35
>SB_58580| Best HMM Match : zf-AD (HMM E-Value=0.29)
Length = 233
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 42 KGLCRVCGKRLKKAKSLYAQS 62
>SB_55440| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 382
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 15 KGLCRVCGKRLKKAKSLYAQS 35
>SB_37786| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 434
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 19 KGLCRVCGKRLKKAKSLYAQS 39
>SB_29995| Best HMM Match : zf-C3HC4 (HMM E-Value=1.4e-05)
Length = 362
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 15 KGLCRVCGKRLKKAKSLYAQS 35
>SB_1181| Best HMM Match : DUF1604 (HMM E-Value=0)
Length = 1035
Score = 25.4 bits (53), Expect = 8.0
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Query: 24 RNTVESGGRVVGENKALTAKKGRFNPYTSTFQQCKICRTK 63
R T E G+++GE T KK F+ Y S Q +I +K
Sbjct: 352 RLTAEERGKILGEEALPTPKKSVFD-YISKEDQSRIKSSK 390
>SB_50665| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 625
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 15 KGLCRVCGKRLKKAKSLYAQS 35
>SB_43654| Best HMM Match : zf-AD (HMM E-Value=0.2)
Length = 284
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 118 KGLCRVCGKRLKKAKSLYAQS 138
>SB_38368| Best HMM Match : zf-C3HC4 (HMM E-Value=4.4e-15)
Length = 846
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 163 KGLCRVCGKRLKKAKSIYAQS 183
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 489 KGLCRVCGKRLKKAKSIYAQS 509
>SB_22842| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 757
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Query: 79 KGICAMCGKKILDTKN-YRQS 98
KG+C +CGK++ K+ Y QS
Sbjct: 166 KGLCRVCGKRLKKAKSLYAQS 186
>SB_20896| Best HMM Match : TSP_1 (HMM E-Value=4.8e-28)
Length = 588
Score = 25.4 bits (53), Expect = 8.0
Identities = 11/29 (37%), Positives = 13/29 (44%), Gaps = 2/29 (6%)
Query: 60 CRTKVHQVGSHYCQACAYKKGICAM--CG 86
C G YC+ AY+ G C M CG
Sbjct: 360 CNNPRPAYGGKYCEGLAYETGQCGMKSCG 388
>SB_14987| Best HMM Match : TPR_2 (HMM E-Value=0.0065)
Length = 1171
Score = 25.4 bits (53), Expect = 8.0
Identities = 14/59 (23%), Positives = 23/59 (38%)
Query: 21 AGARNTVESGGRVVGENKALTAKKGRFNPYTSTFQQCKICRTKVHQVGSHYCQACAYKK 79
A + +E+G V A+ A++ Q + HQ+ HY Q YK+
Sbjct: 372 AAINHYIEAGCSVKAIEAAIQARQWNKAVQIVELQDDTVAERYYHQIAHHYAQVQEYKE 430
>SB_8757| Best HMM Match : zf-C2H2 (HMM E-Value=0)
Length = 539
Score = 25.4 bits (53), Expect = 8.0
Identities = 13/44 (29%), Positives = 21/44 (47%), Gaps = 3/44 (6%)
Query: 57 CKICRTKVHQVGS---HYCQACAYKKGICAMCGKKILDTKNYRQ 97
C IC Q GS HY + K C+ C K+ +++ + R+
Sbjct: 243 CDICHKTFAQAGSLTIHYRRHSNEKHFKCSYCNKRFVESGDMRK 286
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.320 0.132 0.422
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,759,881
Number of Sequences: 59808
Number of extensions: 137660
Number of successful extensions: 385
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 33
Number of HSP's that attempted gapping in prelim test: 374
Number of HSP's gapped (non-prelim): 44
length of query: 100
length of database: 16,821,457
effective HSP length: 71
effective length of query: 29
effective length of database: 12,575,089
effective search space: 364677581
effective search space used: 364677581
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 53 (25.4 bits)
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