Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000104-TA|BGIBMGA000104-PA|IPR008968|Mu2 adaptin subunit
(AP50) of AP2, IPR001392|Clathrin adaptor complex, medium chain,
IPR011012|Longin-like
         (571 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc...   171   2e-43
SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc...    38   0.003
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac...    33   0.081
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc...    33   0.081
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa...    29   1.3  
SPBC1539.09c |trp1||anthranilate synthase component II|Schizosac...    28   4.0  
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...    27   5.3  
SPAC6G9.03c |mug183||histone chaperone Rtt106-like|Schizosacchar...    27   7.1  
SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizo...    27   9.3  

>SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 240

 Score =  171 bits (416), Expect = 2e-43
 Identities = 78/176 (44%), Positives = 124/176 (70%)

Query: 65  LVLIAATVCTKSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQHTFVETESVRYVYQP 124
           +V++A ++  + GKA++SRQF EM++ R+E LL++FP L++   Q+T VE+++VR+VYQP
Sbjct: 1   MVVLAVSIVNRGGKAIISRQFREMSRVRVESLLSSFPALVSEKSQNTTVESDNVRFVYQP 60

Query: 125 LDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALG 184
           LD+LY++LIT   SNIL+D++TL L S+VV   C  L E E+L  AF +  AFDE  +LG
Sbjct: 61  LDELYIVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLG 120

Query: 185 YRESVNLAQVRSFVEMDSHEEKIYQAVRQTQXXXXXXXXXXXXXXLQRERLEAAKR 240
           YR++V+L Q+++++EM+SHEEKI + V + +              L+ ++ EAA+R
Sbjct: 121 YRDNVSLTQIKTYLEMESHEEKIQEIVSRNKEIEATEERKRRIKQLELQKKEAARR 176


>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 426

 Score = 38.3 bits (85), Expect = 0.003
 Identities = 29/140 (20%), Positives = 63/140 (45%), Gaps = 2/140 (1%)

Query: 69  AATVCTKSGKALVSRQF-VEMTKARIEGLLAAFPKLMTG-GRQHTFVETESVRYVYQPLD 126
           A  V    GK ++SR +  ++  + +E  L    ++    G     +  E + Y+Y   +
Sbjct: 4   AIFVLNLKGKVIISRDYRADIPMSVVEKFLPLKSEVEEEQGFSTPCLTHEGINYIYIHHN 63

Query: 127 KLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGYR 186
            +Y+L ++   S+ +E L  LR  + V  +Y  +L E  + +    +    DEI+  G+ 
Sbjct: 64  DVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEIMDFGFP 123

Query: 187 ESVNLAQVRSFVEMDSHEEK 206
           ++     ++ ++   S+  K
Sbjct: 124 QTTETKILQEYITQTSNTVK 143


>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 446

 Score = 33.5 bits (73), Expect = 0.081
 Identities = 20/76 (26%), Positives = 34/76 (44%)

Query: 109 QHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLN 168
           +H  V   S  Y+Y   + LY++ IT    N++  LE L    + +  Y  +L E  V +
Sbjct: 43  RHPIVSIGSSTYIYTKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKD 102

Query: 169 QAFNLLFAFDEIVALG 184
               +    DE++  G
Sbjct: 103 NVSFIFELLDEMIDYG 118


>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 143

 Score = 33.5 bits (73), Expect = 0.081
 Identities = 28/128 (21%), Positives = 51/128 (39%), Gaps = 3/128 (2%)

Query: 75  KSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQH---TFVETESVRYVYQPLDKLYML 131
           + GK  +S+ +V         L A   +L++   Q     F+E E+ + VY+    LY  
Sbjct: 10  RHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVYRRYAGLYFC 69

Query: 132 LITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGYRESVNL 191
                  N L  LE +  F  ++  +   + E +++   + +    DEI+  G     N 
Sbjct: 70  FCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEIILGGEIGESNK 129

Query: 192 AQVRSFVE 199
             V   +E
Sbjct: 130 KSVLERIE 137


>SPAC23C4.19 |spt5||transcription elongation factor
           Spt5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 990

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 2/60 (3%)

Query: 466 TDVNITIPLPSGNTSVVVHQWEGSYTQKGRNLIWSIPLVSKQQ--KTGSLEFTVTPSIPN 523
           TD N  + L +GN  V + +    YT K   LI     + + +  + GS+     P++PN
Sbjct: 752 TDANARVELHTGNKMVTIPKENLLYTTKTGELISYTEFIERSRGIRPGSISTADGPNVPN 811


>SPBC1539.09c |trp1||anthranilate synthase component
           II|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 759

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 3/72 (4%)

Query: 26  VNHQIGRASDPVKFRSDRVEMRS-KIDKHSGPDCGLFKK--QLVLIAATVCTKSGKALVS 82
           V   + RASDP  F  + + + S +I         L K      L+AA    +SG  L+ 
Sbjct: 501 VGESLMRASDPAAFARELLNLSSSEISNGKKTSTPLVKVCGTRSLLAAKTIVESGGDLIG 560

Query: 83  RQFVEMTKARIE 94
             FVE +K +++
Sbjct: 561 LIFVEKSKRKVD 572


>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 21/96 (21%), Positives = 41/96 (42%), Gaps = 2/96 (2%)

Query: 274 TTPARDTRTVGRSAMKLGSRGTDADSFVSRLRSEGDVTAPVASAAQHDAGKPVPADHKDV 333
           T P  +   +  + M+L   G D   ++ ++  E   T   ++ A+ +  + +      V
Sbjct: 162 TVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYT--FSTTAEREIVRDIKEKLCYV 219

Query: 334 HLRFEERLNLIAGRDGDIQTFELSGLLTLRISNEQF 369
            L FE+ L   A      +++EL     + I NE+F
Sbjct: 220 ALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERF 255


>SPAC6G9.03c |mug183||histone chaperone
          Rtt106-like|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 352

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 14/40 (35%), Positives = 21/40 (52%)

Query: 12 GDSETAGPISRKFSVNHQIGRASDPVKFRSDRVEMRSKID 51
          G  + AG + RK  V H++G+    V   S +  MR K+D
Sbjct: 30 GQVKEAGSLKRKDRVQHELGQLVYGVLNLSFQAPMRKKLD 69


>SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase
           Ppk38|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 650

 Score = 26.6 bits (56), Expect = 9.3
 Identities = 15/62 (24%), Positives = 26/62 (41%), Gaps = 1/62 (1%)

Query: 400 TGVIGLKQAQRPFPMHSDVGVLKWRLATTNDDKLAPLSVNCWPSEGVNGGCDV-NIEYEL 458
           T  + +    +P  +H D+ +    LA  N  KL      C P  G     +   +EY++
Sbjct: 149 TEAVAVMHYLKPPLIHRDLKIENVLLAAPNSYKLCDFGSACHPIPGAKTAAEAKQLEYDI 208

Query: 459 EQ 460
           E+
Sbjct: 209 EK 210


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.317    0.133    0.385 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,365,231
Number of Sequences: 5004
Number of extensions: 96449
Number of successful extensions: 237
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 230
Number of HSP's gapped (non-prelim): 11
length of query: 571
length of database: 2,362,478
effective HSP length: 76
effective length of query: 495
effective length of database: 1,982,174
effective search space: 981176130
effective search space used: 981176130
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 56 (26.6 bits)