Silkworm BLAST Result against nematostella

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000104-TA|BGIBMGA000104-PA|IPR008968|Mu2 adaptin subunit
(AP50) of AP2, IPR001392|Clathrin adaptor complex, medium chain,
IPR011012|Longin-like
         (571 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_31870| Best HMM Match : No HMM Matches (HMM E-Value=.)             482   e-136
SB_45476| Best HMM Match : Clat_adaptor_s (HMM E-Value=0)              34   0.27 
SB_53946| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   1.1  
SB_41629| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   4.4  
SB_2298| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   5.8  
SB_13062| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   7.6  

>SB_31870| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1378

 Score =  482 bits (1189), Expect = e-136
 Identities = 254/491 (51%), Positives = 326/491 (66%), Gaps = 17/491 (3%)

Query: 66  VLIAATVCTKSGKALVSRQFVEMTKARIEGLLAAFPKLMTGG---RQHTFVETESVRYVY 122
           VL+AA +CTK+GKA++SRQFVEMT++RIEGLL+AFPKLMT G   +QHTFVETESVRYVY
Sbjct: 470 VLLAAAICTKNGKAIISRQFVEMTRSRIEGLLSAFPKLMTSGSSVKQHTFVETESVRYVY 529

Query: 123 QPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVA 182
           QPL+KLYMLLITTK SNILEDLETLRLFSRV+PEYC  + E+E+   AF L+FAFDEIVA
Sbjct: 530 QPLEKLYMLLITTKHSNILEDLETLRLFSRVIPEYCRAMEESEIGEHAFELIFAFDEIVA 589

Query: 183 LGYRESVNLAQVRSFVEMDSHEEKIYQAVRQTQXXXXXXXXXXXXXXLQRERLEAAK--- 239
           LGYRE+VNLAQ+R+F EMDSHEEK++QAVRQTQ              LQ  ++ AAK   
Sbjct: 590 LGYRENVNLAQIRTFTEMDSHEEKVFQAVRQTQEREAKEEMKKRAKELQAAKVAAAKGRG 649

Query: 240 -RGQPPRXXXXXXXXXXXXXXXXXXXXXXIAEKIPT-TPARDTRTVGR-SAMKLGSRGTD 296
            R   P                       + +  P   P+  +R  G   AMKLGS+  D
Sbjct: 650 GRVAMPGFGGMGSGSTKSDISSGNMDTVPVIDPTPAPKPSYPSRPAGSGKAMKLGSKSKD 709

Query: 297 ADSFVSRLRSEGD--VTAPVASAAQHDAGKPVPADHKDVHLRFEERLNLIAGRDGDIQTF 354
            DSFV +LRSEG   ++    S AQ  A  PV  +   VHL+ EE++ L AGRDG +Q  
Sbjct: 710 VDSFVDKLRSEGTEVISHKQKSLAQKPAAAPV-VNQSSVHLKTEEKITLTAGRDGGLQNM 768

Query: 355 ELSGLLTLRISNEQFGRIHVHVDNKDSRPLQLQTHPNVDKEAFRSTGVIGLKQAQRPFPM 414
           E+ G++ LRIS+ QF +I + V+N D +  Q+QTHPNVDK++F    ++ LKQA + FP 
Sbjct: 769 EIRGIVLLRISDSQFAQIKLAVENNDDKGFQIQTHPNVDKKSFAQDNILVLKQAGKSFPT 828

Query: 415 HSDVGVLKWRLATTNDDKLAPLSVNCWPSEGVNGGCDVNIEYELEQDHLVLTDVNITIPL 474
           +SD+G+L+WR+ TT D+ L PLS+NCWPSE  +G CDVNIEYEL  D L L DV ITIP+
Sbjct: 829 NSDIGLLRWRMQTT-DESLMPLSINCWPSEN-DGQCDVNIEYELLVDGLELNDVVITIPV 886

Query: 475 PSGNTSVVVHQWEGS--YTQKGRNLIWSIPLVSKQQKTGSLEFTVTPSIPNDFFPLSVTW 532
           P G    VV + +G   Y  K   L W +P++    K+GS+EF++    P DFFP++V++
Sbjct: 887 PHGVGGPVVGEIDGEYHYNHKQSTLGWQVPVIDASNKSGSMEFSIAGQ-PGDFFPVTVSF 945

Query: 533 TSETSLALLTA 543
            S  +   L A
Sbjct: 946 FSSKTYCDLKA 956


>SB_45476| Best HMM Match : Clat_adaptor_s (HMM E-Value=0)
          Length = 141

 Score = 34.3 bits (75), Expect = 0.27
 Identities = 27/128 (21%), Positives = 56/128 (43%), Gaps = 2/128 (1%)

Query: 75  KSGKALVSRQFVEMTKARIEGLLAAFPKLMT-GGRQHT-FVETESVRYVYQPLDKLYMLL 132
           ++GK  +++ ++       + L+     L+T    +HT FVE  + + VY+    LY   
Sbjct: 9   RAGKTRLAKWYMNFDDDEKQKLIEEVHALVTVRDAKHTNFVEFRNFKIVYRRYAGLYFCF 68

Query: 133 ITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGYRESVNLA 192
               + N L  LE +  F  V+ E+   + E +++   + +    DE+   G     + +
Sbjct: 69  CVDVSDNNLYYLEAIHNFVEVLNEFFHNVCELDLVFNFYKVYSVVDEMFLAGELRETSQS 128

Query: 193 QVRSFVEM 200
           +V   + M
Sbjct: 129 KVLKQLNM 136


>SB_53946| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 307

 Score = 32.3 bits (70), Expect = 1.1
 Identities = 17/49 (34%), Positives = 28/49 (57%), Gaps = 3/49 (6%)

Query: 14  SETAGPISRKFSV--NHQ-IGRASDPVKFRSDRVEMRSKIDKHSGPDCG 59
           SE +GP   +FS+  NH+ +   S+P K +S  ++   K+ + SGP  G
Sbjct: 246 SEVSGPQKGQFSLIQNHEKVSEVSEPQKGQSSLIQNHEKVSEVSGPQKG 294


>SB_41629| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 256

 Score = 30.3 bits (65), Expect = 4.4
 Identities = 16/39 (41%), Positives = 23/39 (58%)

Query: 285 RSAMKLGSRGTDADSFVSRLRSEGDVTAPVASAAQHDAG 323
           +SA ++  RG+DADS      S+G ++  V SAA H  G
Sbjct: 59  KSASRMSFRGSDADSDDDDDDSQGGLSGRVGSAAGHRRG 97


>SB_2298| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 169

 Score = 29.9 bits (64), Expect = 5.8
 Identities = 21/72 (29%), Positives = 34/72 (47%)

Query: 110 HTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQ 169
           HT   TE  R + + +DK +   +    +  L+D ET  +   +     V LTETE+ + 
Sbjct: 96  HTKGSTEDERLLKKIVDKAFEEKVALTIARYLKDQETFPVEPSIRITVNVDLTETEIHSA 155

Query: 170 AFNLLFAFDEIV 181
           A  +  A D I+
Sbjct: 156 AQVIKNAADSIL 167


>SB_13062| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 389

 Score = 29.5 bits (63), Expect = 7.6
 Identities = 16/49 (32%), Positives = 27/49 (55%), Gaps = 2/49 (4%)

Query: 83  RQFVEMTKARIEGLLAAFPKLMTGGRQHTFVETESVRYVYQPLDKLYML 131
           R+ +E  K R+E LL+  PK++     H   ET+S   ++  +D+L  L
Sbjct: 33  RRMLEGLKNRLEALLS--PKIVAAFNNHCLDETKSYVKIFTAIDRLDQL 79


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.317    0.133    0.385 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,304,982
Number of Sequences: 59808
Number of extensions: 696775
Number of successful extensions: 1292
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1281
Number of HSP's gapped (non-prelim): 6
length of query: 571
length of database: 16,821,457
effective HSP length: 86
effective length of query: 485
effective length of database: 11,677,969
effective search space: 5663814965
effective search space used: 5663814965
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 63 (29.5 bits)