Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000104-TA|BGIBMGA000104-PA|IPR008968|Mu2 adaptin subunit
(AP50) of AP2, IPR001392|Clathrin adaptor complex, medium chain,
IPR011012|Longin-like
(571 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g05010.1 68418.m00532 clathrin adaptor complexes medium subun... 196 2e-50
At2g17380.1 68415.m02007 clathrin assembly protein AP19 identica... 36 0.096
At4g35410.2 68417.m05030 clathrin adaptor complex small chain fa... 34 0.29
At4g16730.1 68417.m02527 terpene synthase/cyclase family protein... 33 0.51
At1g47830.1 68414.m05324 clathrin coat assembly protein, putativ... 31 2.1
At4g31160.1 68417.m04423 transducin family protein / WD-40 repea... 30 3.6
At4g24550.2 68417.m03519 clathrin adaptor complexes medium subun... 30 3.6
At4g24550.1 68417.m03518 clathrin adaptor complexes medium subun... 30 3.6
At1g22000.1 68414.m02752 F-box family protein contains F-box dom... 30 3.6
At5g13760.1 68418.m01604 expressed protein similar to unknown pr... 30 4.8
At2g19790.1 68415.m02312 clathrin adaptor complex small chain fa... 29 6.3
At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun... 29 6.3
At5g12900.1 68418.m01480 expressed protein 29 8.3
At4g35410.1 68417.m05029 clathrin adaptor complex small chain fa... 29 8.3
>At5g05010.1 68418.m00532 clathrin adaptor complexes medium
subunit-related contains pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 527
Score = 196 bits (479), Expect = 2e-50
Identities = 88/177 (49%), Positives = 131/177 (74%)
Query: 65 LVLIAATVCTKSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQHTFVETESVRYVYQP 124
+V++AA + KSGK +VSR +V+M++ RIEGLLAAFPKL+ G+QHT++ETE+VRYVYQP
Sbjct: 1 MVVLAAAIVVKSGKVIVSRHYVDMSRIRIEGLLAAFPKLVGMGKQHTYIETENVRYVYQP 60
Query: 125 LDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALG 184
++ L++LL+TTK SNILEDL TL L S++VPEY + L E + +F L+FAFDE+++LG
Sbjct: 61 IEALFLLLVTTKQSNILEDLATLTLLSKLVPEYSMSLDEEGISRASFELIFAFDEVISLG 120
Query: 185 YRESVNLAQVRSFVEMDSHEEKIYQAVRQTQXXXXXXXXXXXXXXLQRERLEAAKRG 241
++ESV +AQV+ + EM+SHEEK+++ V Q++ + + ++E K G
Sbjct: 121 HKESVTVAQVKQYCEMESHEEKLHKLVMQSKINDTKDVMKRKANEIDKSKIEKNKPG 177
Score = 161 bits (391), Expect = 1e-39
Identities = 99/294 (33%), Positives = 157/294 (53%), Gaps = 15/294 (5%)
Query: 286 SAMKLGSRGTDADSFVSRLRSEG-DVTAPVASAAQHDAGKPVPADHKDVHLRFEERLNLI 344
S MKLG G + + L++EG DV V Q A P P D L EE+LN+
Sbjct: 241 SGMKLGKSGKN--QLMESLKAEGEDVIEDVKPTGQSKAAAPPPTD--PFTLTVEEKLNVA 296
Query: 345 AGRDGDIQTFELSGLLTLRISNEQFGRIHVHVDNKDSRPLQLQTHPNVDKEAFRSTGVIG 404
RDG + +F++ G L+L+I N++ G + V + ++ + +THPN++++ F + ++G
Sbjct: 297 LRRDGGLSSFDMQGTLSLQILNQEDGFVQVQIATGENPEILFKTHPNINRDMFNNENILG 356
Query: 405 LKQAQRPFPM---HSDVGVLKWRLATTNDDKLAPLSVNCWPSEGVNGGCDVNIEYELEQD 461
LK+ +PFP VG+L+WR+ D+ + PL++NCWPS N V++EYE
Sbjct: 357 LKRPDQPFPTGQGGDGVGLLRWRMQRA-DESMVPLTINCWPSVSGN-ETYVSLEYE-ASS 413
Query: 462 HLVLTDVNITIPLPSGNTSVVVHQWEGSYTQKGRN--LIWSIPLVSKQQKTGSLEFTVTP 519
LT+V I++PLP+ + V Q +G + RN L WSI L+ ++GS+EF V P
Sbjct: 414 MFDLTNVIISVPLPALREAPSVRQCDGEWRYDPRNSVLEWSILLIDNSNRSGSMEFVVPP 473
Query: 520 SIPNDFFPLSVTW--TSETSLALLTAVKVTQAEDGSPVDFSQEISFHADKYEFV 571
+ FFP+SV + TS S +T + + G+ F Q A Y+ +
Sbjct: 474 VDSSVFFPISVQFAATSTYSGLKVTGMIPLRGGGGATPRFVQRTQLIAQNYQVI 527
>At2g17380.1 68415.m02007 clathrin assembly protein AP19 identical
to clathrin assembly protein AP19 GI:2231698 from
[Arabidopsis thaliana]
Length = 161
Score = 35.5 bits (78), Expect = 0.096
Identities = 19/82 (23%), Positives = 36/82 (43%)
Query: 103 LMTGGRQHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLT 162
L G + F+E + VY+ LY + +A N LE LE + + ++ Y +
Sbjct: 40 LNRGPKLCNFIEWRGYKVVYKRYASLYFCMCIDEADNELEVLEIIHHYVEILDRYFGSVC 99
Query: 163 ETEVLNQAFNLLFAFDEIVALG 184
E +++ + DE++ G
Sbjct: 100 ELDLIFNFHKAYYILDELLIAG 121
>At4g35410.2 68417.m05030 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 162
Score = 33.9 bits (74), Expect = 0.29
Identities = 19/82 (23%), Positives = 35/82 (42%)
Query: 103 LMTGGRQHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLT 162
L G + FVE + VY+ LY + + N LE LE + + ++ Y +
Sbjct: 40 LNRGPKLCNFVEWRGYKVVYKRYASLYFCMCIDQEDNELEVLEIIHHYVEILDRYFGSVC 99
Query: 163 ETEVLNQAFNLLFAFDEIVALG 184
E +++ + DE++ G
Sbjct: 100 ELDLIFNFHKAYYILDELLIAG 121
>At4g16730.1 68417.m02527 terpene synthase/cyclase family protein
similar to myrcene/ocimene synthase [GI:9957293];
contains Pfam profile: PF01397 terpene synthase family
Length = 547
Score = 33.1 bits (72), Expect = 0.51
Identities = 25/75 (33%), Positives = 37/75 (49%), Gaps = 2/75 (2%)
Query: 112 FVETESVRYVYQPLDKLYMLL-ITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQA 170
F E E +Y + L K++ML+ I +I LE L LF+ VV ++ V E
Sbjct: 274 FYEPE-FKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKWDVNHVERLPNYMR 332
Query: 171 FNLLFAFDEIVALGY 185
LF ++EI +GY
Sbjct: 333 MCFLFLYNEINQIGY 347
>At1g47830.1 68414.m05324 clathrin coat assembly protein, putative
similar to clathrin coat assembly protein AP17
GB:CAA65533 GI:2959358 from [Zea mays]; contains Pfam
profile: PF01217 clathrin adaptor complex small chain
Length = 142
Score = 31.1 bits (67), Expect = 2.1
Identities = 24/135 (17%), Positives = 58/135 (42%), Gaps = 5/135 (3%)
Query: 75 KSGKALVSRQFVEMTKARIEGLLAAFPKLMTG--GRQHTFVETESVRYVYQPLDKLYMLL 132
+ GK +++ +V + ++ + +L+ + FVE + + +Y+ L+ +
Sbjct: 10 RQGKTRLAKYYVPLEESEKHKVEYEVHRLVVNRDAKFTNFVEFRTHKVIYRRYAGLFFSV 69
Query: 133 ITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGYRESVNLA 192
N L LE++ LF ++ + + E +++ + DE + G + +
Sbjct: 70 CVDITDNELAYLESIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILAGELQETS-- 127
Query: 193 QVRSFVEMDSHEEKI 207
R+ +E S EK+
Sbjct: 128 -KRAIIERMSELEKL 141
>At4g31160.1 68417.m04423 transducin family protein / WD-40 repeat
family protein contains 2 WD-40 repeats (PF00400);
KIAA0800 protein, Homo sapiens GI:3882321 EMBL:AB018343
Length = 1846
Score = 30.3 bits (65), Expect = 3.6
Identities = 20/71 (28%), Positives = 30/71 (42%), Gaps = 4/71 (5%)
Query: 380 DSRPLQLQTHPNVDKEAFRSTGVIGLKQAQ-RPFPMHSDVGVLKWRLATTNDDKLAPLSV 438
DS+ L L H ++ T LK+AQ P P + + + +T + PL+
Sbjct: 1050 DSKELLLLIHEHLQASGLGDTASALLKEAQLTPLPSSASPSSIAY---STTQEMSTPLAQ 1106
Query: 439 NCWPSEGVNGG 449
WPS N G
Sbjct: 1107 EQWPSGRANSG 1117
>At4g24550.2 68417.m03519 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 451
Score = 30.3 bits (65), Expect = 3.6
Identities = 20/84 (23%), Positives = 42/84 (50%), Gaps = 2/84 (2%)
Query: 116 ESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLF 175
+ V Y + + LY + T + LE L+ +RV+ +Y L E + + F L++
Sbjct: 55 DGVNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNE-DSFRKNFVLVY 113
Query: 176 AF-DEIVALGYRESVNLAQVRSFV 198
DE++ GY ++ + ++S++
Sbjct: 114 ELLDEVIDFGYVQTTSTEVLKSYI 137
>At4g24550.1 68417.m03518 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 380
Score = 30.3 bits (65), Expect = 3.6
Identities = 20/84 (23%), Positives = 42/84 (50%), Gaps = 2/84 (2%)
Query: 116 ESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLF 175
+ V Y + + LY + T + LE L+ +RV+ +Y L E + + F L++
Sbjct: 55 DGVNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNE-DSFRKNFVLVY 113
Query: 176 AF-DEIVALGYRESVNLAQVRSFV 198
DE++ GY ++ + ++S++
Sbjct: 114 ELLDEVIDFGYVQTTSTEVLKSYI 137
>At1g22000.1 68414.m02752 F-box family protein contains F-box domain
Pfam:PF00646
Length = 727
Score = 30.3 bits (65), Expect = 3.6
Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
Query: 499 WSIPLVSKQQKTGSLEFTVTPSIPNDFFPLSVTWTSETSL 538
W +PL+ Q + L+ TP+I NDF LS++W +S+
Sbjct: 344 WLMPLMFLLQNSPKLK---TPTIDNDFECLSLSWNQPSSI 380
>At5g13760.1 68418.m01604 expressed protein similar to unknown
protein (gb AAF63775.1)
Length = 569
Score = 29.9 bits (64), Expect = 4.8
Identities = 13/41 (31%), Positives = 23/41 (56%)
Query: 145 ETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGY 185
+T +F+ + L T+ N+ F LLF F ++VA+G+
Sbjct: 118 QTRSIFTPTPQQTLASLNSTKYTNKFFLLLFIFHKVVAIGF 158
>At2g19790.1 68415.m02312 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 143
Score = 29.5 bits (63), Expect = 6.3
Identities = 20/98 (20%), Positives = 43/98 (43%)
Query: 103 LMTGGRQHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLT 162
L +Q +FVE + + VY+ L+ ++ N L LE + L + ++ +
Sbjct: 41 LARNDQQCSFVEHRNYKIVYRRYASLFFMVGVDDDENELAILEFIHLLVETMDKHFGNVC 100
Query: 163 ETEVLNQAFNLLFAFDEIVALGYRESVNLAQVRSFVEM 200
E +++ F +E+V G + A + S +++
Sbjct: 101 ELDIMFHLEKAHFMLEEMVMNGCIVETSKANILSPIQL 138
>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 29.5 bits (63), Expect = 6.3
Identities = 28/136 (20%), Positives = 60/136 (44%), Gaps = 4/136 (2%)
Query: 77 GKALVSRQFV-EMTKARIEGLLAAFPKLMTGGRQHTFVETES-VRYVYQPLDKLYMLLIT 134
G+ LV R + ++T A+ E + + + V ++ V Y++ +Y+++ +
Sbjct: 15 GRVLVWRDYRGDVTAAQAERFFTKLIETEGDSQSNDPVAYDNGVTYMFVQHSNIYLMIAS 74
Query: 135 TKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAF-DEIVALGYRESVNLAQ 193
+ N L L V Y +L E E L F +++ DE++ GY +
Sbjct: 75 RQNCNAASLLFFLHRVVDVFKHYFEELEE-ESLRDNFVVVYELLDEMMDFGYPQFTEARI 133
Query: 194 VRSFVEMDSHEEKIYQ 209
+ F++ D++ ++ Q
Sbjct: 134 LSEFIKTDAYRMEVTQ 149
>At5g12900.1 68418.m01480 expressed protein
Length = 562
Score = 29.1 bits (62), Expect = 8.3
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Query: 465 LTDVNITIPLPSGNTSVVVHQWEGSYTQKGRNLIWSIPLVSKQQKTGSL 513
L N+ +PL +++VV Q + GR + W P + K+QK+ S+
Sbjct: 179 LKSSNLVVPLTDSHSAVVSSQ---PRNRGGRVMSWLFPKLKKKQKSNSI 224
>At4g35410.1 68417.m05029 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 124
Score = 29.1 bits (62), Expect = 8.3
Identities = 16/65 (24%), Positives = 29/65 (44%)
Query: 103 LMTGGRQHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLT 162
L G + FVE + VY+ LY + + N LE LE + + ++ Y +
Sbjct: 40 LNRGPKLCNFVEWRGYKVVYKRYASLYFCMCIDQEDNELEVLEIIHHYVEILDRYFGSVC 99
Query: 163 ETEVL 167
E +++
Sbjct: 100 ELDLI 104
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.133 0.385
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,324,055
Number of Sequences: 28952
Number of extensions: 501499
Number of successful extensions: 1084
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1073
Number of HSP's gapped (non-prelim): 15
length of query: 571
length of database: 12,070,560
effective HSP length: 85
effective length of query: 486
effective length of database: 9,609,640
effective search space: 4670285040
effective search space used: 4670285040
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 62 (29.1 bits)
- SilkBase 1999-2023 -