Silkworm BLAST Result against tribolium

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000101-TA|BGIBMGA000101-PA|IPR002048|Calcium-binding
EF-hand
         (1694 letters)

Database: tribolium 
           317 sequences; 114,650 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY043293-2|AAK96034.1|  353|Tribolium castaneum homeodomain tran...    28   0.63 
AF230313-1|AAF64147.1|  210|Tribolium castaneum labial protein p...    28   0.63 
AF230312-1|AAF64146.1|  253|Tribolium castaneum labial protein p...    28   0.63 
AY295879-1|AAQ62692.1| 1464|Tribolium castaneum chitin synthase ...    27   1.5  
AY291477-1|AAQ55061.1| 1464|Tribolium castaneum chitin synthase ...    27   1.5  
AY695256-1|AAW21973.1|  168|Tribolium castaneum ventral nerve co...    26   1.9  
AY008296-1|AAG22858.1|  556|Tribolium castaneum Dorsal protein.        26   1.9  
AF231104-1|AAF64149.1|  142|Tribolium castaneum labial protein p...    25   3.3  
AF231103-1|AAF64148.1|  142|Tribolium castaneum labial protein p...    25   3.3  
AY362543-1|AAQ63455.1|  677|Tribolium castaneum chitin synthase ...    25   4.4  
AY321476-2|AAQ23387.1|  257|Tribolium castaneum Ase protein.           25   4.4  
AY043293-1|AAK96033.1|  523|Tribolium castaneum homeodomain tran...    25   5.9  
AF187069-1|AAF03889.1|  471|Tribolium castaneum proboscipedia or...    25   5.9  
AF187068-1|AAF03888.1|  477|Tribolium castaneum proboscipedia or...    25   5.9  

>AY043293-2|AAK96034.1|  353|Tribolium castaneum homeodomain
           transcription factor Labialprotein.
          Length = 353

 Score = 27.9 bits (59), Expect = 0.63
 Identities = 21/71 (29%), Positives = 28/71 (39%), Gaps = 10/71 (14%)

Query: 122 YTNVDNKNVRFPPF--VRTNNPYEQYMEN-----NPASSQARPQHIQSQETIQPMYSGGY 174
           YTN+D  N  FPP      N PY++  EN     +   +     H   +   Q     GY
Sbjct: 85  YTNLDYGNSNFPPINHQNYNEPYQRTHENMLLRHHEEVADGHQHHFLHENKYQLEVDSGY 144

Query: 175 PSSHKGNASQP 185
              H G +S P
Sbjct: 145 ---HLGASSTP 152



 Score = 25.4 bits (53), Expect = 3.3
 Identities = 15/58 (25%), Positives = 31/58 (53%), Gaps = 2/58 (3%)

Query: 1422 NTTVIKVGNKVINIKESTDRREKQGVDDPKPIVSINEVAKTKSNTTKNEVGNKAVKIN 1479
            N T +K+  +   +K+   +R K+G+  P+PI +    + + S ++ N + N+A   N
Sbjct: 293  NETQVKIWFQNRRMKQK--KRMKEGLIPPEPISASLSTSNSNSPSSTNLIQNEASNEN 348


>AF230313-1|AAF64147.1|  210|Tribolium castaneum labial protein
           protein.
          Length = 210

 Score = 27.9 bits (59), Expect = 0.63
 Identities = 21/71 (29%), Positives = 28/71 (39%), Gaps = 10/71 (14%)

Query: 122 YTNVDNKNVRFPPF--VRTNNPYEQYMEN-----NPASSQARPQHIQSQETIQPMYSGGY 174
           YTN+D  N  FPP      N PY++  EN     +   +     H   +   Q     GY
Sbjct: 85  YTNLDYGNSNFPPINHQNYNEPYQRTHENMLLRHHEEVADGHQHHFLHENKYQLEVDSGY 144

Query: 175 PSSHKGNASQP 185
              H G +S P
Sbjct: 145 ---HLGASSTP 152


>AF230312-1|AAF64146.1|  253|Tribolium castaneum labial protein
           protein.
          Length = 253

 Score = 27.9 bits (59), Expect = 0.63
 Identities = 21/71 (29%), Positives = 28/71 (39%), Gaps = 10/71 (14%)

Query: 122 YTNVDNKNVRFPPF--VRTNNPYEQYMEN-----NPASSQARPQHIQSQETIQPMYSGGY 174
           YTN+D  N  FPP      N PY++  EN     +   +     H   +   Q     GY
Sbjct: 85  YTNLDYGNSNFPPINHQNYNEPYQRTHENMLLRHHEEVADGHQHHFLHENKYQLEVDSGY 144

Query: 175 PSSHKGNASQP 185
              H G +S P
Sbjct: 145 ---HLGASSTP 152


>AY295879-1|AAQ62692.1| 1464|Tribolium castaneum chitin synthase
           protein.
          Length = 1464

 Score = 26.6 bits (56), Expect = 1.5
 Identities = 11/37 (29%), Positives = 19/37 (51%)

Query: 137 RTNNPYEQYMENNPASSQARPQHIQSQETIQPMYSGG 173
           R  NP     +  P  ++  P+ I+SQ+ I  +Y+ G
Sbjct: 503 RDENPRNYRSDEGPQITELEPETIESQDAITRIYACG 539


>AY291477-1|AAQ55061.1| 1464|Tribolium castaneum chitin synthase
           CHS2 protein.
          Length = 1464

 Score = 26.6 bits (56), Expect = 1.5
 Identities = 11/37 (29%), Positives = 19/37 (51%)

Query: 137 RTNNPYEQYMENNPASSQARPQHIQSQETIQPMYSGG 173
           R  NP     +  P  ++  P+ I+SQ+ I  +Y+ G
Sbjct: 503 RDENPRNYRSDEGPQITELEPETIESQDAITRIYACG 539


>AY695256-1|AAW21973.1|  168|Tribolium castaneum ventral nerve cord
            defective protein protein.
          Length = 168

 Score = 26.2 bits (55), Expect = 1.9
 Identities = 13/34 (38%), Positives = 22/34 (64%), Gaps = 1/34 (2%)

Query: 1172 QDYRLYRSNRRESEKKLKENQRNDVRDNIRRVAV 1205
            Q++R Y++ R + EK + + Q N+   + RRVAV
Sbjct: 89   QNHR-YKTKRAQHEKGMHDQQNNNPLPSPRRVAV 121


>AY008296-1|AAG22858.1|  556|Tribolium castaneum Dorsal protein.
          Length = 556

 Score = 26.2 bits (55), Expect = 1.9
 Identities = 13/44 (29%), Positives = 17/44 (38%)

Query: 639 HNVVPAPCEKPYHQNQVPDYNSSLGGTAPSLGFNLYLCQENFEP 682
           H V P P E P  QN +P     +    PS      +   N+ P
Sbjct: 416 HYVTPTPPEAPLFQNVLPPIGGMMNWNMPSTSVVGDMLNMNWNP 459


>AF231104-1|AAF64149.1|  142|Tribolium castaneum labial protein
            protein.
          Length = 142

 Score = 25.4 bits (53), Expect = 3.3
 Identities = 15/58 (25%), Positives = 31/58 (53%), Gaps = 2/58 (3%)

Query: 1422 NTTVIKVGNKVINIKESTDRREKQGVDDPKPIVSINEVAKTKSNTTKNEVGNKAVKIN 1479
            N T +K+  +   +K+   +R K+G+  P+PI +    + + S ++ N + N+A   N
Sbjct: 82   NETQVKIWFQNRRMKQK--KRMKEGLIPPEPISASLSTSNSNSPSSTNLIQNEASNEN 137


>AF231103-1|AAF64148.1|  142|Tribolium castaneum labial protein
            protein.
          Length = 142

 Score = 25.4 bits (53), Expect = 3.3
 Identities = 15/58 (25%), Positives = 31/58 (53%), Gaps = 2/58 (3%)

Query: 1422 NTTVIKVGNKVINIKESTDRREKQGVDDPKPIVSINEVAKTKSNTTKNEVGNKAVKIN 1479
            N T +K+  +   +K+   +R K+G+  P+PI +    + + S ++ N + N+A   N
Sbjct: 82   NETQVKIWFQNRRMKQK--KRMKEGLIPPEPISASLSTSNSNSPSSTNLIQNEASNEN 137


>AY362543-1|AAQ63455.1|  677|Tribolium castaneum chitin synthase
           protein.
          Length = 677

 Score = 25.0 bits (52), Expect = 4.4
 Identities = 10/35 (28%), Positives = 18/35 (51%)

Query: 137 RTNNPYEQYMENNPASSQARPQHIQSQETIQPMYS 171
           R  NP     +  P  ++  P+ I+SQ+ I  +Y+
Sbjct: 270 RDENPRTYRSDEGPQITELEPETIESQDAITRIYA 304


>AY321476-2|AAQ23387.1|  257|Tribolium castaneum Ase protein.
          Length = 257

 Score = 25.0 bits (52), Expect = 4.4
 Identities = 15/42 (35%), Positives = 19/42 (45%)

Query: 1383 KTRKTETLTRDTKSAEAKHDITALSNSTVSKLDVSKSKSNTT 1424
            K  K ETL    +   +  D+ AL  STVS    S    +TT
Sbjct: 97   KLSKVETLRMAVEYIRSLEDLLALDESTVSSSTSSIPSPSTT 138


>AY043293-1|AAK96033.1|  523|Tribolium castaneum homeodomain
            transcription factor Maxillopediaprotein.
          Length = 523

 Score = 24.6 bits (51), Expect = 5.9
 Identities = 15/58 (25%), Positives = 28/58 (48%)

Query: 1383 KTRKTETLTRDTKSAEAKHDITALSNSTVSKLDVSKSKSNTTVIKVGNKVINIKESTD 1440
            K  + ++ + +  S      IT  +NS   K++  ++  N+   K+G   I+ KES D
Sbjct: 159  KLIEEDSRSNEDTSLHPSPRITKKANSVSVKVENRRNSPNSCEKKIGLSKISPKESQD 216


>AF187069-1|AAF03889.1|  471|Tribolium castaneum proboscipedia
            ortholog protein.
          Length = 471

 Score = 24.6 bits (51), Expect = 5.9
 Identities = 15/58 (25%), Positives = 28/58 (48%)

Query: 1383 KTRKTETLTRDTKSAEAKHDITALSNSTVSKLDVSKSKSNTTVIKVGNKVINIKESTD 1440
            K  + ++ + +  S      IT  +NS   K++  ++  N+   K+G   I+ KES D
Sbjct: 107  KLIEEDSRSNEDTSLHPSPRITKKANSVSVKVENRRNSPNSCEKKIGLSKISPKESQD 164


>AF187068-1|AAF03888.1|  477|Tribolium castaneum proboscipedia
            ortholog protein.
          Length = 477

 Score = 24.6 bits (51), Expect = 5.9
 Identities = 15/58 (25%), Positives = 28/58 (48%)

Query: 1383 KTRKTETLTRDTKSAEAKHDITALSNSTVSKLDVSKSKSNTTVIKVGNKVINIKESTD 1440
            K  + ++ + +  S      IT  +NS   K++  ++  N+   K+G   I+ KES D
Sbjct: 290  KLIEEDSRSNEDTSLHPSPRITKKANSVSVKVENRRNSPNSCEKKIGLSKISPKESQD 347


  Database: tribolium
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 114,650
  Number of sequences in database:  317
  
Lambda     K      H
   0.314    0.131    0.378 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 371,871
Number of Sequences: 317
Number of extensions: 16496
Number of successful extensions: 53
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 38
Number of HSP's gapped (non-prelim): 19
length of query: 1694
length of database: 114,650
effective HSP length: 66
effective length of query: 1628
effective length of database: 93,728
effective search space: 152589184
effective search space used: 152589184
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 50 (24.2 bits)