BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000092-TA|BGIBMGA000092-PA|IPR007243|Autophagy protein Apg6, IPR013819|Lipoxygenase, C-terminal (427 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g61710.1 68416.m06915 autophagy protein Apg6 family contains ... 165 7e-41 At3g61710.2 68416.m06916 autophagy protein Apg6 family contains ... 103 3e-22 At4g25540.1 68417.m03682 DNA mismatch repair protein MSH3 (MSH3)... 30 2.6 At5g65210.2 68418.m08204 bZIP family transcription factor (TGA1)... 30 3.4 At5g65210.1 68418.m08203 bZIP family transcription factor (TGA1)... 30 3.4 >At3g61710.1 68416.m06915 autophagy protein Apg6 family contains weak similarity to Beclin 1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197) (Swiss-Prot:Q14457) [Homo sapiens]; contains Pfam profile PF04111: Autophagy protein Apg6 Length = 517 Score = 165 bits (400), Expect = 7e-41 Identities = 104/342 (30%), Positives = 156/342 (45%), Gaps = 20/342 (5%) Query: 83 TTSLGHQLHVKATLFDLLSNNSYVDHPLCDECTDTLLELMDNQLKQTEAEWXXXXXXXXX 142 T+ ++V FD+ + V+ PLC EC L + ++ +++ + Sbjct: 144 TSGFNATINVLTRAFDIARTQTQVEQPLCLECMRVLSDKLEKEVEDVTRDVEAYEACVQR 203 Query: 143 XXXXXXXXXXXXXXXXXXXWKQEQSRXXXXXXXXXXXXRA-MNXXXXXXXXXXXXLDKEQ 201 +E+ R A +N ++ + Sbjct: 204 LEGETQDVLSEADFLKEKKKIEEEERKLVAAIEETEKQNAEVNHQLKELEFKGNRFNELE 263 Query: 202 DVYWREYTKYRKDLMTTEDQMKFYECQLAYTQSQLEKLKKTNVFKATFHISDSGQFGIIN 261 D YW+E+ ++ L+ +++ ++ +Q+ LE L KTNV F I + G+FG IN Sbjct: 264 DRYWQEFNNFQFQLIAHQEERDAILAKIEVSQAHLELLNKTNVLIDAFPIRNDGEFGTIN 323 Query: 262 NFRLGRLPTAPVDWSEINAAWGQTVLLLSSLARKINFNFQ-RYKLVPYGNHSYIEVLEDQ 320 NFRLGRLP V+W EINAAWGQ LLL ++ FQ + K+ P G++ I V + Sbjct: 324 NFRLGRLPAIKVEWDEINAAWGQACLLLHTMCNYFRPKFQCQVKIQPMGSYPRI-VDSNN 382 Query: 321 KVLPLYGSGGFRFLWDTKFDAAMVAFLDCLQQF------KEQVEMGNTGFC--LPYRIDK 372 + L+G W T++D AM +L CL+ F K+Q C LPY+I+K Sbjct: 383 ETYELFGP--VNLFWSTRYDKAMTLYLMCLKDFADFANSKDQENNIPPDNCLNLPYKIEK 440 Query: 373 GKIEDTASPPHAYSIKIQFNSEEHWTKALKYMLTNLKWALTW 414 K+ YSI FN +E WTKALKY L NLKWAL W Sbjct: 441 DKV-------LGYSITQSFNKQESWTKALKYTLCNLKWALYW 475 >At3g61710.2 68416.m06916 autophagy protein Apg6 family contains weak similarity to Beclin 1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197) (Swiss-Prot:Q14457) [Homo sapiens]; contains Pfam profile PF04111: Autophagy protein Apg6 Length = 386 Score = 103 bits (246), Expect = 3e-22 Identities = 58/220 (26%), Positives = 94/220 (42%), Gaps = 1/220 (0%) Query: 83 TTSLGHQLHVKATLFDLLSNNSYVDHPLCDECTDTLLELMDNQLKQTEAEWXXXXXXXXX 142 T+ ++V FD+ + V+ PLC EC L + ++ +++ + Sbjct: 144 TSGFNATINVLTRAFDIARTQTQVEQPLCLECMRVLSDKLEKEVEDVTRDVEAYEACVQR 203 Query: 143 XXXXXXXXXXXXXXXXXXXWKQEQSRXXXXXXXXXXXXRA-MNXXXXXXXXXXXXLDKEQ 201 +E+ R A +N ++ + Sbjct: 204 LEGETQDVLSEADFLKEKKKIEEEERKLVAAIEETEKQNAEVNHQLKELEFKGNRFNELE 263 Query: 202 DVYWREYTKYRKDLMTTEDQMKFYECQLAYTQSQLEKLKKTNVFKATFHISDSGQFGIIN 261 D YW+E+ ++ L+ +++ ++ +Q+ LE L KTNV F I + G+FG IN Sbjct: 264 DRYWQEFNNFQFQLIAHQEERDAILAKIEVSQAHLELLNKTNVLIDAFPIRNDGEFGTIN 323 Query: 262 NFRLGRLPTAPVDWSEINAAWGQTVLLLSSLARKINFNFQ 301 NFRLGRLP V+W EINAAWGQ LLL ++ FQ Sbjct: 324 NFRLGRLPAIKVEWDEINAAWGQACLLLHTMCNYFRPKFQ 363 >At4g25540.1 68417.m03682 DNA mismatch repair protein MSH3 (MSH3) identical to SP|O65607 DNA mismatch repair protein MSH3 (AtMsh3) {Arabidopsis thaliana} Length = 1081 Score = 30.3 bits (65), Expect = 2.6 Identities = 14/35 (40%), Positives = 20/35 (57%) Query: 78 SDGWETTSLGHQLHVKATLFDLLSNNSYVDHPLCD 112 SDG E+ SL H ++ T++ +V HPLCD Sbjct: 437 SDGSESGSLFHNMNHTLTVYGSRLLRHWVTHPLCD 471 >At5g65210.2 68418.m08204 bZIP family transcription factor (TGA1) identical to transcription factor (TGA1) GI:16550 from [Arabidopsis thaliana] Length = 368 Score = 29.9 bits (64), Expect = 3.4 Identities = 15/42 (35%), Positives = 25/42 (59%), Gaps = 3/42 (7%) Query: 47 DLDLQS---SSLDHYVPPFRMSESGNGANGFMVISDGWETTS 85 D++L+S +++ HY FRM S A+ F V+S W T++ Sbjct: 184 DIELRSLVENAMKHYFELFRMKSSAAKADVFFVMSGMWRTSA 225 >At5g65210.1 68418.m08203 bZIP family transcription factor (TGA1) identical to transcription factor (TGA1) GI:16550 from [Arabidopsis thaliana] Length = 368 Score = 29.9 bits (64), Expect = 3.4 Identities = 15/42 (35%), Positives = 25/42 (59%), Gaps = 3/42 (7%) Query: 47 DLDLQS---SSLDHYVPPFRMSESGNGANGFMVISDGWETTS 85 D++L+S +++ HY FRM S A+ F V+S W T++ Sbjct: 184 DIELRSLVENAMKHYFELFRMKSSAAKADVFFVMSGMWRTSA 225 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.319 0.133 0.411 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,195,692 Number of Sequences: 28952 Number of extensions: 357755 Number of successful extensions: 798 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 789 Number of HSP's gapped (non-prelim): 7 length of query: 427 length of database: 12,070,560 effective HSP length: 83 effective length of query: 344 effective length of database: 9,667,544 effective search space: 3325635136 effective search space used: 3325635136 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 61 (28.7 bits)
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