BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000086-TA|BGIBMGA000086-PA|IPR002589|Appr-1-p processing (244 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g69340.1 68414.m07956 appr-1-p processing enzyme family prote... 144 4e-35 At2g40600.1 68415.m05008 appr-1-p processing enzyme family prote... 133 1e-31 At5g01310.1 68418.m00043 basic helix-loop-helix (bHLH) family pr... 41 7e-04 At1g79280.1 68414.m09242 expressed protein weak similarity to Nu... 37 0.014 At4g17520.1 68417.m02621 nuclear RNA-binding protein, putative s... 34 0.10 At4g32630.1 68417.m04645 hypothetical protein 32 0.41 At2g38090.1 68415.m04676 myb family transcription factor contain... 32 0.41 At3g03800.1 68416.m00390 syntaxin, putative (SYP131) similar to ... 31 0.71 At5g05210.1 68418.m00555 nucleolar matrix protein-related contai... 30 1.2 At4g29090.1 68417.m04163 reverse transcriptase, putative / RNA-d... 30 1.2 At5g52280.1 68418.m06488 protein transport protein-related low s... 30 1.6 At4g39520.1 68417.m05588 GTP-binding protein, putative similar t... 29 2.2 At3g28770.1 68416.m03591 expressed protein 29 2.9 At2g33870.1 68415.m04158 Ras-related GTP-binding protein, putati... 29 2.9 At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family pr... 29 3.8 At3g09040.1 68416.m01063 pentatricopeptide (PPR) repeat-containi... 29 3.8 At5g24880.1 68418.m02946 expressed protein ; expression supporte... 28 5.0 At1g28550.1 68414.m03513 Ras-related GTP-binding protein, putati... 28 5.0 At3g18290.1 68416.m02326 zinc finger protein-related weak alignm... 28 6.6 At1g65070.1 68414.m07377 DNA mismatch repair MutS family protein... 28 6.6 At3g54760.1 68416.m06059 dentin sialophosphoprotein-related cont... 27 8.8 At2g17370.1 68415.m02006 3-hydroxy-3-methylglutaryl-CoA reductas... 27 8.8 At1g64610.2 68414.m07324 WD-40 repeat family protein contains Pf... 27 8.8 At1g64610.1 68414.m07323 WD-40 repeat family protein contains Pf... 27 8.8 >At1g69340.1 68414.m07956 appr-1-p processing enzyme family protein contains Pfam domain PF01661: Appr-1-p processing enzyme family Length = 562 Score = 144 bits (350), Expect = 4e-35 Identities = 73/174 (41%), Positives = 104/174 (59%), Gaps = 8/174 (4%) Query: 76 ISERVSIFKGDITKLEIDAVVNAANSRLKAGGGVDGAIHRAAGPFLQAECDSIGGCPTGD 135 I+ R+ +++G+ LE+DAVVN+ N L G +H AAGP L +C ++GGC TG Sbjct: 83 INSRIYLWRGEPWNLEVDAVVNSTNENLDEAHSSPG-LHVAAGPGLAEQCATLGGCRTGM 141 Query: 136 AKVTGGYNLPAKYIIHTVGPQ------DGSAEKLESCYEKCLSFQQEYQIKSIAFPCIST 189 AKVT Y+LPA+ +IHTVGP+ + L CY CL + ++SIA CI T Sbjct: 142 AKVTNAYDLPARRVIHTVGPKYAVKYHTAAENALSHCYRSCLELLIDSGLQSIALGCIYT 201 Query: 190 GIYGFPNRLAAHIALRTARKFLETNTE-MNRIIFCTFLPIDVEIYETLMQLYFP 242 +P AAH+A+RT R+FLE + ++ ++FCT D EIY+ L+ LYFP Sbjct: 202 EAKNYPREPAAHVAIRTVRRFLEKQKDKISAVVFCTTTSSDTEIYKRLLPLYFP 255 >At2g40600.1 68415.m05008 appr-1-p processing enzyme family protein contains Pfam domain PF01661: Appr-1-p processing enzyme family Length = 257 Score = 133 bits (321), Expect = 1e-31 Identities = 76/164 (46%), Positives = 98/164 (59%), Gaps = 14/164 (8%) Query: 73 NKSISERVSIFKGDITKLEID----AVVNAANSRLKAGGGVDGAIHRAAGPFLQAECDSI 128 N S S + I KGDITK +D A+VN AN R+ GGG DGAIHRAAGP L+A C + Sbjct: 75 NLSDSSLLKILKGDITKWSVDSSSDAIVNPANERMLGGGGADGAIHRAAGPQLRAACYEV 134 Query: 129 G------GCPTGDAKVTGGYNLPAKYIIHTVGPQDGS----AEKLESCYEKCLSFQQEYQ 178 CPTG+A++T G+NLPA +IHTVGP S E L + Y+ L +E Sbjct: 135 PEVRPGVRCPTGEARITPGFNLPASRVIHTVGPIYDSDVNPQESLTNSYKNSLRVAKENN 194 Query: 179 IKSIAFPCISTGIYGFPNRLAAHIALRTARKFLETNTEMNRIIF 222 IK IAFP IS GIYG+P AA I + T ++F E++ ++F Sbjct: 195 IKYIAFPAISCGIYGYPFDEAAAIGISTIKQFSTDFKEVHFVLF 238 >At5g01310.1 68418.m00043 basic helix-loop-helix (bHLH) family protein contains Pfam profile: PF00010 helix-loop-helix DNA-binding domain, weak hit to PF01661: Appr-1-p processing enzyme family Length = 912 Score = 41.1 bits (92), Expect = 7e-04 Identities = 26/64 (40%), Positives = 34/64 (53%), Gaps = 7/64 (10%) Query: 66 IKINTEKNKSISERVSIFKGDITKLEIDA------VVNAANSRLK-AGGGVDGAIHRAAG 118 +K + S + F GDITKL + + NA N RLK GGGV+ AI +AAG Sbjct: 510 VKAKASQKNIDSAKFFTFVGDITKLRSEGGLHCNVIANATNWRLKPGGGGVNAAIFKAAG 569 Query: 119 PFLQ 122 P L+ Sbjct: 570 PDLE 573 >At1g79280.1 68414.m09242 expressed protein weak similarity to Nucleoprotein TPR (Swiss-Prot:P12270) [Homo sapiens] Length = 2111 Score = 36.7 bits (81), Expect = 0.014 Identities = 25/75 (33%), Positives = 40/75 (53%), Gaps = 3/75 (4%) Query: 3 NSTKWEIEKNRILKLSLE-EKRKIYKSSDFIDLENVDPWSKYLNKSQGIDSKKSTTDDLK 61 N K E+EKN+ + +L KRK K D + +N +K L +++ K++TTD + Sbjct: 1448 NKQKQELEKNKKIHYTLNMTKRKYEKEKDELSKQN-QSLAKQLEEAKEEAGKRTTTDAVV 1506 Query: 62 EFEKIKINTEKNKSI 76 E + +K EK K I Sbjct: 1507 E-QSVKEREEKEKRI 1520 >At4g17520.1 68417.m02621 nuclear RNA-binding protein, putative similar to nuclear RNA binding protein GI:6492264 from [Arabidopsis thaliana] Length = 360 Score = 33.9 bits (74), Expect = 0.10 Identities = 23/61 (37%), Positives = 36/61 (59%), Gaps = 7/61 (11%) Query: 19 LEEKRKIYKSSDFIDLENVDPWSKYLNKSQGIDSKKSTTDDLKEFEKIKINTEKNKSISE 78 LEEK+K +++ ++ VD +K Q + SKKS D++ IK+ TEK+K I+E Sbjct: 225 LEEKKKALQATK-VEERKVD--TKAFEAMQQLSSKKSNNDEVF----IKLGTEKDKRITE 277 Query: 79 R 79 R Sbjct: 278 R 278 >At4g32630.1 68417.m04645 hypothetical protein Length = 531 Score = 31.9 bits (69), Expect = 0.41 Identities = 22/90 (24%), Positives = 44/90 (48%), Gaps = 6/90 (6%) Query: 1 MVNSTKW-EIEKNRILKLSLEEKRKIYKSSDFIDLENVDPWSKYLNKSQGIDSKKSTTDD 59 ++N +W ++ +++ + EE I+K DFI VD +Y + + K T+D Sbjct: 66 VLNLYQWPSSQQTKLVHFAQEETSNIFKLRDFIRSVYVD--KRYSSSDKISQQKSDVTED 123 Query: 60 LKEFEKIKINTEKNKSISERVSIFKGDITK 89 +E +K + ++S+ S+ K DI + Sbjct: 124 YRESKKTSAHVLGSRSLH---SVDKSDIER 150 >At2g38090.1 68415.m04676 myb family transcription factor contains Pfam profile: PF00249 myb-like DNA-binding domain Length = 298 Score = 31.9 bits (69), Expect = 0.41 Identities = 16/66 (24%), Positives = 33/66 (50%), Gaps = 4/66 (6%) Query: 2 VNSTKWEIEKNRILKLSLEEKRKIYKSSDFIDLENVDPWSKYLNKSQGIDSKKSTTDDLK 61 + ++ W ++NR K + EE +K + F D + D WS+ G K+ D +K Sbjct: 14 LETSNWLFQENRGTKWTAEENKKFENALAFYDKDTPDRWSRVAAMLPG----KTVGDVIK 69 Query: 62 EFEKIK 67 ++ +++ Sbjct: 70 QYRELE 75 >At3g03800.1 68416.m00390 syntaxin, putative (SYP131) similar to SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related protein At-Syr1) {Arabidopsis thaliana} Length = 306 Score = 31.1 bits (67), Expect = 0.71 Identities = 21/70 (30%), Positives = 31/70 (44%), Gaps = 1/70 (1%) Query: 42 KYLNKSQGIDSKKSTTDDLKEFEKIKINTEKNKSISERVSIF-KGDITKLEIDAVVNAAN 100 K+LNK QG + + IK E++ R+S F KG I +L+ + + N Sbjct: 54 KHLNKLQGAHEETKAVTKAPAMKSIKQRMERDVDEVGRISRFIKGKIEELDRENLENRTK 113 Query: 101 SRLKAGGGVD 110 G GVD Sbjct: 114 PGCGKGTGVD 123 >At5g05210.1 68418.m00555 nucleolar matrix protein-related contains Pfam domain, PF04935: Surfeit locus protein 6 Length = 386 Score = 30.3 bits (65), Expect = 1.2 Identities = 13/34 (38%), Positives = 23/34 (67%) Query: 40 WSKYLNKSQGIDSKKSTTDDLKEFEKIKINTEKN 73 W L+K+Q +KK T D+LK+ ++ K++ EK+ Sbjct: 52 WYPGLSKAQKARAKKKTNDNLKKAKRDKLDPEKS 85 >At4g29090.1 68417.m04163 reverse transcriptase, putative / RNA-dependent DNA polymerase, putative similar to reverse transcriptase [Arabidopsis thaliana] GI:976278; contains Pfam profile PF00075: RNase H Length = 575 Score = 30.3 bits (65), Expect = 1.2 Identities = 23/95 (24%), Positives = 40/95 (42%), Gaps = 2/95 (2%) Query: 133 TGDAKVTGGYNLPAKYIIHTVGPQDGSAEKLESCYEKCLSFQQEYQIKSIAFPCISTGIY 192 +GD V GY + + I PQ+ S L Y+K Q +I+ + C+S + Sbjct: 220 SGDYTVKSGYWVLTQIINKRSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLP 279 Query: 193 GFPNRLAAHIALRTA-RKFLETNTEMNRIIF-CTF 225 H++ +A + +N ++F CTF Sbjct: 280 VAGALAYRHLSKESACIRCPSCKETVNHLLFKCTF 314 >At5g52280.1 68418.m06488 protein transport protein-related low similarity to SP|P25386 Intracellular protein transport protein USO1 {Saccharomyces cerevisiae} Length = 853 Score = 29.9 bits (64), Expect = 1.6 Identities = 22/81 (27%), Positives = 42/81 (51%), Gaps = 1/81 (1%) Query: 10 EKNRILKLSLEEKRKIYKSSDFIDLENVDPWSKYLNKSQGIDSKKSTTDDLKEFEKIKIN 69 EK + L L +E K +++ L + + NK+ +K+ T+ +E E+ K Sbjct: 585 EKCKRLSLEMESKLSEHENLTKKTLAEANNL-RLQNKTLEEMQEKTHTEITQEKEQRKHV 643 Query: 70 TEKNKSISERVSIFKGDITKL 90 EKNK++S +V + + ++ KL Sbjct: 644 EEKNKALSMKVQMLESEVLKL 664 >At4g39520.1 68417.m05588 GTP-binding protein, putative similar to SP|Q9Y295 Developmentally regulated GTP-binding protein 1 (DRG 1) {Homo sapiens}; contains Pfam profiles PF02824: TGS domain, PF01018: GTP1/OBG family Length = 369 Score = 29.5 bits (63), Expect = 2.2 Identities = 17/52 (32%), Positives = 24/52 (46%), Gaps = 4/52 (7%) Query: 60 LKEFEKIKINTEKNKSISERVSIFKGDITKLEIDAVVNAANSRLKAGGGVDG 111 +KE E T+KNK+ S + + K + KL D + K GGG G Sbjct: 8 IKEIEDEMAKTQKNKATSHHLGLLKAKLAKLRRDLLAPPT----KGGGGGAG 55 >At3g28770.1 68416.m03591 expressed protein Length = 2081 Score = 29.1 bits (62), Expect = 2.9 Identities = 22/84 (26%), Positives = 36/84 (42%) Query: 10 EKNRILKLSLEEKRKIYKSSDFIDLENVDPWSKYLNKSQGIDSKKSTTDDLKEFEKIKIN 69 E+++ K EE + K D D+E ++ + K + KKS L + E K Sbjct: 1096 EEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNKKKEDKNEKKKSQHVKLVKKESDKKE 1155 Query: 70 TEKNKSISERVSIFKGDITKLEID 93 ++N+ SE I K E+D Sbjct: 1156 KKENEEKSETKEIESSKSQKNEVD 1179 >At2g33870.1 68415.m04158 Ras-related GTP-binding protein, putative similar to GTP-binding protein GI:303742 from [Pisum sativum] Length = 219 Score = 29.1 bits (62), Expect = 2.9 Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 1/75 (1%) Query: 25 IYKSSDFIDLENVDPWSKYLNKSQGIDSKKSTTDDLKEFEKIK-INTEKNKSISERVSIF 83 +Y + + ENV+ W K L ++ + + ++ I+TE+ K +ER + F Sbjct: 93 VYDVTRHVTFENVERWLKELRDHTDANTVIMLVGNKADLNHLRAISTEEVKDFAERENTF 152 Query: 84 KGDITKLEIDAVVNA 98 + + LE V NA Sbjct: 153 FMETSALEAINVENA 167 >At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family protein similar to 3-hydroxybutyryl-CoA dehydratase (Crotonase) from Clostridium acetobutylicum [SP|P52046], FadB1x (enoyl-CoA hydratase) from Pseudomonas putida [GI:13310130]; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein Length = 265 Score = 28.7 bits (61), Expect = 3.8 Identities = 27/90 (30%), Positives = 38/90 (42%), Gaps = 8/90 (8%) Query: 44 LNKSQGIDSKKSTTD-DLKEFEKIKINTEKNKSISERV------SIFKGDITKLEIDAVV 96 L ++ +D K+ D D E ++ I T +S V S+FKGD+ E D VV Sbjct: 33 LTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRSFCSGVDLTAAESVFKGDVKDPETDPVV 92 Query: 97 NAANSRLKAGGGVDGAIHRAAGPFLQAECD 126 R G ++G AG L CD Sbjct: 93 QMERLRKPIIGAING-FAITAGFELALACD 121 >At3g09040.1 68416.m01063 pentatricopeptide (PPR) repeat-containing protein contains INTERPRO:IPR002885 PPR repeats Length = 1028 Score = 28.7 bits (61), Expect = 3.8 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 2/38 (5%) Query: 194 FPNRLAAHIALRTARKFLETNTEMNRIIFCTFLPIDVE 231 FPN+ I L T + ETN E R I C+ + + +E Sbjct: 157 FPNKFTFSIVLSTCAR--ETNVEFGRQIHCSMIKMGLE 192 >At5g24880.1 68418.m02946 expressed protein ; expression supported by MPSS Length = 443 Score = 28.3 bits (60), Expect = 5.0 Identities = 19/84 (22%), Positives = 41/84 (48%), Gaps = 1/84 (1%) Query: 10 EKNRILKLSLEEKRKIYK-SSDFIDLENVDPWSKYLNKSQGIDSKKSTTDDLKEFEKIKI 68 E+ +K ++E K ++ ++E+V+ ++ + + K+ ++ KE EK+K Sbjct: 300 EEEEDVKKKIDENETPEKVDTESKEVESVEETTQEKEEEVKEEGKERVEEEEKEKEKVKE 359 Query: 69 NTEKNKSISERVSIFKGDITKLEI 92 + +K K E KGD K ++ Sbjct: 360 DDQKEKVEEEEKEKVKGDEEKEKV 383 >At1g28550.1 68414.m03513 Ras-related GTP-binding protein, putative similar to GTP-binding protein GI:303742 from (Pisum sativum) Length = 218 Score = 28.3 bits (60), Expect = 5.0 Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 1/75 (1%) Query: 25 IYKSSDFIDLENVDPWSKYLNKSQGIDSKKSTTDDLKEFEKIK-INTEKNKSISERVSIF 83 +Y + + ENV+ W K L + + + ++ I+TE+ K+ +ER + F Sbjct: 92 VYDVTRHVTFENVERWLKELRDHTDANIVIMLVGNKADLRHLRAISTEEAKAFAERENTF 151 Query: 84 KGDITKLEIDAVVNA 98 + + LE V NA Sbjct: 152 FMETSALEAVNVDNA 166 >At3g18290.1 68416.m02326 zinc finger protein-related weak alignment to Pfam profiles: PF00097 Zinc finger, C3HC4 type (RING finger) (2 copies) Length = 1254 Score = 27.9 bits (59), Expect = 6.6 Identities = 14/38 (36%), Positives = 19/38 (50%) Query: 7 WEIEKNRILKLSLEEKRKIYKSSDFIDLENVDPWSKYL 44 W N+ +K +E RKI S DF DL D +Y+ Sbjct: 321 WHKSINKEMKEIADEARKIQLSGDFSDLSAFDERLQYI 358 >At1g65070.1 68414.m07377 DNA mismatch repair MutS family protein contains Pfam profile PF00488: MutS domain V Length = 857 Score = 27.9 bits (59), Expect = 6.6 Identities = 14/40 (35%), Positives = 21/40 (52%), Gaps = 1/40 (2%) Query: 34 LENVDPWSKYLNKSQGIDSKKSTTDDL-KEFEKIKINTEK 72 LEN W++ LN Q ++ K S L +E K+K+ K Sbjct: 572 LENAHKWTEKLNPEQDVERKGSLFQSLMEERNKLKLQATK 611 >At3g54760.1 68416.m06059 dentin sialophosphoprotein-related contains weak similarity to Swiss-Prot:Q9NZW4 dentin sialophosphoprotein precursor (Dentin phosphophoryn DPP, Dentin sialoprotein DSP) [Homo sapiens] Length = 792 Score = 27.5 bits (58), Expect = 8.8 Identities = 20/89 (22%), Positives = 42/89 (47%), Gaps = 4/89 (4%) Query: 17 LSLEEKRKIYKSSDFIDLENVDPWSKYLNKSQGIDSKKSTTDDLKEFEKIKINTEKNKSI 76 +SL+E +K+ ++ + +++ S G +S + TD KE + +E+N+ Sbjct: 365 VSLKEDKKVTDMAEDVVTADIETESNEARVGVGAESPAAATDCSKETSDATLGSEENQQD 424 Query: 77 SERVSIFK--GDI--TKLEIDAVVNAANS 101 + + K D+ T +E D + + A S Sbjct: 425 KDHQCLDKKTADVQDTMIEEDDITHEAPS 453 >At2g17370.1 68415.m02006 3-hydroxy-3-methylglutaryl-CoA reductase 2 / HMG-CoA reductase 2 (HMGR2) identical to SP|P43256 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 (EC 1.1.1.34) (HMG- CoA reductase 2) (HMGR2) {Arabidopsis thaliana} Length = 562 Score = 27.5 bits (58), Expect = 8.8 Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 5/68 (7%) Query: 140 GGYNLPAKYIIHTVGPQDGS--AEKLES--CYEKCLSFQQEYQIKSIAFPCISTGIYGFP 195 GG+N + I+ V G A+ +ES C L + I S++ PCI G G Sbjct: 428 GGFNAHSSNIVSAVFIATGQDPAQNVESSHCMTMILPDGDDLHI-SVSMPCIEVGTVGGG 486 Query: 196 NRLAAHIA 203 +LA+ A Sbjct: 487 TQLASQAA 494 >At1g64610.2 68414.m07324 WD-40 repeat family protein contains Pfam PF00400: WD domain, G-beta repeat; similar to WD-repeat protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo sapiens] Length = 647 Score = 27.5 bits (58), Expect = 8.8 Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 1/51 (1%) Query: 35 ENVDPWSKYLNKSQGIDSKKSTTDDLKEFEKIKINTEKNKSISERVSIFKG 85 E+V + +S G+ KK+ TDD E E +T+ N +SE V++ G Sbjct: 34 ESVSTRRRKFLQSMGLSFKKTATDDDSEDELEPSHTDSN-VVSEVVNVSSG 83 >At1g64610.1 68414.m07323 WD-40 repeat family protein contains Pfam PF00400: WD domain, G-beta repeat; similar to WD-repeat protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo sapiens] Length = 647 Score = 27.5 bits (58), Expect = 8.8 Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 1/51 (1%) Query: 35 ENVDPWSKYLNKSQGIDSKKSTTDDLKEFEKIKINTEKNKSISERVSIFKG 85 E+V + +S G+ KK+ TDD E E +T+ N +SE V++ G Sbjct: 34 ESVSTRRRKFLQSMGLSFKKTATDDDSEDELEPSHTDSN-VVSEVVNVSSG 83 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 5,778,316 Number of Sequences: 28952 Number of extensions: 241677 Number of successful extensions: 738 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 716 Number of HSP's gapped (non-prelim): 30 length of query: 244 length of database: 12,070,560 effective HSP length: 79 effective length of query: 165 effective length of database: 9,783,352 effective search space: 1614253080 effective search space used: 1614253080 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 58 (27.5 bits)
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