BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000078-TA|BGIBMGA000078-PA|IPR012496|TMC (612 letters) Database: mosquito 2123 sequences; 516,269 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AY263177-1|AAP78792.1| 699|Anopheles gambiae TmcC-like protein ... 128 3e-31 AY263175-1|AAP78790.1| 814|Anopheles gambiae TmcA-like protein ... 69 3e-13 AY263176-1|AAP78791.1| 705|Anopheles gambiae TmcB-like protein ... 50 1e-07 M93690-1|AAA29364.1| 613|Anopheles gambiae ORF1 protein. 26 2.5 AY391746-1|AAR28996.1| 502|Anopheles gambiae putative GPCR prot... 25 4.4 AY391745-1|AAR28995.1| 460|Anopheles gambiae putative GPCR prot... 25 4.4 AY056833-1|AAL23627.1| 1253|Anopheles gambiae chitin synthase pr... 25 4.4 AF533893-1|AAM97678.1| 570|Anopheles gambiae ascorbate transpor... 25 5.9 >AY263177-1|AAP78792.1| 699|Anopheles gambiae TmcC-like protein protein. Length = 699 Score = 128 bits (310), Expect = 3e-31 Identities = 56/134 (41%), Positives = 85/134 (63%) Query: 283 TKLFYNMPMAYILVPISWTLLSLIAIVKTAARGFKQKLIESEGQFYKYCNLVFGGWDFCI 342 T L+Y++P+ Y+++ + +++L+AI++ R FK ++ E EG FY+YCNLVFGGWDFCI Sbjct: 44 TILYYDLPLVYVIIIAIYYIITLVAIMRAVVRQFKDRIAEGEGLFYQYCNLVFGGWDFCI 103 Query: 343 HNDRSARIKHQALYNEIKGCIEEERIKEEKQSRSRESQILMHLKRXXXXXXXXXXXXASG 402 HN +SA IKH+AL+NEIK + ++R + E+ +RSRE + + R + Sbjct: 104 HNQKSADIKHRALHNEIKSLLYQKRFEHERNNRSREFMLKLIAIRMLVNLVVFVILLLAA 163 Query: 403 FLIYVAFNFSTERL 416 IYV FN S L Sbjct: 164 ITIYVLFNVSLAEL 177 Score = 109 bits (262), Expect = 2e-25 Identities = 44/74 (59%), Positives = 54/74 (72%) Query: 525 ECWETYVGQQFYKLILTDFAVQFVTTFLINLPRAFMARHTRSRCLKMFGEQEFYLPKHVL 584 +CWET+VGQQFYKL + DFA F+ TF +N PRA ARH+ SR K GEQEF L KHVL Sbjct: 384 QCWETFVGQQFYKLFIVDFATHFLVTFFVNFPRALFARHSSSRLAKFIGEQEFELSKHVL 443 Query: 585 DIVYVQTIIWMAMF 598 D++Y QT+ W+ F Sbjct: 444 DVIYSQTLCWLGTF 457 Score = 47.6 bits (108), Expect = 1e-06 Identities = 16/37 (43%), Positives = 29/37 (78%) Query: 441 QLENVLMEFLPFICIVVLNLIIPEIFSYMIRYEGYMP 477 +L + EFLP++ IV +NL++P++F+Y+++YE Y P Sbjct: 260 KLRVLFYEFLPYLAIVCMNLVVPQLFNYLVQYEKYSP 296 >AY263175-1|AAP78790.1| 814|Anopheles gambiae TmcA-like protein protein. Length = 814 Score = 69.3 bits (162), Expect = 3e-13 Identities = 73/302 (24%), Positives = 140/302 (46%), Gaps = 35/302 (11%) Query: 97 ENLSNEERSWEEIMQ-IKALPVPMSQKIELKARLQNATKLRLQGFEQLHLRQR---KVWH 152 + L + R +E++Q +K P+ M +K+ L + ++ R +G Q H R + Sbjct: 49 DKLFDTIRLHKEVLQTVKLQPISMKRKLRLVQQAKSYIT-RHEGALQEHFTSRTARSLLA 107 Query: 153 RFRIGCTETFSKL--EL---------WQTPMREIEGKFGTGVVSYXXXXXXXXXXXXTIS 201 +F I T + +L EL W++ ++EIE FG+ V SY IS Sbjct: 108 QFNIFLTTKWQQLLRELANLATYLIPWESRIKEIESHFGSVVASYFTFLRWLFSVNIVIS 167 Query: 202 ILVIVFLILPKTLLTERVYECDETEANSTVCCSIAYFEKNTTNSNIFLDVIQGTGWMERT 261 +L++VF+++P E +Y D +A + +++ E T + F + + G ++ + Sbjct: 168 VLLVVFIMVP-----EEIY-VDPEKAKCDIRKTMSKQELALTRN--FSTIWEFEGHLKYS 219 Query: 262 ILFYGVYTDQIYTYYVNSLYHTKLFYNMPMAYILVPISWTLLSLIAIVKTAARGFKQKLI 321 LFYG Y+ T+ + YN+P+AY + + S +A +K A + + Sbjct: 220 PLFYGYYS----TFSGAIAWG----YNLPLAYFFTGLVVYIYSFVATLKKMAENSRMSKL 271 Query: 322 ESEGQFYKYCNLVFGGWDFCIHNDRSARIKHQALYNEIKGCIEEERIKEEKQSRSRESQI 381 S+ Y + +F GWD+ I + +A+ + ++ K + EE EK+ +R +I Sbjct: 272 SSKDDEYVFSWKLFTGWDYMIGHMETAQNRMASIILGFKEALLEE---AEKKKDTRNWKI 328 Query: 382 LM 383 ++ Sbjct: 329 IL 330 Score = 50.0 bits (114), Expect = 2e-07 Identities = 30/86 (34%), Positives = 46/86 (53%), Gaps = 6/86 (6%) Query: 518 NTDTTKLECWETYVGQQFYKLILTDFAVQFVTTFLINLPRAFMARHTRS-RCL---KMFG 573 N D L CWET GQ+ KL + D V V+T +++ RA R+ S C K F Sbjct: 474 NADIRSL-CWETMFGQELAKLTVMDLLVTIVSTLILDFLRALFVRYMNSCWCWDLEKKFP 532 Query: 574 EQ-EFYLPKHVLDIVYVQTIIWMAMF 598 + +F + +++L +V Q ++WM MF Sbjct: 533 KYGDFKIAENILHLVNNQGMVWMGMF 558 >AY263176-1|AAP78791.1| 705|Anopheles gambiae TmcB-like protein protein. Length = 705 Score = 50.4 bits (115), Expect = 1e-07 Identities = 25/112 (22%), Positives = 56/112 (50%), Gaps = 6/112 (5%) Query: 250 DVIQGTGWMERTILFYGVYTDQIYTYYVNSLYHTKLFYNMPMAYILVPISWTLLSLIAIV 309 D++ G G++ ++++YG Y+++ +T + Y++P AY L L + + + Sbjct: 167 DLLTGEGYLSDSVMYYGSYSNRSFTLVPGTAE-----YSLPHAYFLTITILLLATFVFVS 221 Query: 310 KTAARGFKQKLIESEGQFYKYC-NLVFGGWDFCIHNDRSARIKHQALYNEIK 360 + ++ IES + + WD+ I N ++AR+KH + +E++ Sbjct: 222 VSMGHAYRISFIESSATVQNILTHKIVCSWDYGIANGKAARLKHATILSELR 273 Score = 42.3 bits (95), Expect = 4e-05 Identities = 25/81 (30%), Positives = 41/81 (50%), Gaps = 12/81 (14%) Query: 526 CWETYVGQQFYKLILTDFAVQFVTTFLINLPRA----FMARHTRSR-----CLKMFGEQE 576 CWET +GQ+ Y+L++ DF F++T L++ R AR+ +R Sbjct: 399 CWETAIGQELYRLLVVDF---FISTVLLSAVRGVRYLLHARYGPARGRAGPLAPYLTPPP 455 Query: 577 FYLPKHVLDIVYVQTIIWMAM 597 F + H L +VY QT++W + Sbjct: 456 FCIETHSLGLVYNQTLLWFGV 476 >M93690-1|AAA29364.1| 613|Anopheles gambiae ORF1 protein. Length = 613 Score = 26.2 bits (55), Expect = 2.5 Identities = 25/126 (19%), Positives = 51/126 (40%), Gaps = 5/126 (3%) Query: 28 ELYPGGEQELFDNLQRADAAKLATLLPSKQARNTTTVKRARSQTDRRQ----STFARTMQ 83 E Y G + + + Q+A AA + T + +RN + + Q R+Q + Q Sbjct: 178 ETYRMGARSVIELQQQAAAAPMMTAQGAHSSRNRRGRQGPQQQEQRQQQQQHQQREQQQQ 237 Query: 84 SRDIHLSMLPDLSENLSNEERSWEEIMQIKALPVPMSQKIELKARLQNATKLRLQGFEQL 143 + + N++R W++ Q + Q+ + + + QN R Q +Q Sbjct: 238 QQQQQQQQQQQQQQQQRNQQREWQQ-QQQQQQHQQREQQQQQRVQQQNQQHQRQQQQQQQ 296 Query: 144 HLRQRK 149 +Q++ Sbjct: 297 QRQQQQ 302 >AY391746-1|AAR28996.1| 502|Anopheles gambiae putative GPCR protein. Length = 502 Score = 25.4 bits (53), Expect = 4.4 Identities = 12/23 (52%), Positives = 16/23 (69%) Query: 439 FNQLENVLMEFLPFICIVVLNLI 461 FN L+ +L+ +PF IVVLN I Sbjct: 284 FNFLDFILVFVVPFTIIVVLNTI 306 >AY391745-1|AAR28995.1| 460|Anopheles gambiae putative GPCR protein. Length = 460 Score = 25.4 bits (53), Expect = 4.4 Identities = 10/23 (43%), Positives = 16/23 (69%) Query: 439 FNQLENVLMEFLPFICIVVLNLI 461 FN ++ V++ +PF IVVLN + Sbjct: 203 FNYIDTVIVFVVPFTIIVVLNSV 225 >AY056833-1|AAL23627.1| 1253|Anopheles gambiae chitin synthase protein. Length = 1253 Score = 25.4 bits (53), Expect = 4.4 Identities = 13/43 (30%), Positives = 26/43 (60%), Gaps = 2/43 (4%) Query: 100 SNEERSWEEIMQ--IKALPVPMSQKIELKARLQNATKLRLQGF 140 ++EE+ W E+++ +K L + QK E+K++L+ L + F Sbjct: 886 ASEEQFWIELIEKYLKPLDLSEKQKEEMKSQLKGLRDLAVFAF 928 >AF533893-1|AAM97678.1| 570|Anopheles gambiae ascorbate transporter protein. Length = 570 Score = 25.0 bits (52), Expect = 5.9 Identities = 11/27 (40%), Positives = 15/27 (55%), Gaps = 1/27 (3%) Query: 159 TETFSKLELWQTPMREIEGKFGTGVVS 185 T+T + ELWQ MRE+ G V+ Sbjct: 134 TDT-DRTELWQVRMRELSGAIAVAAVT 159 Database: mosquito Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 516,269 Number of sequences in database: 2123 Lambda K H 0.324 0.137 0.414 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 557,415 Number of Sequences: 2123 Number of extensions: 21427 Number of successful extensions: 71 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 55 Number of HSP's gapped (non-prelim): 13 length of query: 612 length of database: 516,269 effective HSP length: 68 effective length of query: 544 effective length of database: 371,905 effective search space: 202316320 effective search space used: 202316320 T: 11 A: 40 X1: 15 ( 7.0 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.6 bits) S2: 51 (24.6 bits)
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