Silkworm BLAST Result against bee

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000065-TA|BGIBMGA000065-PA|IPR001216|Cysteine
synthase/cystathionine beta-synthase P-phosphate-binding site,
IPR001926|Pyridoxal-5'-phosphate-dependent enzyme, beta subunit
         (440 letters)

Database: bee 
           429 sequences; 140,377 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB244761-1|BAE66603.1|  504|Apis mellifera cystathionine beta-sy...   329   3e-92
AF388659-1|AAK71995.1|  782|Apis mellifera 1D-myo-inositol-trisp...    25   1.7  
DQ435328-1|ABD92643.1|  143|Apis mellifera OBP11 protein.              23   6.7  
AY463910-1|AAR24352.1|  843|Apis mellifera metabotropic glutamat...    22   8.9  
AB253416-1|BAE86927.1|  580|Apis mellifera alpha-glucosidase pro...    22   8.9  
AB161181-1|BAD08343.1|  933|Apis mellifera metabotropic glutamat...    22   8.9  

>AB244761-1|BAE66603.1|  504|Apis mellifera cystathionine
           beta-synthase protein.
          Length = 504

 Score =  329 bits (808), Expect = 3e-92
 Identities = 161/326 (49%), Positives = 219/326 (67%), Gaps = 7/326 (2%)

Query: 11  RIYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSG 70
           +I  +IL  IG TPL++LN IPK YG++CE++AKCEF+NPGGS+KDRI+Y M+ +A+  G
Sbjct: 34  KIMPDILTAIGQTPLIKLNNIPKSYGIKCEIYAKCEFLNPGGSVKDRIAYRMIQDAEDKG 93

Query: 71  IAKSGTRFIEPTSGNTGIGVALATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQTPAM 130
           + K G   IEPTSGNTGIG+A+A AV G+ C IV P+K SDEK+ST+  LGA++++TP  
Sbjct: 94  LLKPGCTIIEPTSGNTGIGLAMAAAVRGYKCIIVMPEKMSDEKISTLYALGAKIIRTPTE 153

Query: 131 APWESSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTAEEILALVP-DVDMIXXX 189
           A W S E  +SVA++   E PN+I  DQY N  NP  HY+ TA EI       +D +   
Sbjct: 154 ASWHSPEAHISVAQKLQKEIPNSIILDQYTNPGNPLAHYDQTAIEIWKQCEGKIDYLVAG 213

Query: 190 XXXXXXXXXXAQKVKEKCPKCIIVTVDPQGSLI----FGEGPADLYFVEGIGGDFVPDVL 245
                      +K+KE  P   I+ VDP+GS++      +     Y VEGIG DF+P VL
Sbjct: 214 AGTGGTISGIGRKLKELSPNIKIIAVDPKGSILDPSSDSQNEVGFYEVEGIGYDFIPTVL 273

Query: 246 DKSVIDKVEKPNDYDAFNMSREIIKKEGLLCGGSSGAAMISAIRAAKKLNFGAGKRVVVI 305
           D++VIDK  K  D ++ N +R +I++EGLLCGGSSGAA+I+A++ AK  +    KR+V+I
Sbjct: 274 DRNVIDKWIKTEDNESLNAARMLIRQEGLLCGGSSGAALIAALKIAK--DIPEEKRMVII 331

Query: 306 LPDGIRNYMTKFVCDQWMEAHLFKEP 331
           LPDGIRNY+TKFV + WME   F +P
Sbjct: 332 LPDGIRNYLTKFVSEYWMETRGFLQP 357



 Score = 34.3 bits (75), Expect = 0.002
 Identities = 25/100 (25%), Positives = 56/100 (56%), Gaps = 16/100 (16%)

Query: 337 LFFKGVLTKDSLRSEATNPKKAGRIDFNEPVNRHLIKTYYKIVENRGNPTVGLASRILDI 396
           +  KGV++ + L S   +    G +   + V++ ++K Y K+   + + T+G  SR+L+ 
Sbjct: 412 IHIKGVISLNKLTSYVIS----GIVKCTDFVDKAMVKQYVKV---KHSATLGYISRVLEK 464

Query: 397 APFVIIVEAYYENSNKKQFKGYIPKGIVTTEHVMEYILEH 436
            P+VII++  ++++       +I  GIV   H++++I ++
Sbjct: 465 EPYVIILDDEHDDA-------FI--GIVNQFHILQFITKN 495


>AF388659-1|AAK71995.1|  782|Apis mellifera
           1D-myo-inositol-trisphosphate 3-kinaseisoform A protein.
          Length = 782

 Score = 24.6 bits (51), Expect = 1.7
 Identities = 9/18 (50%), Positives = 11/18 (61%)

Query: 404 EAYYENSNKKQFKGYIPK 421
           E YY   N +QF  YIP+
Sbjct: 384 ETYYSKHNTQQFTQYIPE 401


>DQ435328-1|ABD92643.1|  143|Apis mellifera OBP11 protein.
          Length = 143

 Score = 22.6 bits (46), Expect = 6.7
 Identities = 15/75 (20%), Positives = 24/75 (32%)

Query: 9   RNRIYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQK 68
           R +       TI D         P+D  L+C      E  N       +I Y ++ +   
Sbjct: 40  RKKCIGETKTTIEDVEATEYGEFPEDEKLKCYFNCVLEKFNVMDKKNGKIRYNLLKKVIP 99

Query: 69  SGIAKSGTRFIEPTS 83
               + G   I+  S
Sbjct: 100 EAFKEIGVEMIDSCS 114


>AY463910-1|AAR24352.1|  843|Apis mellifera metabotropic glutamate
           receptor 1 protein.
          Length = 843

 Score = 22.2 bits (45), Expect = 8.9
 Identities = 9/30 (30%), Positives = 18/30 (60%)

Query: 6   SFTRNRIYNNILETIGDTPLVRLNRIPKDY 35
           SF  +++YN +L  I     V+  +IP+++
Sbjct: 673 SFLFSQLYNALLILISTVYAVKTRKIPENF 702


>AB253416-1|BAE86927.1|  580|Apis mellifera alpha-glucosidase
           protein.
          Length = 580

 Score = 22.2 bits (45), Expect = 8.9
 Identities = 11/30 (36%), Positives = 17/30 (56%), Gaps = 1/30 (3%)

Query: 146 RLLEDPNAISCDQYKNDVNPRTHYEYTAEE 175
           RLL++P+A   D  K+D     H  YT ++
Sbjct: 233 RLLDEPSANRTDLSKDDYESLVHL-YTRDQ 261


>AB161181-1|BAD08343.1|  933|Apis mellifera metabotropic glutamate
           receptor protein.
          Length = 933

 Score = 22.2 bits (45), Expect = 8.9
 Identities = 9/30 (30%), Positives = 18/30 (60%)

Query: 6   SFTRNRIYNNILETIGDTPLVRLNRIPKDY 35
           SF  +++YN +L  I     V+  +IP+++
Sbjct: 763 SFLFSQLYNALLILISTVYAVKTRKIPENF 792


  Database: bee
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 140,377
  Number of sequences in database:  429
  
Lambda     K      H
   0.318    0.137    0.402 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 128,676
Number of Sequences: 429
Number of extensions: 5362
Number of successful extensions: 13
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3
Number of HSP's gapped (non-prelim): 8
length of query: 440
length of database: 140,377
effective HSP length: 60
effective length of query: 380
effective length of database: 114,637
effective search space: 43562060
effective search space used: 43562060
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 45 (22.2 bits)