Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000065-TA|BGIBMGA000065-PA|IPR001216|Cysteine
synthase/cystathionine beta-synthase P-phosphate-binding site,
IPR001926|Pyridoxal-5'-phosphate-dependent enzyme, beta subunit
(440 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces po... 167 3e-42
SPAC3A12.17c |cys12|cys1b|cysteine synthase Cys12|Schizosaccharo... 120 4e-28
SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces pomb... 44 3e-05
SPAC922.03 |||1-aminocyclopropane-1-carboxylate deaminase |Schiz... 33 0.10
SPCC320.14 ||SPCC330.15c|threo-3-hydroxyaspartate ammonia-lyase ... 33 0.10
SPBC12D12.05c |||mitochondrial carrier, calcium binding subfamil... 28 2.3
SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c... 28 3.0
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 27 5.2
>SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 351
Score = 167 bits (406), Expect = 3e-42
Identities = 118/341 (34%), Positives = 170/341 (49%), Gaps = 20/341 (5%)
Query: 12 IYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGI 71
I + + IG TPL+RLN + + G C + AK EF NPGGS+KDR +Y +V +A+K G
Sbjct: 13 IVSGFIGAIGRTPLIRLNTLSNETG--CNILAKAEFQNPGGSVKDRAAYYVVRDAEKKGK 70
Query: 72 AKSGTRFIEPTSGNTGIGVALATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQTPAMA 131
G +E T+GNTGIG+A G+ C I P+ S K+ T+ LGAEV P +A
Sbjct: 71 LSRGGTIVEGTAGNTGIGLAHIARARGYKCVIYMPNTQSQAKIDTLKFLGAEVHPVP-VA 129
Query: 132 PWESSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTAEEILALVP-DVDMIXXXX 190
P+ + ++ A+R PNA DQ+ N N +HYE T EI VD
Sbjct: 130 PFSNPLNYNHQARRHAESTPNASWTDQFDNVANLLSHYETTGPEIWDQTKGTVDGFTCST 189
Query: 191 XXXXXXXXXAQKVKEKCP-KCIIVTVDPQGSLIF------GEGPAD--LYFVEGIG-GDF 240
+ +KEK + DP GS+++ G+ P + F EGIG G
Sbjct: 190 GTGGTFAGVTKYLKEKSDGRVASFVADPPGSVLYSHIKTKGKHPDNKGSSFTEGIGQGRI 249
Query: 241 VPDVLD-KSVIDKVEKPNDYDAFNMSREIIKKEGLLCGGSSGAAMISAIRAAKKLNFGAG 299
+V +ID K D + NM ++ +EGL GGSS +++A+ AK L G G
Sbjct: 250 TGNVQPVYDLIDDAMKIPDEKSINMFFRLLDQEGLFLGGSSCLNVVAAVEMAKIL--GPG 307
Query: 300 KRVVVILPDGIRNYMTKFVCDQWMEA-HLFK--EPPEREFL 337
K VV IL D Y T+ ++E+ LF EP ++++
Sbjct: 308 KTVVTILCDSGHKYATRLFSRSFLESKKLFDVIEPQYKKYI 348
>SPAC3A12.17c |cys12|cys1b|cysteine synthase
Cys12|Schizosaccharomyces pombe|chr 1|||Manual
Length = 395
Score = 120 bits (289), Expect = 4e-28
Identities = 94/341 (27%), Positives = 153/341 (44%), Gaps = 34/341 (9%)
Query: 12 IYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSG- 70
I N + IG+T +VR+ + + G C++ AK EF+NPG S KDR++ M+ A+++G
Sbjct: 42 IVNGVEGLIGNTKMVRIKSLSQATG--CDILAKAEFLNPGNSPKDRVALQMIRTAEENGD 99
Query: 71 -IAKSGTRFIEPTSGNTGIGVALATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQTPA 129
+ E T+G+TGI +A+ G+ I P S EK + LLGA VQ
Sbjct: 100 LVPYQSNAVYEGTAGSTGISIAMLCCSLGYDSRIYMPSDQSKEKSDILELLGAH-VQRVT 158
Query: 130 MAPWESSEHFLSVAKRRLLEDP-------NAISCDQYKNDVNPRTHYEYTAEEI-LALVP 181
AP HF++ A+R +Q++N N + H+ T EI
Sbjct: 159 PAPIVDPNHFVNTARRNAANHTVDESIPGKGYFANQFENPANWQAHFNSTGPEIWRQCAG 218
Query: 182 DVDMIXXXXXXXXXXXXXAQKVKEKCPKCIIVTVDPQGSLIF-------------GEGPA 228
+D ++ +K K P + DP GS ++ EG
Sbjct: 219 KLDAFIAGSGTGGTIAGISRYLKSKDPSITVCLADPPGSGLYHKVLHGVMFDLAEREGTR 278
Query: 229 DLY----FVEGIGGDFVPD--VLDKSVIDKVEKPNDYDAFNMSREIIKKEGLLCGGSSGA 282
+ VEG+G + + + + +ID + D A MSR ++ +GL G SS
Sbjct: 279 RRHQVDTIVEGVGINRMTRNFSIAEPLIDMAYRVTDEQAVAMSRYLVTHDGLFVGSSSAV 338
Query: 283 AMISAIRAAKKLNFGAGKRVVVILPDGIRNYMTKFVCDQWM 323
++A+R AKKL G G R+V +L D + +K ++++
Sbjct: 339 NCVAAVRLAKKL--GPGHRIVTLLCDPGSRHFSKLYNEEFL 377
>SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 600
Score = 44.4 bits (100), Expect = 3e-05
Identities = 34/119 (28%), Positives = 56/119 (47%), Gaps = 5/119 (4%)
Query: 7 FTRNRIYNNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEA 66
+ R + +N+ E I +TPL + I + G+ ++ K E + P S K R ++ +
Sbjct: 98 YLRLTLTSNVYEVIKETPLTKGVVISESTGVP--VYLKREDLTPVFSFKIRGAHNKMASL 155
Query: 67 QKSGIAKSGTRFIEPTSGNTGIGVALATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVV 125
K + K+G I ++GN GVA + G TIV P + K + LGA V+
Sbjct: 156 DKQSL-KNGV--IACSAGNHAQGVAYSARTLGVKATIVMPQNTPEIKWRNVKRLGANVL 211
>SPAC922.03 |||1-aminocyclopropane-1-carboxylate deaminase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 338
Score = 32.7 bits (71), Expect = 0.10
Identities = 28/92 (30%), Positives = 38/92 (41%), Gaps = 5/92 (5%)
Query: 19 TIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPG---GSIKDRISYTMVLEAQKSGIAKSG 75
T G TP+ + R+ K G + E+FAK E N G G K R ++ EA G
Sbjct: 12 TFGPTPITSMKRLSKTLGGKVEIFAKREDCNSGLAFGGNKIRKLEYLIPEAIDGGYDTLV 71
Query: 76 TRFIEPTSGNTGIGVALATAVCGFGCTIVTPD 107
+ I N VA A G C ++ D
Sbjct: 72 S--IGGIQSNQTRQVAAVAAHLGLDCVLIQED 101
>SPCC320.14 ||SPCC330.15c|threo-3-hydroxyaspartate ammonia-lyase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 323
Score = 32.7 bits (71), Expect = 0.10
Identities = 24/103 (23%), Positives = 49/103 (47%), Gaps = 5/103 (4%)
Query: 23 TPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGIAKSGTRFIEPT 82
TP++ + + K++ E+F KCE G+ K R + + + ++ K+G + +
Sbjct: 27 TPVLTSSTVNKEF--VAEVFFKCENFQKMGAFKFRGALNALSQLNEAQ-RKAGV--LTFS 81
Query: 83 SGNTGIGVALATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVV 125
SGN +AL+ + G I+ P + K++ G +V+
Sbjct: 82 SGNHAQAIALSAKILGIPAKIIMPLDAPEAKVAATKGYGGQVI 124
>SPBC12D12.05c |||mitochondrial carrier, calcium binding
subfamily|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 28.3 bits (60), Expect = 2.3
Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 2/43 (4%)
Query: 372 IKTYYKIVENRGNPTV--GLASRILDIAPFVIIVEAYYENSNK 412
I +YK +N G + GL+ +L +AP V I YEN K
Sbjct: 379 IDCFYKTTKNEGFRGLYKGLSPNLLKVAPSVAISYLVYENCKK 421
>SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1315
Score = 27.9 bits (59), Expect = 3.0
Identities = 24/108 (22%), Positives = 49/108 (45%), Gaps = 6/108 (5%)
Query: 311 RNYMTKFVCDQWMEAHLFKEPPEREFLFFKGVLT-KDSLRSEATNPKKAGRIDFNEPVNR 369
RN ++ D E +EP E + + DSL S+ +N A ++D + + R
Sbjct: 892 RNQALSYLVDGMPENDPRREPFESRTKCYSYIFEILDSLESQMSNDSSAIKVDVYDTIQR 951
Query: 370 HLIKTYYKIVENRGNPTVGLASRILDI-APFVIIVEAYYENSNKKQFK 416
+ ++ + GL R+++I +P+ +++Y E ++ K K
Sbjct: 952 SKDELFHYCFYD-WYSFKGLTDRLIEIDSPY---IQSYLERNSTKDMK 995
>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2104
Score = 27.1 bits (57), Expect = 5.2
Identities = 24/89 (26%), Positives = 45/89 (50%), Gaps = 10/89 (11%)
Query: 357 KAGRIDFNEPVNR--HLIKTYYKIVENRGNPT---VGLASRILDIAPFVIIVEAYYENSN 411
K+G +D N+ +++ LI++ K E N T +G ++LD AP + V N+
Sbjct: 1471 KSGILD-NKDLSKFSELIQSLLKENEELKNLTTSNLGSDDKMLDFAPLLEDVP----NNT 1525
Query: 412 KKQFKGYIPKGIVTTEHVMEYILEHQDKL 440
+ Q KG++ K I + + + ++KL
Sbjct: 1526 RNQIKGFVEKAISSKRAIAKLYSASEEKL 1554
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.137 0.402
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,028,861
Number of Sequences: 5004
Number of extensions: 85014
Number of successful extensions: 173
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 162
Number of HSP's gapped (non-prelim): 10
length of query: 440
length of database: 2,362,478
effective HSP length: 75
effective length of query: 365
effective length of database: 1,987,178
effective search space: 725319970
effective search space used: 725319970
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 55 (26.2 bits)
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