BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000065-TA|BGIBMGA000065-PA|IPR001216|Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site, IPR001926|Pyridoxal-5'-phosphate-dependent enzyme, beta subunit (440 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_2393| Best HMM Match : No HMM Matches (HMM E-Value=.) 318 4e-87 SB_32365| Best HMM Match : PALP (HMM E-Value=0.064) 114 1e-25 SB_18116| Best HMM Match : PALP (HMM E-Value=0) 114 1e-25 SB_51924| Best HMM Match : PALP (HMM E-Value=3.1e-05) 38 0.021 SB_2970| Best HMM Match : No HMM Matches (HMM E-Value=.) 37 0.028 SB_44496| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.11 SB_43519| Best HMM Match : Pre-SET (HMM E-Value=0.37) 31 1.4 SB_38968| Best HMM Match : RNA_POL_M_15KD (HMM E-Value=0.78) 31 2.5 SB_2329| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 2.5 SB_45280| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 9.9 >SB_2393| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 384 Score = 318 bits (782), Expect = 4e-87 Identities = 162/306 (52%), Positives = 206/306 (67%), Gaps = 8/306 (2%) Query: 15 NILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGIAKS 74 NIL+ IGDTPLVR+N I K YGL+CE+ AKCEF N GGS+KDRI M+ EA++ G K Sbjct: 3 NILQKIGDTPLVRINNIGKTYGLKCELLAKCEFFNAGGSVKDRIGLRMIEEAEREGRLKP 62 Query: 75 GTRFIEPTSGNTGIGVALATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQTPAMAPWE 134 G IEPTSGNTGIG+ALA AV G+ C IV P+K S+EK+ + LGAE+V+TP A ++ Sbjct: 63 GDTLIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSNEKVDVLRALGAEIVRTPTEAKFD 122 Query: 135 SSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTAEEILALVP-DVDMIXXXXXXX 193 S E + VA+R E N+ DQY+N NP HY+ TA+EIL VDM+ Sbjct: 123 SPESHIGVAQRLNAEIANSHILDQYRNPGNPLAHYDSTADEILQQCDGKVDMVVLGAGTG 182 Query: 194 XXXXXXAQKVKEKCPKCIIVTVDPQGSLI-----FGEGPADLYFVEGIGGDFVPDVLDKS 248 A+K+KEKCP IV VDP+GS++ E +Y VEGIG DFVP VLD+S Sbjct: 183 GTITGIARKLKEKCPGVTIVGVDPKGSILAEPESLNESDVSMYHVEGIGYDFVPTVLDRS 242 Query: 249 VIDKVEKPNDYDAFNMSREIIKKEGLLCGGSSGAAMISAIRAAKKLNFGAGKRVVVILPD 308 V+DK K D +F MSR +I++EGLLCGGSSG+AM++A+ AAK LN G+R VVILPD Sbjct: 243 VVDKWYKSEDKTSFTMSRRLIREEGLLCGGSSGSAMVAAVEAAKSLN--EGQRCVVILPD 300 Query: 309 GIRNYM 314 +RNY+ Sbjct: 301 SVRNYI 306 >SB_32365| Best HMM Match : PALP (HMM E-Value=0.064) Length = 129 Score = 114 bits (275), Expect = 1e-25 Identities = 58/126 (46%), Positives = 80/126 (63%) Query: 33 KDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGIAKSGTRFIEPTSGNTGIGVAL 92 K GL+CE+ AKCEF+NP GS+KDRI M+ +A++ K G IEPTSGNTGI +AL Sbjct: 4 KRCGLKCEVVAKCEFMNPVGSLKDRIGVRMIEDAERDNHIKPGDTLIEPTSGNTGIALAL 63 Query: 93 ATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQTPAMAPWESSEHFLSVAKRRLLEDPN 152 A+AV G+ C IV +K S +K T LG V+ PA A ++S E + +A++ E PN Sbjct: 64 ASAVKGYRCVIVMSEKMSSDKADTQKALGGICVRAPAEAKFDSPESTIRLAQQLQKEIPN 123 Query: 153 AISCDQ 158 + +Q Sbjct: 124 SYVLNQ 129 >SB_18116| Best HMM Match : PALP (HMM E-Value=0) Length = 470 Score = 114 bits (275), Expect = 1e-25 Identities = 58/126 (46%), Positives = 80/126 (63%) Query: 33 KDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGIAKSGTRFIEPTSGNTGIGVAL 92 K GL+CE+ AKCEF+NP GS+KDRI M+ +A++ K G IEPTSGNTGI +AL Sbjct: 4 KRCGLKCEVVAKCEFMNPVGSLKDRIGVRMIEDAERDNHIKPGDTLIEPTSGNTGIALAL 63 Query: 93 ATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQTPAMAPWESSEHFLSVAKRRLLEDPN 152 A+AV G+ C IV +K S +K T LG V+ PA A ++S E + +A++ E PN Sbjct: 64 ASAVKGYRCVIVMSEKMSSDKADTQKALGGICVRAPAEAKFDSPESTIRLAQQLQKEIPN 123 Query: 153 AISCDQ 158 + +Q Sbjct: 124 SYVLNQ 129 Score = 99 bits (238), Expect = 4e-21 Identities = 86/292 (29%), Positives = 142/292 (48%), Gaps = 55/292 (18%) Query: 183 VDMIXXXXXXXXXXXXXAQKVKEKCPKCIIVTVDPQGSLIFGEGPADL---------YFV 233 +DM+ A+K++EK P IV VDP GS++ P +L Y V Sbjct: 192 LDMLVIGTGTGGTLTGVARKIREKLPHVKIVAVDPYGSVL--AVPEELNKTEPGFKGYNV 249 Query: 234 EGIGGDFVPDVLDKSVIDKVEKPNDYDAFNMSREIIKKEGLLCGGSSGAAMISAIRAAKK 293 EGIG +VP VLD+ ++D+ +K D +AF ++ + K+EGLL GGSSG+ M +A+RAA+ Sbjct: 250 EGIGKPYVPVVLDRDLVDEWQKIGDKEAFYFAKRLSKEEGLLVGGSSGSVMAAAVRAAQD 309 Query: 294 LNFGAGKRVVVILPDGIRNYM--------------TKFVCDQWMEAHLFK-------EPP 332 L+ +R VV+ D IRNYM +KFV D+W+E + +P Sbjct: 310 LD--ENQRCVVVFADSIRNYMYVPNRIVAWQKKIWSKFVSDEWLEERNLQHDDINDNDPQ 367 Query: 333 EREFLFFKGVLT--KDSLRSEATN-----PKKAGRIDFNEPVNRHLIKTYYKIVENRGNP 385 L K +L + + T+ + AGR+D + + + + K + ++ + Sbjct: 368 HCSNLTVKDLLAFFSNGILGIVTHGNLLASEAAGRVDMSSTLEKLIYKKFDQVSRDTSLE 427 Query: 386 TVGLASRILDIAPFVIIVEAYYENSNKKQFKGYIPKGIVTTEHVMEYILEHQ 437 V SR LD FV++ + ++F +VT H++ +I+E + Sbjct: 428 KV---SRCLDNDYFVLV-----PGCDSQEF------SVVTRGHLLNFIIERE 465 >SB_51924| Best HMM Match : PALP (HMM E-Value=3.1e-05) Length = 407 Score = 37.5 bits (83), Expect = 0.021 Identities = 31/118 (26%), Positives = 51/118 (43%), Gaps = 7/118 (5%) Query: 14 NNILETIGDTPLVRLNRIPKDYGLECEMFAKCEFVNPGGSIKDRISYTMVLEAQKSGIAK 73 + I I TP++ + + K G +F KCE + G+ K R + VL +S Sbjct: 106 DRIAPYINSTPVMTSHTLDKMAGRS--LFFKCENLQKAGAFKFRGAMNAVLRLVESSDDP 163 Query: 74 SGTRFIEP-----TSGNTGIGVALATAVCGFGCTIVTPDKNSDEKMSTISLLGAEVVQ 126 SG++ P +SGN ++LA + IV P K + + GA +V+ Sbjct: 164 SGSKSQAPCVVTHSSGNHAQALSLAAKLMNLKAHIVMPKNAPHVKKAAVIEYGANIVE 221 >SB_2970| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 85 Score = 37.1 bits (82), Expect = 0.028 Identities = 20/63 (31%), Positives = 31/63 (49%), Gaps = 1/63 (1%) Query: 114 MSTISLLGAEVVQTPAMAPWESSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTA 173 M + LGAEV P + P++ ++ A+R N + +Q+ N N R H+E T Sbjct: 1 MDLLKALGAEVRPVPVV-PFDDQMNYNHQARRYAESIENGVWTNQFDNTANRRAHFETTG 59 Query: 174 EEI 176 EI Sbjct: 60 PEI 62 >SB_44496| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 27 Score = 35.1 bits (77), Expect = 0.11 Identities = 16/26 (61%), Positives = 20/26 (76%) Query: 88 IGVALATAVCGFGCTIVTPDKNSDEK 113 IG+ALA AV G+ C IV P+K S+EK Sbjct: 2 IGLALAAAVKGYRCIIVMPEKMSNEK 27 >SB_43519| Best HMM Match : Pre-SET (HMM E-Value=0.37) Length = 829 Score = 31.5 bits (68), Expect = 1.4 Identities = 19/74 (25%), Positives = 33/74 (44%), Gaps = 2/74 (2%) Query: 101 CTIVTPDKNSDEKMSTISLLGAEVVQTPAMAPWESSEHFLSVAKRRLLEDPNAISCDQYK 160 C + PD +SD ST+S+ + P EH + K ++ + S D+Y+ Sbjct: 163 CVVFDPDNDSDS--STLSVFLERYMSDGWEGPEPYKEHHVINRKEWVVTSDDGSSEDRYE 220 Query: 161 NDVNPRTHYEYTAE 174 +DV+ Y +E Sbjct: 221 DDVSSANELGYASE 234 >SB_38968| Best HMM Match : RNA_POL_M_15KD (HMM E-Value=0.78) Length = 152 Score = 30.7 bits (66), Expect = 2.5 Identities = 17/47 (36%), Positives = 26/47 (55%) Query: 131 APWESSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTAEEIL 177 A +E SE + S+ RR+L + + I CDQ+K+ + Y EE L Sbjct: 34 ACYEISEGYGSLQNRRVLGEVSMIFCDQHKSLKKSGATWSYEEEEEL 80 >SB_2329| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1181 Score = 30.7 bits (66), Expect = 2.5 Identities = 17/47 (36%), Positives = 26/47 (55%) Query: 131 APWESSEHFLSVAKRRLLEDPNAISCDQYKNDVNPRTHYEYTAEEIL 177 A +E SE + S+ RR+L + + I CDQ+K+ + Y EE L Sbjct: 912 ACYEISEGYGSLQNRRVLGEVSMIFCDQHKSLKKSGATWSYEEEEEL 958 >SB_45280| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 106 Score = 28.7 bits (61), Expect = 9.9 Identities = 19/68 (27%), Positives = 31/68 (45%), Gaps = 5/68 (7%) Query: 315 TKFVCDQWMEAHLFKEPPEREFLFFKGVLTKDSLRSEAT----NPKKAGRIDFNEPVNR- 369 T +C QW + K+P + + L K +L + + T NPK A ++ NR Sbjct: 24 TNIICGQWYHKYRKKKPRDAKVLNIKELLEEGEANEKVTESEANPKPAINTEWAHSENRF 83 Query: 370 HLIKTYYK 377 + +KT K Sbjct: 84 NSLKTAEK 91 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.137 0.402 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,658,380 Number of Sequences: 59808 Number of extensions: 597459 Number of successful extensions: 1576 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 1562 Number of HSP's gapped (non-prelim): 11 length of query: 440 length of database: 16,821,457 effective HSP length: 84 effective length of query: 356 effective length of database: 11,797,585 effective search space: 4199940260 effective search space used: 4199940260 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 61 (28.7 bits)
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