Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000034-TA|BGIBMGA000034-PA|IPR005225|Small GTP-binding
protein domain, IPR006689|ARF/SAR superfamily,
IPR006688|ADP-ribosylation factor, IPR006687|GTP-binding protein SAR1,
IPR001806|Ras GTPase
(197 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 153 2e-38
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 138 7e-34
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 135 5e-33
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 77 2e-15
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 39 4e-04
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 36 0.005
SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 35 0.009
SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr... 33 0.037
SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 33 0.037
SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 32 0.065
SPAC23H4.07c |srp102||signal recognition particle receptor beta ... 30 0.26
SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 28 0.80
SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces ... 28 0.80
SPBC31F10.07 |||cortical component Lsb5 |Schizosaccharomyces pom... 28 1.1
SPAC56E4.06c |ggt2||gamma-glutamyltranspeptidase Ggt2|Schizosacc... 27 1.9
SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||... 27 2.5
SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|ch... 26 4.3
SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr... 25 7.5
SPBPB2B2.01 |||amino acid permease, unknown 12|Schizosaccharomyc... 25 9.9
>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 180
Score = 153 bits (371), Expect = 2e-38
Identities = 72/152 (47%), Positives = 95/152 (62%)
Query: 42 DNAGKTTLLKQLASEDVNHVIPTAGFNIKSVLSNGFKLNVWDIGGQRKIRPYWRNYFENT 101
D AGKTT+L +L ++ IPT GFN+++V VWD+GGQ KIRP WR+YF+NT
Sbjct: 26 DAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNT 85
Query: 102 DILIYVVDCSDHHRXXXXXXXXXXXXXDDKLRAVPLLVYANKQDLATALPASEIAQHLGL 161
+I+VVD +D R +D+LR LLV+ANKQDL A+ A+EI LGL
Sbjct: 86 QGIIFVVDSNDRERISEAHEELQRMLNEDELRDALLLVFANKQDLPNAMNAAEITDKLGL 145
Query: 162 HLIRDRTWQIQACVATDGTGVKEGMEWVCKNI 193
H +R R W IQA AT G G+ EG+EW+ N+
Sbjct: 146 HSLRHRQWYIQATCATSGDGLYEGLEWLSTNL 177
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 138 bits (333), Expect = 7e-34
Identities = 74/181 (40%), Positives = 97/181 (53%), Gaps = 2/181 (1%)
Query: 16 FHGLLDILKKLRSNPEKEXXXXXXXXDNAGKTTLLKQLASEDVNHVIPTAGFNIKSVLSN 75
F G +L SN KE D AGKTT+L +L IPT GFN+++V
Sbjct: 6 FKGFSKPFSRLFSN--KEMRILMLGLDAAGKTTILYKLKLNQSVVTIPTVGFNVETVTYK 63
Query: 76 GFKLNVWDIGGQRKIRPYWRNYFENTDILIYVVDCSDHHRXXXXXXXXXXXXXDDKLRAV 135
K NVWD+GGQ KIRP WR+YF T LI+VVD +D +R D ++R
Sbjct: 64 NIKFNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDSADSNRISEARQELHRIISDREMRDC 123
Query: 136 PLLVYANKQDLATALPASEIAQHLGLHLIRDRTWQIQACVATDGTGVKEGMEWVCKNINV 195
LLV ANKQDL AL ++I L L ++DR W +Q A G G+ EG+ W+ +N +
Sbjct: 124 LLLVLANKQDLPGALSPAQITDVLQLDKLKDRLWNVQPTCALTGDGLLEGLAWLSQNAKL 183
Query: 196 K 196
K
Sbjct: 184 K 184
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 135 bits (326), Expect = 5e-33
Identities = 66/176 (37%), Positives = 103/176 (58%), Gaps = 1/176 (0%)
Query: 18 GLLDILKKLRSNPEKEXXXXXXXXDNAGKTTLLKQLASEDVNHVIPTAGFNIKSVLSNGF 77
GLL IL++ + E+E DNAGKTT+LK L +EDVN V PT GF I+++ G
Sbjct: 2 GLLTILRQQKLK-EREVRVLLLGLDNAGKTTILKCLLNEDVNEVSPTFGFQIRTLEVEGL 60
Query: 78 KLNVWDIGGQRKIRPYWRNYFENTDILIYVVDCSDHHRXXXXXXXXXXXXXDDKLRAVPL 137
+ +WDIGGQ+ +R +W+NYFE+T+ +I+VVD D R ++KL +
Sbjct: 61 RFTIWDIGGQKTLRNFWKNYFESTEAIIWVVDSLDDLRLEECRNTLQELLVEEKLLFTSI 120
Query: 138 LVYANKQDLATALPASEIAQHLGLHLIRDRTWQIQACVATDGTGVKEGMEWVCKNI 193
LV ANK D++ AL + EI++ L + + W+I + A G +K+ + W+ ++
Sbjct: 121 LVLANKSDVSGALSSEEISKILNISKYKSSHWRIFSVSALTGLNIKDAISWLANDL 176
>SPBC31F10.06c |sar1||ADP-ribosylation factor
Sar1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 190
Score = 76.6 bits (180), Expect = 2e-15
Identities = 38/121 (31%), Positives = 60/121 (49%)
Query: 42 DNAGKTTLLKQLASEDVNHVIPTAGFNIKSVLSNGFKLNVWDIGGQRKIRPYWRNYFENT 101
DNAGKTTLL L ++ + + PT + + + +D+GG ++ R WR+YF
Sbjct: 29 DNAGKTTLLHMLKNDRLAVMQPTLHPTSEELAIGNVRFTTFDLGGHQQARRLWRDYFPEV 88
Query: 102 DILIYVVDCSDHHRXXXXXXXXXXXXXDDKLRAVPLLVYANKQDLATALPASEIAQHLGL 161
+ ++Y+VDC D R ++L VP L+ NK D A+ E+ LGL
Sbjct: 89 NGIVYLVDCCDFERLSESKAELDALLAMEELARVPFLILGNKIDAPGAISEDELKAALGL 148
Query: 162 H 162
+
Sbjct: 149 Y 149
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 39.1 bits (87), Expect = 4e-04
Identities = 19/61 (31%), Positives = 33/61 (54%), Gaps = 1/61 (1%)
Query: 54 ASEDVNHV-IPTAGFNIKSVLSNGFKLNVWDIGGQRKIRPYWRNYFENTDILIYVVDCSD 112
+ +D+ H I T G + ++ L N +D+GGQR R W + FEN L+++V +
Sbjct: 210 SDQDILHCRIKTTGISEETFLLNRHHYRFFDVGGQRSERRKWIHCFENVTALLFLVSLAG 269
Query: 113 H 113
+
Sbjct: 270 Y 270
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 35.5 bits (78), Expect = 0.005
Identities = 16/52 (30%), Positives = 29/52 (55%)
Query: 64 TAGFNIKSVLSNGFKLNVWDIGGQRKIRPYWRNYFENTDILIYVVDCSDHHR 115
T G + S + ++ ++D+GGQR R W FEN + +I+ V +D+ +
Sbjct: 179 TLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYDK 230
>SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 203
Score = 34.7 bits (76), Expect = 0.009
Identities = 23/73 (31%), Positives = 34/73 (46%), Gaps = 5/73 (6%)
Query: 45 GKTTLLKQLASEDVNH-VIPTAG--FNIKSVLSNG--FKLNVWDIGGQRKIRPYWRNYFE 99
GK+ LL + A + I T G F I++ G KL +WD GQ + R +Y+
Sbjct: 20 GKSCLLLRFADDTYTESYISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTITSSYYR 79
Query: 100 NTDILIYVVDCSD 112
+I V D +D
Sbjct: 80 GAHGIIIVYDVTD 92
>SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 201
Score = 32.7 bits (71), Expect = 0.037
Identities = 25/110 (22%), Positives = 47/110 (42%), Gaps = 6/110 (5%)
Query: 43 NAGKTTLLKQLASEDVNHVIP-TAGFNIKS----VLSNGFKLNVWDIGGQRKIRPYWRNY 97
+ GKT+L+ + + ++ T G + S + +L +WD GQ + R +Y
Sbjct: 21 SVGKTSLITRFMYDQFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSY 80
Query: 98 FENTDILIYVVDCSDHHRXXXXXXXXXXXXXDDKLRAVPLLVYANKQDLA 147
++ + I V D ++H+ + + +LV NK DLA
Sbjct: 81 IRDSSVAIIVYDITNHNSFVNTEKWIEDVRAERGDDVIIVLV-GNKTDLA 129
>SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 234
Score = 32.7 bits (71), Expect = 0.037
Identities = 23/77 (29%), Positives = 35/77 (45%), Gaps = 9/77 (11%)
Query: 45 GKTTLLKQLASEDVN-HVIPTAGFNIKS-VLSNGF-------KLNVWDIGGQRKIRPYWR 95
GK+ LL++ + V T G + S ++S G KL +WD GQ K R R
Sbjct: 21 GKSCLLQRFVKNQWDDQVSHTVGIDFASRIISVGMGNQQKRIKLQIWDTAGQEKFRSVAR 80
Query: 96 NYFENTDILIYVVDCSD 112
NY+ + V D ++
Sbjct: 81 NYYRGAAGAVLVYDVTN 97
>SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 200
Score = 31.9 bits (69), Expect = 0.065
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 5/73 (6%)
Query: 45 GKTTLLKQLASEDVN-HVIPTAG--FNIKSVLSNG--FKLNVWDIGGQRKIRPYWRNYFE 99
GK+ LL + + + I T G F I+++ +G KL +WD GQ + R Y+
Sbjct: 21 GKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYR 80
Query: 100 NTDILIYVVDCSD 112
++ + D +D
Sbjct: 81 GAMGILLLYDVTD 93
>SPAC23H4.07c |srp102||signal recognition particle receptor beta
subunit Srp102 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 29.9 bits (64), Expect = 0.26
Identities = 18/45 (40%), Positives = 28/45 (62%), Gaps = 3/45 (6%)
Query: 137 LLVYANKQDLATALPASEIAQHL--GLH-LIRDRTWQIQACVATD 178
LL+ NK DL TA PA +I Q L LH ++ ++ Q+++ V+ D
Sbjct: 143 LLIACNKFDLFTAQPAEKIQQLLKAELHNILEEKNLQLESIVSED 187
>SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr
1|||Manual
Length = 211
Score = 28.3 bits (60), Expect = 0.80
Identities = 11/32 (34%), Positives = 16/32 (50%)
Query: 78 KLNVWDIGGQRKIRPYWRNYFENTDILIYVVD 109
KL +WD GQ + + Y+ N + I V D
Sbjct: 65 KLEIWDTAGQERYKSLAPMYYRNANCAIVVYD 96
>SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 308
Score = 28.3 bits (60), Expect = 0.80
Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 5/36 (13%)
Query: 79 LNVWDIGGQRKIRPYW----RNY-FENTDILIYVVD 109
LN+WD GGQ + R++ F N +LIYV D
Sbjct: 53 LNLWDCGGQEAFMENYLSAQRDHIFRNVQVLIYVFD 88
>SPBC31F10.07 |||cortical component Lsb5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 304
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/27 (40%), Positives = 15/27 (55%)
Query: 89 KIRPYWRNYFENTDILIYVVDCSDHHR 115
+I P N +N I++Y VDC HR
Sbjct: 185 RINPNTENPAKNKQIMVYYVDCKRAHR 211
>SPAC56E4.06c |ggt2||gamma-glutamyltranspeptidase
Ggt2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 611
Score = 27.1 bits (57), Expect = 1.9
Identities = 13/25 (52%), Positives = 13/25 (52%)
Query: 5 GEAKKLVTEWKFHGLLDILKKLRSN 29
GE L WK HGLLD K L N
Sbjct: 177 GELAGLYEAWKSHGLLDWSKLLEPN 201
>SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 205
Score = 26.6 bits (56), Expect = 2.5
Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 7/74 (9%)
Query: 45 GKTTLLKQLA----SEDVNHVIPTAGFNIKSVLSNG--FKLNVWDIGGQRKIRPYWRNYF 98
GKT+++ Q S+D I A F K VL + L +WD GQ + + ++
Sbjct: 20 GKTSIMNQYVNRKFSKDYKATIG-ADFLTKEVLVDDKVVTLQLWDTAGQERFQSLGVAFY 78
Query: 99 ENTDILIYVVDCSD 112
D + V D ++
Sbjct: 79 RGADCCVLVYDVNN 92
>SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 216
Score = 25.8 bits (54), Expect = 4.3
Identities = 18/51 (35%), Positives = 23/51 (45%), Gaps = 5/51 (9%)
Query: 45 GKTTLLKQ-LASEDVNHVIPTAGFNIKSV-LSNGFK---LNVWDIGGQRKI 90
GKTT +K+ L E I T G + + F NVWD GQ K+
Sbjct: 21 GKTTFVKRHLTGEFEKKYIATLGVEVHPLHFHTNFGEICFNVWDTAGQEKL 71
>SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr
1|||Manual
Length = 208
Score = 25.0 bits (52), Expect = 7.5
Identities = 18/74 (24%), Positives = 33/74 (44%), Gaps = 7/74 (9%)
Query: 45 GKTTLLKQLASEDVNHVIPT---AGFNIKSVLSNG--FKLNVWDIGGQRKIRPYWRNYFE 99
GKT L+ Q ++ + A F K V+ + L +WD GQ + + ++
Sbjct: 20 GKTCLMNQFVNQKFSREYKATIGADFLTKDVVVDDKLVTLQLWDTAGQERFQSLGMAFYR 79
Query: 100 NTD--ILIYVVDCS 111
D +++Y V+ S
Sbjct: 80 GADCCVIVYNVNNS 93
>SPBPB2B2.01 |||amino acid permease, unknown 12|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 585
Score = 24.6 bits (51), Expect = 9.9
Identities = 9/37 (24%), Positives = 21/37 (56%)
Query: 72 VLSNGFKLNVWDIGGQRKIRPYWRNYFENTDILIYVV 108
VL+ F ++++ +GG+ ++ NY IL++ +
Sbjct: 494 VLAAEFYVSIFPVGGKPDASAFFENYLSAPVILVFFI 530
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.319 0.136 0.419
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 847,927
Number of Sequences: 5004
Number of extensions: 29720
Number of successful extensions: 86
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 71
Number of HSP's gapped (non-prelim): 19
length of query: 197
length of database: 2,362,478
effective HSP length: 69
effective length of query: 128
effective length of database: 2,017,202
effective search space: 258201856
effective search space used: 258201856
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 51 (24.6 bits)
- SilkBase 1999-2023 -