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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000031-TA|BGIBMGA000031-PA|IPR000873|AMP-dependent
synthetase and ligase, IPR006163|Phosphopantetheine-binding,
IPR002226|Catalase, IPR009081|Acyl carrier protein-like
         (783 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase fam...   152   8e-37
At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c...    78   2e-14
At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ...    75   2e-13
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl...    70   5e-12
At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein ...    68   3e-11
At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase fam...    67   5e-11
At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14) ident...    66   8e-11
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam...    66   8e-11
At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein ...    65   1e-10
At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c...    60   6e-09
At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase fam...    60   6e-09
At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein ...    59   1e-08
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl...    59   1e-08
At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) simil...    57   4e-08
At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl...    55   2e-07
At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase fam...    55   2e-07
At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) simil...    54   3e-07
At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein ...    54   3e-07
At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein ...    54   5e-07
At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil...    50   6e-06
At1g20500.1 68414.m02554 4-coumarate--CoA ligase family / 4-coum...    49   1e-05
At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative / aceta...    46   1e-04
At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl...    45   2e-04
At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase fam...    44   3e-04
At1g21540.1 68414.m02694 AMP-binding protein, putative strong si...    42   0.001
At1g21530.1 68414.m02693 AMP-binding protein, putative strong si...    42   0.002
At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ...    40   0.005
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil...    40   0.006
At5g16340.1 68418.m01910 AMP-binding protein, putative similar t...    39   0.011
At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase fam...    39   0.015
At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase / lon...    39   0.015
At5g27600.1 68418.m03305 AMP-binding protein, putative similar t...    38   0.019
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon...    37   0.045
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si...    37   0.059
At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase, puta...    36   0.078
At3g23790.1 68416.m02990 AMP-binding protein, putative similar t...    36   0.078
At4g14070.1 68417.m02172 AMP-binding protein, putative similar t...    36   0.10 
At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearl...    36   0.14 
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil...    36   0.14 
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil...    36   0.14 
At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase famil...    33   0.96 
At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl...    32   1.3  
At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase / lon...    32   1.7  
At5g46840.1 68418.m05771 RNA recognition motif (RRM)-containing ...    31   2.2  
At5g35430.1 68418.m04213 expressed protein                             31   2.2  
At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase, puta...    31   2.2  
At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative ...    31   2.2  
At5g39870.1 68418.m04836 hypothetical protein                          31   2.9  
At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearl...    31   3.9  
At4g17960.1 68417.m02674 expressed protein                             30   5.1  
At5g58870.1 68418.m07376 FtsH protease, putative contains simila...    29   9.0  
At5g36860.1 68418.m04416 Ulp1 protease family protein contains P...    29   9.0  
At4g08880.1 68417.m01464 Ulp1 protease family protein contains P...    29   9.0  
At3g42730.1 68416.m04462 Ulp1 protease family protein contains P...    29   9.0  
At2g14770.2 68415.m01669 Ulp1 protease family protein similar to...    29   9.0  
At2g14770.1 68415.m01668 Ulp1 protease family protein similar to...    29   9.0  
At1g34740.1 68414.m04319 Ulp1 protease family protein contains P...    29   9.0  
At1g27780.1 68414.m03399 Ulp1 protease family protein similar to...    29   9.0  

>At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase family
           protein similar to iturin A synthetase C [Bacillus
           subtilis] GI:16040972; contains Pfam profile PF00501:
           AMP-binding enzyme
          Length = 1040

 Score =  152 bits (369), Expect = 8e-37
 Identities = 143/482 (29%), Positives = 223/482 (46%), Gaps = 43/482 (8%)

Query: 20  IMALLATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVIYD--DSADPSMFKESGV-P-- 74
           ++++ +  + G A++P++PS+P+ R+  ++  +  SLVI     S +     E  V P  
Sbjct: 16  VISVFSVLRCGEAFLPLDPSWPRERVLSLISSSNISLVIACGLSSVESHWLVERNVCPVL 75

Query: 75  -FASFEELAQEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNR 133
            F+  E+L+ E    S   P   E            ++YTSGSTG PKGV  +   + NR
Sbjct: 76  LFSMDEKLSVETGCSSFVWPCKKER-----QRKFCYLMYTSGSTGKPKGVCGTEQGLLNR 130

Query: 134 LWWQFRTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHGRALLI----LSRE---TTRDP 186
             W    +P    E+   +KT++ F+D + E  G +L   AL+I    L +E   +  D 
Sbjct: 131 FLWMQELYPVV-GEQRFAFKTSVGFIDHIQEFLGAILSSTALVIPPFTLLKENMISIIDF 189

Query: 187 QKLVNIERLVLVPTLLRSILMYLALGPASRPLRR-LKLWVCSGETLSKELASEFFKYFGN 245
            +  +I RL+ VP+++R+IL  L     +  L+  LKL V SGE     L          
Sbjct: 190 LEEYSISRLLAVPSMIRAILPTLQHRGHNNKLQSCLKLVVLSGEPFPVSLWDSLHSLLPE 249

Query: 246 EDGYKLANFYGSTEVMGDVTYYVLEGSDQL---DLYPTIPIGRPLDNCAIYLLDEELSPT 302
                  N YGSTEV GD TY+      +L   +   ++PIG+ + NC + LL +E  P 
Sbjct: 250 T---CFLNLYGSTEVSGDCTYFDCSELPRLLKTEEIGSVPIGKSISNCKVVLLGDEDKPY 306

Query: 303 RDSEPGEVWVAGANLAAGY----VGGGGANRFCDNP---HATQP-EFQRLYRTGDFG-TL 353
                GE+ V+G  L+ GY    +   G  +  +N    H T     Q  YRTGD+G  L
Sbjct: 307 E----GEICVSGLCLSQGYMHSSIESEGYVKLHNNSLCNHLTNDCGSQLYYRTGDYGRQL 362

Query: 354 VKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQVPGVDKCVVLCYGLDRGNPEILGFVTT 413
             G +++ GR D  VK+ G R+ L E+E  L   P + + VVL    +     +  FV  
Sbjct: 363 SSGDLIFIGRRDRTVKLNGKRMALEEIETTLELNPDIAEAVVLLSRDETELASLKAFVVL 422

Query: 414 KPGARTS---AQHIEGKLRSALTHYMMP-QVIEVESIPLLVNGKVDRQGLLKMYENTNNN 469
              + +S      I   +   L   M+P   + VE +PL  +GKVD + L ++   T   
Sbjct: 423 NKESNSSDGIIFSIRNWMGGKLPPVMIPNHFVLVEKLPLTSSGKVDYEALARLKCPTTGA 482

Query: 470 DD 471
            D
Sbjct: 483 QD 484


>At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative /
           4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2
           [gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis
           thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum
          Length = 570

 Score = 78.2 bits (184), Expect = 2e-14
 Identities = 103/444 (23%), Positives = 183/444 (41%), Gaps = 37/444 (8%)

Query: 28  KAGAAYVPMEPSFPQGRITHILKDAEPSLVIYDDSADPSMFKESGVPFASFEELAQEATS 87
           KA AA + +       ++T++  D    +V  DD  D  +   S     SF EL Q    
Sbjct: 142 KASAAKMIITKKCLVDKLTNLKNDGV-LIVCLDDDGDNGVVSSSDDGCVSFTELTQ---- 196

Query: 88  HSADEPMDAETLAPLTSDSIAIVL-YTSGSTGIPKGVRLSY----SAICNRLWWQFRTFP 142
             ADE    E L P  S    + + Y+SG+TG+PKGV +++    ++I  ++  +     
Sbjct: 197 --ADE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLN 251

Query: 143 YSDSEEICVWKTALTFVDSVCEIWGPLLHGRALLILSRETTRDPQKLVNIERLVLVPTLL 202
           ++ ++ I  +            +   +  G ALLI+ R       +L+   ++ +VP   
Sbjct: 252 FTANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAP 311

Query: 203 RSILMYLALGPASR-PLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVM 261
             +L ++      R  L  +++ +    TL KEL       F N         YG TE  
Sbjct: 312 PVVLAFIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNA---IFGQGYGMTE-S 367

Query: 262 GDVTYYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEE--LSPTRDSEPGEVWVAGANLAA 319
           G V   +    +      +   G  + N  + ++D E  +S  R ++ GE+ V G  L  
Sbjct: 368 GTVAKSLAFAKNPFKT-KSGACGTVIRNAEMKVVDTETGISLPR-NKSGEICVRGHQLMK 425

Query: 320 GYVGGGGANRFCDNPHATQPEFQR--LYRTGDFGTL-VKGAVLYAGRTDAQVKIRGHRVD 376
           GY+         ++P AT     +     TGD G +     +    R    +K +G++V 
Sbjct: 426 GYL---------NDPEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVA 476

Query: 377 LLEVERALAQVPGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEGKLRSALTHY- 435
             E+E  L   P +D   V+    +  +   + FV    G++ +   ++  +   + HY 
Sbjct: 477 PAELEALLISHPSIDDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYK 536

Query: 436 MMPQVIEVESIPLLVNGKVDRQGL 459
            +  V  +E IP  V+GK+ R+ L
Sbjct: 537 RIKMVFFIEVIPKAVSGKILRKDL 560


>At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein /
           4-coumaroyl-CoA synthase family protein similar to 4CL2
           [gi:12229665] from Arabidopsis thaliana, 4CL1
           [gi:12229631] from Nicotiana tabacum; contains Pfam
           AMP-binding enzyme domain PF00501
          Length = 562

 Score = 74.9 bits (176), Expect = 2e-13
 Identities = 99/452 (21%), Positives = 185/452 (40%), Gaps = 23/452 (5%)

Query: 24  LATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVIYDDSADPSMFKESGVPFASFEELAQ 83
           LA    G    P  P   +  ++H ++ +EP ++ +  S      + S +P  +    + 
Sbjct: 122 LALMSIGVVVSPANPIGSESEVSHQVEVSEP-VIAFATSQTVKKLQSSSLPLGTVLMDST 180

Query: 84  EATS--HSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSY-SAICNRLWWQFRT 140
           E  S  + +D          +     A +L++SG+TG  KGV L++ + I +      RT
Sbjct: 181 EFLSWLNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRT 240

Query: 141 FPYS-DSEEICVWKTALTFVDSVCEIWGPLLHGRALLILSRETTRDPQKLVNIERLVLVP 199
                + + + ++   L  V     +   +  G  L++L R       K V   ++  +P
Sbjct: 241 LQDPVNYDRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMP 300

Query: 200 TLLRSILMYLALGPASR-PLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGST 258
                I+  +      +  LR L+   C G  L K++A  F + F + D   +   YG T
Sbjct: 301 VSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVD---IVQGYGLT 357

Query: 259 EVMGDVTYYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRD-SEPGEVWVAGANL 317
           E  G        G +++  Y ++  GR  +N    ++D     +    + GE+W+ G  +
Sbjct: 358 ESSGPAASTF--GPEEMVKYGSV--GRISENMEAKIVDPSTGESLPPGKTGELWLRGPVI 413

Query: 318 AAGYVGGGGANRFCDNPHATQPEFQRLYRTGDFGTLVKGAVLY-AGRTDAQVKIRGHRVD 376
             GYVG   A+       A   + +   +TGD         LY   R    +K + ++V 
Sbjct: 414 MKGYVGNEKAS-------AETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVP 466

Query: 377 LLEVERALAQVPGVDKCVVLCYGLDRGNPEILGFVTTKPGAR-TSAQHIEGKLRSALTHY 435
            +E+E+ L   P V    V+ +  +      + F+  KPG+    AQ I+   +    + 
Sbjct: 467 PVELEQILHSNPDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYK 526

Query: 436 MMPQVIEVESIPLLVNGKVDRQGLLKMYENTN 467
            + +V  + +IP    GK+ R+ L K+  + N
Sbjct: 527 KVRRVAFINAIPKNPAGKILRRELTKIAVDGN 558


>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
           4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
           4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
          Length = 561

 Score = 70.1 bits (164), Expect = 5e-12
 Identities = 91/394 (23%), Positives = 164/394 (41%), Gaps = 32/394 (8%)

Query: 78  FEELAQEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRLWWQ 137
           F EL Q  T   A E +D+  ++P   D +  + Y+SG+TG+PKGV L++  +   +  Q
Sbjct: 180 FTELTQSTTE--ASEVIDSVEISP---DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQ 234

Query: 138 F-----RTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHGRALLILSRETTRDPQKLVNI 192
                   + +SD   +CV      +  +   + G L  G A+LI+ +       +L+  
Sbjct: 235 VDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCG-LRVGAAILIMPKFEINLLLELIQR 293

Query: 193 ERLVLVPTLLRSILMYLALGPASR--PLRRLKLWVCSGETLSKELASEFFKYFGNEDGYK 250
            ++ + P ++  I++ +A    +    L  +++       L KEL       F N    K
Sbjct: 294 CKVTVAP-MVPPIVLAIAKSSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNA---K 349

Query: 251 LANFYGSTEVMGDVTYYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRD-SEPGE 309
           L   YG TE  G V    L  + +     +   G  + N  + ++D +   +   ++PGE
Sbjct: 350 LGQGYGMTEA-GPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGE 408

Query: 310 VWVAGANLAAGYVGGGGANRFCDNPHATQPEFQR--LYRTGDFGTLVKGAVLY-AGRTDA 366
           + + G  +  GY+         +NP AT     +     TGD G +     L+   R   
Sbjct: 409 ICIRGHQIMKGYL---------NNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKE 459

Query: 367 QVKIRGHRVDLLEVERALAQVPGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEG 426
            +K +G +V   E+E  L   P +    V+    +      + FV     +  S   ++ 
Sbjct: 460 LIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVAFVVKSKDSELSEDDVKQ 519

Query: 427 KLRSALTHY-MMPQVIEVESIPLLVNGKVDRQGL 459
            +   +  Y  + +V   ESIP   +GK+ R+ L
Sbjct: 520 FVSKQVVFYKRINKVFFTESIPKAPSGKILRKDL 553


>At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein /
           4-coumaroyl-CoA synthase family protein similar to
           SP|P14912 and SP|P14913 from Petroselinum crispum;
           contains Pfam AMP-binding enzyme domain PF00501
          Length = 546

 Score = 67.7 bits (158), Expect = 3e-11
 Identities = 86/381 (22%), Positives = 156/381 (40%), Gaps = 27/381 (7%)

Query: 92  EPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRLWWQFRTFPYSDSEEICV 151
           EP        +  D  A +LY+SG+TG+ KGV  S+  +   +      F   D E+  +
Sbjct: 176 EPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFI 235

Query: 152 WKTALTFVDSVCEI-WGPLLHGRALLILSRETTRDPQKLVN---IERLVLVPTLLRSILM 207
               +  +  +     G L +G  +++LS+    +    +       L LVP +L +++ 
Sbjct: 236 CTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVN 295

Query: 208 YLALGPASRPLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMG-DVTY 266
                 A   L  +   +C G  LSKE+   F + +      K+   YG TE  G   + 
Sbjct: 296 GADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPT---VKILQGYGLTESTGIGAST 352

Query: 267 YVLEGSDQLDLYPTIPI---GRPLDNCAIYLLDEELSPTRDSEPGEVWVAGANLAAGYVG 323
             +E S +      +     GR +D     +  + L P    + GE+W+ G ++  GY  
Sbjct: 353 DTVEESRRYGTAGKLSASMEGRIVDP----VTGQILGP---KQTGELWLKGPSIMKGYFS 405

Query: 324 GGGANRFCDNPHATQPEFQRLYRTGDFGTLVK-GAVLYAGRTDAQVKIRGHRVDLLEVER 382
              A        ++  + +   RTGD   + + G +    R    +K +G++V   E+E 
Sbjct: 406 NEEAT-------SSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEA 458

Query: 383 ALAQVPGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHI-EGKLRSALTHYMMPQVI 441
            L   P +    V+ +         + +V  K G+  S + I E   +    +  + +V 
Sbjct: 459 LLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVA 518

Query: 442 EVESIPLLVNGKVDRQGLLKM 462
            V SIP   +GK+ R+ L+K+
Sbjct: 519 FVSSIPKNPSGKILRKDLIKI 539


>At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase family
           protein similar to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501; identical to cDNA adenosine monophosphate
           binding protein 2 AMPBP2 (AMPBP2) GI:20799712
          Length = 603

 Score = 66.9 bits (156), Expect = 5e-11
 Identities = 96/416 (23%), Positives = 158/416 (37%), Gaps = 22/416 (5%)

Query: 56  LVIYDDSADPSMFKESGVPFASFEELAQEATSHSADEPMDAETLAPLTSDSIAIVLYTSG 115
           ++I   + D    ++S   FAS      E  +       + E + P        + YTSG
Sbjct: 192 VLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIKPRCEWDPISINYTSG 251

Query: 116 STGIPKGVRLSY-SAICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHGRA 174
           +T  PKGV  S+  A  N L   F          + +W   +   +  C +WG    G  
Sbjct: 252 TTSRPKGVVYSHRGAYLNSLATVF--LHQMSVYPVYLWTVPMFHCNGWCLVWGVAAQGGT 309

Query: 175 LLILSRETTRDPQKLVNIERLVLV---PTLLRSILMYLALGPASRPLRRLKLWVCSGETL 231
            + L + + +   K + + ++  +   PT+L  I+ Y        P  R+++       L
Sbjct: 310 NICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLP-HRVEIMTGGSPPL 368

Query: 232 SKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGS-DQLDLYPTIPIG--RPLD 288
            + LA         E G+ +++ YG TE  G  T+ V +   D L L     +   + + 
Sbjct: 369 PQILAKM------EELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKARQGVQ 422

Query: 289 NCAIYLLDEELSPTRDSEPGEVWVAGANLAAGYVGGGGANRFCDNPHATQPEFQR-LYRT 347
           +  +  LD +   T ++ P +    G  +  G     G   +  +  AT+  F+   + +
Sbjct: 423 HLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSG---YFKDIEATRKAFEGDWFHS 479

Query: 348 GDFGTLVK-GAVLYAGRTDAQVKIRGHRVDLLEVERALAQVPGVDKCVVLCYGLDRGNPE 406
           GD       G +    R    +   G  +  +EVER L     V +  V+          
Sbjct: 480 GDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWGQT 539

Query: 407 ILGFVTTKPGART-SAQHIEGKLRSALTHYMMPQVIEVESIPLLVNGKVDRQGLLK 461
             GFV  K G  T   + I G  R  L HYM P+ I    IP    GKV +  L K
Sbjct: 540 PCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLRK 595


>At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14)
           identical to acyl-activating enzyme 14 [Arabidopsis
           thaliana]; similar to SP|Q42524 4-coumarate--CoA ligase
           1 (EC 6.2.1.12) (4-coumaroyl-CoA synthase 1)
           {Arabidopsis thaliana}; contains Pfam profile PF00501:
           AMP-binding enzyme; identical to cDNA acyl-activating
           enzyme 14 (At1g30520) GI:29893263
          Length = 560

 Score = 66.1 bits (154), Expect = 8e-11
 Identities = 75/338 (22%), Positives = 134/338 (39%), Gaps = 32/338 (9%)

Query: 80  ELAQEATSHSADEPMDAETLAPL------TSDSIAIVLYTSGSTGIPKGVRLSYSAICNR 133
           + A E       E +   TL P        SD   ++ +TSG+TG PKGV +S+ A   +
Sbjct: 141 DFANELNQFLTTEMLKQRTLVPSLATYAWASDDAVVICFTSGTTGRPKGVTISHLAFITQ 200

Query: 134 LWWQFRTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHGRALLILSR---ETTRDPQKLV 190
              +     Y + +++ +  + L  +  +      L+ G   ++L +   +T     +  
Sbjct: 201 SLAKIAIAGYGE-DDVYLHTSPLVHIGGLSSAMAMLMVGACHVLLPKFDAKTALQVMEQN 259

Query: 191 NIERLVLVPTLLRSILMYLALGPASRPLRRLKLWVCSGETLSKELASEFFKYFGNEDGYK 250
           +I   + VP ++  ++            R ++  +  G +LS EL  E    F      +
Sbjct: 260 HITCFITVPAMMADLIRVNRTTKNGAENRGVRKILNGGGSLSSELLKEAVNIF---PCAR 316

Query: 251 LANFYGSTEVMGDVTYYVLEGSDQLDLYPTIP----------IGRPLDNCAIYL-LDEEL 299
           + + YG TE    +T+  L    Q     T P          +G+P  +  + + LDE+ 
Sbjct: 317 ILSAYGMTEACSSLTFMTLHDPTQESFKVTYPLLNQPKQGTCVGKPAPHIELMVKLDED- 375

Query: 300 SPTRDSEPGEVWVAGANLAAGYVGGGGANRFCDNPHATQPEFQRLYRTGDFGTLVK-GAV 358
                S  G++   G +    Y G   A    +   +   E      TGD G   + G +
Sbjct: 376 ----SSRVGKILTRGPHTMLRYWGHQVAQENVETSESRSNE--AWLDTGDIGAFDEFGNL 429

Query: 359 LYAGRTDAQVKIRGHRVDLLEVERALAQVPGVDKCVVL 396
              GR++ ++K  G  V   EVE  L + PG+   VV+
Sbjct: 430 WLIGRSNGRIKTGGENVYPEEVEAVLVEHPGIVSAVVI 467


>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
           protein similar to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501; identical to adenosine monophosphate binding
           protein 1 AMPBP1 (AMPBP1) GI:20799710
          Length = 556

 Score = 66.1 bits (154), Expect = 8e-11
 Identities = 91/370 (24%), Positives = 137/370 (37%), Gaps = 19/370 (5%)

Query: 95  DAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSY-SAICNRLWWQFRTFPYSDSEEICVWK 153
           D E + P        V YTSG+T  PKGV  S+  A  N L        +S    +  W 
Sbjct: 177 DFEVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYL--WT 234

Query: 154 TALTFVDSVCEIWGPLLHGRALLILSRETTR---DPQKLVNIERLVLVPTLLRSILMYLA 210
             +   +  C +WG    G   + L   T +   D      +  +   PT+L  I+   A
Sbjct: 235 NPMFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIIN--A 292

Query: 211 LGPASRPLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLE 270
                +PL   K+   +G   +   A   FK    E G+ + + YG TE  G  T  +  
Sbjct: 293 PESEQKPLPG-KVSFITGA--APPPAHVIFKM--EELGFSMFHSYGLTETYGPGT--ICT 345

Query: 271 GSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRDSEPGEVWVAGANLAAGYVGGGGA--N 328
              + D  P     +      +  L  E    +D        A        V  G    N
Sbjct: 346 WKPEWDSLPREEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMN 405

Query: 329 RFCDNPHATQPEFQR-LYRTGDFGTL-VKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQ 386
            +  NP AT+  F+   + +GD G     G +    R+   +   G  +  +EVE  L  
Sbjct: 406 GYLKNPEATKEAFKGGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFT 465

Query: 387 VPGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEGKLRSALTHYMMPQVIEVESI 446
            P V +  V+    +        FV  K G++ SA+ +    R  L HYM P+ I  E +
Sbjct: 466 HPCVLEAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDL 525

Query: 447 PLLVNGKVDR 456
           P    GKV +
Sbjct: 526 PKTSTGKVQK 535


>At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein /
           4-coumaroyl-CoA synthase family protein similar to 4CL
           from Pinus taeda, gi:515503, gi:1143308; contains Pfam
           AMP-binding enzyme domain PF00501
          Length = 566

 Score = 65.3 bits (152), Expect = 1e-10
 Identities = 105/477 (22%), Positives = 185/477 (38%), Gaps = 43/477 (9%)

Query: 12  PHFFYFSTIMALLATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVIYDDSADPSMFKES 71
           P+  YF   M  L+    GA    M PS   G I   + +    L  +  + +       
Sbjct: 106 PNSVYFP--MIFLSLISLGAIVTTMNPSSSLGEIKKQVSECSVGLA-FTSTENVEKLSSL 162

Query: 72  GVPFASFEELAQEATSHSADEPM------DAETLAP---LTSDSIAIVLYTSGSTGIPKG 122
           GV   S  E + +  S   + P       ++    P   +  D +A ++Y+SG+TG  KG
Sbjct: 163 GVSVISVSE-SYDFDSIRIENPKFYSIMKESFGFVPKPLIKQDDVAAIMYSSGTTGASKG 221

Query: 123 VRLSYSAI--CNRLWWQFRT--FPYSDSEEICVWKTALTFVDSV-CEIWGPLLHGRALLI 177
           V L++  +     L+ +F    + Y  S  + +    L  +  +   + G L  G  +++
Sbjct: 222 VLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVV 281

Query: 178 LSRETTRDPQKLV---NIERLVLVPTLLRSILMYLALGPASRPLRRLKLWVCSGETLSKE 234
           + R    D   ++    I    +VP +L + L   A G      + LK        LS++
Sbjct: 282 MKRFDASDVVNVIERFKITHFPVVPPMLMA-LTKKAKGVCGEVFKSLKQVSSGAAPLSRK 340

Query: 235 LASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLDLYPTIPIGRPLDNCAIYL 294
              +F +   + D   L   YG TE     T      S++L  Y ++ +  P  N    +
Sbjct: 341 FIEDFLQTLPHVD---LIQGYGMTESTAVGTRGF--NSEKLSRYSSVGLLAP--NMQAKV 393

Query: 295 LD-EELSPTRDSEPGEVWVAGANLAAGYVGGGGANRFCDNPHATQPEF--QRLYRTGDFG 351
           +D    S       GE+W+ G  +  GY+         +NP ATQ         RTGD  
Sbjct: 394 VDWSSGSFLPPGNRGELWIQGPGVMKGYL---------NNPKATQMSIVEDSWLRTGDIA 444

Query: 352 TLVKGAVLY-AGRTDAQVKIRGHRVDLLEVERALAQVPGVDKCVVLCYGLDRGNPEILGF 410
              +   L+   R    +K +G ++   ++E  L   P +    V     +      + F
Sbjct: 445 YFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAF 504

Query: 411 VTTKPGARTSAQHIEGKLRSALTHY-MMPQVIEVESIPLLVNGKVDRQGLLKMYENT 466
           V  +     S + +   + S +  Y  + +V+ V SIP    GK+ R+ L ++  N+
Sbjct: 505 VVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILRKELKRILTNS 561


>At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative /
           4-coumaroyl-CoA synthase, putative similar to 4CL2
           [gi:12229665] from Arabidopsis thaliana, 4CL1
           [gi:12229631] from Nicotiana tabacum; contains Pfam
           AMP-binding enzyme domain PF00501; acyl-activating
           enzyme superfamily; identical to cDNA 4-coumarate-CoA
           ligase-like protein (At4g05160) GI:29893226
          Length = 544

 Score = 60.1 bits (139), Expect = 6e-09
 Identities = 103/464 (22%), Positives = 177/464 (38%), Gaps = 42/464 (9%)

Query: 21  MALLATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVI-----YDDSAD---PSMFKESG 72
           +  LA    G  +    P +    ++  +KD+ P ++I     +D       P +   S 
Sbjct: 94  LCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSK 153

Query: 73  ----VPFASFEELAQEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSY- 127
               +P  S  ++          EP+       +     A +LY+SG+TG  KGV L++ 
Sbjct: 154 DTVEIPPGSNSKILSFDNVMELSEPVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHG 213

Query: 128 SAICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVCEI-WGPLLHGRALLILSRETTRDP 186
           + I   L          +   + +    +  V  +  I +  L  G AL+ ++R      
Sbjct: 214 NFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNALVSMARFELE-- 271

Query: 187 QKLVNIERLVLVPTLLRSILMYLALGPASR----PLRRLKLWVCSGETLSKELASEFFKY 242
             L NIE+   V  L     ++LAL   S      L  LK        L K+L  E  + 
Sbjct: 272 LVLKNIEKF-RVTHLWVVPPVFLALSKQSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRN 330

Query: 243 FGNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPT 302
             N     L   YG TE  G V+        +L    +   G         ++  E   +
Sbjct: 331 IPN---VLLMQGYGMTETCGIVSVE----DPRLGKRNSGSAGMLAPGVEAQIVSVETGKS 383

Query: 303 RD-SEPGEVWVAGANLAAGYVGGGGANRFCDNPHATQPEFQR--LYRTGDFGTLVKGAVL 359
           +  ++ GE+WV G N+  GY+         +NP AT+    +     TGD G   +   L
Sbjct: 384 QPPNQQGEIWVRGPNMMKGYL---------NNPQATKETIDKKSWVHTGDLGYFNEDGNL 434

Query: 360 Y-AGRTDAQVKIRGHRVDLLEVERALAQVPGVDKCVVLCYGLDRGNPEILGFVTTKPGAR 418
           Y   R    +K +G +V   E+E  L   P +   VV+ +  +      + FV   P + 
Sbjct: 435 YVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSS 494

Query: 419 TSAQHIEGKLRSALTHY-MMPQVIEVESIPLLVNGKVDRQGLLK 461
            + Q I+  +   +  Y  + +V  +  +P    GK+ R+ L++
Sbjct: 495 ITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQ 538


>At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase family
           protein similar to peroxisomal-coenzyme A synthetase
           (FAT2) [gi:586339] from Saccharomyces cerevisiae;
           contains Pfam AMP-binding enzyme domain PF00501;
           identical to cDNA; identical to cDNA adenosine
           monophosphate binding protein 3 AMPBP3
           (AMPBP3)GI:20799714
          Length = 514

 Score = 60.1 bits (139), Expect = 6e-09
 Identities = 94/455 (20%), Positives = 180/455 (39%), Gaps = 37/455 (8%)

Query: 20  IMALLATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVIYDDSAD-PSMFKESGVPFASF 78
           ++  LA  +A A   P+  ++        L D++  L++     + P+    S +  +  
Sbjct: 70  VIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEAASKLKISHV 129

Query: 79  EELAQEATSH------SADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICN 132
                +A S        +D  +D+ T      D  A+ L+TSG+T  PKGV L+   + +
Sbjct: 130 TATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGVPLTQLNLAS 189

Query: 133 RLWWQFRTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHGRALLI-----LSRETTRDPQ 187
            +      +  ++S+   +          +  +   L  G A+ +      S  T     
Sbjct: 190 SVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRFSATTFWPDM 249

Query: 188 KLVNIERLVLVPTLLRSILMYLALGPASRPLRRLKLWVCSGETLSKELASEFFKYFGNED 247
           K  N      VPT+ + IL   A  P +   +   +  CS  +L+  + S   + FG   
Sbjct: 250 KKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSA-SLAPVILSRLEEAFGAP- 307

Query: 248 GYKLANFYGSTEVMGDVTYYVLEGSDQLDLYPTIP--IGRPLDNCAIYLLDEELSPTRDS 305
              +   Y  TE       +++  +   +  P  P  +G+P+    + +L+E+      +
Sbjct: 308 ---VLEAYAMTEAT-----HLMSSNPLPEEGPHKPGSVGKPVGQ-EMAILNEKGEIQEPN 358

Query: 306 EPGEVWVAGANLAAGYVGGGGANRFCDNPHATQPEFQ-RLYRTGDFGTLVKGAVLY-AGR 363
             GEV + G N+  GY          +NP A +  F+   + TGD G       L+  GR
Sbjct: 359 NKGEVCIRGPNVTKGYK---------NNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGR 409

Query: 364 TDAQVKIRGHRVDLLEVERALAQVPGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQH 423
               +   G ++  +EV+  L   P V + V      ++   EI   V  + G   + + 
Sbjct: 410 IKELINRGGEKISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEED 469

Query: 424 IEGKLRSALTHYMMP-QVIEVESIPLLVNGKVDRQ 457
           I+   +  L  + +P +V   +++P   +GK+ R+
Sbjct: 470 IKAFCKKNLAAFKVPKRVFITDNLPKTASGKIQRR 504


>At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein /
           4-coumaroyl-CoA synthase family protein similar to 4CL2,
           Arabidopsis thaliana [gi:12229665], 4CL1, Nicotiana
           tabacum [gi:12229631]; contains Pfam AMP-binding enzyme
           domain PF00501
          Length = 550

 Score = 59.3 bits (137), Expect = 1e-08
 Identities = 83/379 (21%), Positives = 152/379 (40%), Gaps = 29/379 (7%)

Query: 92  EPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRLWWQFRTFPYSDSEEICV 151
           EP        +  D  A++LY+SG+TG  KGV  S+  +   +  ++   P+   ++  +
Sbjct: 186 EPSGQAVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIAHV-ARYIAEPFEQPQQTFI 244

Query: 152 WKTALTFVDSVCE-IWGPLLHGRALLILSRETTRDPQKLVNIER---LVLVPTLLRSILM 207
               L     +   +   L  G  ++IL R    +    V   R   L+LVP +L +++ 
Sbjct: 245 CTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATTLILVPPVLVTMIN 304

Query: 208 YLALGPASRPLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYY 267
                     +  L+   C G  LSKE+   F K +   D Y+    Y  TE  G     
Sbjct: 305 KADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQ---GYALTESNG--AGA 359

Query: 268 VLEGSDQLDLYPTIPIGRPLDNCAI--YLLDEELSPTRD-SEPGEVWVAGANLAAGYVGG 324
            +E  ++   Y  +     L +C +   ++D         ++ GE+W+ G ++A GY   
Sbjct: 360 SIESVEESRRYGAV----GLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKGY--- 412

Query: 325 GGANRFCDNPHATQPEFQRLYRTGDFGTLVKGAVLY-AGRTDAQVKIRGHRVDLLEVERA 383
                F +       E     +TGD   +     L+   R    +K +G++V   E+E  
Sbjct: 413 -----FRNEEEIITSE--GWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEAL 465

Query: 384 LAQVPGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEGKLRSALTHY-MMPQVIE 442
           L   P +    V+ +         + +V  KP +    + +   +   +  Y  + +V  
Sbjct: 466 LLNHPDILDAAVIPFPDKEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAF 525

Query: 443 VESIPLLVNGKVDRQGLLK 461
           ++SIP   +GK  R+ L+K
Sbjct: 526 IDSIPKTPSGKTLRKDLIK 544


>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
           4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
           4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
          Length = 490

 Score = 58.8 bits (136), Expect = 1e-08
 Identities = 78/330 (23%), Positives = 139/330 (42%), Gaps = 31/330 (9%)

Query: 78  FEELAQEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRLWWQ 137
           F EL Q  T   A E +D+  ++P   D +  + Y+SG+TG+PKGV L++  +   +  Q
Sbjct: 180 FTELTQSTTE--ASEVIDSVEISP---DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQ 234

Query: 138 F-----RTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHGRALLILSRETTRDPQKLVNI 192
                   + +SD   +CV      +  +   + G L  G A+LI+ +       +L+  
Sbjct: 235 VDGENPNLYFHSDDVILCVLPMFHIYALNSIMLCG-LRVGAAILIMPKFEINLLLELIQR 293

Query: 193 ERLVLVPTLLRSILMYLALGPASR--PLRRLKLWVCSGETLSKELASEFFKYFGNEDGYK 250
            ++ + P ++  I++ +A    +    L  +++       L KEL       F N    K
Sbjct: 294 CKVTVAP-MVPPIVLAIAKSSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNA---K 349

Query: 251 LANFYGSTEVMGDVTYYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRD-SEPGE 309
           L   YG TE  G V    L  + +     +   G  + N  + ++D +   +   ++PGE
Sbjct: 350 LGQGYGMTEA-GPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGE 408

Query: 310 VWVAGANLAAGYVGGGGANRFCDNPHATQPEFQR--LYRTGDFGTLVKGAVLY-AGRTDA 366
           + + G  +  GY+         +NP AT     +     TGD G +     L+   R   
Sbjct: 409 ICIRGHQIMKGYL---------NNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKE 459

Query: 367 QVKIRGHRVDLLEVERALAQVPGVDKCVVL 396
            +K +G +V   E+E  L   P +    V+
Sbjct: 460 LIKYKGFQVAPAELEALLIGHPDITDVAVV 489


>At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) similar
           to AMP-binding protein GI:1903034 from [Brassica napus];
           contains Pfam AMP-binding domain PF00501; identical to
           cDNA acyl-activating enzyme 11 (At1g66120) GI:29893230,
           acyl-activating enzyme 11 [Arabidopsis thaliana]
           GI:29893231
          Length = 572

 Score = 57.2 bits (132), Expect = 4e-08
 Identities = 87/370 (23%), Positives = 147/370 (39%), Gaps = 53/370 (14%)

Query: 112 YTSGSTGIPKGVRLSY-----SAICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVCEIW 166
           YTSG+T  PKGV +S+     SA+ + + W+   FP      + +W   +   +     W
Sbjct: 193 YTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFP------VYLWTLPMFHCNGWTHTW 246

Query: 167 GPLLHGRALLILSRETTRDPQK---LVNIERLVLVPTLLRSIL--MYLALGPASRPLRRL 221
                G   + +   T  +  K   L  +  +  VPT+ R +L        P S P++ L
Sbjct: 247 SVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKSSPVQVL 306

Query: 222 KLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLDLYP-- 279
                    L K++          + G+ + + YG TE  G V +   E  D+ +  P  
Sbjct: 307 TGGSSPPAVLIKKV---------EQLGFHVMHGYGLTEATGPVLF--CEWQDEWNKLPEH 355

Query: 280 ---TIPIGRPLDNCAIYLLDEELSPTRDSEP------GEVWVAGANLAAGYVGGGGANRF 330
               +   + + N  +  +D + + T +S P      GE+ + G++L  GY+        
Sbjct: 356 QQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYL-------- 407

Query: 331 CDNPHATQPEFQRLY-RTGDFGTL-VKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQVP 388
             NP AT   F+  +  TGD G +   G V    R+   +   G  +  +EVE+ L    
Sbjct: 408 -KNPKATSEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQ 466

Query: 389 GVDKCVVLCYGLDRGNPEILGFVTTKPGAR---TSAQHIEGKLRSALTHYMMP-QVIEVE 444
            V +  V+             FV  K G     TS   +    R  + H+M P +V+  +
Sbjct: 467 EVLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQ 526

Query: 445 SIPLLVNGKV 454
            +P   NGK+
Sbjct: 527 ELPKNSNGKI 536


>At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 /
           4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725
           4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
           (4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana}
          Length = 556

 Score = 55.2 bits (127), Expect = 2e-07
 Identities = 84/383 (21%), Positives = 159/383 (41%), Gaps = 29/383 (7%)

Query: 90  ADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRLWWQF---RTFPYSDS 146
           ++EP        ++ + +  + ++SG+TG+PKGV L++  +   +  Q        Y + 
Sbjct: 180 SEEPRVDSIPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNR 239

Query: 147 EEICVWKTALTFVDSVCEIW-GPLLHGRALLILSR-ETTRDPQKLVNIERL-VLVPTLLR 203
           +++ +    +  + ++  I    L  G  +LI+ + E T     L  I+R  V V  ++ 
Sbjct: 240 DDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEIT---LLLEQIQRCKVTVAMVVP 296

Query: 204 SILMYLALGPASR--PLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVM 261
            I++ +A  P +    L  +++       L KEL       F N    KL   YG TE  
Sbjct: 297 PIVLAIAKSPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNA---KLGQGYGMTEA- 352

Query: 262 GDVTYYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRD-SEPGEVWVAGANLAAG 320
           G V    L  + +     +   G  + N  + +LD +   +   ++PGE+ + G  +  G
Sbjct: 353 GPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKG 412

Query: 321 YVGGGGANRFCDNPHATQPEFQR--LYRTGDFGTLVKGAVLY-AGRTDAQVKIRGHRVDL 377
           Y+         ++P AT     +     TGD G +     L+   R    +K +G +V  
Sbjct: 413 YL---------NDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAP 463

Query: 378 LEVERALAQVPGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEGKLRSALTHY-M 436
            E+E  L   P ++   V+    +      + FV     +  S   I+  +   +  Y  
Sbjct: 464 AELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKR 523

Query: 437 MPQVIEVESIPLLVNGKVDRQGL 459
           + +V   +SIP   +GK+ R+ L
Sbjct: 524 INKVFFTDSIPKAPSGKILRKDL 546


>At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase family
           protein similar to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501
          Length = 580

 Score = 54.8 bits (126), Expect = 2e-07
 Identities = 96/416 (23%), Positives = 161/416 (38%), Gaps = 63/416 (15%)

Query: 76  ASFEELAQEATSHSADE-PMDAETLAPLTSDSIAIVL-YTSGSTGIPKGVRLSY--SAIC 131
           ASFEEL  E      +  P     +  +  +   I L YTSG+T  PKGV +S+  + +C
Sbjct: 155 ASFEELDYECLIQRGEPTPSMVARMFRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLC 214

Query: 132 NR---LWWQFRTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHGRALLILSRETTRDPQK 188
                + W+  T P      + +W   +   +     WG    G   + +   T  +  K
Sbjct: 215 TLSAIIGWEMGTCP------VYLWTLPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYK 268

Query: 189 LV---NIERLVLVPTLLRSILM--YLALGPASRPLRRLKLWVCSGETLSKELASEFFKYF 243
            +   N+  +  VPT+   +L    L L P S P+  L      G      L  +  +  
Sbjct: 269 NIEMHNVTHMCCVPTVFNILLKGNSLDLSPRSGPVHVL----TGGSPPPAALVKKVQRL- 323

Query: 244 GNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLDLYP-----TIPIGRPLDNCAIYLLDEE 298
               G+++ + YG TE  G + +   E  D+ +  P      +   + +    +  +D +
Sbjct: 324 ----GFQVMHAYGQTEATGPILF--CEWQDEWNRLPENQQMELKARQGISILGLADVDVK 377

Query: 299 LSPTRDSEP------GEVWVAGANLAAGYVGGGGANRFCDNPHATQPEFQRLY-RTGDFG 351
              T+ S P      GE+ + G+++  GY+          NP AT   F+  +  TGD G
Sbjct: 378 NKETQKSAPRDGKTMGEILIKGSSIMKGYL---------KNPKATFEAFKHGWLNTGDVG 428

Query: 352 TL-VKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQVPGVDKCVVLCYGLDRGNPEILGF 410
            +   G V    R+   +   G  +  +EVE  L + P V +  V+             F
Sbjct: 429 VIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCAF 488

Query: 411 VTTKPGARTSAQHIEGKL-----------RSALTHYMMP-QVIEVESIPLLVNGKV 454
           V  +    T  +    K            R  L H+M P +V+ +E +P   NGK+
Sbjct: 489 VVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNGNGKI 544


>At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) similar
           to malonyl CoA synthetase GB:AAF28840 from
           [Bradyrhizobium japonicum]; contains Pfam AMP-binding
           enzyme domain PF00501; identical to cDNA acyl-activating
           enzyme 13 (At3g16170) GI:29893232, acyl-activating
           enzyme 13 [Arabidopsis thaliana] GI:29893233
          Length = 544

 Score = 54.4 bits (125), Expect = 3e-07
 Identities = 31/133 (23%), Positives = 66/133 (49%), Gaps = 5/133 (3%)

Query: 20  IMALLATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVIYDDSADPSM---FKESGVPFA 76
           +  +L TW +G   VP+  S+P+  + H++ D++ SL++  +    +M     +SG  F 
Sbjct: 80  VAGVLGTWFSGGVAVPLALSYPEAELLHVMNDSDISLLLSTEDHSETMKTIAAKSGARFH 139

Query: 77  SFEELAQEATSHSADEPMDAETLAPLTS--DSIAIVLYTSGSTGIPKGVRLSYSAICNRL 134
               +    +   A      ++        D  A+++YTSG+TG PKGV  ++++I +++
Sbjct: 140 LIPPVVNSTSETVACNQFQDDSFEAEGKFLDDPALIVYTSGTTGKPKGVVHTHNSINSQV 199

Query: 135 WWQFRTFPYSDSE 147
                 + Y+ ++
Sbjct: 200 RMLTEAWEYTSAD 212



 Score = 43.6 bits (98), Expect = 5e-04
 Identities = 37/147 (25%), Positives = 68/147 (46%), Gaps = 15/147 (10%)

Query: 334 PHATQPEFQR--LYRTGDFGTLVK-GAVLYAGRTDAQV-KIRGHRVDLLEVERALAQVPG 389
           P  T+  F     ++TGD G + + G  +  GR  A + K+ G+++  LE+E  L + P 
Sbjct: 396 PEVTKESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPT 455

Query: 390 VDKCVVLCYGLDRGNPEILGFVTTKPGAR----------TSAQHIEGKLRSALTHYMMP- 438
           V +C VL    +     +   +  +  A+           + + + G  +  L  Y +P 
Sbjct: 456 VAECCVLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPT 515

Query: 439 QVIEVESIPLLVNGKVDRQGLLKMYEN 465
           +++  ES+P    GKV+++ L K  EN
Sbjct: 516 RLLIWESLPRNAMGKVNKKELKKSLEN 542


>At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein /
           4-coumaroyl-CoA synthase family protein similar to
           SP|P14912 and SP|P14913 from Petroselinum crispum;
           contains Pfam AMP-binding enzyme domain PF00501
          Length = 473

 Score = 54.4 bits (125), Expect = 3e-07
 Identities = 70/314 (22%), Positives = 126/314 (40%), Gaps = 26/314 (8%)

Query: 92  EPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRLWWQFRTFPYSDSEEICV 151
           EP        +  D  A +LY+SG+TG+ KGV  S+  +   +      F   D E+  +
Sbjct: 176 EPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFI 235

Query: 152 WKTALTFVDSVCEI-WGPLLHGRALLILSRETTRDPQKLVN---IERLVLVPTLLRSILM 207
               +  +  +     G L +G  +++LS+    +    +       L LVP +L +++ 
Sbjct: 236 CTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVN 295

Query: 208 YLALGPASRPLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMG-DVTY 266
                 A   L  +   +C G  LSKE+   F + +      K+   YG TE  G   + 
Sbjct: 296 GADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPT---VKILQGYGLTESTGIGAST 352

Query: 267 YVLEGSDQLDLYPTIPI---GRPLDNCAIYLLDEELSPTRDSEPGEVWVAGANLAAGYVG 323
             +E S +      +     GR +D     +  + L P    + GE+W+ G ++  GY  
Sbjct: 353 DTVEESRRYGTAGKLSASMEGRIVDP----VTGQILGP---KQTGELWLKGPSIMKGYFS 405

Query: 324 GGGANRFCDNPHATQPEFQRLYRTGDFGTLVK-GAVLYAGRTDAQVKIRGHRVDLLEVER 382
              A        ++  + +   RTGD   + + G +    R    +K +G++V   E+E 
Sbjct: 406 NEEAT-------SSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEA 458

Query: 383 ALAQVPGVDKCVVL 396
            L   P +    V+
Sbjct: 459 LLLTHPEITDAAVI 472


>At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein /
           4-coumaroyl-CoA synthase family protein similar to
           SP|Q9S725 from Arabidopsis thaliana and SP|P17814 from
           Oryza sativa; contains Pfam AMP-binding enzyme domain
           PF00501
          Length = 565

 Score = 53.6 bits (123), Expect = 5e-07
 Identities = 83/377 (22%), Positives = 147/377 (38%), Gaps = 22/377 (5%)

Query: 92  EPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRLWWQFRTFPYSDSEEICV 151
           EP ++     +  D  A +LY+SG+TG  KGV LS+  +   L   +R     +   IC 
Sbjct: 200 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLI-ALVQAYRARFGLEQRTICT 258

Query: 152 WKTALTFVDSVCEIWGPLLHGRALLILSRETTRDPQKLVNIER---LVLVPTLLRSILMY 208
                 F        G +  G  +++L +         V   R   L LVP ++ +++  
Sbjct: 259 IPMCHIFGFGGFAT-GLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVNG 317

Query: 209 LALGPASRPLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYV 268
                +   L  L   V  G  LS+E+  +F +   N    K+   YG TE         
Sbjct: 318 ANEINSKYDLSSLHTVVAGGAPLSREVTEKFVE---NYPKVKILQGYGLTE--STAIAAS 372

Query: 269 LEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRD-SEPGEVWVAGANLAAGYVGGGGA 327
           +   ++   Y    +  P  N    ++D +       ++ GE+W+    +  GY     A
Sbjct: 373 MFNKEETKRYGASGLLAP--NVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEA 430

Query: 328 NRFCDNPHATQPEFQRLYRTGDFGTL-VKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQ 386
                   A+  + +   +TGD   +   G V    R    +K  G++V   E+E  L  
Sbjct: 431 T-------ASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLA 483

Query: 387 VPGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEGKLRSALTHY-MMPQVIEVES 445
            P +    V+     +     + ++  K G+  S   I G +   ++ Y  + +V  + S
Sbjct: 484 HPEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLAS 543

Query: 446 IPLLVNGKVDRQGLLKM 462
           IP   +GK+ R+ L K+
Sbjct: 544 IPKNPSGKILRRELTKL 560


>At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar
           to AMP-binding protein GI:1903034 from [Brassica napus];
           contains Pfam AMP-binding domain PF00501; identical to
           cDNA acyl-activating enzyme 12 (At1g65890) mRNA
           GI:29893228, acyl-activating enzyme 12 [Arabidopsis
           thaliana] GI:29893229
          Length = 578

 Score = 50.0 bits (114), Expect = 6e-06
 Identities = 90/398 (22%), Positives = 156/398 (39%), Gaps = 62/398 (15%)

Query: 112 YTSGSTGIPKGVRLSY-----SAICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVCEIW 166
           YTSG+T  PKGV +S+     S +   + W+  T P      + +W   +   +     W
Sbjct: 193 YTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCP------VYLWTLPMFHCNGWTFTW 246

Query: 167 GPLLHGRALLILSRETTRDPQKLV---NIERLVLVPTLLRSILM--YLALGPASRPLRRL 221
           G    G   + +   T  +  K +   N+  +  VPT+   +L    L L   S P+  L
Sbjct: 247 GTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRSGPVHVL 306

Query: 222 KLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLDLYP-- 279
                 G      L  +  +      G+++ + YG TE  G V +   E  D+ +  P  
Sbjct: 307 ----TGGSPPPAALVKKVQRL-----GFQVMHAYGLTEATGPVLF--CEWQDEWNRLPEN 355

Query: 280 ---TIPIGRPLDNCAIYLLDEELSPTRDSEP------GEVWVAGANLAAGYVGGGGANRF 330
               +   + L    +  +D     T++S P      GE+ + G+++  GY+        
Sbjct: 356 QQMELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYL-------- 407

Query: 331 CDNPHATQPEFQRLY-RTGDFGTL-VKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQVP 388
             NP AT   F+  +  +GD G +   G V    R+   +   G  +  +EVE  + + P
Sbjct: 408 -KNPKATYEAFKHGWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYP 466

Query: 389 GVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEGKL-----------RSALTHYMM 437
            V +  V+             FV  + G  T+ +  E KL           R  L H+M 
Sbjct: 467 KVLETAVVAMPHPTWGETPCAFVVLEKG-ETNNEDREDKLVTKERDLIEYCRENLPHFMC 525

Query: 438 P-QVIEVESIPLLVNGKVDRQGLLKMYENTNNNDDAEV 474
           P +V+ ++ +P   NGK+ +  L  + +     D+  V
Sbjct: 526 PRKVVFLDELPKNGNGKILKPKLRDIAKGLVAEDEVNV 563


>At1g20500.1 68414.m02554 4-coumarate--CoA ligase family /
           4-coumaroyl-CoA synthase family similar to SP|Q42524 and
           SP|Q9S725; contains Pfam AMP-binding enzyme domain
           PF00501
          Length = 412

 Score = 49.2 bits (112), Expect = 1e-05
 Identities = 72/313 (23%), Positives = 117/313 (37%), Gaps = 23/313 (7%)

Query: 24  LATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVIYDDSADPSMFKESGVPFASFEELAQ 83
           L+    GA +         G I+  + D+ P+LV       P +     V     +E+ Q
Sbjct: 107 LSVMSLGAVFTTANTLNTSGEISKQIADSNPTLVFTTRQLAPKLPVAISVVLTD-DEVYQ 165

Query: 84  EATSHSA----------DEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNR 133
           E TS              EP        +  D  A++LY+SG+TG  KGV  S+  +   
Sbjct: 166 ELTSAIRVVGILSEMVKKEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAH 225

Query: 134 LWWQFRTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHGRALLILSRETTRDPQKLVNIE 193
           +          D   IC      T+   +    G +  G  ++IL R    D    V   
Sbjct: 226 VARFISDNLKRDDIFICTVPMFHTY-GLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKH 284

Query: 194 R---LVLVPTLLRSILMYLALGPASRPLRRLKLWVCSGETLSKELASEFFKYFGNEDGYK 250
           R   L L P +L +++    L  A   L  LK   C G  LSKE+   F + +   D   
Sbjct: 285 RATALALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVD--- 341

Query: 251 LANFYGSTEVMGDVTYYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRD-SEPGE 309
           +   Y  TE  G   +     +++   Y T   G    +    ++D         ++ GE
Sbjct: 342 ILQGYALTESNGGGAF--TNSAEESRRYGT--AGTLTSDVEARIVDPNTGRFMGINQTGE 397

Query: 310 VWVAGANLAAGYV 322
           +W+ G +++ G +
Sbjct: 398 LWLKGPSISKGKI 410


>At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative /
           acetate-CoA ligase, putative similar to SP|P27550
           (Escherichia coli) and gi:8439651 (Homo sapiens);
           contains Pfam AMP-binding enzyme domain PF00501
          Length = 693

 Score = 45.6 bits (103), Expect = 1e-04
 Identities = 88/373 (23%), Positives = 148/373 (39%), Gaps = 35/373 (9%)

Query: 110 VLYTSGSTGIPKGVR------LSYSAICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVC 163
           +LYTSGSTG PKGV       + Y+A   +  + +++         C W T  ++V    
Sbjct: 307 LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITGHSYVT--- 363

Query: 164 EIWGPLLHGRALLILSRETTR-DPQKLVNIERLVLV------PTLLRSILMYLALGPASR 216
             +GP+L+G  +++        DP +  +I     V      PTL+RS++          
Sbjct: 364 --YGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFVTRH 421

Query: 217 PLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLD 276
             + L++    GE ++      FF   G +    +++ +  TE  G      L G+    
Sbjct: 422 SRKSLRVLGSVGEPINPSAWRWFFNVVG-DSRCPISDTWWQTET-GGFMITPLPGA---- 475

Query: 277 LYPTIPIGR--PLDNCAIYLLDEELSPTRDSEPGEVWVAGANLAAGYVGGGGANRFCDNP 334
            +P  P     P       ++DE+ +       G + V G+   A     G   R+    
Sbjct: 476 -WPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERY--ET 532

Query: 335 HATQPEFQRLYRTGDFGTLVK-GAVLYAGRTDAQVKIRGHRVDLLEVERALAQVPGVDKC 393
              +P F   Y +GD  +  K G     GR D  + + GHR+   EVE AL   P   + 
Sbjct: 533 TYFKP-FAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQCAEA 591

Query: 394 VVLCYGLDRGNPEILGFVTTKPGARTSAQ---HIEGKLRSALTHYMMPQVIE-VESIPLL 449
            V+    +     I  FVT   G   S +    +   +R+ +  +  P  I     +P  
Sbjct: 592 AVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKT 651

Query: 450 VNGKVDRQGLLKM 462
            +GK+ R+ L K+
Sbjct: 652 RSGKIMRRILRKI 664


>At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 /
           4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
           4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
           (4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
          Length = 561

 Score = 45.2 bits (102), Expect = 2e-04
 Identities = 80/378 (21%), Positives = 149/378 (39%), Gaps = 21/378 (5%)

Query: 91  DEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRLWWQF---RTFPYSDSE 147
           DE    +    +  D  A + ++SG+TG+PKGV L++ ++   +  Q        Y  S 
Sbjct: 191 DETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSN 250

Query: 148 EICVWKTALTFVDSVCEI-WGPLLHGRALLILSRETTRDPQKLVNIERLVLVPTLLRSIL 206
           ++ +    L  + S+  +    L  G  +L++ +        L+   R V +  L+  ++
Sbjct: 251 DVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHR-VTIAALVPPLV 309

Query: 207 MYLALGPA--SRPLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDV 264
           + LA  P   S  L  ++  +     L KEL     +         L   YG TE  G V
Sbjct: 310 IALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQA---ILGQGYGMTEA-GPV 365

Query: 265 TYYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRD-SEPGEVWVAGANLAAGYVG 323
               L  + +     +   G  + N  + ++  E   +   ++PGE+ + G  +   Y+ 
Sbjct: 366 LSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLN 425

Query: 324 GGGANRFCDNPHATQPEFQRLYRTGDFGTLVK-GAVLYAGRTDAQVKIRGHRVDLLEVER 382
              A        AT  E   L+ TGD G + +   +    R    +K +G +V   E+E 
Sbjct: 426 DPEAT------SATIDEEGWLH-TGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELES 478

Query: 383 ALAQVPGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEGKLRSALTHY-MMPQVI 441
            L     +    V+    +      + FV    G   + + ++  +   +  Y  + +V 
Sbjct: 479 LLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVF 538

Query: 442 EVESIPLLVNGKVDRQGL 459
            V SIP   +GK+ R+ L
Sbjct: 539 FVASIPKSPSGKILRKDL 556


>At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase family
           protein similar to SP|Q42524 and SP|Q9S725; contains
           Pfam AMP-binding enzyme domain PF00501
          Length = 447

 Score = 44.4 bits (100), Expect = 3e-04
 Identities = 74/330 (22%), Positives = 121/330 (36%), Gaps = 31/330 (9%)

Query: 12  PHFFYFSTIMALLATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVIYDDSADPSMFKES 71
           P+  Y   +   L+    GA           G I+  +  + P+LV       P +    
Sbjct: 97  PNSIYIPVVC--LSVMSLGAVVTTANTLNTSGEISKQIAQSNPTLVFTTSQLAPKLAAAI 154

Query: 72  GVPFASFE-ELAQEATSHSA-----DEPMDAETLAP-----LTSDSIAIVLYTSGSTGIP 120
            V     E E   E TS         E M  ET        +  D  A++LY+SG+TG  
Sbjct: 155 SVVLTDEEDEKRVELTSGVRVVGILSEMMKKETSGQRVRDRVNQDDTAMMLYSSGTTGTS 214

Query: 121 KGVRLSYSAICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHGRALLILSR 180
           KGV  S+  +   +          D   +C      +F   +    G +  G  ++IL R
Sbjct: 215 KGVISSHRNLTAYVAKYIDDKWKRDEIFVCTVPMFHSF-GLLAFAMGSVASGSTVVILRR 273

Query: 181 ETTRDPQKLVNIER---LVLVPTLLRSILMYLALGPASRPLRRLKLWVCSGETLSKELAS 237
               D  + V   +   L L P +L +++       A   L  L+   C G  LSKE+  
Sbjct: 274 FGLDDMMQAVEKYKATILSLAPPVLVAMINGADQLKAKYDLTSLRKVRCGGAPLSKEVMD 333

Query: 238 EFFKYFGNED---GYKLANFYGSTEVMGDVTYYVLEGSDQLDLYPTIPIGRPLDNCAIYL 294
            F + +   +   GY L   +GS      V   +  G+          +G         +
Sbjct: 334 SFLEKYPTVNIFQGYALTESHGSGASTESVEESLKYGA----------VGLLSSGIEARI 383

Query: 295 LDEELSPTRD-SEPGEVWVAGANLAAGYVG 323
           +D +       ++PGE+W+ G +++ GY G
Sbjct: 384 VDPDTGRVMGVNQPGELWLKGPSISKGYFG 413


>At1g21540.1 68414.m02694 AMP-binding protein, putative strong
           similarity to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501; identical to cDNA adenosine monophosphate
           binding protein 9 AMPBP9 (AMPBP9) GI:20799726
          Length = 550

 Score = 42.3 bits (95), Expect = 0.001
 Identities = 92/438 (21%), Positives = 161/438 (36%), Gaps = 44/438 (10%)

Query: 45  ITHILKDAEPSLVIYDDSADPSMFKESGVPFASFEELAQEATSHSADEPMDAETLAPLTS 104
           ++ + ++ +P LV+ DD  +      S    + F +  Q             + + P T 
Sbjct: 132 VSFMRQNEKPHLVLLDDDQEDGSLSPSAA--SDFLDTYQGVMERGDSR---FKWIRPQTE 186

Query: 105 DSIAIVLYTSGSTGIPKGVRLSYSAI-----CNRLWWQFRTFPYSDSEEICVWKTALTFV 159
               I+ YTSG+T  PKGV LS+ AI      + L W F   P      + +W   +   
Sbjct: 187 WQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRP------VYLWTLPMFHA 240

Query: 160 DSVCEIWGPLLHGRALLILSRETTRDPQKLV---NIERLVLVPTLLRSILMYLALGPASR 216
           +     WG    G   +   R        L+   ++  +   P +L  +  Y    P+ +
Sbjct: 241 NGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNY----PSRK 296

Query: 217 PLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGS-DQL 275
           PL+     + +G      + S          G+ + + YG TE  G V     +   D L
Sbjct: 297 PLKNPVQVMTAGAPPPAAIISR-----AETLGFNVGHGYGLTETGGPVVSCAWKAEWDHL 351

Query: 276 DLYPTIPIGRPLDNCAIYLLDEELSPTRDSEPGEVWVAGANLAAGYV---GGGGANRFCD 332
           D    +   R      +  +       RD   G+  V    ++ G +   GG     +  
Sbjct: 352 D---PLERARLKSRQGVRTIGFAEVDVRDPRTGKS-VEHDGVSVGEIVLKGGSVMLGYYK 407

Query: 333 NPHATQPEFQR--LYRTGDFGTLVK-GAVLYAGRTDAQVKIRGHRVDLLEVERALAQVPG 389
           +P  T    +    + +GD G + + G +    R+   +   G  +   EVE  L   P 
Sbjct: 408 DPEGTAACMREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPV 467

Query: 390 VDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEGKLR----SALTHYMMP-QVIEVE 444
           V +  V+             FV+ K  +  +    E ++R    + L  YM+P +VI  E
Sbjct: 468 VKEAAVVAKPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQE 527

Query: 445 SIPLLVNGKVDRQGLLKM 462
            +P    GK+ +  L +M
Sbjct: 528 ELPKTSTGKIQKFLLRQM 545


>At1g21530.1 68414.m02693 AMP-binding protein, putative strong
           similarity to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501; identical to cDNA adenosine monophosphate
           binding protein 10 AMPBP10 (AMPBP10)  GI:20799728
          Length = 547

 Score = 41.5 bits (93), Expect = 0.002
 Identities = 90/427 (21%), Positives = 157/427 (36%), Gaps = 41/427 (9%)

Query: 53  EPSLVIYDDSADPSMFKESGVPFASFEELAQEATSHSADEPMDAETLAPLTSDSIAIVLY 112
           +P LV+ DD  +      S     ++EE+ +   S         + + P T     ++ Y
Sbjct: 140 KPHLVLLDDDQENDSSSASDF-LDTYEEIMERGNSRF-------KWIRPQTEWQPMVLNY 191

Query: 113 TSGSTGIPKGVRLSYSAICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVCEIWGPLLHG 172
           TSG+T  PKGV LS+ AI          +    +  + +W   +   +     WG    G
Sbjct: 192 TSGTTSSPKGVVLSHRAIFMLTVSSLLDWSV-PNRPVYLWTLPMFHANGWGYTWGTAAVG 250

Query: 173 RALLILSRETTRDPQKLV---NIERLVLVPTLLRSILMYLALGPASRPLRRLKLWVCSGE 229
              +   R        L+   N+  +   P +L  ++ Y    P S PL+   +   SG 
Sbjct: 251 ATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINY----PLSTPLKNPVM--TSGA 304

Query: 230 TLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGS-DQLDLYPTIPIGRPLD 288
                + S          G+ +++ YG TE  G V     +   D LD    +   R   
Sbjct: 305 PPPATIISR-----AESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLD---PLERARLKS 356

Query: 289 NCAIYLLDEELSPTRDSEPGEVWVAGANLAAGYVGGGGAN---RFCDNPHATQPEFQR-- 343
              +  L       RD + G+  V    ++ G +   G++    +  +P  T    +   
Sbjct: 357 RQGVRTLGFTEVDVRDRKTGKS-VKHDGVSVGEIVFRGSSVMLGYYKDPQGTAACMREDG 415

Query: 344 LYRTGDFGTLVK-GAVLYAGRTDAQVKIRGHRVDLLEVERALAQVPGVDKCVVLCYGLDR 402
            + +GD G + K G +    R+   +   G  +   E+E  L   P V +  V+      
Sbjct: 416 WFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKPDKM 475

Query: 403 GNPEILGFVTTK------PGARTSAQHIEGKLRSALTHYMMP-QVIEVESIPLLVNGKVD 455
                  FV+ K           + + I    ++ L  YM+P +VI  E +P    GK+ 
Sbjct: 476 WGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTGKIQ 535

Query: 456 RQGLLKM 462
           +  L +M
Sbjct: 536 KFLLRQM 542


>At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein /
           4-coumaroyl-CoA synthase family protein similar to
           gi:112801 from Petroselinum crispum, GB:AAD40664 from
           [Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559
           (1991)); contains Pfam AMP-binding enzyme domain PF00501
          Length = 542

 Score = 40.3 bits (90), Expect = 0.005
 Identities = 91/431 (21%), Positives = 163/431 (37%), Gaps = 24/431 (5%)

Query: 41  PQGRITHILKDAEPSLV--IYDDSADPSMFKESGVPFASF-EELAQEATS-----HSADE 92
           P   ++ I K  E S    I  D+ +    K  G+P     EE  + A +      + D+
Sbjct: 110 PTALVSEIKKQVEASGARGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDK 169

Query: 93  PMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSY-SAICNRLWWQFRTFPYSDSEEICV 151
             D +    L +D  A+  ++SG+TG+ KGV L++ + I N     F        + + +
Sbjct: 170 CGDTDNEEILQTDLCALP-FSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTL 228

Query: 152 WKTALTFVDSVCEIWGPLLHGRALLI-LSRETTRDPQKLVNIERLVLVPTLLRSILMYLA 210
                  +  +  I    +  +  ++ +SR   R     +    +   P ++  I++ L 
Sbjct: 229 GLIPFFHIYGIVGICCATMKNKGKVVAMSRYDLRIFLNALIAHEVSFAP-IVPPIILNLV 287

Query: 211 LGPASRPLRRLKLWVCSGETLSKELASEFFKYFGNE-DGYKLANFYGSTEVMGDVTYYVL 269
             P        KL + S  T +  LA E    F  +    ++   YG TE    +T    
Sbjct: 288 KNPIVDEFDLSKLKLQSVMTAAAPLAPELLTAFEAKFPNVQVQEAYGLTE-HSCITLTHG 346

Query: 270 EGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRDSEP-GEVWVAGANLAAGYVGGGGAN 328
           +      +     +G  L N  +  +D +   +      GE+ V    +  GY      N
Sbjct: 347 DPEKGQGIAKRNSVGFILPNLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYF----MN 402

Query: 329 RFCDNPHATQPEFQRLYRTGDFGTLVK-GAVLYAGRTDAQVKIRGHRVDLLEVERALAQV 387
           +  +    T  E Q    TGD G +   G +    R    +K +G +V   E+E  L   
Sbjct: 403 K--EETDKTIDE-QGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTH 459

Query: 388 PGVDKCVVLCYGLDRGNPEILGFVTTKPGARTSAQHIEGKLRSALTHYMMPQVIE-VESI 446
           P V+   V+    +         V   P A    + I   + + + HY   + +  V+SI
Sbjct: 460 PSVEDVAVVPLPDEEAGEIPAACVVINPKATEKEEDILNFVAANVAHYKKVRAVHFVDSI 519

Query: 447 PLLVNGKVDRQ 457
           P  ++GK+ R+
Sbjct: 520 PKSLSGKIMRR 530


>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
           protein / long-chain acyl-CoA synthetase family protein
           (LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
           LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
           HMM hit: AMP-binding enzymes PF00501
          Length = 691

 Score = 39.9 bits (89), Expect = 0.006
 Identities = 15/30 (50%), Positives = 26/30 (86%), Gaps = 1/30 (3%)

Query: 101 PLTSDSIAIVLYTSGSTGIPKGVRLSYSAI 130
           PL++D +A+++YTSGSTG+PKGV +++  +
Sbjct: 242 PLSAD-VAVIMYTSGSTGLPKGVMMTHGNV 270



 Score = 30.7 bits (66), Expect = 3.9
 Identities = 31/119 (26%), Positives = 51/119 (42%), Gaps = 9/119 (7%)

Query: 283 IGRPLDNCAIYLLDEE----LSPTRDSEPGEVWVAGANLAAGYVGGGGANRFCDNPHATQ 338
           +G PL    + L+D      L+  +    GE+ + G+N+  GY       +     +   
Sbjct: 469 VGAPLPCSFVKLVDWAEGGYLTSDKPMPRGEIVIGGSNITLGYFKNEEKTK---EVYKVD 525

Query: 339 PEFQRLYRTGDFGTL-VKGAVLYAGRTDAQVKIR-GHRVDLLEVERALAQVPGVDKCVV 395
            +  R + TGD G     G +    R    VK++ G  V L +VE AL+  P V+  +V
Sbjct: 526 EKGMRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALSISPYVENIMV 584


>At5g16340.1 68418.m01910 AMP-binding protein, putative similar to
           AMP-binding protein GI:1903034 from [Brassica napus];
           contains Pfam AMP-binding domain PF00501; identical to
           adenosine monophosphate binding protein 6 AMPBP6
           (AMPBP6) GI:20799720
          Length = 550

 Score = 39.1 bits (87), Expect = 0.011
 Identities = 52/224 (23%), Positives = 95/224 (42%), Gaps = 25/224 (11%)

Query: 248 GYKLANFYGSTEVMGDVT-------YYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEE-- 298
           G+ +++ YG TE  G +        +  L  SD+  L     + R +    I ++D E  
Sbjct: 321 GFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARLKARQGV-RTVGFTEIDVVDPESG 379

Query: 299 LSPTRDSEP-GEVWVAGANLAAGYVGGGGANRFCDNPHATQPEFQR-LYRTGDFGTLVKG 356
           LS  R+ E  GE+ + G+++  GY+          +P  T+   +   + TGD G +   
Sbjct: 380 LSVERNGETVGEIVMRGSSVMLGYL---------KDPVGTEKALKNGWFYTGDVGVIHSD 430

Query: 357 AVLYAGRTDAQVKIRG-HRVDLLEVERALAQVPGVDKCVVLCYGLDRGNPEILGFVTTKP 415
             L        + I G   V  +EVE  L  +P V++  V+    +        FV+ K 
Sbjct: 431 GYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDEFWGETPCAFVSLKN 490

Query: 416 G--ARTSAQHIEGKLRSALTHYMMPQVIE-VESIPLLVNGKVDR 456
           G   + + + +    R  +  YM+P+ +  ++ +P    GKV +
Sbjct: 491 GFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 534


>At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase family
           protein similar to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501; identical to cDNA adenosine monophosphate
           binding protein 7 AMPBP7 (AMPBP7)  GI:20799722
          Length = 569

 Score = 38.7 bits (86), Expect = 0.015
 Identities = 74/362 (20%), Positives = 126/362 (34%), Gaps = 31/362 (8%)

Query: 112 YTSGSTGIPKGVRLSYS-----AICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVCEIW 166
           YTSG+T  PKGV L +      A+ N L W  +         + +W   +   +  C  W
Sbjct: 204 YTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQ------DGAVYLWTLPMFHCNGWCFPW 257

Query: 167 GPLLHGRALLILSRETTRDPQKLV---NIERLVLVPTLLRSILMYLALGPASRPLRRLKL 223
              +     + L + T ++   ++    +      P +L +I+      P    +  L  
Sbjct: 258 SLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVN----APKEDTILPLPH 313

Query: 224 WVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLDLYPTIPI 283
            V      +    S  F    N+ G+++A+ YG +E  G  T  V     + D  P    
Sbjct: 314 TVHVMTAGAAPPPSVLFSM--NQKGFRVAHTYGLSETYGPST--VCAWKPEWDSLPPETQ 369

Query: 284 GRPLDNCAIYLLDEELSPTRDSEPGEVWVAGANLAAGYVGGGG--ANRFCDNPHATQPEF 341
            +      +     E     D++ G+   A    A   V  G      +  NP A +  F
Sbjct: 370 AKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKETF 429

Query: 342 Q-RLYRTGDFGTL-VKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQVPGVDKCVVLCYG 399
               + +GD         +    R+   +   G  +  +EVE  +   P V +  V+   
Sbjct: 430 AGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVARP 489

Query: 400 LDRGNPEILGFVTTKP-----GARTSAQHIEGKLRSALTHYMMPQVIEVESIPLLVNGKV 454
            +R       FVT K           AQ I    R  L  Y +P+ +    +P    GK+
Sbjct: 490 DERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATGKI 549

Query: 455 DR 456
            +
Sbjct: 550 QK 551


>At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase /
           long-chain acyl-CoA synthetase (LACS6) strong similarity
           to AMP-binding protein (MF39P) gi:1617274 from Brassica
           napus, similar to putative long-chain-fatty-acid--CoA
           ligase (brain isozyme) GB:P33124 [Rattus norvegicus];
           contains Pfam AMP-binding enzyme domain PF00501;
           identical to cDNA AtLACS6 for long-chain acyl-CoA
           synthetase GI:22531705
          Length = 701

 Score = 38.7 bits (86), Expect = 0.015
 Identities = 22/64 (34%), Positives = 33/64 (51%), Gaps = 7/64 (10%)

Query: 65  PSMFKESGVPFASFEELAQEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVR 124
           PS+   SGV   S+  L  +  S+        +   P   D +A + YTSG+TG PKGV 
Sbjct: 228 PSLPSSSGVKVVSYSVLLNQGRSNP-------QRFFPPKPDDVATICYTSGTTGTPKGVV 280

Query: 125 LSYS 128
           L+++
Sbjct: 281 LTHA 284


>At5g27600.1 68418.m03305 AMP-binding protein, putative similar to
           AMP-binding protein (MF39P) gi:1617274 from Brassica
           napus, long-chain-fatty-acid--CoA ligase - Brassica
           napus, EMBL:Z72152; contains Pfam AMP-binding enzyme
           domain PF00501
          Length = 700

 Score = 38.3 bits (85), Expect = 0.019
 Identities = 21/63 (33%), Positives = 34/63 (53%), Gaps = 7/63 (11%)

Query: 65  PSMFKESGVPFASFEELAQEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVR 124
           PS+ + +GV   S+++L  +  S            +P   + IA + YTSG+TG PKGV 
Sbjct: 228 PSLPRGTGVTIVSYQKLLSQGRS-------SLHPFSPPKPEDIATICYTSGTTGTPKGVV 280

Query: 125 LSY 127
           L++
Sbjct: 281 LTH 283


>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
           long-chain acyl-CoA synthetase nearly identical to acyl
           CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
          Length = 665

 Score = 37.1 bits (82), Expect = 0.045
 Identities = 35/131 (26%), Positives = 54/131 (41%), Gaps = 20/131 (15%)

Query: 20  IMALLATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVIYDDSADPSMFK-ESGVP---- 74
           I+A+ A    G  YVP+  S     +  I+  AE SLV   +    S+   + G      
Sbjct: 118 IIAMEACMSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLK 177

Query: 75  -FASFEELAQEATSHSADE--------------PMDAETLAPLTSDSIAIVLYTSGSTGI 119
              SF E++      + ++               +D   L       I  ++YTSG+TG 
Sbjct: 178 TIVSFGEVSSTQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGE 237

Query: 120 PKGVRLSYSAI 130
           PKGV L+ +AI
Sbjct: 238 PKGVILNNAAI 248


>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
           similarity to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501
          Length = 545

 Score = 36.7 bits (81), Expect = 0.059
 Identities = 89/430 (20%), Positives = 157/430 (36%), Gaps = 32/430 (7%)

Query: 45  ITHILKDAEPSLVIYDDSADPSMFKESGVPFASFEELAQEATSHSADEPMDAETLAPLTS 104
           ++ + KD  P LVI +D  D      + + F    E   E         +  + + P + 
Sbjct: 131 VSFLPKDERPRLVILNDGNDMPSSSSADMDFLDTYEGFMERGD------LRFKWVRPKSE 184

Query: 105 DSIAIVLYTSGSTGIPKGVRLSY-----SAICNRLWWQFRTFPYSDSEEICVWKTALTFV 159
            +  ++ YTSG+T  PKGV  S+     S I + L W     P      + +W   +   
Sbjct: 185 WTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRP------VYLWTLPMFHA 238

Query: 160 DSVCEIWGPLLHGRALLILSRETTRDPQKLVNIERLVLVPTLLRSILMYLALGPASRPLR 219
           +     W     G   + ++R        L++ +  V        +L  L   PA +PL+
Sbjct: 239 NGWSYTWATAAVGARNICVTRVDVPTIFNLID-KYQVTHMCAAPMVLNMLTNHPAQKPLQ 297

Query: 220 RLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLDLYP 279
                + +G      + S+         G+ +++ YG TE  G V    L+   + D   
Sbjct: 298 SPVKVMTAGAPPPATVISK-----AEALGFDVSHGYGMTETGGLVVSCALK--PEWDRLE 350

Query: 280 TIPIGRPLDNCAIYLLDEELSPTRDSEPGE-VWVAGANLA-AGYVGGGGANRFCDNPHAT 337
                +      I          RD   G+ V   GA +    + GG     +  +P  T
Sbjct: 351 PDERAKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGT 410

Query: 338 QPEFQR--LYRTGDFGTL-VKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQVPGVDKCV 394
               +    + TGD G +   G +    R+   V   G  +   E+E  L   P + +  
Sbjct: 411 AASMREDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAA 470

Query: 395 VLCYGLDRGNPEILGFVTTK-PGARTSAQHIEGKLRSALTHYMMPQ-VIEVESIPLLVNG 452
           V+             FV+ K      + + I    ++ L  YM+P+ V+ +E +P    G
Sbjct: 471 VVAKPDKMWGETPCAFVSLKYHDGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTG 530

Query: 453 KVDRQGLLKM 462
           K+ +  L +M
Sbjct: 531 KIQKFLLRQM 540


>At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase,
           putative / long-chain acyl-CoA synthetase, putative
           similar to acyl-CoA synthetase (MF7P) gi:1617270 from
           Brassica napus
          Length = 666

 Score = 36.3 bits (80), Expect = 0.078
 Identities = 37/131 (28%), Positives = 60/131 (45%), Gaps = 20/131 (15%)

Query: 20  IMALLATWKAGAAYVPMEPSFPQGRITHILKDAEPSLVIYDDSADPSMFK---------E 70
           I+++ A    G   VP+  +   G +  I+  AE S+   ++   P +FK         +
Sbjct: 118 IISMEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMK 177

Query: 71  SGVPFASFE-ELAQEATS-----HSADEPMDA----ETLAPLTSDS-IAIVLYTSGSTGI 119
           + V F   + E  +EA       HS DE +      +   P+   S I  ++YTSG+TG 
Sbjct: 178 TVVSFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGD 237

Query: 120 PKGVRLSYSAI 130
           PKGV +S  +I
Sbjct: 238 PKGVMISNESI 248


>At3g23790.1 68416.m02990 AMP-binding protein, putative similar to
           AMP-binding protein GB:CAA96521 from [Brassica napus]
           (Plant Mol. Biol. (1997) 33 (5), 911-922); contains Pfam
           AMP-binding enzyme domain PF00501; identical to cDNA
           acyl-CoA synthetase-like protein  GI:20799732
          Length = 722

 Score = 36.3 bits (80), Expect = 0.078
 Identities = 14/30 (46%), Positives = 23/30 (76%)

Query: 105 DSIAIVLYTSGSTGIPKGVRLSYSAICNRL 134
           D IA ++YTSG+TG PKGV L++  + +++
Sbjct: 263 DDIATIMYTSGTTGNPKGVMLTHQNLLHQI 292


>At4g14070.1 68417.m02172 AMP-binding protein, putative similar to
           AMP-binding protein [gi:1617272] from Brassica napus;
           contains Pfam AMP-binding enzyme domain PF00501;
           identical to cDNA acyl-CoA synthetase-like protein
           GI:20799730
          Length = 727

 Score = 35.9 bits (79), Expect = 0.10
 Identities = 14/33 (42%), Positives = 24/33 (72%)

Query: 102 LTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRL 134
           + SD  A ++YTSG+TG PKGV L++  + +++
Sbjct: 276 IDSDDTAAIMYTSGTTGNPKGVMLTHRNLLHQI 308


>At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearly
           identical to acyl-activating enzyme 17 [Arabidopsis
           thaliana] GI:29893266; similar to acetyl-CoA synthetase
           [SP|P27095] from Methanothrix soehngenii; contains Pfam
           AMP-binding enzyme domain PF00501; identical to cDNA
           acyl-activating enzyme 17 (At5g23050) GI:29893265
          Length = 721

 Score = 35.5 bits (78), Expect = 0.14
 Identities = 84/386 (21%), Positives = 143/386 (37%), Gaps = 47/386 (12%)

Query: 110 VLYTSGSTGIPKGV---RLSYSAICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVCEIW 166
           +L++SG+TG PK +    +S        W            ++  W T L ++     ++
Sbjct: 354 ILFSSGTTGEPKAIPWTNISPLKSAADAWCHLDV----QRGDVVAWPTNLGWMMGPWLVY 409

Query: 167 GPLLHGRALLILSRETTRDP--QKLV---NIERLVLVPTLLRSILMYLALGPASRPLRRL 221
             L++G A + L   +   P   K V    +  L ++P+++R+     +   +     R+
Sbjct: 410 ASLING-ACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRT--WQNSNSTSGYDWSRI 466

Query: 222 KLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLDLYPTI 281
           +   C G T       E+    G      +  + G TE+ G      L     L  + T 
Sbjct: 467 R---CFGSTGEASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAAFSTA 523

Query: 282 PIGRPLDNCAIYLLDEELSPTRDSEPGEVWVAG-ANLAAGYVGGGGANRFCDNPH----- 335
            +G     C +++LDE      DS P   + AG   LA      G ++   +  H     
Sbjct: 524 AMG-----CKLFILDE------DSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYF 572

Query: 336 ATQPEFQR--LYRTGD-FGTLVKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQV-PGVD 391
              P FQ   L R GD F    KG     GR D  + + G +V  +E+ER    V   V 
Sbjct: 573 QGMPTFQGQILRRHGDLFERTSKGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVL 632

Query: 392 KCVVLCYGLDRGNPEILGFVT-------TKPGARTSAQHIEGKLRSALTH-YMMPQVIEV 443
           +   +      G PE L           + P      +    +++  L   + +  V+ +
Sbjct: 633 ETAAIGVPPPSGGPEQLVIAVVFKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTL 692

Query: 444 ESIPLLVNGKVDRQGLLKMYENTNNN 469
            S+P     KV R+ L +    T  N
Sbjct: 693 PSLPRTATNKVMRRVLRQQLTQTGLN 718


>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
           protein / long-chain acyl-CoA synthetase family protein
           (LACS8) similar to LACS 4 [SP|O35547] from Rattus
           norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
           contains Pfam HMM hit: AMP-binding enzymes PF00501
          Length = 720

 Score = 35.5 bits (78), Expect = 0.14
 Identities = 11/25 (44%), Positives = 22/25 (88%)

Query: 103 TSDSIAIVLYTSGSTGIPKGVRLSY 127
           + + +A++++TSGSTG+PKGV +++
Sbjct: 272 SKNGVAVIMFTSGSTGLPKGVMITH 296


>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
           protein / long-chain acyl-CoA synthetase family protein
           (LACS8) similar to LACS 4 [SP|O35547] from Rattus
           norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
           contains Pfam HMM hit: AMP-binding enzymes PF00501
          Length = 720

 Score = 35.5 bits (78), Expect = 0.14
 Identities = 11/25 (44%), Positives = 22/25 (88%)

Query: 103 TSDSIAIVLYTSGSTGIPKGVRLSY 127
           + + +A++++TSGSTG+PKGV +++
Sbjct: 272 SKNGVAVIMFTSGSTGLPKGVMITH 296


>At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase family
           protein / long-chain acyl-CoA synthetase family protein
           similar to GI:1617270 (MF7P) and gi:1617628 (MF45P) from
           [Brassica napus] ; contains Pfam AMP-binding enzyme
           domain PF00501
          Length = 660

 Score = 32.7 bits (71), Expect = 0.96
 Identities = 14/34 (41%), Positives = 23/34 (67%)

Query: 97  ETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAI 130
           E   P  + +I  ++YTSG++G PKGV L++ A+
Sbjct: 212 EDTNPPKAFNICTIMYTSGTSGDPKGVVLTHQAV 245


>At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 /
           4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
           4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
           (4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
          Length = 495

 Score = 32.3 bits (70), Expect = 1.3
 Identities = 50/239 (20%), Positives = 96/239 (40%), Gaps = 12/239 (5%)

Query: 91  DEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLSYSAICNRLWWQF---RTFPYSDSE 147
           DE    +    +  D  A + ++SG+TG+PKGV L++ ++   +  Q        Y  S 
Sbjct: 191 DETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSN 250

Query: 148 EICVWKTALTFVDSVCEI-WGPLLHGRALLILSRETTRDPQKLVNIERLVLVPTLLRSIL 206
           ++ +    L  + S+  +    L  G  +L++ +        L+   R V +  L+  ++
Sbjct: 251 DVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHR-VTIAALVPPLV 309

Query: 207 MYLALGPA--SRPLRRLKLWVCSGETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDV 264
           + LA  P   S  L  ++  +     L KEL     +         L   YG TE  G V
Sbjct: 310 IALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQA---ILGQGYGMTEA-GPV 365

Query: 265 TYYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRD-SEPGEVWVAGANLAAGYV 322
               L  + +     +   G  + N  + ++  E   +   ++PGE+ + G  +   Y+
Sbjct: 366 LSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYL 424


>At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase /
           long-chain acyl-CoA synthetase nearly identical to
           acyl-CoA synthetase (MF7P) from Brassica napus
           [gi:1617270]
          Length = 666

 Score = 31.9 bits (69), Expect = 1.7
 Identities = 13/24 (54%), Positives = 18/24 (75%)

Query: 107 IAIVLYTSGSTGIPKGVRLSYSAI 130
           I  ++YTSG+TG PKGV +S  +I
Sbjct: 225 ICTIMYTSGTTGDPKGVMISNESI 248


>At5g46840.1 68418.m05771 RNA recognition motif (RRM)-containing
           protein contains InterPro entry IPR000504: RNA-binding
           region RNP-1 (RNA recognition motif) (RRM)
          Length = 501

 Score = 31.5 bits (68), Expect = 2.2
 Identities = 23/74 (31%), Positives = 32/74 (43%), Gaps = 1/74 (1%)

Query: 645 LNFDVRDEPEIELSGGLAKIMGFLEYVEGSVRDTMLPHGKGAVLHSFMMATSERLSARDN 704
           L FDV+DE   +L  G + +   +E V   +RD  L  GKG     F    +  L  +  
Sbjct: 290 LPFDVKDEEVYQLFTGKSNLENSIEAVR-VIRDPHLNIGKGIAYVLFKTREAANLVLKKG 348

Query: 705 VSAIRALELATDRV 718
              +R  EL   RV
Sbjct: 349 YLKLRERELRISRV 362


>At5g35430.1 68418.m04213 expressed protein
          Length = 786

 Score = 31.5 bits (68), Expect = 2.2
 Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 1/51 (1%)

Query: 266 YYVLEGSDQLDLYPTIPIGRPLDNCAIYLLDEELSPTRDSEPGEVWVAGAN 316
           Y  L GS+QL    ++P+ R   +  IYLL+E LS    S+ G +   G N
Sbjct: 496 YVELAGSNQLSKL-SLPLARVCLSNGIYLLNESLSNDSKSDLGSILSVGMN 545


>At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase,
           putative / long-chain acyl-CoA synthetase, putative
           similar to GI:1617270 (MF7P) from [Brassica napus]
          Length = 665

 Score = 31.5 bits (68), Expect = 2.2
 Identities = 13/28 (46%), Positives = 20/28 (71%)

Query: 107 IAIVLYTSGSTGIPKGVRLSYSAICNRL 134
           +  ++YTSG+TG PKGV L+  +I + L
Sbjct: 225 VCTIMYTSGTTGDPKGVLLTNESIIHLL 252


>At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative
           strong similarity to hydroxymethylpyrimidine
           kinase/thiamin-phosphate pyrophosphorylase (BTH1)
           GI:7488455 from [Brassica napus]
          Length = 522

 Score = 31.5 bits (68), Expect = 2.2
 Identities = 14/58 (24%), Positives = 31/58 (53%)

Query: 578 MRDEHKKEVIDMVESSFYEKAELEQFLKHEIETRDYADCIDACWPALLQAGLSVVLND 635
           M  +  + ++D ++++    A + Q  + E ETR++ +   AC       G+S+++ND
Sbjct: 321 MNKKWNRSIVDALKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLIND 378


>At5g39870.1 68418.m04836 hypothetical protein
          Length = 290

 Score = 31.1 bits (67), Expect = 2.9
 Identities = 13/41 (31%), Positives = 24/41 (58%)

Query: 13 HFFYFSTIMALLATWKAGAAYVPMEPSFPQGRITHILKDAE 53
          HF  F+ ++A +  +K  +A+V ++P+ PQ      +KD E
Sbjct: 6  HFLCFTILVATITFFKVASAHVKIKPALPQIEDPLTVKDVE 46


>At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearly
           identical to acyl-activating enzyme 18 [Arabidopsis
           thaliana] GI:29893268; similar to acetyl-CoA synthetase
           [SP|P27095] from Methanothrix soehngenii; contains Pfam
           AMP-binding enzyme domain PF00501l; identical to cDNA
           acyl-activating enzyme 18 (At1g55320) GI: 29893267
          Length = 725

 Score = 30.7 bits (66), Expect = 3.9
 Identities = 83/403 (20%), Positives = 148/403 (36%), Gaps = 35/403 (8%)

Query: 51  DAEPSLVIYDDSADPSMFKESGVPFASFEELAQEATSHSADEPMDAETLAPLTSDSIAIV 110
           +A PS VI   ++   +  +      S+ +    A  HS+ E        P+  +S+  +
Sbjct: 302 EAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLPV--ESVINI 359

Query: 111 LYTSGSTGIPKGVRLSYSAICNRLWWQFRTFPYSDSEEICVWKTALTFVDSVCEIWGPLL 170
           L++SG+TG PK +  +  +        +           C W T L +V     ++   L
Sbjct: 360 LFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYC-WPTNLGWVMGPTLMFSCFL 418

Query: 171 HGRALLILSRETT-RDPQKL-VNIERLVLVPTLLRSILMYLALGPASRPLRRLKLWVCSG 228
            G  L + S     R   K    +  L  VP+L+++      +   +    ++K +  +G
Sbjct: 419 TGATLALYSGSPLGRGFGKFDAGVTVLGTVPSLVKTWKRTNCMEGLN--WTKIKFFATTG 476

Query: 229 ETLSKELASEFFKYFGNEDGYKLANFYGSTEVMGDVTYYVLEGSDQLDLYPTIPIGRPLD 288
           E  +     +        D   +    G TE+    + Y++    Q   +       P  
Sbjct: 477 EASN---VDDVLWLSSKADYKPVIECCGGTEL---ASSYIIGSPLQPQAFGA--FSTPSM 528

Query: 289 NCAIYLLDEELSPTRDSEP--GEVWVAGANLAAGYVGGGGANRFCDNPH-----ATQPEF 341
              I + DE   P  D +P  GEV +   +L       G  +R  +  H        P +
Sbjct: 529 TTRIIIFDENGVPYPDDQPCTGEVGLFPQHL-------GATDRLLNANHDEVYFKGMPMY 581

Query: 342 Q--RLYRTGDF-GTLVKGAVLYAGRTDAQVKIRGHRVDLLEVERALAQVPG-VDKCVVLC 397
           +  RL R GD     V G     GR D  + + G +   +E+ER   Q    + +   + 
Sbjct: 582 KETRLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVT 641

Query: 398 YGLDRGNPEILG-FVTTKPG-ARTSAQHIEGKLRSALTHYMMP 438
                G PE+L  F   K G  + S + ++ K    +   + P
Sbjct: 642 LTPPNGGPELLVIFAVLKEGFKQQSGEELKMKFSRTIQKDLNP 684


>At4g17960.1 68417.m02674 expressed protein
          Length = 154

 Score = 30.3 bits (65), Expect = 5.1
 Identities = 14/47 (29%), Positives = 23/47 (48%)

Query: 48 ILKDAEPSLVIYDDSADPSMFKESGVPFASFEELAQEATSHSADEPM 94
          +LK+ E  + +Y D  +   F+E+G+P   F    Q A   S   P+
Sbjct: 17 VLKNEEDDIDVYGDGGNQQRFRETGLPRKGFGVPVQVAVERSQLGPL 63


>At5g58870.1 68418.m07376 FtsH protease, putative contains
           similarity to cell division protein FtsH homolog 3
           SP:P73437 (EC 3.4.24.-) [strain PCC6803] {Synechocystis
           sp.}
          Length = 806

 Score = 29.5 bits (63), Expect = 9.0
 Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 3/46 (6%)

Query: 549 STLGEVLSKMSTDPNGG---ADSKEATFTAVPMRDEHKKEVIDMVE 591
           ST G++ ++ S  P GG    D +  TF  V   DE K+E+ ++VE
Sbjct: 301 STTGQLRTRKSGGPGGGKVSGDGETITFADVAGVDEAKEELEEIVE 346


>At5g36860.1 68418.m04416 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain
          Length = 1204

 Score = 29.5 bits (63), Expect = 9.0
 Identities = 16/44 (36%), Positives = 23/44 (52%)

Query: 83  QEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLS 126
           Q   SH + EP  +   AP+T+   +  + T GSTG  K  RL+
Sbjct: 505 QTIPSHDSREPPTSMDKAPVTAKKPSRRMSTKGSTGTRKSSRLT 548


>At4g08880.1 68417.m01464 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain; similar to At3g24380, At5g36840,
           At5g35010, At3g42740, At4g05290, At2g14770, At3g43390,
           At2g05560, At1g34730, At1g27790, At1g34740, At1g27780,
           At5g36850, At3g42730, At1g52020, At3g24390, At4g05280,
           At1g25886, At4g03300
          Length = 1463

 Score = 29.5 bits (63), Expect = 9.0
 Identities = 19/69 (27%), Positives = 30/69 (43%)

Query: 58  IYDDSADPSMFKESGVPFASFEELAQEATSHSADEPMDAETLAPLTSDSIAIVLYTSGST 117
           +++D  D    +    P    +   Q   SH + EP  +   AP+T    +  + T GST
Sbjct: 344 MHEDMKDFVRDQIRAAPDPKGKRTEQTIPSHDSREPPTSMDKAPVTGKKPSRRMSTKGST 403

Query: 118 GIPKGVRLS 126
           G  K  RL+
Sbjct: 404 GTRKSSRLT 412


>At3g42730.1 68416.m04462 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain; similar to At3g24380, At5g36840,
           At5g35010, At3g42740, At4g05290, At2g14770, At3g43390,
           At2g05560, At4g08880, At1g34730, At1g27790, At1g34740,
           At1g27780, At5g36850, At1g52020, At3g24390, At4g05280,
           At1g25886, At4g03300
          Length = 1314

 Score = 29.5 bits (63), Expect = 9.0
 Identities = 16/44 (36%), Positives = 23/44 (52%)

Query: 83  QEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLS 126
           Q   SH + EP  +   AP+T+   +  + T GSTG  K  RL+
Sbjct: 503 QTIPSHDSREPPTSMDKAPVTAKKPSRRMSTKGSTGTRKSSRLT 546


>At2g14770.2 68415.m01669 Ulp1 protease family protein similar to
           At3g24380, At5g36840, At5g35010, At3g42740, At4g05290,
           At2g14770, At3g43390, At2g05560, At4g08880, At1g34730,
           At1g27790, At1g34740, At1g27780, At5g36850, At3g42730,
           At1g52020, At3g24390,  At4g05280, At1g25886, At4g03300;
           contains Pfam profile PF02902: Ulp1 protease family,
           C-terminal catalytic domain
          Length = 1158

 Score = 29.5 bits (63), Expect = 9.0
 Identities = 16/44 (36%), Positives = 23/44 (52%)

Query: 83  QEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLS 126
           Q   SH + EP  +   AP+T+   +  + T GSTG  K  RL+
Sbjct: 505 QTIPSHDSREPPTSMDKAPVTAKKPSRRMSTKGSTGTRKSSRLT 548


>At2g14770.1 68415.m01668 Ulp1 protease family protein similar to
           At3g24380, At5g36840, At5g35010, At3g42740, At4g05290,
           At2g14770, At3g43390, At2g05560, At4g08880, At1g34730,
           At1g27790, At1g34740, At1g27780, At5g36850, At3g42730,
           At1g52020, At3g24390,  At4g05280, At1g25886, At4g03300;
           contains Pfam profile PF02902: Ulp1 protease family,
           C-terminal catalytic domain
          Length = 1139

 Score = 29.5 bits (63), Expect = 9.0
 Identities = 16/44 (36%), Positives = 23/44 (52%)

Query: 83  QEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLS 126
           Q   SH + EP  +   AP+T+   +  + T GSTG  K  RL+
Sbjct: 505 QTIPSHDSREPPTSMDKAPVTAKKPSRRMSTKGSTGTRKSSRLT 548


>At1g34740.1 68414.m04319 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain; similar to At3g24380, At5g36840,
           At5g35010, At3g42740, At4g05290, At2g14770, At3g43390,
           At2g05560, At4g08880, At1g34730, At1g27790, At1g27780,
           At5g36850, At3g42730, At1g52020, At3g24390, At4g05280,
           At1g25886, At4g03300
          Length = 1383

 Score = 29.5 bits (63), Expect = 9.0
 Identities = 16/44 (36%), Positives = 23/44 (52%)

Query: 83  QEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLS 126
           Q   SH + EP  +   AP+T+   +  + T GSTG  K  RL+
Sbjct: 485 QTIPSHDSREPPTSMDKAPVTAKKPSRRMSTKGSTGTRKSSRLT 528


>At1g27780.1 68414.m03399 Ulp1 protease family protein similar to
           At3g24380, At5g36840, At5g35010, At3g42740, At4g05290,
           At2g14770, At3g43390, At2g05560, At4g08880, At1g34730,
           At1g27790, At1g34740, At5g36850, At3g42730, At1g52020,
           At3g24390, At4g05280, At1g25886, At4g03300; contains
           Pfam profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain
          Length = 1468

 Score = 29.5 bits (63), Expect = 9.0
 Identities = 16/44 (36%), Positives = 23/44 (52%)

Query: 83  QEATSHSADEPMDAETLAPLTSDSIAIVLYTSGSTGIPKGVRLS 126
           Q   SH + EP  +   AP+T+   +  + T GSTG  K  RL+
Sbjct: 503 QTIPSHDSREPPTSMDKAPVTAKKPSRRMSTKGSTGTRKSSRLT 546


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.319    0.136    0.407 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,254,180
Number of Sequences: 28952
Number of extensions: 783609
Number of successful extensions: 1838
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 39
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 1753
Number of HSP's gapped (non-prelim): 81
length of query: 783
length of database: 12,070,560
effective HSP length: 87
effective length of query: 696
effective length of database: 9,551,736
effective search space: 6648008256
effective search space used: 6648008256
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 63 (29.5 bits)

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