BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000025-TA|BGIBMGA000025-PA|undefined
(479 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g45190.1 68416.m04877 SIT4 phosphatase-associated family prot... 41 0.002
At4g29210.1 68417.m04179 gamma-glutamyltranspeptidase family pro... 35 0.10
At1g07990.1 68414.m00871 SIT4 phosphatase-associated family prot... 35 0.14
At5g65470.1 68418.m08233 expressed protein contains Pfam PF03138... 33 0.32
At2g33850.1 68415.m04155 expressed protein contains 1 transmembr... 33 0.32
At2g03220.1 68415.m00275 galactoside 2-alpha-L-fucosyltransferas... 31 1.3
At1g09400.1 68414.m01051 12-oxophytodienoate reductase, putative... 31 1.3
At3g57250.1 68416.m06373 emsy N terminus domain-containing prote... 31 2.2
At2g28360.1 68415.m03447 SIT4 phosphatase-associated family prot... 31 2.2
At5g27530.1 68418.m03295 glycoside hydrolase family 28 protein /... 30 2.9
At3g03970.3 68416.m00418 expressed protein 30 3.9
At3g03970.2 68416.m00417 expressed protein 30 3.9
At3g03970.1 68416.m00416 expressed protein 30 3.9
At5g58440.1 68418.m07319 phox (PX) domain-containing protein sim... 29 5.1
At1g48650.1 68414.m05445 helicase domain-containing protein cont... 29 5.1
At4g15120.1 68417.m02323 VQ motif-containing protein contains PF... 29 6.8
At3g16370.1 68416.m02071 GDSL-motif lipase/hydrolase family prot... 29 6.8
At2g47500.1 68415.m05929 kinesin motor protein-related 29 6.8
At1g52570.1 68414.m05935 phospholipase D alpha 2 / PLD alpha 2 (... 29 6.8
At1g30470.1 68414.m03724 SIT4 phosphatase-associated family prot... 29 6.8
>At3g45190.1 68416.m04877 SIT4 phosphatase-associated family protein
contains Pfam profile: PF04499 SIT4
phosphatase-associated protein
Length = 830
Score = 41.1 bits (92), Expect = 0.002
Identities = 26/89 (29%), Positives = 44/89 (49%), Gaps = 5/89 (5%)
Query: 80 LQDFIRSLENVTEKEALLNKTMGYVENLIDNVLNYERIKIIDGVEIKIKNDTKVVDKERK 139
L DF+ +L NVT E +L T G + L R+KI++ + + +K ++ KE
Sbjct: 305 LGDFV-ALLNVTSDEKILPTTYGQLRP----PLGSHRLKIVEFIAVLLKTRSEATGKELA 359
Query: 140 ENNDIEEARGLFSKYTYEYRLYQKIKSFI 168
+ I LF +Y Y L+ +++S I
Sbjct: 360 SSGAIRRVLDLFFEYPYNNALHHQVESII 388
>At4g29210.1 68417.m04179 gamma-glutamyltranspeptidase family
protein similar to SP|P07314
Gamma-glutamyltranspeptidase precursor (EC 2.3.2.2)
(Gamma- glutamyltransferase) (GGT) {Rattus norvegicus};
contains Pfam profilePF01019:
Gamma-glutamyltranspeptidase
Length = 637
Score = 35.1 bits (77), Expect = 0.10
Identities = 24/74 (32%), Positives = 39/74 (52%), Gaps = 5/74 (6%)
Query: 77 PSTLQDFIRSLE-NVTEKEALLNKTMGYVENLIDNVLNYERIKIIDGVEIKIKNDTKVVD 135
P+ LQ F+ N+ KEA+ ++ LI NV++YE I+G I + DTK+
Sbjct: 515 PAVLQVFLNCFVLNMKPKEAV--ESARIYHRLIPNVVSYENFTTINGDHIGVSEDTKMFL 572
Query: 136 KERKENNDIEEARG 149
ER ++++E G
Sbjct: 573 AER--GHELKELSG 584
>At1g07990.1 68414.m00871 SIT4 phosphatase-associated family protein
contains Pfam profile: PF04499 SIT4
phosphatase-associated protein
Length = 802
Score = 34.7 bits (76), Expect = 0.14
Identities = 15/57 (26%), Positives = 30/57 (52%)
Query: 112 LNYERIKIIDGVEIKIKNDTKVVDKERKENNDIEEARGLFSKYTYEYRLYQKIKSFI 168
L R+KI++ + + +K ++ KE + I+ LF +Y Y L+ +++S I
Sbjct: 332 LGKHRLKIVEFIAVLLKTRSEAAQKELVSSGTIKRTLDLFFEYPYNNALHHQVESII 388
>At5g65470.1 68418.m08233 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown;
Length = 504
Score = 33.5 bits (73), Expect = 0.32
Identities = 18/41 (43%), Positives = 26/41 (63%), Gaps = 1/41 (2%)
Query: 95 ALLNKTMGYVENLIDNVLNYERIKIIDGVEI-KIKNDTKVV 134
+L +KT GYV+ +D LN +R+ I D V + KI N T V+
Sbjct: 80 SLPDKTAGYVQVFLDGGLNQQRMGICDAVAVAKILNATLVI 120
>At2g33850.1 68415.m04155 expressed protein contains 1 transmembrane
domain; similar to Protein E6 (Swiss-Prot:Q01197)
[Gossypium hirsutum]
Length = 267
Score = 33.5 bits (73), Expect = 0.32
Identities = 22/75 (29%), Positives = 32/75 (42%), Gaps = 7/75 (9%)
Query: 242 YPPANMDDTNAYNND-NKDFMGYETNPTGTYAYPQDYATEGNDMEPG----NIDMSRYGA 296
Y +N T+ +ND N + GY T Y P Y TE + EP N ++ R G
Sbjct: 141 YENSNAYGTDKRDNDINDPYKGYSNKDTSYYENPNTYGTEKREKEPAYRGYNNNVERQGM 200
Query: 297 GGPKVTYLPSKNSYY 311
+ Y+ + YY
Sbjct: 201 SDTR--YMANGKYYY 213
>At2g03220.1 68415.m00275 galactoside 2-alpha-L-fucosyltransferase /
xyloglucan alpha-(1,2)-fucosyltransferase (FUT1) (FT1)
identical to SP|Q9SWH5 Galactoside
2-alpha-L-fucosyltransferase (EC 2.4.1.69) (Xyloglucan
alpha-(1,2)-fucosyltransferase) (AtFUT1) {Arabidopsis
thaliana}
Length = 558
Score = 31.5 bits (68), Expect = 1.3
Identities = 18/84 (21%), Positives = 35/84 (41%)
Query: 323 IFQKIPASSMILSNYDPFYSPLLSRLDGIFARLGLAPSENAVTDSPQMGGQSLSDMKLEA 382
+F + A SMI + + ++ + G+ P+ + +GG S ++
Sbjct: 51 VFSVLVAFSMIFHQHPSDSNRIMGFAEARVLDAGVFPNVTNINSDKLLGGLLASGFDEDS 110
Query: 383 CREQLICLMYANPAKYAPYSNLVS 406
C + + Y P+ Y P S L+S
Sbjct: 111 CLSRYQSVHYRKPSPYKPSSYLIS 134
>At1g09400.1 68414.m01051 12-oxophytodienoate reductase, putative
similar to OPR1 [GI:3882355] and OPR2 [GI:3882356]
Length = 324
Score = 31.5 bits (68), Expect = 1.3
Identities = 25/89 (28%), Positives = 39/89 (43%), Gaps = 3/89 (3%)
Query: 4 KKQTSRWTGYRKAQWPIENHTLILMYILLFAARFNCQDLSINEETKNNTVDINKSDCHST 63
K+Q W A + +H I L A R + QD N E+ ++ D +D S
Sbjct: 66 KEQIEAWKPIVDA---VHSHGGIFFCQLWHAGRVSHQDCQPNGESPVSSTDKPFADDPSN 122
Query: 64 ESSNINVLVSDKAPSTLQDFIRSLENVTE 92
E + L +D+ P+ + DF + N TE
Sbjct: 123 EFTPPRRLRTDEIPTIINDFRLAARNATE 151
>At3g57250.1 68416.m06373 emsy N terminus domain-containing protein
/ ENT domain-containing protein contains Pfam profile
PF03735: ENT domain
Length = 142
Score = 30.7 bits (66), Expect = 2.2
Identities = 15/61 (24%), Positives = 29/61 (47%)
Query: 80 LQDFIRSLENVTEKEALLNKTMGYVENLIDNVLNYERIKIIDGVEIKIKNDTKVVDKERK 139
+ D +++L N+ ++ A + M Y L+ ++ K DGV +KN + D +
Sbjct: 40 ISDDVKNLLNIEQESAEEKRKMAYEATLLAATFSHSLAKDRDGVLATLKNQLHITDDFHR 99
Query: 140 E 140
E
Sbjct: 100 E 100
>At2g28360.1 68415.m03447 SIT4 phosphatase-associated family protein
contains Pfam profile: PF04499 SIT4
phosphatase-associated protein
Length = 826
Score = 30.7 bits (66), Expect = 2.2
Identities = 14/57 (24%), Positives = 29/57 (50%)
Query: 112 LNYERIKIIDGVEIKIKNDTKVVDKERKENNDIEEARGLFSKYTYEYRLYQKIKSFI 168
L R+KI++ + + +K+ + E + I+ LF +Y Y L+ +++S I
Sbjct: 383 LGKHRLKIVEFIAVLLKSGNEAAGTELAISGTIKRILELFFEYPYNNALHHQVESII 439
>At5g27530.1 68418.m03295 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Lycopersicon esculentum] GI:4325090;
contains PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 458
Score = 30.3 bits (65), Expect = 2.9
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 2/44 (4%)
Query: 38 NCQDLSINEETKNNTVD--INKSDCHSTESSNINVLVSDKAPST 79
NC +L+IN T ++ I+ +CH S IN+L + +P+T
Sbjct: 170 NCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNT 213
>At3g03970.3 68416.m00418 expressed protein
Length = 554
Score = 29.9 bits (64), Expect = 3.9
Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Query: 59 DCHSTESSNINVLVSDKAPSTLQDFIRSLENVTEKEALLNKTMGYVENLIDNVLNYERIK 118
D H T SN+ +V D + F+ L +V KE L ++ GY +L +++ +K
Sbjct: 21 DSHKTAMSNLRSIVKDLDAKVVHVFVAQLSDV--KEIGL-ESGGYTVSLFEDLARAHGVK 77
Query: 119 IIDGVEI 125
I ++I
Sbjct: 78 IAPHIDI 84
>At3g03970.2 68416.m00417 expressed protein
Length = 554
Score = 29.9 bits (64), Expect = 3.9
Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Query: 59 DCHSTESSNINVLVSDKAPSTLQDFIRSLENVTEKEALLNKTMGYVENLIDNVLNYERIK 118
D H T SN+ +V D + F+ L +V KE L ++ GY +L +++ +K
Sbjct: 21 DSHKTAMSNLRSIVKDLDAKVVHVFVAQLSDV--KEIGL-ESGGYTVSLFEDLARAHGVK 77
Query: 119 IIDGVEI 125
I ++I
Sbjct: 78 IAPHIDI 84
>At3g03970.1 68416.m00416 expressed protein
Length = 554
Score = 29.9 bits (64), Expect = 3.9
Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Query: 59 DCHSTESSNINVLVSDKAPSTLQDFIRSLENVTEKEALLNKTMGYVENLIDNVLNYERIK 118
D H T SN+ +V D + F+ L +V KE L ++ GY +L +++ +K
Sbjct: 21 DSHKTAMSNLRSIVKDLDAKVVHVFVAQLSDV--KEIGL-ESGGYTVSLFEDLARAHGVK 77
Query: 119 IIDGVEI 125
I ++I
Sbjct: 78 IAPHIDI 84
>At5g58440.1 68418.m07319 phox (PX) domain-containing protein
similar to SP|O60749 Sorting nexin 2 {Homo sapiens};
contains Pfam profile PF00787: PX domain
Length = 587
Score = 29.5 bits (63), Expect = 5.1
Identities = 21/65 (32%), Positives = 36/65 (55%), Gaps = 5/65 (7%)
Query: 282 NDMEPGNIDMSRYGAGGP-KVTYL--PSKNSYYKNQMSTANNYKIFQKIPASSMILSNYD 338
+DME N+D+ G P K++ + + NS Y++ MST +N + P + MI ++ D
Sbjct: 20 DDME--NLDLGVDGGDHPLKISDVNGDTSNSGYRSAMSTLSNVRDPLSPPPTVMIPADSD 77
Query: 339 PFYSP 343
P +P
Sbjct: 78 PLLAP 82
>At1g48650.1 68414.m05445 helicase domain-containing protein
contains similarity to DEIH-box RNA/DNA helicase
[Arabidopsis thaliana] GI:5881579; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain, PF00035:
Double-stranded RNA binding motif
Length = 1197
Score = 29.5 bits (63), Expect = 5.1
Identities = 13/40 (32%), Positives = 21/40 (52%)
Query: 125 IKIKNDTKVVDKERKENNDIEEARGLFSKYTYEYRLYQKI 164
++ K D +VV +ERK+ D + L ++ R Y KI
Sbjct: 145 LRNKEDQEVVSRERKDRRDFDHISALATRMGLHSRQYSKI 184
>At4g15120.1 68417.m02323 VQ motif-containing protein contains
PF05678: VQ motif
Length = 193
Score = 29.1 bits (62), Expect = 6.8
Identities = 28/93 (30%), Positives = 41/93 (44%), Gaps = 11/93 (11%)
Query: 221 SFGKILGKGISHLSA--TSSQASYP--PANMDDTNAYNN---DNKDFMGYETNPTGTYAY 273
+FG G S S+ T+ +S P AN+ + A+N + +M +N T +Y
Sbjct: 87 AFGSSPSSGFSLTSSDPTAGVSSSPWQYANLQNQMAHNELMQQQRPYMFSSSNNVSTLSY 146
Query: 274 PQDYATEGNDMEPGNIDMSRYGAGGPKVTYLPS 306
P AT+G + SR G GG Y PS
Sbjct: 147 PNAVATDGF----VGAEESREGGGGGGGGYAPS 175
>At3g16370.1 68416.m02071 GDSL-motif lipase/hydrolase family protein
similar to family II lipases EXL3 GI:15054386, EXL1
GI:15054382, EXL2 GI:15054384 from [Arabidopsis
thaliana]; contains Pfam profile: PF00657 Lipase
Acylhydrolase with GDSL-like motif
Length = 353
Score = 29.1 bits (62), Expect = 6.8
Identities = 17/57 (29%), Positives = 27/57 (47%)
Query: 195 FLPLLIGGQVLIKTILFAMFLPSILGSFGKILGKGISHLSATSSQASYPPANMDDTN 251
FL L + + I I FA +P+I+ ++ G ++ T +A YPP D N
Sbjct: 8 FLLLTLVSTLSILQISFAQLVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGRDFAN 64
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 29.1 bits (62), Expect = 6.8
Identities = 16/45 (35%), Positives = 23/45 (51%)
Query: 43 SINEETKNNTVDINKSDCHSTESSNINVLVSDKAPSTLQDFIRSL 87
SIN E + D NK S+ S+ + ++SDK P + I SL
Sbjct: 209 SINNEKAPSENDSNKLSSPSSLSTLVRAVLSDKKPEDVPKLIESL 253
>At1g52570.1 68414.m05935 phospholipase D alpha 2 / PLD alpha 2
(PLDALPHA2) (PLD2) / choline phosphatase 2 identical to
phospholipase D alpha 2 ( PLD alpha 2) SP:Q9SSQ9 from
[Arabidopsis thaliana]
Length = 810
Score = 29.1 bits (62), Expect = 6.8
Identities = 21/77 (27%), Positives = 38/77 (49%), Gaps = 2/77 (2%)
Query: 104 VENLIDNVLNYERIKIIDGVEIKIKNDTKVVDKERKENNDIEEARGLFSKYT-YEYRLYQ 162
V+ +D +L+ E+ I G +I +K V+K++ N I+ A+ YT + R
Sbjct: 122 VDRWVD-ILDNEKNPIAGGSKIHVKLQYFGVEKDKNWNRGIKSAKFPGVPYTFFSQRRGC 180
Query: 163 KIKSFIDTHVLSISLPK 179
K+ + D H+ +PK
Sbjct: 181 KVSLYQDAHIPGNFVPK 197
>At1g30470.1 68414.m03724 SIT4 phosphatase-associated family protein
contains similarity to copper chaperone homolog CCH
GB:AAF15286 GI:6525011 from [Glycine max]; contains Pfam
profile PF04499: SIT4 phosphatase-associated protein
Length = 811
Score = 29.1 bits (62), Expect = 6.8
Identities = 19/82 (23%), Positives = 39/82 (47%), Gaps = 4/82 (4%)
Query: 87 LENVTEKEALLNKTMGYVENLIDNVLNYERIKIIDGVEIKIKNDTKVVDKERKENNDIEE 146
L NV+ E +L T G ++ L R+KI++ + + + ++ +KE ++
Sbjct: 308 LLNVSSAEGVLLTTYGKLQP----PLGKHRLKIVEFISVLLTVGSEAAEKEVIRLGAVKR 363
Query: 147 ARGLFSKYTYEYRLYQKIKSFI 168
LF +Y Y L+ +++ I
Sbjct: 364 VLDLFFEYPYNNFLHHHVENVI 385
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.133 0.385
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,415,006
Number of Sequences: 28952
Number of extensions: 498640
Number of successful extensions: 1476
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1462
Number of HSP's gapped (non-prelim): 22
length of query: 479
length of database: 12,070,560
effective HSP length: 84
effective length of query: 395
effective length of database: 9,638,592
effective search space: 3807243840
effective search space used: 3807243840
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 61 (28.7 bits)
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