GeneModel BLAST Result against uniref50

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000023-TA|BGIBMGA000023-PA|IPR003254|Insect immunity
protein and cecropin, IPR000875|Cecropin
         (63 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P04142 Cluster: Cecropin-B precursor; n=16; Obtectomera...   128   2e-29
UniRef50_P01507 Cluster: Cecropin-A precursor; n=17; Ditrysia|Re...    95   3e-19
UniRef50_A6BMG0 Cluster: Cecropin A; n=1; Plutella xylostella|Re...    64   5e-10
UniRef50_Q2WGL2 Cluster: Antibacterial peptide; n=4; Obtectomera...    49   2e-05
UniRef50_P48821 Cluster: Antibacterial peptide enbocin precursor...    49   2e-05
UniRef50_Q5W8G6 Cluster: Cecropin; n=1; Acalolepta luxuriosa|Rep...    42   0.002
UniRef50_P01511 Cluster: Cecropin-D; n=6; Obtectomera|Rep: Cecro...    42   0.002
UniRef50_Q8MUF4 Cluster: Cecropin-B precursor; n=18; Culicidae|R...    42   0.003
UniRef50_P14954 Cluster: Cecropin-A1/A2 precursor; n=44; Schizop...    34   0.63 
UniRef50_Q4STF9 Cluster: Chromosome undetermined SCAF14235, whol...    32   2.6  
UniRef50_Q2HWM6 Cluster: Myosin heavy chain c064N04-L1-2; n=5; T...    32   2.6  
UniRef50_Q0Q027 Cluster: Putative defense protein; n=1; Antherae...    31   4.5  

>UniRef50_P04142 Cluster: Cecropin-B precursor; n=16;
          Obtectomera|Rep: Cecropin-B precursor - Bombyx mori
          (Silk moth)
          Length = 63

 Score =  128 bits (310), Expect = 2e-29
 Identities = 63/63 (100%), Positives = 63/63 (100%)

Query: 1  MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAKA 60
          MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAKA
Sbjct: 1  MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAKA 60

Query: 61 IGK 63
          IGK
Sbjct: 61 IGK 63


>UniRef50_P01507 Cluster: Cecropin-A precursor; n=17;
          Ditrysia|Rep: Cecropin-A precursor - Hyalophora
          cecropia (Cecropia moth)
          Length = 64

 Score = 94.7 bits (225), Expect = 3e-19
 Identities = 41/63 (65%), Positives = 53/63 (84%)

Query: 1  MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAKA 60
          MNF++I  FVFA + AL+M +AAPEP+WK+FKKIEK+G+NIRDGI+KAGPA+ V+G A  
Sbjct: 1  MNFSRIFFFVFACLTALAMVNAAPEPKWKLFKKIEKVGQNIRDGIIKAGPAVAVVGQATQ 60

Query: 61 IGK 63
          I K
Sbjct: 61 IAK 63


>UniRef50_A6BMG0 Cluster: Cecropin A; n=1; Plutella
          xylostella|Rep: Cecropin A - Plutella xylostella
          (Diamondback moth)
          Length = 66

 Score = 64.1 bits (149), Expect = 5e-10
 Identities = 32/64 (50%), Positives = 44/64 (68%), Gaps = 3/64 (4%)

Query: 1  MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVK-AGPAIEVLGSAK 59
          M  + I  FVF    A++  SAAP  RWK FKK+EK+GRNIR+GI++  GPA+ V+G A 
Sbjct: 1  MKLSNIFFFVFMAFFAVASVSAAP--RWKPFKKLEKVGRNIRNGIIRYNGPAVAVIGQAT 58

Query: 60 AIGK 63
          +I +
Sbjct: 59 SIAR 62


>UniRef50_Q2WGL2 Cluster: Antibacterial peptide; n=4;
          Obtectomera|Rep: Antibacterial peptide - Bombyx mori
          (Silk moth)
          Length = 66

 Score = 49.2 bits (112), Expect = 2e-05
 Identities = 25/61 (40%), Positives = 37/61 (60%), Gaps = 5/61 (8%)

Query: 1  MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAKA 60
          M F KI   VF  ++ + + S A    W  FK++E +G+ +RD I+ AGPAI+VL  AK 
Sbjct: 1  MYFTKI---VFVAIICIMIVSCASA--WDFFKELEGVGQRVRDSIISAGPAIDVLQKAKG 55

Query: 61 I 61
          +
Sbjct: 56 L 56


>UniRef50_P48821 Cluster: Antibacterial peptide enbocin precursor;
          n=5; Ditrysia|Rep: Antibacterial peptide enbocin
          precursor - Bombyx mori (Silk moth)
          Length = 59

 Score = 48.8 bits (111), Expect = 2e-05
 Identities = 23/61 (37%), Positives = 39/61 (63%), Gaps = 5/61 (8%)

Query: 1  MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAKA 60
          MNF +I+ F+F +V A    +A+ +P W IFK+IE+     RD ++ AGPA+  + +A +
Sbjct: 1  MNFTRIIFFLFVVVFA----TASGKP-WNIFKEIERAVARTRDAVISAGPAVRTVAAATS 55

Query: 61 I 61
          +
Sbjct: 56 V 56


>UniRef50_Q5W8G6 Cluster: Cecropin; n=1; Acalolepta luxuriosa|Rep:
          Cecropin - Acalolepta luxuriosa (Udo longicorn beetle)
          Length = 60

 Score = 42.3 bits (95), Expect = 0.002
 Identities = 24/56 (42%), Positives = 35/56 (62%), Gaps = 3/56 (5%)

Query: 9  FVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGP-AIEVLGSAKAIGK 63
          FVFAL + L++T  A    +  FK+IEK+G+NIR+   ++ P  +   G AK IGK
Sbjct: 7  FVFALAVLLALTGQAESKNF--FKRIEKVGKNIRNAAERSLPTVVGYAGVAKQIGK 60


>UniRef50_P01511 Cluster: Cecropin-D; n=6; Obtectomera|Rep:
          Cecropin-D - Antheraea pernyi (Chinese oak silk moth)
          Length = 36

 Score = 41.9 bits (94), Expect = 0.002
 Identities = 15/36 (41%), Positives = 24/36 (66%)

Query: 28 WKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAKAIGK 63
          W  FK++E+ G+ +RD I+ AGPA+  +  A A+ K
Sbjct: 1  WNPFKELERAGQRVRDAIISAGPAVATVAQATALAK 36


>UniRef50_Q8MUF4 Cluster: Cecropin-B precursor; n=18;
          Culicidae|Rep: Cecropin-B precursor - Anopheles gambiae
          (African malaria mosquito)
          Length = 60

 Score = 41.5 bits (93), Expect = 0.003
 Identities = 23/63 (36%), Positives = 35/63 (55%), Gaps = 4/63 (6%)

Query: 1  MNFAKILSFV-FALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAK 59
          MNF K+   V  A+++ + +      PRWK  K++EK+GRN+     KA P   V+   K
Sbjct: 1  MNFTKLFILVAIAVLVVVGVQPVDGAPRWKFGKRLEKLGRNVFRAAKKALP---VIAGYK 57

Query: 60 AIG 62
          A+G
Sbjct: 58 ALG 60


>UniRef50_P14954 Cluster: Cecropin-A1/A2 precursor; n=44;
          Schizophora|Rep: Cecropin-A1/A2 precursor - Drosophila
          melanogaster (Fruit fly)
          Length = 63

 Score = 33.9 bits (74), Expect = 0.63
 Identities = 19/47 (40%), Positives = 30/47 (63%), Gaps = 1/47 (2%)

Query: 1  MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVK 47
          MNF  I  FV AL+LA+++  +      KI KKIE++G++ RD  ++
Sbjct: 1  MNFYNIFVFV-ALILAITIGQSEAGWLKKIGKKIERVGQHTRDATIQ 46


>UniRef50_Q4STF9 Cluster: Chromosome undetermined SCAF14235, whole
           genome shotgun sequence; n=14; Eukaryota|Rep: Chromosome
           undetermined SCAF14235, whole genome shotgun sequence -
           Tetraodon nigroviridis (Green puffer)
          Length = 2604

 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/30 (43%), Positives = 19/30 (63%)

Query: 32  KKIEKMGRNIRDGIVKAGPAIEVLGSAKAI 61
           KK  KMG ++ D I+ A P +E  G+AK +
Sbjct: 260 KKESKMGGSLEDQIIAANPLLEAYGNAKTV 289


>UniRef50_Q2HWM6 Cluster: Myosin heavy chain c064N04-L1-2; n=5;
          Takifugu rubripes|Rep: Myosin heavy chain c064N04-L1-2
          - Fugu rubripes (Japanese pufferfish) (Takifugu
          rubripes)
          Length = 86

 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/30 (43%), Positives = 19/30 (63%)

Query: 32 KKIEKMGRNIRDGIVKAGPAIEVLGSAKAI 61
          KK  KMG ++ D I+ A P +E  G+AK +
Sbjct: 11 KKESKMGGSLEDQIIAANPLLEAYGNAKTV 40


>UniRef50_Q0Q027 Cluster: Putative defense protein; n=1; Antheraea
          mylitta|Rep: Putative defense protein - Antheraea
          mylitta (Tasar silkworm)
          Length = 144

 Score = 31.1 bits (67), Expect = 4.5
 Identities = 12/23 (52%), Positives = 19/23 (82%)

Query: 33 KIEKMGRNIRDGIVKAGPAIEVL 55
          ++E +G+ +RD I+ AGPAI+VL
Sbjct: 55 ELEGIGQRVRDSIIIAGPAIDVL 77


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.323    0.137    0.389 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 59,224,267
Number of Sequences: 1657284
Number of extensions: 1590069
Number of successful extensions: 4424
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4410
Number of HSP's gapped (non-prelim): 12
length of query: 63
length of database: 575,637,011
effective HSP length: 43
effective length of query: 20
effective length of database: 504,373,799
effective search space: 10087475980
effective search space used: 10087475980
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 65 (30.3 bits)