SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20184
         (765 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q9VNE2 Cluster: Protein extra bases; n=13; Neoptera|Rep...   153   6e-36
UniRef50_Q7L1Q6 Cluster: Basic leucine zipper and W2 domain-cont...    91   2e-17
UniRef50_UPI000155D2EC Cluster: PREDICTED: similar to MSTP017; n...    88   3e-16
UniRef50_UPI0000504803 Cluster: similar to basic leucine zipper ...    79   2e-13
UniRef50_A2YZC2 Cluster: Putative uncharacterized protein; n=1; ...    49   1e-04
UniRef50_A7PV62 Cluster: Chromosome chr4 scaffold_32, whole geno...    43   0.010
UniRef50_Q9FG63 Cluster: Gb|AAD26879.1; n=10; Magnoliophyta|Rep:...    42   0.013
UniRef50_A1CW26 Cluster: Putative uncharacterized protein; n=3; ...    42   0.022
UniRef50_Q5KI79 Cluster: Putative uncharacterized protein; n=1; ...    41   0.039
UniRef50_UPI000155D313 Cluster: PREDICTED: similar to integrin a...    35   2.5  
UniRef50_Q88A68 Cluster: Putative uncharacterized protein; n=2; ...    35   2.5  
UniRef50_Q6MBP2 Cluster: Putative uncharacterized protein; n=1; ...    34   4.4  
UniRef50_A1FXQ0 Cluster: Beta-lactamase-like; n=11; Gammaproteob...    34   4.4  
UniRef50_P20930 Cluster: Filaggrin; n=18; Catarrhini|Rep: Filagg...    34   4.4  
UniRef50_Q6CFR4 Cluster: Yarrowia lipolytica chromosome B of str...    33   5.9  
UniRef50_Q4PRZ9 Cluster: Dinitrogenase reductase; n=1; unculture...    33   7.8  

>UniRef50_Q9VNE2 Cluster: Protein extra bases; n=13; Neoptera|Rep:
           Protein extra bases - Drosophila melanogaster (Fruit
           fly)
          Length = 422

 Score =  153 bits (370), Expect = 6e-36
 Identities = 91/205 (44%), Positives = 122/205 (59%), Gaps = 6/205 (2%)
 Frame = +1

Query: 100 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKY*TRPDQNS 279
           MSQK E+PVLSGQRIKTRKRDE+EKYDP GFRDA++ GLE+  GDL+   KY      +S
Sbjct: 1   MSQKTERPVLSGQRIKTRKRDEREKYDPTGFRDAVIAGLEKTEGDLEQISKY-----LDS 55

Query: 280 TTDAMAKSYRCTHCWRPAAAGRFGVDGRRIAQDQHLHLLRQ*GYGHHAKFRTGIRETDAS 459
             + +         +    AG   V G  I+QD          +       +        
Sbjct: 56  AGNKLDYRRYGEVLFDILIAGGLLVPGGSISQDGEKPRTSYCIFDAPESMESMRNHEQVF 115

Query: 460 VQVLG--KNVRR--GNEKGSV--YLKGFDPEQRIKLARMTALWIGNGCVPPSVLLVLVNE 621
           V+++   K + +    E G V  ++KGF P +RIKLARMTALW+ NG VPP+VLLVL NE
Sbjct: 116 VKLIRRYKYLEKMFEEEMGKVLLFVKGFTPSERIKLARMTALWLVNGSVPPNVLLVLNNE 175

Query: 622 HLLKDNLAMDFVLEVFAIIKQERGV 696
           HL+KD +A++F+LE+F   KQE+G+
Sbjct: 176 HLIKDGIALEFLLELFQTFKQEKGI 200



 Score =  113 bits (272), Expect = 5e-24
 Identities = 53/76 (69%), Positives = 62/76 (81%)
 Frame = +2

Query: 308 DVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMFE 487
           D+LIAGGLL+PGGS+S DGE P+T+ CIF A E M++MRN EQVFVKL+RRYKYLEKMFE
Sbjct: 71  DILIAGGLLVPGGSISQDGEKPRTSYCIFDAPESMESMRNHEQVFVKLIRRYKYLEKMFE 130

Query: 488 EEMKKVLST*KASIPN 535
           EEM KVL   K   P+
Sbjct: 131 EEMGKVLLFVKGFTPS 146



 Score = 36.7 bits (81), Expect = 0.63
 Identities = 15/17 (88%), Positives = 17/17 (100%)
 Frame = +3

Query: 255 LDSAGSKLDYRRYGEVI 305
           LDSAG+KLDYRRYGEV+
Sbjct: 53  LDSAGNKLDYRRYGEVL 69


>UniRef50_Q7L1Q6 Cluster: Basic leucine zipper and W2
           domain-containing protein 1; n=78; Eumetazoa|Rep: Basic
           leucine zipper and W2 domain-containing protein 1 - Homo
           sapiens (Human)
          Length = 419

 Score = 91.5 bits (217), Expect = 2e-17
 Identities = 67/199 (33%), Positives = 101/199 (50%), Gaps = 10/199 (5%)
 Frame = +1

Query: 103 SQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKY*TRPDQNST 282
           +QK +KP LSGQR KTRKRDEKE++DP  F+D ++QGL   G DL+A  K+         
Sbjct: 3   NQKQQKPTLSGQRFKTRKRDEKERFDPTQFQDCIIQGLTETGTDLEAVAKFLDASGAKLD 62

Query: 283 TDAMAKSYRCTHCWRPAAAGRFGVDGRRIAQDQHLHLLRQ*GYGHHAKFRTGIRETDASV 462
               A++      +    AG     G  +A D    ++R       A  +  +    A  
Sbjct: 63  YRRYAET-----LFDILVAGGMLAPGGTLADD----MMRTDVCVFAA--QEDLETMQAFA 111

Query: 463 QVLGKNVRRGN--EKG--------SVYLKGFDPEQRIKLARMTALWIGNGCVPPSVLLVL 612
           QV  K +RR    EKG         ++LKGF   +R KLA +T + + NG +  S+L  L
Sbjct: 112 QVFNKLIRRYKYLEKGFEDEVKKLLLFLKGFSESERNKLAMLTGVLLANGTLNASILNSL 171

Query: 613 VNEHLLKDNLAMDFVLEVF 669
            NE+L+K+ ++  F +++F
Sbjct: 172 YNENLVKEGVSAAFAVKLF 190



 Score = 85.4 bits (202), Expect = 1e-15
 Identities = 38/67 (56%), Positives = 55/67 (82%)
 Frame = +2

Query: 308 DVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMFE 487
           D+L+AGG+L PGG+++ D    +T+ C+F+A ED++TM+ F QVF KL+RRYKYLEK FE
Sbjct: 72  DILVAGGMLAPGGTLADD--MMRTDVCVFAAQEDLETMQAFAQVFNKLIRRYKYLEKGFE 129

Query: 488 EEMKKVL 508
           +E+KK+L
Sbjct: 130 DEVKKLL 136


>UniRef50_UPI000155D2EC Cluster: PREDICTED: similar to MSTP017; n=1;
           Ornithorhynchus anatinus|Rep: PREDICTED: similar to
           MSTP017 - Ornithorhynchus anatinus
          Length = 349

 Score = 87.8 bits (208), Expect = 3e-16
 Identities = 41/68 (60%), Positives = 53/68 (77%), Gaps = 1/68 (1%)
 Frame = +2

Query: 308 DVLIAGGLLLPGGSVSMDGESPK-TNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMF 484
           DVL+AG +L PGG+   DG+  K T  C+FSA+ED D +RN+ QVF KL+RRYKYLEK F
Sbjct: 70  DVLVAGSMLAPGGTRIDDGDKTKMTKHCVFSADEDHDAIRNYAQVFNKLIRRYKYLEKAF 129

Query: 485 EEEMKKVL 508
           E+E+KK+L
Sbjct: 130 EDEIKKLL 137



 Score = 75.8 bits (178), Expect = 1e-12
 Identities = 34/49 (69%), Positives = 41/49 (83%)
 Frame = +1

Query: 109 KVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 255
           K +KPVL+GQR KTRKRDEKEK++P  FRD+LVQGL  AGGDL+A  K+
Sbjct: 3   KHQKPVLTGQRFKTRKRDEKEKFEPTVFRDSLVQGLNDAGGDLEAVAKF 51


>UniRef50_UPI0000504803 Cluster: similar to basic leucine zipper and
           W2 domains 1 (LOC501543), mRNA; n=1; Rattus
           norvegicus|Rep: similar to basic leucine zipper and W2
           domains 1 (LOC501543), mRNA - Rattus norvegicus
          Length = 346

 Score = 78.6 bits (185), Expect = 2e-13
 Identities = 36/67 (53%), Positives = 53/67 (79%)
 Frame = +2

Query: 308 DVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMFE 487
           D+L+AGG++ PGG+++ D   P T+ C+F+A ED++TM+ F QVF KL R YKYLEK F+
Sbjct: 72  DILVAGGMVAPGGTLA-DDMMP-TDVCVFAAQEDLETMQAFAQVFNKLFRCYKYLEKGFD 129

Query: 488 EEMKKVL 508
           +E+KK+L
Sbjct: 130 DEVKKLL 136



 Score = 63.7 bits (148), Expect = 5e-09
 Identities = 26/50 (52%), Positives = 36/50 (72%)
 Frame = +1

Query: 106 QKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 255
           QK +KP+L+GQR K RKRDEKE +DP  F+D +++GL   G D +A  K+
Sbjct: 4   QKQQKPMLAGQRFKIRKRDEKETFDPTHFQDCIIEGLAETGTDFEAVAKF 53



 Score = 42.3 bits (95), Expect = 0.013
 Identities = 19/54 (35%), Positives = 34/54 (62%)
 Frame = +1

Query: 508 VYLKGFDPEQRIKLARMTALWIGNGCVPPSVLLVLVNEHLLKDNLAMDFVLEVF 669
           V+LKGF   +R K A +T + + NG +   +L  L NE+L+K+ ++  F +++F
Sbjct: 138 VFLKGFSVSERNKFAMLTGVLLANGTLNAFILNSLYNENLVKEVVSAAFAVKIF 191


>UniRef50_A2YZC2 Cluster: Putative uncharacterized protein; n=1;
           Oryza sativa (indica cultivar-group)|Rep: Putative
           uncharacterized protein - Oryza sativa subsp. indica
           (Rice)
          Length = 403

 Score = 48.8 bits (111), Expect = 1e-04
 Identities = 29/67 (43%), Positives = 35/67 (52%)
 Frame = +1

Query: 76  NLLISIYCMSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 255
           +L +S+ C     EKP L GQRIKTRKR+     DP  F DA+VQ      GDL+   K 
Sbjct: 59  DLFVSLKCSK---EKPTLGGQRIKTRKRNIAAPLDPASFSDAIVQIYLDNAGDLELVAKS 115

Query: 256 *TRPDQN 276
               D N
Sbjct: 116 IESSDLN 122


>UniRef50_A7PV62 Cluster: Chromosome chr4 scaffold_32, whole genome
           shotgun sequence; n=1; Vitis vinifera|Rep: Chromosome
           chr4 scaffold_32, whole genome shotgun sequence - Vitis
           vinifera (Grape)
          Length = 237

 Score = 42.7 bits (96), Expect = 0.010
 Identities = 27/59 (45%), Positives = 31/59 (52%)
 Frame = +1

Query: 100 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKY*TRPDQN 276
           MS K E+P L G RIKTRKR+     DP  F DA+VQ      GDL+   K     D N
Sbjct: 164 MSSK-ERPTLGGTRIKTRKRNIAAPLDPATFADAVVQIYLDNAGDLELIAKSIESSDLN 221


>UniRef50_Q9FG63 Cluster: Gb|AAD26879.1; n=10; Magnoliophyta|Rep:
           Gb|AAD26879.1 - Arabidopsis thaliana (Mouse-ear cress)
          Length = 429

 Score = 42.3 bits (95), Expect = 0.013
 Identities = 26/62 (41%), Positives = 31/62 (50%)
 Frame = +1

Query: 97  CMSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKY*TRPDQN 276
           C + +   P LSG RIKTRKR+     DP  F DA+VQ      GDL+   K     D N
Sbjct: 18  CSAARRRNP-LSGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIESSDLN 76

Query: 277 ST 282
            T
Sbjct: 77  FT 78


>UniRef50_A1CW26 Cluster: Putative uncharacterized protein; n=3;
            Trichocomaceae|Rep: Putative uncharacterized protein -
            Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL
            181)(Aspergillus fischerianus (strain ATCC 1020 / DSM
            3700 / NRRL 181))
          Length = 1210

 Score = 41.5 bits (93), Expect = 0.022
 Identities = 26/80 (32%), Positives = 38/80 (47%)
 Frame = +1

Query: 127  LSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKY*TRPDQNSTTDAMAKSY 306
            LSG+  K+  R ++E Y        +  G+ER GG + A Y   T P++ + T A+A   
Sbjct: 1112 LSGKSSKSSLRQQQEGYLTGQSNYTVKVGMERNGGAIHAGYGLSTVPEKQTETSALASFL 1171

Query: 307  RCTHCWRPAAAGRFGVDGRR 366
            R T    P A  R  + G R
Sbjct: 1172 RSTGPPEPPAGRRPSLSGSR 1191


>UniRef50_Q5KI79 Cluster: Putative uncharacterized protein; n=1;
           Filobasidiella neoformans|Rep: Putative uncharacterized
           protein - Cryptococcus neoformans (Filobasidiella
           neoformans)
          Length = 432

 Score = 40.7 bits (91), Expect = 0.039
 Identities = 24/62 (38%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
 Frame = +2

Query: 308 DVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDM-DTMRNFEQVFVKLMRRYKYLEKMF 484
           ++L  GGLL PGGS   D  SP        A +   D ++   +V  ++M+RYKYL+K  
Sbjct: 87  ELLFVGGLLQPGGSYLDDKRSPVYILQPDDAPDAFKDGVKGMIEVLKRVMQRYKYLQKPL 146

Query: 485 EE 490
           EE
Sbjct: 147 EE 148



 Score = 35.1 bits (77), Expect = 1.9
 Identities = 19/49 (38%), Positives = 28/49 (57%)
 Frame = +1

Query: 511 YLKGFDPEQRIKLARMTALWIGNGCVPPSVLLVLVNEHLLKDNLAMDFV 657
           YL  +D + R KLA  TAL      +    L  L  EH++KDN+A++F+
Sbjct: 157 YLPKWDVKSREKLAEATALLTIELQISSRCLQSLAKEHVVKDNVALNFL 205



 Score = 33.9 bits (74), Expect = 4.4
 Identities = 17/33 (51%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
 Frame = +1

Query: 115 EKPVLSGQRIKTRKRDEK--EKYDPNGFRDALV 207
           +KP L+G RIK RK   K   K++P  FRDAL+
Sbjct: 18  KKPSLTGVRIKQRKGQAKATAKFEPEAFRDALL 50


>UniRef50_UPI000155D313 Cluster: PREDICTED: similar to integrin alpha
            E2; n=1; Ornithorhynchus anatinus|Rep: PREDICTED: similar
            to integrin alpha E2 - Ornithorhynchus anatinus
          Length = 1679

 Score = 34.7 bits (76), Expect = 2.5
 Identities = 19/52 (36%), Positives = 27/52 (51%)
 Frame = +3

Query: 585  RASIRAAGPGERTSAEGQPGDGLRAGSVRDHQAGARRHSLVTALKKGQLEGR 740
            R ++R   P    S+EG PG G +  S R    G+RR +LV +  + Q  GR
Sbjct: 1278 RVALRRRPPLAGASSEGPPGWGAQGESSRGSHRGSRREALVPSASRWQPLGR 1329


>UniRef50_Q88A68 Cluster: Putative uncharacterized protein; n=2;
           Gammaproteobacteria|Rep: Putative uncharacterized
           protein - Pseudomonas syringae pv. tomato
          Length = 650

 Score = 34.7 bits (76), Expect = 2.5
 Identities = 32/99 (32%), Positives = 44/99 (44%), Gaps = 8/99 (8%)
 Frame = -1

Query: 594 WRHASVTDPQC--SHARQLD-ALF-GIEAFQVDRTFFISSSNIFSKYLYRRISFTNTCSK 427
           W    ++ P C  +HA  L+ ALF  IE   V + F I    +  K  YR    T+  S+
Sbjct: 66  WPAPEMSRPGCVWTHALLLEPALFESIEDLSVLQAFAIRPKGLVDKERYRE-PLTHDVSQ 124

Query: 426 F----RMVSISSLAEKMQVLVLGDSPSIDTEPPGSSRPP 322
                + V I+ L   +  L  G SPSI+ E PG    P
Sbjct: 125 LVQSPKSVDIAILKRLLLSLYTGGSPSIEVESPGQLDAP 163


>UniRef50_Q6MBP2 Cluster: Putative uncharacterized protein; n=1;
           Candidatus Protochlamydia amoebophila UWE25|Rep:
           Putative uncharacterized protein - Protochlamydia
           amoebophila (strain UWE25)
          Length = 93

 Score = 33.9 bits (74), Expect = 4.4
 Identities = 15/31 (48%), Positives = 19/31 (61%)
 Frame = -3

Query: 523 SLSSRQNLFHFLFEHFFQVLVPTHQFHEYLF 431
           SL +   L HFLF HF Q  V TH FH +++
Sbjct: 60  SLFNSFTLKHFLFSHFIQHKVFTHNFHLFIY 90


>UniRef50_A1FXQ0 Cluster: Beta-lactamase-like; n=11;
           Gammaproteobacteria|Rep: Beta-lactamase-like -
           Stenotrophomonas maltophilia R551-3
          Length = 493

 Score = 33.9 bits (74), Expect = 4.4
 Identities = 18/55 (32%), Positives = 28/55 (50%)
 Frame = -2

Query: 494 FPLRTFFPSTCTDASVSRIPVRNFAWCPYPHWRRRCRCWSWAIRRPSTPNRPAAA 330
           +P+ T      T A+ +R P    +W   PH R  CR WSW++   +  +RPA +
Sbjct: 16  WPVPTARARAMTSAASARSPR---SWL-LPHARPACRAWSWSVAPAAWKSRPACS 66


>UniRef50_P20930 Cluster: Filaggrin; n=18; Catarrhini|Rep: Filaggrin -
            Homo sapiens (Human)
          Length = 4061

 Score = 33.9 bits (74), Expect = 4.4
 Identities = 25/78 (32%), Positives = 36/78 (46%), Gaps = 4/78 (5%)
 Frame = +3

Query: 516  ERLRSRTAHQAGAHDCTVDR*RMRASIRAAGP---GERTSAEGQPGDGLRAGSVRDHQAG 686
            E+L  R+ H    H  T  + R  AS   +G      +T  + Q GDG R    R H+A 
Sbjct: 2479 EQLVDRSGHSGSHHSHTTSQGRSDASHGHSGSRSASRQTRNDEQSGDGSRHSGSRHHEAS 2538

Query: 687  ARRHSL-VTALKKGQLEG 737
            +R  S   + + +GQ EG
Sbjct: 2539 SRADSSGHSQVGQGQSEG 2556



 Score = 33.1 bits (72), Expect = 7.8
 Identities = 25/71 (35%), Positives = 32/71 (45%), Gaps = 7/71 (9%)
 Frame = +3

Query: 516  ERLRSRTAHQAGAHDCTVDR*RMRASIRAAGP---GERTSAEGQPGDGLRAGSVRDHQ-- 680
            E+   R+ H    H  T  + R  AS   +GP     +T  E Q GDG R    R H+  
Sbjct: 1506 EQSVDRSGHSGYHHSHTTPQGRSDASHGQSGPRSASRQTRNEEQSGDGSRHSGSRHHEPS 1565

Query: 681  --AGARRHSLV 707
              AG+ RHS V
Sbjct: 1566 TRAGSSRHSQV 1576


>UniRef50_Q6CFR4 Cluster: Yarrowia lipolytica chromosome B of strain
           CLIB122 of Yarrowia lipolytica; n=1; Yarrowia
           lipolytica|Rep: Yarrowia lipolytica chromosome B of
           strain CLIB122 of Yarrowia lipolytica - Yarrowia
           lipolytica (Candida lipolytica)
          Length = 329

 Score = 33.5 bits (73), Expect = 5.9
 Identities = 18/40 (45%), Positives = 23/40 (57%), Gaps = 6/40 (15%)
 Frame = -1

Query: 657 HEVHRQVVLQQMFVHQDQQ------HGWRHASVTDPQCSH 556
           H+ H Q  LQQ  +HQ QQ      HG+R AS++ PQ  H
Sbjct: 222 HQQHIQQQLQQQQMHQQQQQQQYYPHGYRQASLSPPQHYH 261


>UniRef50_Q4PRZ9 Cluster: Dinitrogenase reductase; n=1; uncultured
           bacterium|Rep: Dinitrogenase reductase - uncultured
           bacterium
          Length = 129

 Score = 33.1 bits (72), Expect = 7.8
 Identities = 16/40 (40%), Positives = 22/40 (55%)
 Frame = +3

Query: 585 RASIRAAGPGERTSAEGQPGDGLRAGSVRDHQAGARRHSL 704
           R  + A GPG  + A  + G G R+G+ R H+ G  RH L
Sbjct: 41  RLILHATGPGHHSVAGRRSGLGGRSGTGRCHEGGISRHPL 80


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 775,309,784
Number of Sequences: 1657284
Number of extensions: 17287199
Number of successful extensions: 71534
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 65445
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 71421
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 63792713725
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -