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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20091
         (706 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P49448 Cluster: Glutamate dehydrogenase 2, mitochondria...    71   2e-11
UniRef50_Q4T019 Cluster: Chromosome undetermined SCAF11390, whol...    71   4e-11
UniRef50_Q9VCN3 Cluster: CG4434-PA; n=3; Sophophora|Rep: CG4434-...    49   1e-04
UniRef50_Q2S0C1 Cluster: Glutamate dehydrogenase, short peptide;...    43   0.008
UniRef50_Q54KB7 Cluster: Glutamate dehydrogenase, NAD(P)+; n=1; ...    39   0.14 
UniRef50_Q24BW7 Cluster: Glutamate/Leucine/Phenylalanine/Valine ...    38   0.32 
UniRef50_Q23ZD8 Cluster: Glutamate/Leucine/Phenylalanine/Valine ...    38   0.32 
UniRef50_Q94IH8 Cluster: Glutamate dhydrogenase; n=5; Viridiplan...    37   0.55 
UniRef50_O96248 Cluster: Putative uncharacterized protein PFB077...    35   1.7  
UniRef50_Q0W8B3 Cluster: Glutamate dehydrogenase; n=2; unculture...    35   2.2  
UniRef50_Q73P03 Cluster: Glutamate dehydrogenase; n=3; Bacteria|...    34   3.9  
UniRef50_A6DTG1 Cluster: Glutamate dehydrogenase/leucine dehydro...    34   3.9  
UniRef50_Q9Y4B6 Cluster: Protein VPRBP; n=26; Fungi/Metazoa grou...    34   3.9  
UniRef50_A0BLL2 Cluster: Chromosome undetermined scaffold_114, w...    33   6.8  

>UniRef50_P49448 Cluster: Glutamate dehydrogenase 2, mitochondrial
           precursor; n=91; Eumetazoa|Rep: Glutamate dehydrogenase
           2, mitochondrial precursor - Homo sapiens (Human)
          Length = 558

 Score = 71.3 bits (167), Expect = 2e-11
 Identities = 40/120 (33%), Positives = 67/120 (55%), Gaps = 2/120 (1%)
 Frame = +3

Query: 330 FFPPSLSSCRRKLVEDLKSRTPIEEKKKKVAGILKLMEPCDHILEIQFPLRRDSGDYEMI 509
           FF    S    KLV+DL+++   E+K+ +V GIL++++PC+H+L + FP+RRD G +E+I
Sbjct: 73  FFDRGASIVEDKLVKDLRTQESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVI 132

Query: 510 *AI-AHNIPHTGLQPKEVSILNGRNQR*S*GVSALMTFKCACVDV-LSAVLSRYQDHPKN 683
               A +  H       +      +      +++LMT+KCA VDV      +  + +PKN
Sbjct: 133 EGYRAQHSQHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKN 192



 Score = 45.6 bits (103), Expect = 0.001
 Identities = 30/68 (44%), Positives = 39/68 (57%), Gaps = 3/68 (4%)
 Frame = +2

Query: 512 GYRAQHSTHRTPTKGG--FDSQRT*PEMKLRRISFDDLQVRVRGRAFGGAKP-VSRSSQE 682
           GYRAQHS HRTP KGG  + +  +  E+K    S    +  V    FGGAK  V  + + 
Sbjct: 134 GYRAQHSQHRTPCKGGIRYSTDVSVDEVK-ALASLMTYKCAVVDVPFGGAKAGVKINPKN 192

Query: 683 YSEHELEK 706
           Y+E+ELEK
Sbjct: 193 YTENELEK 200


>UniRef50_Q4T019 Cluster: Chromosome undetermined SCAF11390, whole
           genome shotgun sequence; n=3; Euteleostomi|Rep:
           Chromosome undetermined SCAF11390, whole genome shotgun
           sequence - Tetraodon nigroviridis (Green puffer)
          Length = 618

 Score = 70.5 bits (165), Expect = 4e-11
 Identities = 27/60 (45%), Positives = 46/60 (76%)
 Frame = +3

Query: 330 FFPPSLSSCRRKLVEDLKSRTPIEEKKKKVAGILKLMEPCDHILEIQFPLRRDSGDYEMI 509
           FF   +S    KLVEDLK+R   E+K+ +V GIL++++PC+H+L + FP++RD+G++E++
Sbjct: 57  FFDRGVSIVEDKLVEDLKTRESPEQKRNRVRGILRIIKPCNHVLSVSFPIKRDNGEWEVV 116



 Score = 35.9 bits (79), Expect = 0.97
 Identities = 14/16 (87%), Positives = 14/16 (87%)
 Frame = +2

Query: 512 GYRAQHSTHRTPTKGG 559
           GYRAQHS HRTP KGG
Sbjct: 118 GYRAQHSQHRTPCKGG 133


>UniRef50_Q9VCN3 Cluster: CG4434-PA; n=3; Sophophora|Rep: CG4434-PA
           - Drosophila melanogaster (Fruit fly)
          Length = 535

 Score = 49.2 bits (112), Expect = 1e-04
 Identities = 18/40 (45%), Positives = 30/40 (75%)
 Frame = +1

Query: 256 SHEIPDKLKDIPTSANPKFFHMVEYFFHRACQVVEESLLK 375
           +H++P+KLK + T  +P+F  MV Y++H+A Q +E +LLK
Sbjct: 25  AHQVPEKLKKVETDKDPEFSEMVLYYYHKAAQTMEPALLK 64



 Score = 48.4 bits (110), Expect = 2e-04
 Identities = 27/82 (32%), Positives = 45/82 (54%), Gaps = 1/82 (1%)
 Frame = +3

Query: 399 EEKKKKVAGILKLMEPCDHILEIQFPLRRDSGDYEMI*AI-AHNIPHTGLQPKEVSILNG 575
           EE++ +V  IL L+      +E+ FP+ R +G YE+I    +H++ H       +     
Sbjct: 75  EERQARVTAILNLLGSVSTSVEVNFPIVRKNGTYEIISGYRSHHVRHRLPLKGGIRYALD 134

Query: 576 RNQR*S*GVSALMTFKCACVDV 641
            N+     ++A+MTFKCACV+V
Sbjct: 135 VNESEVKALAAIMTFKCACVNV 156


>UniRef50_Q2S0C1 Cluster: Glutamate dehydrogenase, short peptide;
           n=9; Bacteria|Rep: Glutamate dehydrogenase, short
           peptide - Salinibacter ruber (strain DSM 13855)
          Length = 553

 Score = 42.7 bits (96), Expect = 0.008
 Identities = 25/75 (33%), Positives = 39/75 (52%), Gaps = 2/75 (2%)
 Frame = +3

Query: 423 GILKLMEPCDHILEIQFPLRRDSGDYEMI*AIAHNIPHTGLQPKE--VSILNGRNQR*S* 596
           G+L  +  CD+I+  +FP+ RD G  ++I        H  +QP +  +      N     
Sbjct: 107 GVLHQIRACDNIIRFEFPIERDDGSIQVIRGYRGEHSH-HMQPTKGGIRYAPSVNVDEVM 165

Query: 597 GVSALMTFKCACVDV 641
            +SALM++KCA VDV
Sbjct: 166 ALSALMSYKCAIVDV 180


>UniRef50_Q54KB7 Cluster: Glutamate dehydrogenase, NAD(P)+; n=1;
           Dictyostelium discoideum AX4|Rep: Glutamate
           dehydrogenase, NAD(P)+ - Dictyostelium discoideum AX4
          Length = 502

 Score = 38.7 bits (86), Expect = 0.14
 Identities = 22/74 (29%), Positives = 39/74 (52%), Gaps = 1/74 (1%)
 Frame = +3

Query: 423 GILKLMEPCDHILEIQFPLRRDSGDYEMI*AIAHNIPHTGLQPK-EVSILNGRNQR*S*G 599
           G+L  M+ C+  L ++FP++ + GD ++I        H  L  K  +      + +    
Sbjct: 59  GVLNNMKECNVALRVEFPIKNEHGDVDIIAGYRAQHSHHRLPCKGGIRFSEEVDLQEVMA 118

Query: 600 VSALMTFKCACVDV 641
           +++LMT+KCA VDV
Sbjct: 119 LASLMTYKCAVVDV 132



 Score = 35.1 bits (77), Expect = 1.7
 Identities = 25/67 (37%), Positives = 33/67 (49%), Gaps = 2/67 (2%)
 Frame = +2

Query: 512 GYRAQHSTHRTPTKGGFD-SQRT*PEMKLRRISFDDLQVRVRGRAFGGAK-PVSRSSQEY 685
           GYRAQHS HR P KGG   S+    +  +   S    +  V    FGGAK  V    ++Y
Sbjct: 89  GYRAQHSHHRLPCKGGIRFSEEVDLQEVMALASLMTYKCAVVDVPFGGAKGGVRIDPKKY 148

Query: 686 SEHELEK 706
           +  + EK
Sbjct: 149 TVAQREK 155


>UniRef50_Q24BW7 Cluster: Glutamate/Leucine/Phenylalanine/Valine
           dehydrogenase family protein; n=2;
           Intramacronucleata|Rep:
           Glutamate/Leucine/Phenylalanine/Valine dehydrogenase
           family protein - Tetrahymena thermophila SB210
          Length = 606

 Score = 37.5 bits (83), Expect = 0.32
 Identities = 23/65 (35%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
 Frame = +2

Query: 515 YRAQHSTHRTPTKGGFDSQRT*PEMKLRRIS-FDDLQVRVRGRAFGGAK-PVSRSSQEYS 688
           YRAQH  HR PTKGG    +     ++  +S    L+  V    +GGAK  +  + ++YS
Sbjct: 161 YRAQHKLHRLPTKGGTRYAKDINIQEVEALSCLMTLKCAVVNLPYGGAKGGIGFNPKQYS 220

Query: 689 EHELE 703
             E+E
Sbjct: 221 AREIE 225


>UniRef50_Q23ZD8 Cluster: Glutamate/Leucine/Phenylalanine/Valine
           dehydrogenase family protein; n=1; Tetrahymena
           thermophila SB210|Rep:
           Glutamate/Leucine/Phenylalanine/Valine dehydrogenase
           family protein - Tetrahymena thermophila SB210
          Length = 500

 Score = 37.5 bits (83), Expect = 0.32
 Identities = 23/66 (34%), Positives = 38/66 (57%), Gaps = 3/66 (4%)
 Frame = +2

Query: 515 YRAQHSTHRTPTKGGF--DSQRT*PEMKLRRISFDDLQVRVRGRAFGGAK-PVSRSSQEY 685
           YR QH TH  PTKGGF  + Q +  +++   +  + ++       +GGAK  +  + +EY
Sbjct: 86  YRVQHKTHCLPTKGGFIINDQVSREDIQSFAV-LNTVRSTTLDLPYGGAKGAICINPKEY 144

Query: 686 SEHELE 703
           +E+ELE
Sbjct: 145 TENELE 150


>UniRef50_Q94IH8 Cluster: Glutamate dhydrogenase; n=5;
           Viridiplantae|Rep: Glutamate dhydrogenase - Ulva pertusa
           (Sea lettuce)
          Length = 447

 Score = 36.7 bits (81), Expect = 0.55
 Identities = 23/68 (33%), Positives = 34/68 (50%), Gaps = 2/68 (2%)
 Frame = +2

Query: 509 LGYRAQHSTHRTPTKGGFDSQRT*PEMKLRRI-SFDDLQVRVRGRAFGGAK-PVSRSSQE 682
           +GYR QH   R P KGG    +      +R + S    +  +    FGGAK  ++  ++ 
Sbjct: 86  MGYRVQHDNARGPFKGGLRFHKDADLDDVRSLASLMSFKTALLDVPFGGAKGGITVDTKA 145

Query: 683 YSEHELEK 706
            SEHE+EK
Sbjct: 146 LSEHEIEK 153


>UniRef50_O96248 Cluster: Putative uncharacterized protein PFB0775w;
           n=1; Plasmodium falciparum 3D7|Rep: Putative
           uncharacterized protein PFB0775w - Plasmodium falciparum
           (isolate 3D7)
          Length = 549

 Score = 35.1 bits (77), Expect = 1.7
 Identities = 13/45 (28%), Positives = 24/45 (53%)
 Frame = -3

Query: 569 ENRNLLWLESCVWNVVRDSLNHFVIARIAPQRKLNLKNVIAWFHK 435
           ++ N++W   C + ++ D LN   I+ I+  R   LK +  W+ K
Sbjct: 158 KSTNIIWKRFCFYKLLSDKLNDLHISTISSYRHTYLKTIYIWYKK 202


>UniRef50_Q0W8B3 Cluster: Glutamate dehydrogenase; n=2; uncultured
           methanogenic archaeon RC-I|Rep: Glutamate dehydrogenase
           - Uncultured methanogenic archaeon RC-I
          Length = 439

 Score = 34.7 bits (76), Expect = 2.2
 Identities = 22/67 (32%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
 Frame = +2

Query: 512 GYRAQHSTHRTPTKGGFDSQRT*PEMKLRRIS-FDDLQVRVRGRAFGGAK-PVSRSSQEY 685
           GYR+QH+  R P KGG        E ++  +S    L+  V G  +GGAK  +    ++ 
Sbjct: 56  GYRSQHNNARGPVKGGIRVAPDVTENEVTALSMLMSLKCAVLGLPYGGAKGGIIADPKKL 115

Query: 686 SEHELEK 706
           S+ E+E+
Sbjct: 116 SKAEMER 122


>UniRef50_Q73P03 Cluster: Glutamate dehydrogenase; n=3;
           Bacteria|Rep: Glutamate dehydrogenase - Treponema
           denticola
          Length = 413

 Score = 33.9 bits (74), Expect = 3.9
 Identities = 24/67 (35%), Positives = 33/67 (49%), Gaps = 2/67 (2%)
 Frame = +2

Query: 512 GYRAQHSTHRTPTKGGFDSQRT*PEMKLRRIS-FDDLQVRVRGRAFGGAK-PVSRSSQEY 685
           GYR QHST R P KGG    +     ++R +S +   +  V    +GG K  +  +    
Sbjct: 54  GYRVQHSTLRGPAKGGIRFHQDVNIDEVRSLSAWMTFKCAVADIPYGGGKGGICVNPSNL 113

Query: 686 SEHELEK 706
           SE ELEK
Sbjct: 114 SETELEK 120


>UniRef50_A6DTG1 Cluster: Glutamate dehydrogenase/leucine
           dehydrogenase; n=1; Lentisphaera araneosa HTCC2155|Rep:
           Glutamate dehydrogenase/leucine dehydrogenase -
           Lentisphaera araneosa HTCC2155
          Length = 417

 Score = 33.9 bits (74), Expect = 3.9
 Identities = 25/70 (35%), Positives = 36/70 (51%), Gaps = 5/70 (7%)
 Frame = +2

Query: 512 GYRAQHSTHRTPTKGGFDSQRT*PEMKLRRIS----FDDLQVRVRGRAFGGAK-PVSRSS 676
           GYR QH+    P KGGF   R  P++ L  +        L+  + G  FGGAK  V  + 
Sbjct: 54  GYRIQHNNILGPYKGGF---RYHPQVNLDEVKGLAMLMTLKCSLAGLPFGGAKGGVKFNP 110

Query: 677 QEYSEHELEK 706
           +++S  E+EK
Sbjct: 111 KDFSISEIEK 120


>UniRef50_Q9Y4B6 Cluster: Protein VPRBP; n=26; Fungi/Metazoa
           group|Rep: Protein VPRBP - Homo sapiens (Human)
          Length = 1507

 Score = 33.9 bits (74), Expect = 3.9
 Identities = 23/82 (28%), Positives = 39/82 (47%), Gaps = 8/82 (9%)
 Frame = -2

Query: 237 TFTPAGMIRNIVLRASF*TEFLS-GGTTDLAIFLRCSMVA-------YYVKYENRVRNRV 82
           TF     + +++L   F TEF++ GG   L    R SM A       YY+ Y      RV
Sbjct: 371 TFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERV 430

Query: 81  ALHLLNF*NKLIIFVSFMLDCS 16
            +H  N  + ++ +  ++++CS
Sbjct: 431 CMHPHNVLSDVVNYTLWLMECS 452


>UniRef50_A0BLL2 Cluster: Chromosome undetermined scaffold_114,
           whole genome shotgun sequence; n=6; Paramecium
           tetraurelia|Rep: Chromosome undetermined scaffold_114,
           whole genome shotgun sequence - Paramecium tetraurelia
          Length = 566

 Score = 33.1 bits (72), Expect = 6.8
 Identities = 21/65 (32%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
 Frame = +2

Query: 515 YRAQHSTHRTPTKGGFDSQRT*PEMKLRRIS-FDDLQVRVRGRAFGGAK-PVSRSSQEYS 688
           +RAQH TH+ PTKGG          ++  +S     +  V    +GGAK  +  + ++YS
Sbjct: 82  FRAQHKTHKLPTKGGTRLSEHIHTEEVEALSLLMTFKNAVLELPYGGAKGGLKINPKKYS 141

Query: 689 EHELE 703
           + E+E
Sbjct: 142 KREIE 146


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 729,050,070
Number of Sequences: 1657284
Number of extensions: 14737097
Number of successful extensions: 33900
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 32909
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33873
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 56198352344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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