Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20086X
(334 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000DB7B98 Cluster: PREDICTED: similar to asparagine... 43 0.002
UniRef50_P08243 Cluster: Asparagine synthetase [glutamine-hydrol... 42 0.003
UniRef50_Q4QIC9 Cluster: Putative uncharacterized protein; n=1; ... 34 0.55
UniRef50_A6LX95 Cluster: Carbohydrate-binding family V/XII; n=1;... 34 0.72
UniRef50_UPI0000E2256D Cluster: PREDICTED: similar to synaptotag... 33 0.96
UniRef50_A7DSW5 Cluster: Glutamine amidotransferase, class-II; n... 33 1.3
UniRef50_Q7QTU1 Cluster: GLP_191_3328_5502; n=11; Giardia intest... 32 2.2
UniRef50_A1SKH3 Cluster: ABC transporter related; n=4; Bacteria|... 31 3.9
UniRef50_A0YEF5 Cluster: Asparagine synthase; n=1; marine gamma ... 31 3.9
UniRef50_Q849N4 Cluster: Putative large ATP-binding protein; n=1... 31 6.7
UniRef50_Q83WE9 Cluster: Protomycinolide IV synthase 2; n=1; Mic... 31 6.7
UniRef50_A3RU68 Cluster: SfuB; n=16; Proteobacteria|Rep: SfuB - ... 31 6.7
UniRef50_Q5SFB3 Cluster: Polyketide synthase subunit; n=2; Strep... 30 8.9
UniRef50_A1ASZ8 Cluster: Asparagine synthase; n=2; Desulfuromona... 30 8.9
UniRef50_O16924 Cluster: Asparaginyl trna synthetase protein 2; ... 30 8.9
UniRef50_Q08311 Cluster: Spore coat protein Y; n=5; Bacillus|Rep... 30 8.9
>UniRef50_UPI0000DB7B98 Cluster: PREDICTED: similar to asparagine
synthetase; n=1; Apis mellifera|Rep: PREDICTED: similar
to asparagine synthetase - Apis mellifera
Length = 528
Score = 42.7 bits (96), Expect = 0.002
Identities = 20/39 (51%), Positives = 25/39 (64%), Gaps = 1/39 (2%)
Frame = +3
Query: 108 MCGIWATFGV-TGGLTPTCVKCFSNIRHRGPDAWRIEQD 221
MCGIWA FG+ LT C F I HRGP+A+++E D
Sbjct: 1 MCGIWALFGLNVSSLTNICSN-FEKITHRGPEAFKLEFD 38
Score = 42.3 bits (95), Expect = 0.002
Identities = 19/33 (57%), Positives = 21/33 (63%)
Frame = +2
Query: 236 GYSRIASLAIGDGRHGMQPMRLHCYPRTTLICN 334
GY LAI D +GMQPMRLH YP L+CN
Sbjct: 44 GYFGFHRLAIVDNLYGMQPMRLHQYPHLFLLCN 76
>UniRef50_P08243 Cluster: Asparagine synthetase
[glutamine-hydrolyzing]; n=35; Eumetazoa|Rep: Asparagine
synthetase [glutamine-hydrolyzing] - Homo sapiens
(Human)
Length = 561
Score = 41.9 bits (94), Expect = 0.003
Identities = 19/36 (52%), Positives = 22/36 (61%)
Frame = +3
Query: 108 MCGIWATFGVTGGLTPTCVKCFSNIRHRGPDAWRIE 215
MCGIWA FG L+ C+ I HRGPDA+R E
Sbjct: 1 MCGIWALFGSDDCLSVQCLSAM-KIAHRGPDAFRFE 35
>UniRef50_Q4QIC9 Cluster: Putative uncharacterized protein; n=1;
Leishmania major|Rep: Putative uncharacterized protein -
Leishmania major
Length = 1188
Score = 34.3 bits (75), Expect = 0.55
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = +3
Query: 69 IVSVTVRFYIKDSMCGIWATFGVTGGLTPTCVKCFSNIRHRGPDAWRIEQ 218
+V V FY+ D + W TFG TCVK ++ H WR+ +
Sbjct: 908 VVDREVDFYLTDRLLA-WRTFGAACVAVATCVKELADALHESLRCWRVRE 956
>UniRef50_A6LX95 Cluster: Carbohydrate-binding family V/XII; n=1;
Clostridium beijerinckii NCIMB 8052|Rep:
Carbohydrate-binding family V/XII - Clostridium
beijerinckii NCIMB 8052
Length = 369
Score = 33.9 bits (74), Expect = 0.72
Identities = 18/53 (33%), Positives = 28/53 (52%)
Frame = -1
Query: 274 TVPDSETCNPRIANGSLASCSIRHASGPRWRMFEKHFTQVGVRPPVTPNVAQI 116
TVPDSE+ N +AN + + I +G ++ + H + VG P P + QI
Sbjct: 316 TVPDSESNNAWVANHAYKAGDIVTYNGGTYKCIQAHTSLVGWEPSAVPALWQI 368
>UniRef50_UPI0000E2256D Cluster: PREDICTED: similar to synaptotagmin
XV-a, partial; n=1; Pan troglodytes|Rep: PREDICTED:
similar to synaptotagmin XV-a, partial - Pan troglodytes
Length = 217
Score = 33.5 bits (73), Expect = 0.96
Identities = 22/59 (37%), Positives = 31/59 (52%), Gaps = 1/59 (1%)
Frame = +3
Query: 66 EIVSVTVRFYIKDSMCGIWA-TFGVTGGLTPTCVKCFSNIRHRGPDAWRIEQDAREPLA 239
E+ + ++ + SM G A T G++GG PTC C + H G DA + Q A E LA
Sbjct: 42 ELDTASLSLTVVQSMEGDKAQTAGLSGGGPPTCTPCGRELEHLGRDAQQ-AQGAGEALA 99
>UniRef50_A7DSW5 Cluster: Glutamine amidotransferase, class-II; n=1;
Candidatus Nitrosopumilus maritimus SCM1|Rep: Glutamine
amidotransferase, class-II - Candidatus Nitrosopumilus
maritimus SCM1
Length = 489
Score = 33.1 bits (72), Expect = 1.3
Identities = 14/48 (29%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +3
Query: 111 CGIWATFGVTGG-LTPTCVKCFSNIRHRGPDAWRIEQDAREPLAILGL 251
CG+ + ++G + P + ++HRG +AW + + PL LGL
Sbjct: 16 CGVIGIYSLSGANVIPMAIDALRALQHRGQEAWGLAIPNKPPLKRLGL 63
>UniRef50_Q7QTU1 Cluster: GLP_191_3328_5502; n=11; Giardia
intestinalis|Rep: GLP_191_3328_5502 - Giardia lamblia
ATCC 50803
Length = 724
Score = 32.3 bits (70), Expect = 2.2
Identities = 20/57 (35%), Positives = 24/57 (42%)
Frame = +3
Query: 114 GIWATFGVTGGLTPTCVKCFSNIRHRGPDAWRIEQDAREPLAILGLQVSLSGTVGTV 284
GI GLTPTC C SN R G + + + +P A G SG G V
Sbjct: 403 GIEGCSQCNSGLTPTCTDCKSNYRKEGTNPVKCTKACEDPTACGG----TSGACGAV 455
>UniRef50_A1SKH3 Cluster: ABC transporter related; n=4; Bacteria|Rep:
ABC transporter related - Nocardioides sp. (strain
BAA-499 / JS614)
Length = 1185
Score = 31.5 bits (68), Expect = 3.9
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = -1
Query: 211 IRHASGPRWRMFEKHFTQVGVRPPVTPNVAQIPHMLS 101
I HA G RW E Q +RP +VAQ+P +LS
Sbjct: 1013 IEHAGGLRWNGREVTDPQSFLRPAQVAHVAQVPRVLS 1049
>UniRef50_A0YEF5 Cluster: Asparagine synthase; n=1; marine gamma
proteobacterium HTCC2143|Rep: Asparagine synthase -
marine gamma proteobacterium HTCC2143
Length = 638
Score = 31.5 bits (68), Expect = 3.9
Identities = 14/32 (43%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = +3
Query: 108 MCGIWATFGVTGGLTPTCV-KCFSNIRHRGPD 200
MCGI+A G+ +CV + S++ HRGPD
Sbjct: 1 MCGIFAAM-TRNGIASSCVDRALSSLNHRGPD 31
>UniRef50_Q849N4 Cluster: Putative large ATP-binding protein; n=1;
Streptomyces violaceoruber|Rep: Putative large
ATP-binding protein - Streptomyces violaceoruber
Length = 668
Score = 30.7 bits (66), Expect = 6.7
Identities = 14/43 (32%), Positives = 26/43 (60%)
Frame = -1
Query: 154 GVRPPVTPNVAQIPHMLSLM*NRTVTETISIVNNF*FQTLNSK 26
G PP TP V+ +P +LS + ++ V T+ ++ F T++S+
Sbjct: 495 GTAPPATPTVSAVPDVLSAV-HQLVEATVKASADYLFPTMDSE 536
>UniRef50_Q83WE9 Cluster: Protomycinolide IV synthase 2; n=1;
Micromonospora griseorubida|Rep: Protomycinolide IV
synthase 2 - Micromonospora griseorubida
Length = 1843
Score = 30.7 bits (66), Expect = 6.7
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +3
Query: 210 IEQDAREPLAILGLQVSLSGTVGT 281
+EQ AREP+AI+G+ L G V T
Sbjct: 29 LEQQAREPIAIVGMSCRLPGGVST 52
>UniRef50_A3RU68 Cluster: SfuB; n=16; Proteobacteria|Rep: SfuB -
Ralstonia solanacearum UW551
Length = 603
Score = 30.7 bits (66), Expect = 6.7
Identities = 18/48 (37%), Positives = 21/48 (43%), Gaps = 3/48 (6%)
Frame = +3
Query: 132 GVTGG-LTPTCVKCFSNIRHRGPDAWR--IEQDAREPLAILGLQVSLS 266
GV GG L C C HR PD W ++ P AI GL L+
Sbjct: 385 GVLGGALAVACYTCIGLAMHRKPDGWTRFLDYSVLVPRAIPGLLAGLA 432
>UniRef50_Q5SFB3 Cluster: Polyketide synthase subunit; n=2;
Streptomyces|Rep: Polyketide synthase subunit -
Streptomyces bikiniensis
Length = 1974
Score = 30.3 bits (65), Expect = 8.9
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +3
Query: 210 IEQDAREPLAILGLQVSLSGTVGT 281
+E DAREP+AI+G+ L G V T
Sbjct: 29 LEHDAREPIAIVGMSCRLPGGVTT 52
>UniRef50_A1ASZ8 Cluster: Asparagine synthase; n=2;
Desulfuromonadales|Rep: Asparagine synthase - Pelobacter
propionicus (strain DSM 2379)
Length = 678
Score = 30.3 bits (65), Expect = 8.9
Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 3/60 (5%)
Frame = +3
Query: 108 MCGIWATFGVTGGLTPTCVKC---FSNIRHRGPDAWRIEQDAREPLAILGLQVSLSGTVG 278
MCGI F G +PT + S + HRGPD + D R +A+ +++S+ G G
Sbjct: 1 MCGIAGIFHFEGETSPTLEQVSTMISPLAHRGPDEAGVYLDGR--IALGNVRLSIIGIDG 58
>UniRef50_O16924 Cluster: Asparaginyl trna synthetase protein 2;
n=2; Caenorhabditis|Rep: Asparaginyl trna synthetase
protein 2 - Caenorhabditis elegans
Length = 551
Score = 30.3 bits (65), Expect = 8.9
Identities = 16/37 (43%), Positives = 19/37 (51%)
Frame = +3
Query: 108 MCGIWATFGVTGGLTPTCVKCFSNIRHRGPDAWRIEQ 218
MCGIWA G +TP F I RGPD +E+
Sbjct: 1 MCGIWAIIGEQ--VTPQQQDAFMKIVGRGPDLTVLEE 35
>UniRef50_Q08311 Cluster: Spore coat protein Y; n=5; Bacillus|Rep:
Spore coat protein Y - Bacillus subtilis
Length = 162
Score = 30.3 bits (65), Expect = 8.9
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 2/74 (2%)
Frame = +3
Query: 81 TVRFYIKDSMCGIWATFGVTGGLTPTCVKCFSNIRHRGPDAWRIEQ--DAREPLAILGLQ 254
T+ F + D G+++TFG GG ++CF +I +R+E+ D L+IL
Sbjct: 50 TIPFLVFDKKGGLFSTFGNVGGFVDD-MQCFESI------FFRVEKLCDCCATLSIL-RP 101
Query: 255 VSLSGTVGTVCNRC 296
V + G +VC+ C
Sbjct: 102 VDVKGDTLSVCHPC 115
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 330,288,256
Number of Sequences: 1657284
Number of extensions: 5970872
Number of successful extensions: 15207
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 14900
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15205
length of database: 575,637,011
effective HSP length: 86
effective length of database: 433,110,587
effective search space used: 10394654088
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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