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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20131
         (822 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb...    28   1.8  
SPAC3G9.09c |tif211||translation initiation factor eIF2 alpha su...    27   3.2  
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    25   9.8  

>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 2685

 Score = 27.9 bits (59), Expect = 1.8
 Identities = 14/47 (29%), Positives = 24/47 (51%)
 Frame = +2

Query: 503  IRETVHRLINQLMAHNVPALVHYSLLNRFV*SVSECSMIRRWLYVLS 643
            + E+VH   N+ +AHNV    +  + N  +  V E   +R + Y +S
Sbjct: 1832 LSESVHAFNNRFVAHNVQVKWNNFIRNAVMSYVHEVERVRGFAYYMS 1878


>SPAC3G9.09c |tif211||translation initiation factor eIF2 alpha
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 306

 Score = 27.1 bits (57), Expect = 3.2
 Identities = 10/28 (35%), Positives = 18/28 (64%)
 Frame = -1

Query: 750 ERHARAGRGSALHLIQVEPHKGFILFSK 667
           ++H R GR   + +++V+  KG+I  SK
Sbjct: 60  QKHIRVGRNEVVVVLRVDKEKGYIDLSK 87


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 25.4 bits (53), Expect = 9.8
 Identities = 7/31 (22%), Positives = 19/31 (61%)
 Frame = +2

Query: 281  EKKMFTICSKSKHKSWVKNIRIETIFIWRIR 373
            +KK+ T+  + K + W+ + R   I+++ ++
Sbjct: 1546 QKKLVTLAEQDKKRVWICSFRTNEIYLYGLK 1576


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,582,769
Number of Sequences: 5004
Number of extensions: 76914
Number of successful extensions: 224
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 402440190
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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