Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20056
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 168 8e-43
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 109 3e-25
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 109 3e-25
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 99 8e-22
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 92 7e-20
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 49 6e-07
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 40 5e-04
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 31 0.23
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.94
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 27 2.2
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 27 2.9
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 26 5.0
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 8.7
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 8.7
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 168 bits (408), Expect = 8e-43
Identities = 97/222 (43%), Positives = 129/222 (58%), Gaps = 2/222 (0%)
Frame = +3
Query: 90 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTP-TT 266
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ P T
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Query: 267 QYSMPNVSSDVSSKMLLCKPT*STGLSSCQ*WRQT*DQGSI*G*RQNLFPRGSQFHGAYE 446
+ + + + + K S + + ++ G ++ P +
Sbjct: 98 IFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSK 157
Query: 447 NEGNCRSLSWQNCAECSYHGSAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 626
+ + + AYFND+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLD
Sbjct: 158 MKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLD 217
Query: 627 KKGTGERNVLIFDLGGGTSTCPSLP-SRRYLRGEIHRGDTHL 749
K T E++++++DLGGGT L GDTHL
Sbjct: 218 KTDT-EKHIVVYDLGGGTFDVSLLSIDNGVFEVLATSGDTHL 258
Score = 71.7 bits (168), Expect = 1e-13
Identities = 31/60 (51%), Positives = 46/60 (76%)
Frame = +1
Query: 331 EALAFRVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 510
++ F +V+D +P ++V G+ K F PEE+S+M+L+KMK+TAEAYLGK V +AV+TVP
Sbjct: 119 KSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVP 178
Score = 48.4 bits (110), Expect = 1e-06
Identities = 23/57 (40%), Positives = 35/57 (61%)
Frame = +2
Query: 254 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFELSVMEANLRSR*HIRVKTKPFSPRK 424
+P NTIFD KRLIGRKF++ T+ D+K +PF + + ++ K K F+P +
Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEE 149
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 109 bits (263), Expect = 3e-25
Identities = 50/58 (86%), Positives = 54/58 (93%)
Frame = +3
Query: 87 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTP 260
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
Score = 104 bits (250), Expect = 1e-23
Identities = 55/81 (67%), Positives = 58/81 (71%), Gaps = 1/81 (1%)
Frame = +3
Query: 510 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTSTC 689
AYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ E NVLIFDLGGGT
Sbjct: 146 AYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFDV 205
Query: 690 PSLP-SRRYLRGEIHRGDTHL 749
L + GDTHL
Sbjct: 206 SLLTIEEGIFEVKATAGDTHL 226
Score = 77.0 bits (181), Expect = 3e-15
Identities = 35/56 (62%), Positives = 45/56 (80%)
Frame = +1
Query: 343 FRVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 510
F+V+ GKP I+V +KGE KTF PEE+SSMVL KM+E+AEA+LG V +AV+TVP
Sbjct: 90 FKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVTDAVVTVP 145
Score = 65.3 bits (152), Expect = 9e-12
Identities = 28/57 (49%), Positives = 40/57 (70%)
Frame = +2
Query: 254 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFELSVMEANLRSR*HIRVKTKPFSPRK 424
+P+NTIFDAKRLIGR+F D VQ+DMKHWPF++ + + + +TK F+P +
Sbjct: 60 NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEE 116
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 109 bits (263), Expect = 3e-25
Identities = 50/58 (86%), Positives = 54/58 (93%)
Frame = +3
Query: 87 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTP 260
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
Score = 107 bits (258), Expect = 1e-24
Identities = 56/81 (69%), Positives = 59/81 (72%), Gaps = 1/81 (1%)
Frame = +3
Query: 510 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTSTC 689
AYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE NVLIFDLGGGT
Sbjct: 146 AYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFDV 205
Query: 690 PSLP-SRRYLRGEIHRGDTHL 749
L + GDTHL
Sbjct: 206 SLLTIEEGIFEVKATAGDTHL 226
Score = 82.6 bits (195), Expect = 5e-17
Identities = 38/56 (67%), Positives = 46/56 (82%)
Frame = +1
Query: 343 FRVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 510
F+V+S GKP ++V YKGE KTF PEE+SSMVL KM+ETAEAYLG V +AV+TVP
Sbjct: 90 FKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVP 145
Score = 66.5 bits (155), Expect = 4e-12
Identities = 29/57 (50%), Positives = 41/57 (71%)
Frame = +2
Query: 254 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFELSVMEANLRSR*HIRVKTKPFSPRK 424
+P+NTIFDAKRLIGRKF+D VQ+DMKHWPF++ + + + +TK F+P +
Sbjct: 60 NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEE 116
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 98.7 bits (235), Expect = 8e-22
Identities = 53/81 (65%), Positives = 59/81 (72%), Gaps = 1/81 (1%)
Frame = +3
Query: 510 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTSTC 689
AYF+DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K +NVLIFDLGGGT
Sbjct: 150 AYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGTFDV 209
Query: 690 PSLPSRRYLRGEI-HRGDTHL 749
L + + + GDTHL
Sbjct: 210 SLLKIQGGVFEVLATAGDTHL 230
Score = 97.9 bits (233), Expect = 1e-21
Identities = 44/58 (75%), Positives = 50/58 (86%)
Frame = +3
Query: 87 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTP 260
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AM P
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65
Score = 66.9 bits (156), Expect = 3e-12
Identities = 32/56 (57%), Positives = 41/56 (73%)
Frame = +1
Query: 343 FRVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 510
F+V+ + G P I+V Y GE K F +E+S+MVLTKMKE +EA L K V+ AVITVP
Sbjct: 94 FKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVP 149
Score = 56.8 bits (131), Expect = 3e-09
Identities = 21/33 (63%), Positives = 28/33 (84%)
Frame = +2
Query: 254 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFEL 352
+P NT+FDAKRLIGR++ED Q D+KHWPF++
Sbjct: 64 NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKV 96
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 92.3 bits (219), Expect = 7e-20
Identities = 49/81 (60%), Positives = 56/81 (69%), Gaps = 1/81 (1%)
Frame = +3
Query: 510 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTSTC 689
AYFNDSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK + V +FDLGGGT
Sbjct: 192 AYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK--NDAIVAVFDLGGGTFDI 249
Query: 690 PSLP-SRRYLRGEIHRGDTHL 749
L + GDTHL
Sbjct: 250 SILELNNGVFEVRSTNGDTHL 270
Score = 70.9 bits (166), Expect = 2e-13
Identities = 33/66 (50%), Positives = 44/66 (66%), Gaps = 1/66 (1%)
Frame = +3
Query: 78 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 254
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q +
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 255 TPTTQY 272
P +
Sbjct: 109 NPENTF 114
Score = 50.8 bits (116), Expect = 2e-07
Identities = 20/36 (55%), Positives = 28/36 (77%)
Frame = +1
Query: 403 KTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 510
KT+ P ++ +L+KM+ETA YLGK V+NAV+TVP
Sbjct: 156 KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVP 191
Score = 40.7 bits (91), Expect = 2e-04
Identities = 16/33 (48%), Positives = 24/33 (72%)
Frame = +2
Query: 254 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFEL 352
+P NT F KRLIGR+F++ VQ D+K P+++
Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKI 141
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 49.2 bits (112), Expect = 6e-07
Identities = 20/59 (33%), Positives = 38/59 (64%)
Frame = +3
Query: 75 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 251
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61
Score = 49.2 bits (112), Expect = 6e-07
Identities = 30/78 (38%), Positives = 42/78 (53%), Gaps = 4/78 (5%)
Frame = +3
Query: 510 AYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLGGG 677
A+F D QR+A +A I+GLN LR++N+ AAA+ YG+ K E R V I D G
Sbjct: 150 AWFTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHS 209
Query: 678 TSTCPSLPSRRYLRGEIH 731
S+ + RG+ H
Sbjct: 210 NY---SVSIVEFSRGQFH 224
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 39.5 bits (88), Expect = 5e-04
Identities = 16/53 (30%), Positives = 30/53 (56%)
Frame = +3
Query: 90 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQV 248
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQL 79
Score = 39.1 bits (87), Expect = 7e-04
Identities = 19/64 (29%), Positives = 35/64 (54%)
Frame = +3
Query: 492 CSYHGSAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLG 671
C YF D+QR+A + A +GL VL++I++P A +A ++ ++ V++ + G
Sbjct: 166 CVMSVPVYFTDAQRKALESAANEAGLPVLQLIHDPAAVILALMYSEEVLIDKTVVVANFG 225
Query: 672 GGTS 683
S
Sbjct: 226 ATRS 229
Score = 27.5 bits (58), Expect = 2.2
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +1
Query: 397 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 510
++K E S L ++ E+AE +LG V V++VP
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVP 171
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 30.7 bits (66), Expect = 0.23
Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
Frame = +3
Query: 90 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTPTT 266
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83
Query: 267 QYSMPNV 287
+S+ NV
Sbjct: 84 -HSIRNV 89
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.94
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 82 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 177
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 27.5 bits (58), Expect = 2.2
Identities = 20/88 (22%), Positives = 37/88 (42%)
Frame = -2
Query: 590 IDDSENVQARDGTCIFCGLSLRVIEVRGTVITAFCTVLPR*ASAVSFIFVSTMELTSSGK 411
I+DSE+V A + + L+ + E T P + ++ +S + + K
Sbjct: 2059 IEDSEDVHANSPSVLIVQLTRELEEDEEVDTTVIAPYFPAQKTEHWWLVISDDKTLLAIK 2118
Query: 410 KVLSSPLYATLILGLPPSLTTRKASASC 327
K+ T + +PP++ T K SC
Sbjct: 2119 KITLGRSLTTKMEFVPPAMGTLKYKLSC 2146
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 27.1 bits (57), Expect = 2.9
Identities = 17/55 (30%), Positives = 27/55 (49%)
Frame = -1
Query: 168 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARLLV 4
G W SP S + H ++++ +F +RVL C+ L K +K+A L V
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALLAV 325
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 26.2 bits (55), Expect = 5.0
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +3
Query: 186 PSYVAFTDTERLIGDAAKNQVAMTPTTQYSMPNVSSDVSSKMLLC 320
P +A T ER++ AAK A+ T Y++ ++ D K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 25.4 bits (53), Expect = 8.7
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +3
Query: 435 GAYENEGNCRSLSWQNCAECSYHGSAYFND 524
GAY N C +S N A CS SA ND
Sbjct: 1689 GAYSNA--CVDISLSNLALCSILNSALIND 1716
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +3
Query: 198 AFTDTERLIGDAAKNQVAMTPTTQYSMPNVSSDV 299
AF D + G AK A P+T+ + N+S V
Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,365,986
Number of Sequences: 5004
Number of extensions: 73578
Number of successful extensions: 268
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 264
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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