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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00069
         (738 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    64   2e-11
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    51   2e-07
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    39   9e-04
SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog, Rhp...    32   0.074
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2...    31   0.17 
SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|ch...    29   0.91 
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    27   2.1  
SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schi...    27   2.8  
SPAC1296.01c ||SPAC22F3.01|phosphoacetylglucosamine mutase |Schi...    27   3.7  
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    26   4.9  
SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c...    26   4.9  
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch...    26   4.9  
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr...    26   6.4  
SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe...    26   6.4  

>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 64.1 bits (149), Expect = 2e-11
 Identities = 30/42 (71%), Positives = 35/42 (83%)
 Frame = +1

Query: 1   VISMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKSQE 126
           VISML+ PNDESPAN+DAAKE+RE   EFKK+V R VR+S E
Sbjct: 124 VISMLSSPNDESPANIDAAKEFRENPQEFKKRVRRLVRRSIE 165


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 50.8 bits (116), Expect = 2e-07
 Identities = 23/40 (57%), Positives = 29/40 (72%)
 Frame = +1

Query: 1   VISMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKS 120
           V+SMLA+PNDES AN+DA K WRE   E+ + V R  RK+
Sbjct: 124 VMSMLAEPNDESGANIDACKMWREDREEYCRVVRRLARKT 163


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 38.7 bits (86), Expect = 9e-04
 Identities = 18/40 (45%), Positives = 26/40 (65%)
 Frame = +1

Query: 7   SMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKSQE 126
           S+L DPN+ SPAN +AA+  RE   E+ ++V + V  S E
Sbjct: 111 SLLNDPNNASPANAEAAQLHRENKKEYVRRVRKTVEDSWE 150


>SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog,
           Rhp9|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 778

 Score = 32.3 bits (70), Expect = 0.074
 Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
 Frame = -2

Query: 461 TTSHQSLSQSKQI-MKKGTVIRNNPLGSFSHTVLLSF*TPNRILSTLSAKMKQN 303
           T S  + +Q K+  +KKG V+++  LG   HTV+ +F + N  LS ++     N
Sbjct: 404 TMSTVNSNQIKRFFLKKGDVVQSTRLGKIKHTVVKTFRSTNEQLSLIAVDALNN 457


>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 176

 Score = 31.1 bits (67), Expect = 0.17
 Identities = 13/40 (32%), Positives = 24/40 (60%)
 Frame = +1

Query: 7   SMLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKSQE 126
           S+L +PN+ SP N  AA+ W +   E+K+ + +  ++  E
Sbjct: 136 SLLGEPNNASPLNAQAAELWSKDPIEYKRLLMQRYKEIDE 175


>SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 548

 Score = 28.7 bits (61), Expect = 0.91
 Identities = 14/41 (34%), Positives = 23/41 (56%)
 Frame = +2

Query: 227 NVIHLASPYDPLTPNPHTSTADVFAYSVSFLQKEWRESYSE 349
           +V+HL  P D LTP PH    D F   + +  + W+ ++S+
Sbjct: 53  SVLHLRGPSDHLTPQPH---VDSFGNVLCWNGEIWQINHSD 90


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 14/36 (38%), Positives = 20/36 (55%)
 Frame = +1

Query: 1   VISMLADPNDESPANVDAAKEWRERYSEFKKKVARC 108
           +IS+L++ +  SP NVDAAK  R         + RC
Sbjct: 111 IISLLSNYDASSPLNVDAAKLLRTGDKTAYNSLVRC 146


>SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase
           Ran1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 470

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 9/21 (42%), Positives = 14/21 (66%)
 Frame = +2

Query: 242 ASPYDPLTPNPHTSTADVFAY 304
           A+ Y P+TP P+ + A +F Y
Sbjct: 415 ANSYMPITPTPYPNNAKIFGY 435


>SPAC1296.01c ||SPAC22F3.01|phosphoacetylglucosamine mutase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 542

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 18/63 (28%), Positives = 29/63 (46%), Gaps = 1/63 (1%)
 Frame = +2

Query: 188 IVTPANI-LLQTLKNVIHLASPYDPLTPNPHTSTADVFAYSVSFLQKEWRESYSEFKRKV 364
           +++PA    L+TLK V  L +  D            + A+    L KEW + YSE   ++
Sbjct: 407 VLSPAQFNALKTLKTVFELINQTDGDAITNLLLVEVILAHKNCTL-KEWNQLYSEIPSRL 465

Query: 365 AQC 373
            +C
Sbjct: 466 IRC 468


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 12/37 (32%), Positives = 22/37 (59%)
 Frame = +1

Query: 10  MLADPNDESPANVDAAKEWRERYSEFKKKVARCVRKS 120
           +L DPN  SPA  +A   +++   E++K+V    R++
Sbjct: 119 LLDDPNIASPAQTEAYTMFKKDKVEYEKRVRAQAREN 155


>SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1717

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = -2

Query: 470 SVLTTSHQSLSQSKQIMKKGTVIRNNPLG 384
           ++ T    S  +SKQ +K+ T ++N+P G
Sbjct: 497 TIFTQGPNSTKESKQAVKEETSVKNSPKG 525


>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
           Eme1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 15/47 (31%), Positives = 22/47 (46%)
 Frame = +2

Query: 2   SFQCSLIQMMRVPLMLMLQKNGENDIRNSKRKLPDVLGKVKKIASRS 142
           S Q    + MR+P +            NS +K  D L K++K+ SRS
Sbjct: 331 SIQSRAFKKMRLPKISRTTDTPPASTSNSNKKNLDKLKKMRKLCSRS 377


>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1471

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 10/22 (45%), Positives = 16/22 (72%)
 Frame = +2

Query: 14   SLIQMMRVPLMLMLQKNGENDI 79
            SL+ +M+ PL+  L+KN  ND+
Sbjct: 1351 SLLPLMQSPLVFCLRKNDANDL 1372


>SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 527

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 18/46 (39%), Positives = 24/46 (52%)
 Frame = -3

Query: 601 KKDEKKTFNSILFIKYGSDTISLL*VFILNQLNIVSGPGGGVRSAS 464
           KKDEKK  N + +I      +SL  +F  N  +IV   G G + AS
Sbjct: 313 KKDEKKALNLLAWILKLYSYMSLFILFGSNYSDIVLLFGAGSKWAS 358


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,183,627
Number of Sequences: 5004
Number of extensions: 68576
Number of successful extensions: 216
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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