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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00062
         (804 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...   148   1e-36
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    86   5e-18
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    79   7e-16
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...    77   4e-15
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    73   4e-14
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2...    70   4e-13
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    69   1e-12
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch...    66   4e-12
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac...    66   6e-12
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac...    60   5e-10
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    59   6e-10
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    55   1e-08
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch...    50   5e-07
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos...    33   0.036
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|...    28   1.8  
SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ...    27   3.1  
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ...    27   3.1  
SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos...    26   7.2  
SPBC32F12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    26   7.2  
SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces po...    25   9.5  

>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score =  148 bits (358), Expect = 1e-36
 Identities = 64/96 (66%), Positives = 76/96 (79%)
 Frame = +1

Query: 223 FCREAYEESRCSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFH 402
           F  +  + S   L+LM W+  IPGK  T WEGGLYKL M F ++YP+ PPKC+F PPLFH
Sbjct: 24  FYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFH 83

Query: 403 PNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDL 510
           PNVYPSGTVCLS+L+EE+ W+PAITIKQILLGIQDL
Sbjct: 84  PNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDL 119



 Score = 51.2 bits (117), Expect = 2e-07
 Identities = 20/33 (60%), Positives = 24/33 (72%)
 Frame = +2

Query: 155 MSGIASARLAEERKAWRKDHPFGFVARPMKNPD 253
           MS +   RL EERK WR+DHPFGF A+P K+ D
Sbjct: 1   MSSLCKTRLQEERKQWRRDHPFGFYAKPCKSSD 33



 Score = 39.1 bits (87), Expect = 7e-04
 Identities = 15/37 (40%), Positives = 26/37 (70%)
 Frame = +3

Query: 480 KANFVGYSRSLNEPNVKDPAQAEAYTIYCQNRLEYDK 590
           K   +G    L++PN+  PAQ EAYT++ ++++EY+K
Sbjct: 110 KQILLGIQDLLDDPNIASPAQTEAYTMFKKDKVEYEK 146


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 86.2 bits (204), Expect = 5e-18
 Identities = 40/94 (42%), Positives = 52/94 (55%), Gaps = 11/94 (11%)
 Frame = +1

Query: 262 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 441
           +  TW+C I G  GTP+EGGLY   + F  DYP  PP  KFE   FHPNVY  GTVC+S+
Sbjct: 34  DFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKFECEFFHPNVYKDGTVCISI 93

Query: 442 LDEEKD-----------WRPAITIKQILLGIQDL 510
           L    D           W P  ++++ILL +  +
Sbjct: 94  LHAPGDDPNMYESSSERWSPVQSVEKILLSVMSM 127


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 79.0 bits (186), Expect = 7e-16
 Identities = 37/88 (42%), Positives = 49/88 (55%)
 Frame = +1

Query: 247 SRCSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGT 426
           S  S N+M W   I G   TP+E G +KL + F + YP+ PP  KF   +FHPNVY +G 
Sbjct: 27  SPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGE 86

Query: 427 VCLSLLDEEKDWRPAITIKQILLGIQDL 510
           +CL +L  +  W P   +  IL  IQ L
Sbjct: 87  LCLDIL--QNRWSPTYDVAAILTSIQSL 112



 Score = 29.5 bits (63), Expect = 0.58
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = +3

Query: 510 LNEPNVKDPAQAEAYTIYCQNRLEY 584
           LN+PN   PA AEA  ++ +N+ EY
Sbjct: 113 LNDPNNASPANAEAAQLHRENKKEY 137


>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score = 76.6 bits (180), Expect = 4e-15
 Identities = 32/83 (38%), Positives = 51/83 (61%)
 Frame = +1

Query: 262 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 441
           +L  W+  I G   +P+ GG++ L + F  DYP  PPK  F   ++HPN+  +G++CL +
Sbjct: 29  DLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDI 88

Query: 442 LDEEKDWRPAITIKQILLGIQDL 510
           L ++  W PA+TI ++LL I  L
Sbjct: 89  LRDQ--WSPALTISKVLLSICSL 109


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 73.3 bits (172), Expect = 4e-14
 Identities = 33/83 (39%), Positives = 50/83 (60%)
 Frame = +1

Query: 262 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 441
           NL  ++  + G + + +EGG + L +   D+YP  PP  +F   ++HPNV   G +CLS 
Sbjct: 30  NLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLST 89

Query: 442 LDEEKDWRPAITIKQILLGIQDL 510
           L  +KDW PA+ I+ +LL IQ L
Sbjct: 90  L--KKDWSPALQIRTVLLSIQAL 110


>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 176

 Score = 69.7 bits (163), Expect = 4e-13
 Identities = 33/83 (39%), Positives = 47/83 (56%)
 Frame = +1

Query: 262 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 441
           NL+ W   I G   T +EG  +K+ M F  +YP SPP   F  P++HPNV  SG +CL +
Sbjct: 57  NLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDI 116

Query: 442 LDEEKDWRPAITIKQILLGIQDL 510
           L ++  W     ++ ILL +Q L
Sbjct: 117 LKDK--WSAVYNVQTILLSLQSL 137


>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 68.5 bits (160), Expect = 1e-12
 Identities = 28/66 (42%), Positives = 40/66 (60%)
 Frame = +1

Query: 262 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 441
           ++  WE  I G + T +EGG +   + F  DYP  PPK KF   ++HPNV+P+G VC+S+
Sbjct: 34  SIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISI 93

Query: 442 LDEEKD 459
           L    D
Sbjct: 94  LHPPGD 99


>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
           Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 66.5 bits (155), Expect = 4e-12
 Identities = 29/74 (39%), Positives = 45/74 (60%), Gaps = 1/74 (1%)
 Frame = +1

Query: 292 GKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRP 468
           G +GTP+EGG + + +    DYP  PPK  F+  ++HPNV   +G +CL +L ++  W P
Sbjct: 42  GPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQ--WSP 99

Query: 469 AITIKQILLGIQDL 510
             T+K  L+ +Q L
Sbjct: 100 VYTMKSALISLQSL 113


>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
           Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 177

 Score = 66.1 bits (154), Expect = 6e-12
 Identities = 28/70 (40%), Positives = 44/70 (62%)
 Frame = +1

Query: 310 WEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQI 489
           ++GG +K R+   D+YP  PPK K    ++HPN+   G VCL++L   +DW P + +  I
Sbjct: 65  YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNIL--RQDWNPVLNLNSI 122

Query: 490 LLGIQDLSMS 519
           L+G+Q L +S
Sbjct: 123 LVGLQFLFLS 132


>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
           Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 59.7 bits (138), Expect = 5e-10
 Identities = 27/83 (32%), Positives = 45/83 (54%)
 Frame = +1

Query: 262 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 441
           NL  W C   G   + + GG +   + F  DYP  PP  +F   ++HPN    G VCL++
Sbjct: 35  NLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAI 94

Query: 442 LDEEKDWRPAITIKQILLGIQDL 510
           L +++ ++P+I ++ +L  I  L
Sbjct: 95  L-KQQVFKPSIKLRSVLEQILQL 116


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 59.3 bits (137), Expect = 6e-10
 Identities = 28/85 (32%), Positives = 47/85 (55%), Gaps = 1/85 (1%)
 Frame = +1

Query: 259 LNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCL 435
           +++  W+  I G   TP+EGG + L +   + YP SPP   F+  + HPN+ + +G VC+
Sbjct: 32  VDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCM 91

Query: 436 SLLDEEKDWRPAITIKQILLGIQDL 510
            +L  +  W PA +++   L I  L
Sbjct: 92  DIL--KTHWSPAWSLQSACLAIISL 114


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 54.8 bits (126), Expect = 1e-08
 Identities = 28/81 (34%), Positives = 39/81 (48%), Gaps = 1/81 (1%)
 Frame = +1

Query: 262 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 441
           N++ W   I G   TP+EGG Y   +IF  DYP  PP  +   P        +  +CLS 
Sbjct: 33  NILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRLCLSF 90

Query: 442 LD-EEKDWRPAITIKQILLGI 501
            D   K W P+  +  IL+G+
Sbjct: 91  SDFHPKSWNPSWMVSTILVGL 111


>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
           Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 49.6 bits (113), Expect = 5e-07
 Identities = 24/78 (30%), Positives = 41/78 (52%), Gaps = 1/78 (1%)
 Frame = +1

Query: 238 YEESRCSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYP 417
           YE +  + N+  +     G   TP+ GG++K+ +    +YP   P   F   +FHPN+  
Sbjct: 20  YEVTLVNDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDE 79

Query: 418 -SGTVCLSLLDEEKDWRP 468
            SG+VCL ++++   W P
Sbjct: 80  LSGSVCLDVINQ--TWSP 95


>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
           Mms2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 139

 Score = 33.5 bits (73), Expect = 0.036
 Identities = 25/97 (25%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
 Frame = +1

Query: 259 LNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCL 435
           + L  W   I G   +  E  +Y L++    +YP +PP   F   +  P V   +G V  
Sbjct: 36  ITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNP 95

Query: 436 SLLDEEKDWRPAITIKQILLGIQDLSMSLT*KIQRKL 546
             +D  + W+   +++ +LL   DL   +     RKL
Sbjct: 96  HKIDCLRHWKREYSMETVLL---DLKKEMASSSNRKL 129


>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 574

 Score = 27.9 bits (59), Expect = 1.8
 Identities = 12/36 (33%), Positives = 22/36 (61%)
 Frame = -1

Query: 213 WSLRQAFRSSAKRALAIPDIFYKNNSIYEPSFNQGL 106
           W LR+  +S++  +    ++F K NSIY  +F++ L
Sbjct: 71  WQLREQEQSNSHISRNENELFSKENSIYNGNFSENL 106


>SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 527

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 18/60 (30%), Positives = 30/60 (50%)
 Frame = -3

Query: 355 NHL*RSYVVYTAHLPMESPFYQVSHIPKSLNSMNIWILHRPRDKTKGMVLTPSFPFFS*T 176
           N + RS   + A LPME+    +SH  ++++       + P  +T  +  TP+F F S T
Sbjct: 370 NTMLRSAFSHVARLPMETVSQLLSHYTETISQ------YLPPSETSPIPKTPNFKFISST 423


>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 509

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 13/38 (34%), Positives = 19/38 (50%)
 Frame = -2

Query: 383 NLHFGGLDG*SSLKIIRSLYSPPSHGVPFLPGIAHSQV 270
           N  F G+     L  + S  SP  HG P +PG+ H+ +
Sbjct: 442 NPQFSGVLNLPGLGTMPSFPSPYQHGQPQIPGMLHASL 479


>SPBC16G5.15c |fkh2||fork head transcription factor Fkh2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 642

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 9/24 (37%), Positives = 15/24 (62%)
 Frame = +1

Query: 259 LNLMTWECAIPGKKGTPWEGGLYK 330
           L+  TW C++ G+ G   +G L+K
Sbjct: 138 LDDQTWNCSVYGRNGIKVDGKLFK 161


>SPBC32F12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 340

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 8/19 (42%), Positives = 12/19 (63%)
 Frame = +1

Query: 220 WFCREAYEESRCSLNLMTW 276
           W C E Y++  CSL+  +W
Sbjct: 15  WICYEEYDKKLCSLSNDSW 33


>SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 472

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 11/24 (45%), Positives = 13/24 (54%)
 Frame = -2

Query: 155 FSTKITRFMSRASIKDYS*CWAID 84
           FS +ITRF  R    D+  CW  D
Sbjct: 343 FSAEITRFADRNFYDDWWNCWTWD 366


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,334,616
Number of Sequences: 5004
Number of extensions: 71883
Number of successful extensions: 188
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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