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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fprWP01_F_E02
         (609 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC576.07 |ret3||coatomer zeta subunit |Schizosaccharomyces pom...   105   7e-24
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac...    41   1e-04
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc...    31   0.100
SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc...    29   0.70 
SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe...    26   3.7  
SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe...    25   6.5  
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ...    25   8.7  
SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb...    25   8.7  

>SPCC576.07 |ret3||coatomer zeta subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 190

 Score =  105 bits (251), Expect = 7e-24
 Identities = 54/146 (36%), Positives = 82/146 (56%), Gaps = 8/146 (5%)
 Frame = +2

Query: 122 TLYIVKGMCILDYEGNRILAKYY--------DKDVLPTTKEQKAFEKNLFNKTHRANAEI 277
           TLY V    ILD  G RI  KYY        +  V  + KE+K FEK LF KT +   +I
Sbjct: 4   TLYAVNAFLILDSSGKRIFTKYYAPPHLKEGEGGVFNSVKEEKTFEKGLFEKTWKTQNDI 63

Query: 278 IMLDGLTCVYKSNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVLMENLD 457
           +  DG   V  + +D+ FY++G   ENE++L   L ++ +++ LL +   ++R L+EN D
Sbjct: 64  LTYDGKLVVMLTVMDVIFYIVGGMEENEVMLYECLRSIRDALELLFKYVPDKRTLLENYD 123

Query: 458 AVMLAFDEICDGGVILDADPTSIVSR 535
            +++  DE  D GVIL+ +P  I +R
Sbjct: 124 QLVIVVDETIDDGVILETEPALIAAR 149


>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 446

 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 26/138 (18%), Positives = 63/138 (45%)
 Frame = +2

Query: 131 IVKGMCILDYEGNRILAKYYDKDVLPTTKEQKAFEKNLFNKTHRANAEIIMLDGLTCVYK 310
           ++ G+ I + +G+ ++ K +  D+  +  E   F   +   T   +  I+ +   T +Y 
Sbjct: 1   MISGLFIFNLKGDTLICKTFRHDLKKSVTE--IFRVAILTNTDYRHP-IVSIGSSTYIYT 57

Query: 311 SNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVLMENLDAVMLAFDEICD 490
            + DL+   +   + N +I+   L +L + ++    +  E  V  +N+  +    DE+ D
Sbjct: 58  KHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTV-KDNVSFIFELLDEMID 116

Query: 491 GGVILDADPTSIVSRAAL 544
            G+I   +P ++    ++
Sbjct: 117 YGIIQTTEPDALARSVSI 134


>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 143

 Score = 31.5 bits (68), Expect = 0.100
 Identities = 28/136 (20%), Positives = 58/136 (42%), Gaps = 1/136 (0%)
 Frame = +2

Query: 131 IVKGMCILDYEGNRILAKYYDK-DVLPTTKEQKAFEKNLFNKTHRANAEIIMLDGLTCVY 307
           +++ + I +  G   L+KYY   D     + +    + +  +  +  A  +  +    VY
Sbjct: 1   MIQFILIQNRHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVY 60

Query: 308 KSNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVLMENLDAVMLAFDEIC 487
           +    L+F     S +N+L +  +++   E +      N+    L+ N   V    DEI 
Sbjct: 61  RRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFF-GNVCELDLIFNFYKVSAILDEII 119

Query: 488 DGGVILDADPTSIVSR 535
            GG I +++  S++ R
Sbjct: 120 LGGEIGESNKKSVLER 135


>SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 162

 Score = 28.7 bits (61), Expect = 0.70
 Identities = 19/81 (23%), Positives = 36/81 (44%)
 Frame = +2

Query: 290 GLTCVYKSNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVLMENLDAVML 469
           G   VY+    LFF       +NELI+  V++   E +      N+    L+ N +    
Sbjct: 55  GEKIVYRRYASLFFVCGIEQDDNELIILEVIHKFVECLDKYF-GNVCELDLIFNFEKAYY 113

Query: 470 AFDEICDGGVILDADPTSIVS 532
             +E+   G + ++  T+++S
Sbjct: 114 VMEELLLAGELQESSKTNVLS 134


>SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 548

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 9/21 (42%), Positives = 13/21 (61%)
 Frame = +3

Query: 423 TWSGGFSWRTWMLLCLPSMRS 485
           +WS G  +  W+ + LPSM S
Sbjct: 437 SWSNGIDYAKWISVALPSMLS 457


>SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 652

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 20/78 (25%), Positives = 34/78 (43%), Gaps = 13/78 (16%)
 Frame = +2

Query: 221 QKAFEKNLFNKTHRANAEIIMLDGLTCVYKS-------------NVDLFFYVMGSSHENE 361
           Q  F  N+  K+H+    ++ L  L  V+K+             N +L F   G +    
Sbjct: 201 QDVFFTNMSMKSHKLGTSLLALHALNHVFKTRDRVLKNSARISQNPELEFRDQGYTRPKV 260

Query: 362 LILQSVLNALYESVSLLL 415
           LIL    N+ +E ++LL+
Sbjct: 261 LILLPTRNSAFEFINLLI 278


>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 639

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 11/44 (25%), Positives = 22/44 (50%)
 Frame = +2

Query: 311 SNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVL 442
           ++V   ++V  +    ELI++ +   LYES  +     + R +L
Sbjct: 87  ADVPAIYFVQPTQENIELIIEDLSKGLYESAYVCFSSTISRALL 130


>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 814

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 10/35 (28%), Positives = 21/35 (60%)
 Frame = +2

Query: 191 DKDVLPTTKEQKAFEKNLFNKTHRANAEIIMLDGL 295
           D+D +P  K + + +KN+ N + +  A+++   GL
Sbjct: 55  DEDDIPIRKRRASSKKNMSNSSSKKRAKVMGNGGL 89


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,473,042
Number of Sequences: 5004
Number of extensions: 47733
Number of successful extensions: 127
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 268287866
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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