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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_F01
         (653 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po...   202   5e-53
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po...   198   4e-52
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy...    31   0.11 
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces...    29   0.44 
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar...    25   7.2  
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi...    25   7.2  
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom...    25   9.5  

>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  202 bits (492), Expect = 5e-53
 Identities = 100/178 (56%), Positives = 126/178 (70%), Gaps = 2/178 (1%)
 Frame = +2

Query: 122 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSW 301
           +  +E+ V  AKLAEQAERY+ M   MK V  T  EL+ EERNLLSVAYKNV+GARR+SW
Sbjct: 3   TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62

Query: 302 RVISSIEQKTE--GSERKQQMAKEYXVKVQKELXEICYDVLGLLDKHLIPKASNPXSKVF 475
           R++SSIEQK E  G+  + ++ KEY  K+++EL  IC D+L +L+KHLIP A++  SKVF
Sbjct: 63  RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122

Query: 476 YLKMKGXYYRYLAXXATGXTXHSVVQDSXKAYQDAFEISKAKMQPTHPITLGLALNFS 649
           Y KMKG YYRYLA  A G         S + Y+ A EI+ A++ PTHPI LGLALNFS
Sbjct: 123 YYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFS 180


>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  198 bits (484), Expect = 4e-52
 Identities = 100/179 (55%), Positives = 127/179 (70%), Gaps = 2/179 (1%)
 Frame = +2

Query: 119 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 298
           MS  +E  V  AKLAEQAERY++M   MK+V  +  +LS EERNLLSVAYKN++GARR+S
Sbjct: 1   MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60

Query: 299 WRVISSIEQKTE--GSERKQQMAKEYXVKVQKELXEICYDVLGLLDKHLIPKASNPXSKV 472
           WR+ISSIEQK E  G+ R+  + KEY  K++ EL +IC+DVL +L+KHLIP A+   SKV
Sbjct: 61  WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120

Query: 473 FYLKMKGXYYRYLAXXATGXTXHSVVQDSXKAYQDAFEISKAKMQPTHPITLGLALNFS 649
           FY KMKG YYRYLA    G         S +AY+ A +I+ A++ PT P+ LGLALNFS
Sbjct: 121 FYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFS 179


>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 542

 Score = 31.5 bits (68), Expect = 0.11
 Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 3/67 (4%)
 Frame = +2

Query: 122 SVDKEELVQRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVAYKNVVGARR 292
           SV    + ++ K  ++ E + ++ + +K V+  ET  E+SN+E N LL + YK V     
Sbjct: 3   SVSNVSVNEQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREF 61

Query: 293 SSWRVIS 313
           S+W   S
Sbjct: 62  STWATFS 68


>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 3131

 Score = 29.5 bits (63), Expect = 0.44
 Identities = 23/104 (22%), Positives = 42/104 (40%), Gaps = 2/104 (1%)
 Frame = +2

Query: 101  PLPSSTMSVDKE--ELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKN 274
            P+ S TMS      E + R +     + Y+ MA  + E  E   ++ +    LLS  Y N
Sbjct: 2997 PIMSITMSDSSAYGEELMRERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056

Query: 275  VVGARRSSWRVISSIEQKTEGSERKQQMAKEYXVKVQKELXEIC 406
                    +R+ + +E+          +  EY   +++ L + C
Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100


>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 580

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +3

Query: 66  FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 188
           FSP +K  + +L LFH  + PS+++    V N  +  SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201


>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
           Usp107|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 695

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 28/110 (25%), Positives = 46/110 (41%), Gaps = 3/110 (2%)
 Frame = +2

Query: 128 DKEELVQRAKLAEQA-ERYDDMAAAMK--EVTETGVELSNEERNLLSVAYKNVVGARRSS 298
           D    ++RA  A QA E+ + +   +K  E+     +L      LL V  + +   R  S
Sbjct: 265 DVRSRIERA--ARQAREKNEKLLQNVKTSEIPINAADLEGINPELLPVIEEEIRSFRDQS 322

Query: 299 WRVISSIEQKTEGSERKQQMAKEYXVKVQKELXEICYDVLGLLDKHLIPK 448
                   +K    +    + KEY  K Q++L +   D+  LL KH I +
Sbjct: 323 ---AMKKREKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369


>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1067

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 10/27 (37%), Positives = 14/27 (51%)
 Frame = +3

Query: 81  KGISELVLFHRPRCPSTRKNWCNVPNW 161
           +G+ +  L    RC +T    CNVP W
Sbjct: 694 EGVRKSALSSLWRCATTYYKVCNVPQW 720


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,532,537
Number of Sequences: 5004
Number of extensions: 45729
Number of successful extensions: 134
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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