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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20090
         (550 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...   134   8e-33
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...   134   8e-33
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...    81   8e-17
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    69   3e-13
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    43   3e-05
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce...    34   0.012
SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu...    29   0.45 
SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual    29   0.45 
SPAC21E11.06 |tif224||translation initiation factor eIF2B delta ...    28   1.0  
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po...    27   2.4  
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc...    26   4.2  
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p...    26   4.2  
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha...    25   5.6  
SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase |S...    25   5.6  
SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom...    25   5.6  
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    25   9.7  
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac...    25   9.7  
SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyce...    25   9.7  
SPCC550.07 |||acetamidase |Schizosaccharomyces pombe|chr 3|||Manual    25   9.7  

>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score =  134 bits (324), Expect = 8e-33
 Identities = 63/85 (74%), Positives = 73/85 (85%)
 Frame = +3

Query: 3   RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182
           RI GPN+ PGKK+DL+ K IQRT+LMMG  +E IED P+GNI GLVGVDQFLVK+GT+TT
Sbjct: 412 RIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPIEDCPAGNIIGLVGVDQFLVKSGTLTT 471

Query: 183 FKNAHNMKVMKFSVSPVVRVAVEPK 257
            + AHNMKVMKFSVSPVV+VAVE K
Sbjct: 472 SEVAHNMKVMKFSVSPVVQVAVEVK 496



 Score =  132 bits (320), Expect = 2e-32
 Identities = 64/85 (75%), Positives = 70/85 (82%)
 Frame = +2

Query: 254 QNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIK 433
           +N  DLPKLVEGLKRL+KSDP V C   ESGEHIVAGAGELHLEICLKDL+EDHA IP+K
Sbjct: 496 KNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLK 555

Query: 434 KSDPVVSYRETVAEESDQLXLSKSP 508
            S PVVSYRE+V+E S    LSKSP
Sbjct: 556 ISPPVVSYRESVSEPSSMTALSKSP 580



 Score = 27.1 bits (57), Expect = 1.8
 Identities = 10/16 (62%), Positives = 13/16 (81%)
 Frame = +1

Query: 499 KVAHKHNRLFMKAQPM 546
           K  +KHNR+FM A+PM
Sbjct: 578 KSPNKHNRIFMTAEPM 593


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score =  134 bits (324), Expect = 8e-33
 Identities = 63/85 (74%), Positives = 73/85 (85%)
 Frame = +3

Query: 3   RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182
           RI GPN+ PGKK+DL+ K IQRT+LMMG  +E IED P+GNI GLVGVDQFLVK+GT+TT
Sbjct: 412 RIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPIEDCPAGNIIGLVGVDQFLVKSGTLTT 471

Query: 183 FKNAHNMKVMKFSVSPVVRVAVEPK 257
            + AHNMKVMKFSVSPVV+VAVE K
Sbjct: 472 SEVAHNMKVMKFSVSPVVQVAVEVK 496



 Score =  132 bits (320), Expect = 2e-32
 Identities = 64/85 (75%), Positives = 70/85 (82%)
 Frame = +2

Query: 254 QNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIK 433
           +N  DLPKLVEGLKRL+KSDP V C   ESGEHIVAGAGELHLEICLKDL+EDHA IP+K
Sbjct: 496 KNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLK 555

Query: 434 KSDPVVSYRETVAEESDQLXLSKSP 508
            S PVVSYRE+V+E S    LSKSP
Sbjct: 556 ISPPVVSYRESVSEPSSMTALSKSP 580



 Score = 27.1 bits (57), Expect = 1.8
 Identities = 10/16 (62%), Positives = 13/16 (81%)
 Frame = +1

Query: 499 KVAHKHNRLFMKAQPM 546
           K  +KHNR+FM A+PM
Sbjct: 578 KSPNKHNRIFMTAEPM 593


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1000

 Score = 81.4 bits (192), Expect = 8e-17
 Identities = 41/81 (50%), Positives = 52/81 (64%)
 Frame = +2

Query: 260 PADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKS 439
           P ++ KLV GL  L ++DP VQ   EE+GEH++  AGE+HLE CLKDL E  A I I+ S
Sbjct: 573 PFEMNKLVTGLDMLNQADPCVQIAVEENGEHVIMCAGEIHLERCLKDLRERFAKIEIQAS 632

Query: 440 DPVVSYRETVAEESDQLXLSK 502
            P+V YRET     D L  +K
Sbjct: 633 QPLVPYRETTIATPDLLAKNK 653



 Score = 54.4 bits (125), Expect = 1e-08
 Identities = 27/84 (32%), Positives = 48/84 (57%), Gaps = 1/84 (1%)
 Frame = +3

Query: 6   IMGPNFTPGKKEDLYEK-TIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182
           + GP + P   E    K T++   LMMG+ +  +E VP+GN+  + G+   +++T T+ +
Sbjct: 487 VYGPKYDPVNPEKHITKVTVESLYLMMGQELVYLETVPAGNVFAIGGLAGTVLRTATLCS 546

Query: 183 FKNAHNMKVMKFSVSPVVRVAVEP 254
             N  N+  +   + P+VRVA+EP
Sbjct: 547 SPNGPNLVGVTQQMEPIVRVALEP 570


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 983

 Score = 69.3 bits (162), Expect = 3e-13
 Identities = 34/84 (40%), Positives = 52/84 (61%)
 Frame = +2

Query: 257 NPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKK 436
           NP++LPKL++GL++  KS P+     EESGEH + G GE++++  L DL   ++ I I+ 
Sbjct: 609 NPSELPKLLDGLRKTNKSYPLSITKVEESGEHTIFGTGEMYMDCLLYDLRTLYSEIEIRV 668

Query: 437 SDPVVSYRETVAEESDQLXLSKSP 508
           SDPV  + ET  + S     S +P
Sbjct: 669 SDPVARFCETAVDTSSIKCFSDTP 692



 Score = 32.7 bits (71), Expect = 0.037
 Identities = 22/87 (25%), Positives = 41/87 (47%), Gaps = 3/87 (3%)
 Frame = +3

Query: 3   RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182
           +++G N++   +ED+    I    +   RY   ++   +G +  L GVD  + KT TI +
Sbjct: 521 KVLGENYSLEDEEDMVVAHIAEICVPCARYRLHVDGAVAGMLVLLGGVDNSISKTATIVS 580

Query: 183 FK---NAHNMKVMKFSVSPVVRVAVEP 254
                + +  + +      V +VAVEP
Sbjct: 581 DNLKDDPYIFRPIAHMSESVFKVAVEP 607


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 43.2 bits (97), Expect = 3e-05
 Identities = 21/72 (29%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
 Frame = +2

Query: 266 DLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSD 442
           D     + L R  + DP  +  ++ ES E I++G GELHLE+ ++ +  ++  +  +   
Sbjct: 486 DTTSFSKALNRFQREDPTFRVQLDNESKETIISGMGELHLEVYVERMRREYK-VDCETGK 544

Query: 443 PVVSYRETVAEE 478
           P V++RET++++
Sbjct: 545 PRVAFRETLSKK 556


>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 813

 Score = 34.3 bits (75), Expect = 0.012
 Identities = 20/73 (27%), Positives = 36/73 (49%), Gaps = 1/73 (1%)
 Frame = +2

Query: 266 DLPKLVEGLKRLAKSDPMVQCINE-ESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSD 442
           D P L+E L  + + DP  +   + E+G+ ++ G G +HL++  + L  +          
Sbjct: 456 DEPALLEALANMNREDPSFRYTQDLENGQLLIQGMGIMHLQVSYERLVSEFGA-RASLGK 514

Query: 443 PVVSYRETVAEES 481
             V YRET+ + S
Sbjct: 515 VQVGYRETLIDVS 527


>SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit
           Tfg3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 241

 Score = 29.1 bits (62), Expect = 0.45
 Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 6/49 (12%)
 Frame = +2

Query: 260 PADLPKLVEGLKRLAKSD--PMVQCINEESGEHIVA----GAGELHLEI 388
           P D+ KL EGL++L + D   +VQ +NE     +        GE H+++
Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDL 220


>SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 973

 Score = 29.1 bits (62), Expect = 0.45
 Identities = 19/56 (33%), Positives = 27/56 (48%)
 Frame = -2

Query: 477 SSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPATMCSPDSSLIHCTMGS 310
           S  T   YDTTG+D ++     SS R       WS+  P    +P ++ I+ T GS
Sbjct: 117 SDYTSVYYDTTGTDDVVYEYHPSSERHKSTNDTWSTNLPT---NPTTTAIYSTSGS 169


>SPAC21E11.06 |tif224||translation initiation factor eIF2B delta
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score = 27.9 bits (59), Expect = 1.0
 Identities = 12/33 (36%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
 Frame = +3

Query: 138 VGVDQFLVKTGTITTFKNAHNMKV--MKFSVSP 230
           +GVD F  K G +  +K+  N+K+  +K+ V+P
Sbjct: 404 MGVDDFEEKPGVLANWKSVKNLKLLSLKYDVTP 436


>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 793

 Score = 26.6 bits (56), Expect = 2.4
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = +3

Query: 147 DQFLVKTGTITTFKNAHNMKVMKFSVSPVV 236
           D FLVK G +   ++ +N+    FSVS +V
Sbjct: 82  DTFLVKYGDLINIQDPYNLNKTLFSVSDLV 111


>SPCC622.12c |||NADP-specific glutamate dehydrogenase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 451

 Score = 25.8 bits (54), Expect = 4.2
 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
 Frame = +3

Query: 12  GPNFTP-GKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVG 143
           G +F P GK ++   +  Q  +  + RY+    DVP+G+I G+ G
Sbjct: 115 GSDFDPKGKSDNEIRRFSQAFMRQLFRYIGPQTDVPAGDI-GVTG 158


>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1323

 Score = 25.8 bits (54), Expect = 4.2
 Identities = 13/41 (31%), Positives = 22/41 (53%)
 Frame = +2

Query: 383 EICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLXLSKS 505
           E+C  +LE+D     I +S   +S +   A++ DQL  S +
Sbjct: 64  ELCNFELEKDSLESMIHESSTALSAQSNTAQDGDQLASSST 104


>SPAC23E2.03c |ste7||meiotic suppressor protein
           Ste7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 569

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 10/33 (30%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
 Frame = -2

Query: 375 SSPAPATMCSPDSSL--IHCTMGSDLARRLRPS 283
           S+ +P+T+CSPD+++  ++   G  L  ++ P+
Sbjct: 403 SNVSPSTICSPDNNVTFVNLAPGEKLTFKIEPN 435


>SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 345

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +1

Query: 214 NSVYHQSCVSLLSPKP 261
           NSV  +SCVS+LS KP
Sbjct: 172 NSVSKKSCVSILSRKP 187


>SPAC3C7.09 |set8||lysine methyltransferase Set8
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 429

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 15/52 (28%), Positives = 26/52 (50%)
 Frame = +2

Query: 194 PQHEGDEIQCITSRACRC*AQNPADLPKLVEGLKRLAKSDPMVQCINEESGE 349
           P+ EGD I  +        + +  DL K     +R  K+ PM++ +++ SGE
Sbjct: 248 PEPEGDRITNVMKLLIPEDSNDSLDLAK-----RRSCKTPPMIEFVSDSSGE 294


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
           E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 15/56 (26%), Positives = 26/56 (46%), Gaps = 2/56 (3%)
 Frame = +3

Query: 90  YVEAIEDVPSGNIC--GLVGVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVE 251
           Y+ A  ++ SG     G V +   L  T  ++    A  + + K + SP+VR+  E
Sbjct: 414 YIAAFSEILSGRNYDFGEVSIQLMLSCTTCVSNMMEALPLCMAKIAYSPIVRILCE 469


>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
           Smc6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1140

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 9/24 (37%), Positives = 14/24 (58%)
 Frame = -3

Query: 116 GHILNSFNITSHHKDCTLDSLLIQ 45
           G+++N F + SHH    L  L+ Q
Sbjct: 573 GNVINGFIVRSHHDQLILKELMRQ 596


>SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 351

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 11/39 (28%), Positives = 19/39 (48%)
 Frame = +2

Query: 317 MVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIK 433
           M + IN +S         +  +E CL  L+E H  +P++
Sbjct: 1   MKEMINLDSSSEPSIVTKQFSVEECLSKLKEQHCYVPMR 39


>SPCC550.07 |||acetamidase |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 533

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = +2

Query: 293 KRLAKSDPMVQCINEESGEHIVAGAGEL 376
           KR A +  +V C+NE   E  +A A EL
Sbjct: 83  KRAAIAQQLVNCVNELFYEEALARAAEL 110


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,323,055
Number of Sequences: 5004
Number of extensions: 46732
Number of successful extensions: 154
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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