SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120082.Seq
         (746 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc...    30   0.30 
SPBC16G5.01 |rpn12|mts3, SPBC342.07|19S proteasome regulatory su...    28   1.2  
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p...    27   2.1  
SPBC3H7.10 |||elongator homolog|Schizosaccharomyces pombe|chr 2|...    27   3.8  
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces...    26   6.6  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    25   8.7  
SPAC139.02c |oac1||anion transporter|Schizosaccharomyces pombe|c...    25   8.7  

>SPCC132.04c |||NAD-dependent glutamate dehydrogenase
            |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1106

 Score = 30.3 bits (65), Expect = 0.30
 Identities = 17/57 (29%), Positives = 33/57 (57%), Gaps = 3/57 (5%)
 Frame = +2

Query: 251  DYSKHKKFVYWPKDTNALVPLVWRESKEIKLPYKTLSHNLS-KIIKV--YVYQHDKI 412
            DY    K +   ++ N     +W+   E K+PY +LS++LS +I+K+   +Y ++K+
Sbjct: 975  DYVNEVKRII-QRNANLEFEAIWKGHSENKIPYTSLSNHLSTEIVKLDHDIYNYEKL 1030


>SPBC16G5.01 |rpn12|mts3, SPBC342.07|19S proteasome regulatory
           subunit Rpn12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 270

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 16/64 (25%), Positives = 31/64 (48%), Gaps = 3/64 (4%)
 Frame = +2

Query: 236 IKREKDYSKHKKFVYWPKDTNALVP---LVWRESKEIKLPYKTLSHNLSKIIKVYVYQHD 406
           +  E+ +      +Y+PK+ NAL     ++  E  E++LP     H +S I ++  Y  +
Sbjct: 206 LAEERGWDIRDGVIYFPKEANALETEDGMLIDEEDELELPPTASKHTISSIRQLLSYTSE 265

Query: 407 KIEI 418
             +I
Sbjct: 266 LEQI 269


>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1040

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 11/40 (27%), Positives = 23/40 (57%)
 Frame = +3

Query: 54  GNFMIEKEISYSINFSQDLLYKILNSYIVPNYSLAQQYFD 173
           G+F+  + +++ I  S+D+  +  N  I P  S+ ++Y D
Sbjct: 763 GHFLCRECLTHVITSSEDMAKQTSNENISPKCSVCEEYID 802


>SPBC3H7.10 |||elongator homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 249

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 27/131 (20%), Positives = 54/131 (41%), Gaps = 3/131 (2%)
 Frame = +2

Query: 164 IFRFVRRKRLSHSYTYSERLQ*HNIKREK---DYSKHKKFVYWPKDTNALVPLVWRESKE 334
           ++  ++ K  +   T+S+ L  H I   K   D    K F +    +    P+    SK 
Sbjct: 36  LYHALKAKESTCFLTFSKTLDEHAISMRKWGMDIKTKKNFFFIDGFSMLFAPI----SKP 91

Query: 335 IKLPYKTLSHNLSKIIKVYVYQHDKIEIKFEHVYFSKSDIDLFDSTMANKISKLLTLLKM 514
            K+      +++  +    +   ++ + +FE+      DID+  ST A   +K    ++ 
Sbjct: 92  SKVQAPETKNHIKSVFAPVIQCVEENDFEFENSTIIIEDIDILQSTHALDSTK----IQQ 147

Query: 515 ETLHRRRCKTR 547
             L  R+C +R
Sbjct: 148 AILELRKCFSR 158


>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1639

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 12/35 (34%), Positives = 17/35 (48%)
 Frame = -2

Query: 727  GAILSINVVSGTNGDMFCASVNASMSAIMWHITFS 623
            G ++S  +V   NG M C S     +  MW  TF+
Sbjct: 1188 GLVISKELVELHNGSMSCQSRRGVGTRFMWTATFT 1222


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
            Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 11/36 (30%), Positives = 22/36 (61%)
 Frame = +2

Query: 458  LFDSTMANKISKLLTLLKMETLHRRRCKTRKWAAMK 565
            +F+ T+ + +   LTL +++ L   +CKT K  ++K
Sbjct: 2115 VFEETLVDNLYHQLTLYQLQDLICSKCKTVKQWSLK 2150


>SPAC139.02c |oac1||anion transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 320

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 11/40 (27%), Positives = 18/40 (45%)
 Frame = -2

Query: 529 PMKRFHFQQSQQFGYLVRHRGIK*INVTFRKIYMFKFDFN 410
           P KR +    Q F  + RH GI+ +       Y+++   N
Sbjct: 62  PSKRIYKSVGQAFSLIARHEGIRGLQRGLGTAYVYQICLN 101


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,008,344
Number of Sequences: 5004
Number of extensions: 60492
Number of successful extensions: 189
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -