Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV120073.Seq
         (650 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC18H10.03 |tif35||translation initiation factor eIF3g|Schizos...    54   2e-08
SPAC23A1.09 |||RNA-binding protein|Schizosaccharomyces pombe|chr...    40   4e-04
SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces po...    34   0.020
SPAC19A8.13 |usp101|snp1|U1 snRNP-associated protein Usp101 |Sch...    32   0.062
SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual      31   0.14 
SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces...    29   0.44 
SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce...    29   0.44 
SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar...    29   0.58 
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    27   2.3  
SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc...    26   4.1  
SPAC1610.03c |crp79|meu5|poly|Schizosaccharomyces pombe|chr 1|||...    26   5.4  
SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccha...    26   5.4  
SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyc...    26   5.4  
SPBC2D10.11c |||nucleosome assembly protein Nap2 |Schizosaccharo...    26   5.4  
SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha...    25   7.2  

>SPBC18H10.03 |tif35||translation initiation factor
           eIF3g|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 282

 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 31/84 (36%), Positives = 50/84 (59%), Gaps = 8/84 (9%)
 Frame = +2

Query: 2   GLKIVTEYKYDNDNKKVKIVRTYKIEKCVVSK----SIAKRKTWSKFGDSASDKPGPNPA 169
           G K + E++ D++ KKVK+ R   I K V+++    ++A+RK W KFG  A    G +  
Sbjct: 30  GTKTMIEFRIDDNGKKVKVTRV--IRKTVITERVQHAVAERKKWKKFGKEAGKNSGVDAR 87

Query: 170 TTNVAEDVFMQ----FITSKEESQ 229
           TT+V E+V ++    + T+KEE Q
Sbjct: 88  TTSVGENVQLRLQLGWTTTKEEEQ 111



 Score = 45.2 bits (102), Expect = 8e-06
 Identities = 20/44 (45%), Positives = 29/44 (65%)
 Frame = +3

Query: 519 EADIDDLVKGFGPVHKLYLAKEKSTGHCKGFAYVHFKFRADAAK 650
           E ++ DL + FG + ++YLAK+K TG  KGFA+V +  R  A K
Sbjct: 215 EEELRDLFRRFGGIQRVYLAKDKETGRAKGFAFVSYYDRDCAIK 258



 Score = 35.9 bits (79), Expect = 0.005
 Identities = 24/91 (26%), Positives = 38/91 (41%), Gaps = 7/91 (7%)
 Frame = +1

Query: 256 LKPPSSNVIFKCRTCQGDHLTLYCPFKH----TQIXXXXXXXXXXXXXXXXXXSNKYIPP 423
           +K   S+V+ +CR C+G+H T  CP+K                            +YI P
Sbjct: 121 VKAKGSSVV-RCRACKGNHFTAQCPYKSIIGPVDEPPLDASPVSSRASGALGEKGRYIAP 179

Query: 424 ---SSAGRIPGRDQPPVRREDVTAIRISNLS 507
              + +GR  G       R+D   +R++NLS
Sbjct: 180 HLRAGSGRESGDSMFKRERDDSATLRVTNLS 210


>SPAC23A1.09 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 121

 Score = 39.5 bits (88), Expect = 4e-04
 Identities = 17/46 (36%), Positives = 26/46 (56%)
 Frame = +3

Query: 513 AVEADIDDLVKGFGPVHKLYLAKEKSTGHCKGFAYVHFKFRADAAK 650
           A E  ++DL   FGPV  L+L  ++ TG+ KG+A + +     A K
Sbjct: 21  ATEEQVEDLFADFGPVKNLHLNLDRRTGYVKGYALIEYATLEQAQK 66


>SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 833

 Score = 33.9 bits (74), Expect = 0.020
 Identities = 15/44 (34%), Positives = 25/44 (56%)
 Frame = +3

Query: 519 EADIDDLVKGFGPVHKLYLAKEKSTGHCKGFAYVHFKFRADAAK 650
           E D+  L   FG + ++++  +K T + KGFAY+ F    DA +
Sbjct: 336 EDDLKSLFGPFGQLEQVHMPIDKKTNNPKGFAYIDFHDADDAVR 379


>SPAC19A8.13 |usp101|snp1|U1 snRNP-associated protein Usp101
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 261

 Score = 32.3 bits (70), Expect = 0.062
 Identities = 13/41 (31%), Positives = 24/41 (58%)
 Frame = +3

Query: 519 EADIDDLVKGFGPVHKLYLAKEKSTGHCKGFAYVHFKFRAD 641
           E+DI+     +GP+ ++ + + K TG   G+A+V F+   D
Sbjct: 113 ESDIEREFTRYGPIERIRVVRNKVTGKSMGYAFVVFERERD 153


>SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 31.1 bits (67), Expect = 0.14
 Identities = 12/25 (48%), Positives = 18/25 (72%)
 Frame = +3

Query: 552 GPVHKLYLAKEKSTGHCKGFAYVHF 626
           G V+++ +  +K TGH KGFAY+ F
Sbjct: 79  GSVNRVTILCDKFTGHPKGFAYIEF 103


>SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 369

 Score = 29.5 bits (63), Expect = 0.44
 Identities = 12/36 (33%), Positives = 20/36 (55%)
 Frame = +3

Query: 519 EADIDDLVKGFGPVHKLYLAKEKSTGHCKGFAYVHF 626
           +AD+ +     G + ++ L   + TG CKGF +V F
Sbjct: 241 DADLKEHFGQVGQIRRVRLMTFEDTGKCKGFGFVDF 276


>SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 604

 Score = 29.5 bits (63), Expect = 0.44
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = +3

Query: 525 DIDDLVKGFGPVHKLYLAKEKSTGHCKGFAYVHF 626
           ++ D  +  GPV    + ++K +G  KG AYV F
Sbjct: 255 ELYDFFEQAGPVRDAQIVRDKISGRSKGVAYVEF 288


>SPAC57A7.04c |pabp||mRNA export shuttling protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 653

 Score = 29.1 bits (62), Expect = 0.58
 Identities = 14/44 (31%), Positives = 22/44 (50%)
 Frame = +3

Query: 519 EADIDDLVKGFGPVHKLYLAKEKSTGHCKGFAYVHFKFRADAAK 650
           EA + +L    GPV  + + ++  T    G+AYV+F    D  K
Sbjct: 93  EAMLFELFNSIGPVASIRVCRDAVTRRSLGYAYVNFHNMEDGEK 136


>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 422

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 13/43 (30%), Positives = 20/43 (46%)
 Frame = +3

Query: 519 EADIDDLVKGFGPVHKLYLAKEKSTGHCKGFAYVHFKFRADAA 647
           E  + D+ K  GPV    L  +  +G  KG+ +  +   A AA
Sbjct: 18  EEQVIDIFKQSGPVKSFQLVIDPESGQPKGYGFCEYHDPATAA 60


>SPAC328.05 |||RNA-binding protein involved in export of
           mRNAs|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 434

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 16/38 (42%), Positives = 19/38 (50%)
 Frame = +3

Query: 534 DLVKGFGPVHKLYLAKEKSTGHCKGFAYVHFKFRADAA 647
           DL    G V +  +A E  TG  KGF  V F+   DAA
Sbjct: 298 DLFTDIGSVIRARIAYEP-TGRSKGFGVVQFENENDAA 334


>SPAC1610.03c |crp79|meu5|poly|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 710

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 11/37 (29%), Positives = 21/37 (56%)
 Frame = +3

Query: 519 EADIDDLVKGFGPVHKLYLAKEKSTGHCKGFAYVHFK 629
           ++ ++DL   FG +    LA   ++G  KG+ +V F+
Sbjct: 397 KSQLEDLFSPFGSILSSMLACYPNSGISKGYGFVAFR 433


>SPAC20G8.08c |fft1||fun thirty related protein
           Fft1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 944

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 14/40 (35%), Positives = 18/40 (45%)
 Frame = +2

Query: 41  NKKVKIVRTYKIEKCVVSKSIAKRKTWSKFGDSASDKPGP 160
           NK  + +    IEK  V K  AKRK + + G     K  P
Sbjct: 172 NKSAQKLNNQPIEKSSVDKENAKRKRYVEEGTKQGQKKKP 211


>SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 438

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
 Frame = +3

Query: 507 HFAVEADIDDLVKGFGP---VHKLYLAKEKSTGHCKGFAYVHFK 629
           + A EA+ + L + FG    +  + + ++  T   KGFAY+ FK
Sbjct: 276 NLAFEAEEEPLWRYFGDCGSIDYVRIVRDPKTNLGKGFAYIQFK 319


>SPBC2D10.11c |||nucleosome assembly protein Nap2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 379

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 15/60 (25%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
 Frame = +2

Query: 110 RKTWSKFG-DSASDKPGPNPATTNVAEDVFMQFITSKEESQRPDDGELDVLSHHPAMLSL 286
           +K+W+ F   +  + P  +   +  A +   Q + S+ E +   D    +L ++PAMLS+
Sbjct: 9   KKSWNGFAAQTPQNTPSSDVHLSKAALEKARQTLQSQLEDKSAHDEVSGLLRNNPAMLSM 68


>SPBC660.15 |||mRNA cleavage factor complex subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 474

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 10/32 (31%), Positives = 18/32 (56%)
 Frame = +3

Query: 534 DLVKGFGPVHKLYLAKEKSTGHCKGFAYVHFK 629
           D  + FG V    + ++ +TG  +GF ++ FK
Sbjct: 181 DYFEQFGEVLDCTVMRDSTTGRSRGFGFLTFK 212


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,394,701
Number of Sequences: 5004
Number of extensions: 43461
Number of successful extensions: 114
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)