Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20069
(636 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 164 6e-41
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 130 7e-31
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 129 2e-30
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 127 7e-30
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 87 1e-17
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 48 8e-06
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 36 0.028
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 36 0.028
SB_8282| Best HMM Match : NHL (HMM E-Value=9.2e-23) 32 0.45
SB_42147| Best HMM Match : Thioredoxin (HMM E-Value=0.3) 29 4.2
SB_23535| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.5
SB_45801| Best HMM Match : DUF577 (HMM E-Value=3.8) 28 5.5
SB_44193| Best HMM Match : 7tm_1 (HMM E-Value=4.4e-12) 28 7.3
SB_21445| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7
SB_9096| Best HMM Match : Acid_phosphat_A (HMM E-Value=0.18) 27 9.7
SB_493| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7
SB_44478| Best HMM Match : Na_H_Exchanger (HMM E-Value=0) 27 9.7
SB_34372| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7
SB_33710| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7
SB_23753| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 164 bits (398), Expect = 6e-41
Identities = 75/87 (86%), Positives = 83/87 (95%)
Frame = +2
Query: 245 RQEEKDKDIGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGD 424
R+E+++K +GTVIGIDLGTTYSCVGV+KNGRVEIIANDQGNRITPSYVAFT++GERLIGD
Sbjct: 693 RKEQREK-VGTVIGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGD 751
Query: 425 AAKNQLTTNPENTVFDAKRLIGREWSD 505
AKNQLTTNPENTVFDAKRLIGR W D
Sbjct: 752 GAKNQLTTNPENTVFDAKRLIGRSWDD 778
Score = 65.3 bits (152), Expect = 4e-11
Identities = 30/43 (69%), Positives = 35/43 (81%)
Frame = +1
Query: 508 TVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVL 636
+VQ+D+KFFPFKVV+KN KPHVQV+ K FA EEISAMVL
Sbjct: 780 SVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAMVL 822
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 130 bits (315), Expect = 7e-31
Identities = 60/75 (80%), Positives = 67/75 (89%)
Frame = +2
Query: 281 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 460
IGIDLGTTYSCVGV++NG+VEIIANDQGNR TPSYV FT D ERL+GDAAKNQ+ NPEN
Sbjct: 7 IGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFT-DTERLVGDAAKNQVALNPEN 65
Query: 461 TVFDAKRLIGREWSD 505
T+FDAKRLIGR + D
Sbjct: 66 TIFDAKRLIGRRYDD 80
Score = 52.0 bits (119), Expect = 4e-07
Identities = 23/43 (53%), Positives = 31/43 (72%)
Frame = +1
Query: 508 TVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVL 636
TVQ D+K +PFK++ N+KP +QV+ K FA EEIS+MVL
Sbjct: 82 TVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSMVL 124
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 129 bits (311), Expect = 2e-30
Identities = 60/75 (80%), Positives = 69/75 (92%)
Frame = +2
Query: 281 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 460
+GIDLGTTYSCVGV+++G+VEIIANDQGNR TPSYVAF+ D ERLIGDAAKNQ+ NPEN
Sbjct: 7 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFS-DTERLIGDAAKNQVAMNPEN 65
Query: 461 TVFDAKRLIGREWSD 505
TVFDAKRLIGR++ D
Sbjct: 66 TVFDAKRLIGRKFDD 80
Score = 43.2 bits (97), Expect = 2e-04
Identities = 19/44 (43%), Positives = 27/44 (61%)
Frame = +1
Query: 505 PTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVL 636
P V D+ +PF V+ + +P +QV+ K F PEEIS+MVL
Sbjct: 81 PVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSMVL 124
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 127 bits (307), Expect = 7e-30
Identities = 59/75 (78%), Positives = 67/75 (89%)
Frame = +2
Query: 281 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 460
IGIDLGTTYSCVGV+++G+VEIIANDQGNR TPSYVAFT D ERL+GDAAKNQ+ NP N
Sbjct: 7 IGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DEERLVGDAAKNQVAMNPTN 65
Query: 461 TVFDAKRLIGREWSD 505
T+FDAKRLIGR + D
Sbjct: 66 TIFDAKRLIGRRFDD 80
Score = 31.5 bits (68), Expect = 0.59
Identities = 16/36 (44%), Positives = 22/36 (61%)
Frame = +1
Query: 529 FFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVL 636
FF +VV + +P V+V+ K F EEIS+MVL
Sbjct: 114 FFAEEVVNEAGRPKVKVEYKGETKTFFAEEISSMVL 149
Score = 31.1 bits (67), Expect = 0.78
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +1
Query: 505 PTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEI 621
P V+ D+K + F+VV + +P V+V+ K F EE+
Sbjct: 81 PGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEV 119
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 87.0 bits (206), Expect = 1e-17
Identities = 39/79 (49%), Positives = 59/79 (74%), Gaps = 2/79 (2%)
Frame = +2
Query: 278 VIGIDLGTTYSCVGVYK--NGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 451
+IGIDLGTTYSCVG Y+ +GR++I +++ +++ PS VAFT G L+G+ A +Q N
Sbjct: 33 IIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGV-LVGEKALSQAEIN 91
Query: 452 PENTVFDAKRLIGREWSDQ 508
P+NT++DAKR IG+ ++ Q
Sbjct: 92 PKNTIYDAKRFIGKTFTKQ 110
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 47.6 bits (108), Expect = 8e-06
Identities = 22/55 (40%), Positives = 35/55 (63%)
Frame = +2
Query: 275 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQ 439
+V+G D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 2 SVVGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSL-GDKQRLIGTSGKNQ 55
Score = 37.1 bits (82), Expect = 0.012
Identities = 18/44 (40%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
Frame = +1
Query: 505 PTVQHDVKFFPFKVVE-KNSKPHVQVQTSQGDKVFAPEEISAMV 633
P VQ ++ P+KVVE N +QVQ +VF+PE++ AM+
Sbjct: 137 PAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAML 180
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 35.9 bits (79), Expect = 0.028
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = +2
Query: 329 NGRVEIIANDQGNRITPSYVAFTQDGERLI 418
+G IIAND G+R+TP+ VAF+ D E+LI
Sbjct: 82 DGNTTIIANDAGDRVTPAMVAFS-DTEKLI 110
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 35.9 bits (79), Expect = 0.028
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = +2
Query: 341 EIIANDQGNRITPSYVAFTQDGERL 415
++I N +G+R TPS VAFT +GERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_8282| Best HMM Match : NHL (HMM E-Value=9.2e-23)
Length = 877
Score = 31.9 bits (69), Expect = 0.45
Identities = 18/54 (33%), Positives = 29/54 (53%)
Frame = +2
Query: 314 VGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDA 475
V Y N +V + A D G+++T Y A T +G+ L+ A + +T N + V A
Sbjct: 728 VSDYHNRKVHVFARDDGSQVT-CYTAVTDEGDGLLN--APSGITVNSKGEVIVA 778
>SB_42147| Best HMM Match : Thioredoxin (HMM E-Value=0.3)
Length = 787
Score = 28.7 bits (61), Expect = 4.2
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = -1
Query: 498 HSRPIRRLASKTVFSGLVVSWF 433
H I RL+S+ VFSG V+ WF
Sbjct: 114 HKILIDRLSSRFVFSGTVIQWF 135
>SB_23535| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 222
Score = 28.3 bits (60), Expect = 5.5
Identities = 17/70 (24%), Positives = 34/70 (48%)
Frame = +2
Query: 413 LIGDAAKNQLTTNPENTVFDAKRLIGREWSDQLYNMMLSSSPSRSLKRTANLMFKYKLHK 592
++ D+AK++ T EN + D R+WS + +RSL+ L+ + +
Sbjct: 1 MVQDSAKDEKMTEAENDIDD------RKWSKPVMEKRRRERINRSLEELKRLVLEAQHRD 54
Query: 593 ATKYLLLKKS 622
++Y L+K+
Sbjct: 55 CSRYTKLEKA 64
>SB_45801| Best HMM Match : DUF577 (HMM E-Value=3.8)
Length = 443
Score = 28.3 bits (60), Expect = 5.5
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = -1
Query: 555 LFNDLEGEELNIMLYSWSLH 496
LF LEG+ +NI L +WSLH
Sbjct: 358 LFKFLEGKLININLRNWSLH 377
>SB_44193| Best HMM Match : 7tm_1 (HMM E-Value=4.4e-12)
Length = 863
Score = 27.9 bits (59), Expect = 7.3
Identities = 19/70 (27%), Positives = 32/70 (45%)
Frame = +2
Query: 107 DLIVAVNIILFRFLLISESFNL*ILHGQDAVEFVRASFGGRGRLCRRQEEKDKDIGTVIG 286
+L V N++L L +++ ++ AV RA F G+LCR + DI +
Sbjct: 141 ELRVPANLLLVS-LAANDALTGLVIQPSHAVSLYRALFQPYGKLCRTDGKIFIDICAMFS 199
Query: 287 IDLGTTYSCV 316
+ G T C+
Sbjct: 200 LTSGLTSLCL 209
>SB_21445| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 122
Score = 27.5 bits (58), Expect = 9.7
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = +1
Query: 286 YRFGYHLLMCRCLQKWTCRNHCQ*PGK 366
Y +L RCL + TC HC P K
Sbjct: 63 YDIHQEILRARCLNEGTCVEHCDSPKK 89
>SB_9096| Best HMM Match : Acid_phosphat_A (HMM E-Value=0.18)
Length = 417
Score = 27.5 bits (58), Expect = 9.7
Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +2
Query: 251 EEKDKDIGTVIGIDLGT-TYSCVGVYKNGRVEIIANDQGN 367
E +D+D G V G+DL T T Y +G + I N Q N
Sbjct: 180 ESQDRDTGDVAGVDLSTRTEQKNAKYADGYEKEIENAQTN 219
>SB_493| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 113
Score = 27.5 bits (58), Expect = 9.7
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +2
Query: 509 LYNMMLSSSPSRSLKRTANLMFKYKLHKATKYLLL 613
L+ + + SP+RS + N+MF Y L + +L L
Sbjct: 37 LFRKLTNHSPARSSIESGNMMFHYILENSVCFLTL 71
>SB_44478| Best HMM Match : Na_H_Exchanger (HMM E-Value=0)
Length = 557
Score = 27.5 bits (58), Expect = 9.7
Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = -1
Query: 312 HE*VVPKSIPITVPMSLSFSSCRRHKRPRPPKLARTNSTAS*PCKIY-RLKDSEINKNLN 136
HE V I + V + +S HK+P K+ N+ + P ++ + +E NL
Sbjct: 94 HETGVAMIIGVVVGALIKYSEVGHHKQPLSVKIKNCNNLTNAPQSVWVNINGTEYTYNLM 153
Query: 135 KIIFTA 118
I F A
Sbjct: 154 GIRFPA 159
>SB_34372| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 252
Score = 27.5 bits (58), Expect = 9.7
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +2
Query: 509 LYNMMLSSSPSRSLKRTANLMFKYKLHKATKYLLL 613
L+ + + SP+RS + N+MF Y L + +L L
Sbjct: 37 LFRKLTNHSPARSSIESGNMMFHYILENSVCFLTL 71
>SB_33710| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 96
Score = 27.5 bits (58), Expect = 9.7
Identities = 18/50 (36%), Positives = 24/50 (48%)
Frame = -1
Query: 159 SEINKNLNKIIFTATIRSL*NAFIKHLRIQTVHRYFSFNCSRKTRDSSLL 10
S +N NL + ++TIRSL A LR F RK RD+ +L
Sbjct: 12 SGVNDNLEVLPSSSTIRSLFEAKFCPLRPSPGGLVFRLRVERKLRDTGVL 61
>SB_23753| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 517
Score = 27.5 bits (58), Expect = 9.7
Identities = 25/96 (26%), Positives = 37/96 (38%), Gaps = 1/96 (1%)
Frame = -1
Query: 480 RLASKTVFSGLVVSWFLAASPIKRSPS*VKATYEGVM-RFPWSLAMISTRPFL*TPTHE* 304
RL T G ++ AS + + + AT ++ P S T+P TP HE
Sbjct: 349 RLELYTCKQGAAITTSTTASSVATTVTTTTATSSSMLPNTPSSSPSTGTQPSS-TPIHEP 407
Query: 303 VVPKSIPITVPMSLSFSSCRRHKRPRPPKLARTNST 196
+ +T+ S S H RP K T+ T
Sbjct: 408 PTQGTTLVTMETSTSHLKSSHHSRPTTRKTLFTSKT 443
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,276,283
Number of Sequences: 59808
Number of extensions: 431971
Number of successful extensions: 1008
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 931
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1001
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1596754500
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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